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sPEED:an integrated,smartphone-operated,handheld digital PCR Device for point-of-care testing

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Microsystems & nanoengineering 2024年第3期10卷 149-158页

作者： Haoqing Zhang Xiaocheng Liu Xinlu Wang Zhiqiang Yan Ying Xu Martina Gaňová TomášŘezníček Marie Korabečná Pavel neuzil Ministry of Education Key Laboratory of Micro and nano systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072PR China The Key Laboratory of Biomedical Information Engineering of the Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049PR China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049PR China school of Marine science and Technology Northwestern Polytechnical UniversityXi’anShaanxi 710072PR China Central European Institute of Technology Brno University of TechnologyPurkyňova 12361300 BrnoCzech Republic ITD Tech s.R.O Osvoboditelu100573581 BohumínCzech Republic Institute of Biology and Medical Genetics First Faculty of MedicineCharles University and General University Hospital of PragueAlbertov 412800 PragueCzech Republic

This study elaborates on the design,fabrication,and data analysis details of sPEED,a recently proposed smartphonebased digital polymerase chain reaction(dPCR)*** dPCR chips incorporate partition diameters ranging from 50μm to 5μm,and these partitions are organized into six distinct blocks to facilitate image *** to the superior thermal conductivity of si and its potential for mass production,the dPCR chips were fabricated on a si substrate.A temperature control system based on a high-power density Peltier element and a preheating/cooling PCR protocol user interface shortening the thermal cycle *** optical design employs four 470 nm light-emitting diodes as light sources,with filters and mirrors effectively managing the light emitted during *** algorithm is utilized for image processing and illumination nonuniformity correction including conversion to a monochromatic format,partition identification,skew correction,and the generation of an image correction *** validated the device using a range of deoxyribonucleic acid targets,demonstrating its potential applicability across multiple ***,we provide guidance and verification of the design and testing of the recently proposed sPEED device.

关键词： correction smart testing

Is It about a Bicycle? A Posthumanist Perspective on the Role of Women’s Bicycles in WWII Resistance Movements

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sociology study 2024年第4期14卷 198-211页

作者： Zuzana Križalkovičová Academy of Fine Arts Vienna Austria

Drawing upon Donna Haraway’s cyborg theory,this paper argues that the bicycle can be conceived as a cyborg extension of body and identity which transcended its mechanical essence to become an integral co-partner for women involved in resistance activities in *** specifically,the study explores how women used cycling during World War Ⅱ not only for mobility but as a profound expression of courage,endurance and solidarity which enabled them to overcome physical and symbolic barriers under totalitarian *** bicycle emerges not only as a central co-agent but also actively co-creates the situations in which it emerges as a transformative *** acted as a catalyst for personal and collective empowerment,fostering new subjectivities while transcending physical and social *** study sparks multidimensional discourse on how technological tools can co-create our societies and identities,offering insights into how bicycles can drive socio-political change,promote emancipation and foster inclusivity.

关键词： bicycle female involvement in World WarⅡresistance movements cyborg theory

Changes in the adult sex ratio of six duck species breeding populations over two decades

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Avian Research 2024年第2期15卷 202-210页

作者： Monika Homolkova Petr Musil Diego Pavon-Jordan Dorota Gajdosova Zuzana Musilova sarka neuzilova Jan Zouhar Department of Ecology Faculty of Environmental SciencesCzech University of Life Sciences PragueKamýcka 129CZ-16521Prague–SuchdolCzech Republic Department of Terrestrial Ecology Norwegian Institute for Nature Research(NINA)PO Box 5685 TorgardenTrondheimN-7485Norway Department of Econometrics Faculty of Informatics and StatisticsUniversity of Economicsnam.W.Churchilla 4CZ-13067Prague 3Czech Republic

Despite all efforts,long-term changes in the adult sex ratios of breeding duck populations are still unclear;this uncertainty is especially true for male-bias populations,which are often under the scrutiny of researchers lacking convenient results for the active protection of endangered *** with male-bias populations are usually strongly affected by a decline in population size that leads to a higher extinction *** this study,we examined our long-term data of the abundance of breeding populations in six duck species(Mallard Anas platyrhynchos,Gadwall Mareca strepera,Red-crested Pochard netta rufina,Common Pochard Aythya ferina,Tufted Duck Aythya fuligula,and Common Goldeneye Bucephala clangula)from fishponds in south Bohemia,Czechia,between 2004 and *** evidence was used to assess long-term changes in the adult sex ratio in these breeding populations and investigate the possible effects of the nAO index(north Atlantic Oscillation index)on them,indicating climate conditions in *** determined a long-term decrease of the proportion of females in the breeding season in two of the six examined species:Common Pochard and Red-crested Pochard,which is driven by the long-term increase in the number of males in contrast to the decreasing or stable number of females likely caused by different migration behaviours between females and *** the case of Common Pochard,in breeding populations,we estimated 60-65%of males in the early 2000s rising to 75-80%in the early ***,we establish no significant effects linked to climate conditions of the previous winter in these species as a crucial cause of the changes of the proportion of females in the breeding population.

关键词： Adult sex ratio Breeding population Population dynamics Red-listed species Waterfowl Winter weather conditions

sc元素添加对双辊铸轧Al−Cu−Li−Mg−Zr基合金热处理的影响

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Transactions of nonferrous Metals society of China 2024年第6期34卷 1759-1773页

作者： Rostislav KRÁLÍK Barbora KŘIVsKÁ Lucia BAJTOŠovÁ Mykhailo sTOLBCHEnKO Mirko sCHAPER Olexandr GRYDIn Miroslav CIEsLAR Department of Physics of Materials Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116PragueCzech Republic Department of Materials science Faculty of Mechanical EngineeringUniversity of PaderbornWarburger Straße 10033098PaderbornGermany

提出制备含sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的sc,另一种未添加sc。两种合金... 详细信息

提出制备含sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的sc,另一种未添加sc。两种合金均先在300℃下退火30 min,然后在450℃下退火30 min以及530℃下退火30 min,随后进行室温水淬。前两段退火后组织中生成了细小、弥散的Al_(3)Zr/Al_(3)(sc,Zr)颗粒。最后的退火步骤起到了均匀化/固溶处理的作用。双辊铸轧材料的细小晶粒结构使含有主要合金元素的原始颗粒在较短的退火时间内溶解。缩短保温时间可以限制表面层中Li的耗尽和Al_(3)Zr/Al_(3)(sc,Zr)弥散相的明显粗化。铸态含sc材料已经具有明显细小的晶粒结构,而经均匀化/固溶处理后生成的Al_(3)(sc,Zr)弥散相抑制了晶粒在高温下发生粗化。在后续180℃下时效处理后,形成了θʹ(Al_(2)Cu)、T1(Al_(2)CuLi)和s'(Al_(2)CuMg)强化相。在峰值时效状态下,屈服强度提高了200 MPa,这一数值与类似直接冷却铸造材料的相当,从而证实了所提方案的合理性。

关键词：显微组织力学性能 Al−Cu−Li基合金 sc合金化双辊铸轧

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Deep-water cirripedes colonizing dead shells of the cephalopod nautilus macromphalus from new Caledonian waters

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Integrative Zoology 2019年第6期14卷 561-575页

作者： John BUCKERIDGE TomášKOČÍ Ján sCHLÖGL Adam TOMAŠovÝCH Martina KOČovÁVEsELsKÁ Earth&Oceanic systems Group RMIT UniversityMelbourneVictoriaAustralia Ivančická581 PragueCzech Republic and Palaeontological DepartmentNatural History MuseumNational MuseumPragueCzech Republic Department of Geology and Paleontology Comenius University in BratislavaBratislavaSlovakia Institute of Earth sciences Slovak Academy of SciencesBratislavaSlovakia Department of Paleobiology and Paleoecology Institute of Geology of the Czech Academy of SciencesPragueCzech Republic Museums Victoria MelbourneVictoriaAustralia Faculty of science Charles University in PraguePragueCzech Republic

Fossil cephalopods are frequently encrusted by epibionts;however,determining whether encrustation occurred prior to or post-mortem to the host,and whether the final environment of deposition corresponds to the habitat of encrustation is *** present paper describes cirripede epibionts,their calcareous bases and their attachment scars on 6 post-mortem shells of nautilus macromphalus,collected from deep water off new Cale*** cirripedes have left both cemented calcareous bases of Hexelasma and scars associated with bioerosion and discoloration produced by verrucomorph *** cirripedes included a Metaverruca recta,with articulated opercular plates and organic tissue(on a shell that had been exposed on the sea floor for at least 150 years),and specimens of Hexelasma velutinum,one of which was partly attached to an internal surface of a *** disposition of verrucomorphs indicates that most nautilus shells were colonized post-mortem rather than during a floating ***,as cirripedes are known to have colonized living nautilus,some Hexelasma,preserved only as calcareous eroded bases,may represent specimens that settled on a living *** degree of bioerosion and discoloration induced by verrucomorph barnacles varies according to the surface preservation of nautilus shells,with deeper and discolored traces preserved on old and degraded *** made by verrucomorphs described here are ellipsoidal and a new ichnotaxon,Anellusichnus ellipticus,is proposed to accommodate ***,verrucomorphs and other cirripede taxa with membranous bases that were attached to pristine shells may not leave any substantial scars,and,thus,will be difficult to detect in the fossil record.

关键词： Anellusichnus ellipticus isp.nov cirripedes epibionts Hexelasma velutinum Metaverruca recta

neuroprotective agents effective against radiation damage of central nervous system

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neural Regeneration Research 2022年第9期17卷 1885-1892页

作者： Mária Lalkovičová Laboratory of Radiation Biology Joint Institute for Nuclear ResearchDubnaRussia slovak Academy of sciences Institute of Experimental PhysicsKošiceSlovakia

Ionizing radiation caused by medical treatments,nuclear events or even space flights can irreversibly damage structure and function of brain *** can result in serious brain damage,with memory and behavior disorders,or even fatal oncologic or neurodegenerative *** used treatments and drugs are mostly targeting biochemical processes of cell apoptosis,radiation toxicity,neuroinflammation,and conditions such as cognitive-behavioral disturbances or others that result from the radiation *** most drugs,the side effects and potential toxicity are also to be ***,many agents have not been approved for clinical use *** this review,we focus on the latest and most effective agents that have been used in animal and also in the human research,and clinical *** could have the potential therapeutical use in cases of radiation damage of central nervous system,and also in prevention considering their radioprotecting effect of nervous tissue.

关键词： antioxidants biomedical neuroprotectants central nervous system ionizing radiation neuroprotection radiation injury radiomitigators radioprotectants radioprotection therapeutics

Advantageous mechanochemical synthesis of copper(Ⅰ)selenide semiconductor,characterization,and properties

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Frontiers of Chemical science and Engineering 2022年第3期16卷 433-442页

作者： Katarína Gáborová Marcela Achimovičová Michal Hegedüs Vladimír Girman Mária Kaňuchová Erika Dutková Institute of Geotechnics Slovak Academy of SciencesKošice04001Slovakia Institute of Metallurgy Faculty of MaterialsMetallurgy and RecyclingTechnical UniversityKošice04201Slovakia synthon s.r.o.Blansko67801Czech Republic Faculty of science Pavol JozefŠafárik UniversityKošice04154Slovakia Institute of Earth Resources Faculty of MiningEcologyProcess Control and GeotechnologiesTechnical UniversityKošice04200Slovakia

Copper(I)selenide-nanocrystalline semiconductor was synthesized via one-step mechanochemical synthesis after 5 min milling in a planetary ball *** kinetics of synthesis was followed by X-ray powder diffraction analysis and specific surface area measurements of milled 2Cu/se *** X-ray diffraction confirmed the orthorhombic crystal structure of Cu_(2)se with the crystallite size∼25 *** surface chemical structure was studied by X-ray photoelectron spectroscopy,whereby the binding energy of the Cu 2p and se 3d signals corresponded to Cu^(+)and se^(2−)oxidation *** electron microscopy revealed agglomerated nanocrystals and confirmed their orthorhombic structure,as *** optical properties were studied utilizing ultraviolet-visible spectroscopy and photoluminescence *** direct bandgap energy 3.7 eV indicated a blue-shift phenomenon due to the quantum size *** type of Cu_(2)se synthesis can be easily adapted to production dimensions using an industrial vibratory *** advantages of mechanochemical synthesis represent the potential for inexpensive,environmentally-friendly,and waste-free manufacturing of Cu_(2)se.

关键词： Cu_(2)se berzelianite nanocrystalline semiconductor mechanochemical synthesis planetary ball mill

Predicting the output error of the suboptimal state estimator to improve the performance of the MPC-based artificial pancreas

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Control Theory and Technology 2023年第4期21卷 541-554页

作者： Martin Dodek Eva Miklovičová Institute of Robotics and Cybernetics Faculty of Electrical Engineering and Information TechnologySlovak University of Technology in BratislavaIlkovičova 3Bratislava81219Slovakia

The error of single step-ahead output prediction is the information traditionally used to correct the state estimate while exploiting the new measurement of the system ***,its dynamics and statistical properties can be further studied and exploited in other *** is known that in the case of suboptimal state estimation,this output prediction error forms a correlated sequence,hence it can be effectively predicted in real *** a suboptimal scenario is typical in applications where the process noise model is not known or it is ***,the paper deals with the problems of analytical and empirical modeling,identification,and prediction of the output error of the suboptimal state estimator for the sake of improving the output prediction accuracy and ultimately the performance of the model predictive *** improvements are validated on an empirical model of type 1 diabetes within an in-silico experiment focused on glycemia prediction and implementation of the MPC-based artificial pancreas.

关键词： suboptimal state estimation Prediction Autoregressive model Moving average model Model predictive control Diabetes mellitus Artificial pancreas

super d-antimagic Labelings of Disconnected Plane Graphs

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Acta Mathematica sinica,English series 2010年第12期26卷 2283-2294页

作者： Martin BAA Mirka MILLER Oudone PHAnALAsY Andrea sEMAnIov-FEovKov Department of Applied Mathematics Technical University school of Electrical Engineering and Computer science The University of Newcastle Department of Mathematics University of West Bohemia Department of Computer science King's College London Department of Mathematics National University of Laos

This paper deals with the problem of labeling the vertices, edges and faces of a plane graph in such a way that the label of a face and the labels of the vertices and edges surrounding that face add up to a weight of that face, and the weights of all s-sided faces constitute an arithmetic progression of difference d, for each s that appears in the graph. The paper examines the existence of such labelings for disjoint union of plane graphs.

关键词： Plane graph d-antimagic labeling super d-antimagic labeling disjoint union of graphs