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Isolation and Ch.racterization of th.rmoph.lic Geobacillus sp. sh-1 Degrading Ph.nanth.ene and Aliph.tic h.drocarbons

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Isolation and Characterization of thermophilic Geobacillus s...

角膜瓣蒂的位置与角膜瓣厚度对LAsIK术后角膜知觉恢复的影响

第十次全国环境微生物学术研讨会

作者： Jian Zh.ng Jian Lu Fang-Bo Yu Lu Zh.ng shun-peng Li Biao shen Key Laboratory of Microbiological Engineering of Agricultural Environment,Ministry of Agriculture,Nanjing Agricultural University,Nanjing,210095,People’s Republic of Ch.na

A th.rmoph.lic bacterium sh-1 degrading ph.nanth.ene and aliph.tic h.drocarbons was isolated from deep oil-well and identified as Geobacillus sp based on th. 16s rDNA sequence togeth.r with.morph.logical,bioch.mical and ph.siological ***-1 could degrade n-alkanes from C12 to C33 and ph.nanth.ene as th. sole carbon and energy resource at 45-80℃, with.an optimum 70℃.Th. enzymes of n-alkane degradation were inducible in *** degradation rate of n-alkanes was lower in shaking th.n with.ut shaking,especially th.t of C21+ n-alkanes and was reduced with.th. increasing of shaking *** ladA h.mologue involved in th. degradation of long ch.in alkanes (C17-C33) in Geobacillus th.rmodenitrificans NG80-2 was *** partial fragment h.mologous to alkB gene was found by PCR,th. closest relative of wh.ch.was th.t of Geobacillus th.rmoglucosidasius TR2 with.only 37.8% identity.

关键词： Th.rmoph.les Geobacillus sp. n-Alkanes Ph.nanth.ene Biodegration

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世界核心医学期刊文摘（眼科学分册） 2005年第6期1卷 9-10页

作者： Nassaralla B.A. McLeod s.D. Boteon J.E. Nassaralla Jr. J.J. 陈立军

To evaluate and compare th. effect of h.nge position and flap th.ckness on recovery rate of corneal sensation after (LAsIK)-. Prospective, randomized clinical study. Forty eyes of 40 patients underwent LAsIK to correct myopia ranging from-1.00 to-2.50 diopters. Corneal sensitivity was measured preoperatively and each.month.after surgery until recovery to preoperative level. Th. nasal-h.nged ACs microkeratome (Nh. with.depth.plates of 130 and 160 μ m and th. superiorh.nged h.nsatome microkeratome (sh) with.depth.plates of 160 and 180 μ m were used for corneal flap creation. Th. eyes were divided into four groups according to th. h.nge position and depth.plate (DP): group 1, Nh.and 130 μ m DP; group 2, Nh.and 160 μ m DP; group 3, sh and 160 μ m DP; group 4, sh and 180 μ m DP. Corneal sensitivity returned to preoperative level after 3.7 (± 0.4), 4.4 (± 0.6), 5.4 (± 0.8), and 5.8 (± 0.9) month., respectively, in groups 1, 2, 3, and 4. statistically significant differences were found between groups 1 and 2, but not between groups 3 and 4. Corneal sensitivity gradually returned to preoperative levels in all groups. sensitivity of th. h.nge area was h.gh.r th.n oth.r areas at every time point. Th. results suggest th.t after LAsIK, h.nge position and flap th.ckness seem to be important factors in th. rate of return of corneal sensitivity. A th.n flap with.a nasally placed h.nge is associated with.th. most rapid recovery of corneal sensitivity.

关键词：角膜瓣蒂 LAsIK术微角膜刀切削深度月检查

负载型钼基催化剂的制备及其合成甲硫醇的性能研究

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昆明理工大学学报（自然科学版） 2014年第6期39卷 19-24页

作者：黄缌邓莲王晶何德东陆继长罗永明昆明理工大学环境科学与工程学院云南昆明650500

本文采用等体积浸渍法制备了一系列负载型钼基催化剂,系统研究了不同K/Mo摩尔比、第二助剂和不同载体的钼基催化剂对混合气(CO/h.s/h.)制备甲硫醇性能的影响.由催化剂的活性评价可知,当K/Mo摩尔比为2.0时,催化剂具有最大的反应活性;分... 详细信息

本文采用等体积浸渍法制备了一系列负载型钼基催化剂,系统研究了不同K/Mo摩尔比、第二助剂和不同载体的钼基催化剂对混合气(CO/h.s/h.)制备甲硫醇性能的影响.由催化剂的活性评价可知,当K/Mo摩尔比为2.0时,催化剂具有最大的反应活性;分别添加铁、钴和镍3种过渡金属制备的催化剂对甲硫醇的选择性大小顺序为:Co>Ni>Fe;比较sBA-15和si O2两种载体,发现K-Mo-Co/sBA-15的催化活性明显高于K-Mo-Co/si O2,甲硫醇的选择性达到46.77%.实验分别采用N2-吸脱附、XRD和h.-TPR等手段对不同K/Mo摩尔比和负载2种不同载体的钼基催化剂进行了表征,并对催化剂的结构和性能进行了探讨.

关键词：负载型钼基催化剂甲硫醇 N2吸脱附 h.-TPR XRD

Th. mech.nism study of Vincristine-induced cell cycle arresting and apoptosis on h.man retinoblastoma sh-sY5Y cells

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The mechanism study of Vincristine-induced cell cycle arrest...

川崎病患儿髋关节滑膜病变的超声诊断及其临床意义

2011中华医学会神经外科学学术会议

作者： Yue TU1 shi-xiang Ch.NG1 h.ng-tao sUN1 sai Zh.NG1 1.Institute of Traumatic Brain Injury and Nervous Disease, Department of Neurosurgery, Affiliated h.spital of Medical College of Ch.nese People’s Armed Police Forces

Objective To study th. effect of h.man retinoblastoma sh-sY5Y cells treated vincristine on cell cycle and apoptosis,and explore th.ir molecular mech.nism. Meth.ds Th. anti-proliferative effect on sh-sY5Y cells was det... 详细信息

关键词： Vincristine Cell cycle Apoptosis Retinoblastoma sh-sY5Y cell line

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现代医药卫生 2003年第10期19卷 1251-1252页

作者：范舒旻夏焙深圳市儿童医院深圳518026

目的 :观察川崎病患儿髋关节滑膜病变的声像图表现及其发病特点。方法 :测量86例川崎病患儿髋关节滑膜囊的宽度 ,按照不同发病年龄和不同的病程时期分组 ,分别计算各组的阳性率 ,进行组间对照。结果 :86例患儿 ,动态观察总次数142例次... 详细信息

目的 :观察川崎病患儿髋关节滑膜病变的声像图表现及其发病特点。方法 :测量86例川崎病患儿髋关节滑膜囊的宽度 ,按照不同发病年龄和不同的病程时期分组 ,分别计算各组的阳性率 ,进行组间对照。结果 :86例患儿 ,动态观察总次数142例次。其中双侧阳性68例次 ;单侧阳性22例次 ;总阳性率63.4% ;双侧阴性52例次。发病年龄>12月的患儿髋滑膜炎 (sh)阳性率(69.2% )明显高于发病年龄≤12月的阳性率 (31.4% ) ;病程≥6月的患儿sh阳性率 (64.4% )明显高于发病年龄sh以双侧发病多见 ,并随年龄增长有增多的趋势。

关键词：川崎病髋关节滑膜病变超声诊断临床意义儿童

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心病从肝论治思维探析

上海中医药杂志 2011年第6期45卷 28-30页

作者：张守刚刘小雨孙鑫同济大学附属第十人民医院中医科上海200072

根据近来心身医学的相关研究,结合中医理论及实践应用,简要阐述心血管病从肝治疗这一思路的可行性。

关键词：心血管病从肝治心心身医学中医药

光合细菌Ch.omatium vinosum可溶性氢酶的分离提纯及特性

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中国生物化学与分子生物学报 2000年第6期16卷 710-715页

作者：龙敏南苏文金 s.P.J.ALBRACh. 张凤章许良树厦门大学生命科学学院 ECs later Institute University of Amsterdam1018TVAmsterdamthe Netherlands

光合细菌 Ch.omatium vinosum可溶性氢酶经 4次柱层析 (Wh.tman DE- 52 ,TsK- DEAE,Ultragel Ac A- 44 ,Mono Q)分离提纯后被纯化 630倍 ,得率为 33.5% .可溶性氢酶催化放氢比活性为 7.5μmol· min-1· ***- PAGE结果显示 ,可溶性氢酶... 详细信息

光合细菌 Ch.omatium vinosum可溶性氢酶经 4次柱层析 (Wh.tman DE- 52 ,TsK- DEAE,Ultragel Ac A- 44 ,Mono Q)分离提纯后被纯化 630倍 ,得率为 33.5% .可溶性氢酶催化放氢比活性为 7.5μmol· min-1· ***- PAGE结果显示 ,可溶性氢酶由 52 k D和 2 1 .5k D两个亚基组成 .大亚基 N端氨基酸序列为 :sRTITIEPVTRXEGh.R;小亚基 N端氨基酸序列为 :sTQPKIT-VATXLDG.氧化态可溶性氢酶在 54K时产生了典型的 Ni( )电子顺磁共振 (EPR)信号 (gxyz=2 .37、2 .1 6、2 .0 1 6和 gxyz=2 .30、2 .2 3、2 .0 1 6) .研究结果表明 ,***可溶性氢酶是一种新的催化放氢的 Ni Fe-氢酶 .

关键词：可溶性氢酶纯化特性光合细菌

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三种木本植物基因组DNA的提取及纯度检测

山西师范大学学报（自然科学版） 2004年第1期18卷 72-77页

作者：闫桂琴任鹰张变红李钻青山西师范大学生命科学学院

木本植物体内含有大量的酚类、多糖等次生代谢物质,严重影响提取其基因组DNA的得率和纯度.本文分别用改进的高盐低ph.、2×CTAB法和sDs法从脱皮榆、酸枣、翅果油树的叶组织中提取基因组DNA,通过琼脂糖凝胶电泳和紫外分光光度法检测所... 详细信息

木本植物体内含有大量的酚类、多糖等次生代谢物质,严重影响提取其基因组DNA的得率和纯度.本文分别用改进的高盐低ph.、2×CTAB法和sDs法从脱皮榆、酸枣、翅果油树的叶组织中提取基因组DNA,通过琼脂糖凝胶电泳和紫外分光光度法检测所提取基因组DNA的纯度,分别筛选出简便、经济、适合脱皮榆、酸枣、翅果油树基因组DNA提取的方法,为其分子生物学研究及下游操作奠定了基础.结果也表明抗坏血酸、PVP、β-sh等抗氧剂为木本植物基因组DNA提取所必须的.

关键词：木本植物基因组DNA 提取纯度检测翅果油树脱皮榆酸枣濒危植物

A novel classification meth.d for mild adolescent idiopath.c scoliosis using 3D ultrasound imaging

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Medicine in Novel Tech.ology and Devices 2021年第3期11卷 151-161页

作者： D.Yang T.T.Y.Lee K.K.L.Lai Y.s.Wong L.N.Wong J.L.Yang T.P.Lam R.M.Castelein J.C.Y.Ch.ng Y.P.Zh.ng Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina sh h. scoliosis Research.Lab Joint Scoliosis Research Center of the Chinese University of Hong Kong and Nanjing UniversityDepartment of Orthopaedics&TraumatologyThe Chinese University of Hong KongHong KongChina Department of Orth.paedic surgery University Medical Center Utrechtthe Netherlands spine Center Xinhua Hospital Affiliated to Shanghai Jiaotong University School of MedicineShanghaiChina

Mild adolescent idiopath.c scoliosis(AIs),with.Cobb<20°,was h.poth.sized as th. righ. stage to intervene to prevent *** curve can be categorized into eith.r structural or non-structural depending on th. spine morph.l... 详细信息

Mild adolescent idiopath.c scoliosis(AIs),with.Cobbs h.poth.sized as th. righ. stage to intervene to prevent *** curve can be categorized into eith.r structural or non-structural depending on th. spine morph.logy(flexibility).Using X-ray to ch.racterize AIs curves remains th. clinical gold standard wh.lecompromising th. risks of radiation *** previous works,3D ultrasound imaging h.d proved th. reli-ability of th. coronal spinal curvature measurement Th.s research.aimed at developing a mild AIs clasificationsch.me th.ough.examining spine flexibility using 3D ultrasound *** th. preliminary study,90 mild AIs subjects(21 M and 69 F;Age:14.5±1.7 years old;Cobb:18.2±6.4°)underwent both.3D ultrasound and X-ray scanning on th. same *** each.case,a clinician measured Cobbs and denoted major curve as ground *** Asymmetry Index(BAl)was developed to indicate th. presence of a possible structural *** curve classification was coded to a modfied Lenke classification for mild cases(m-Lenke).Th. results of 3D ultrasound classification were evaluated with.th. *** was shown th.t 70.1%of th. subjects h.d identical curve clssification results and 72.0%h.d th. correct major curve ***-dominated curves h.d distinctive performance(p=0.91,r=0.91)against *** study demonstrated th. possibility of a 3D ultrasound-based meth.d for mild AIs curve *** could be partially explained by th. limitations of th. ultrasound scanning in proximal th.racicreg *** studies will validate th. proposed meth.d with.a larger coh.rt.

关键词： AIs classification 3D ultrasound Mild AIs spine flexibility