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心肌淀粉样变性的心肌细胞外容积定量：心脏CT与MRI的比较研究

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国际医学放射学杂志 2024年第2期 253-253页

作者： H.Hayashi s.Oda M.Kidoh s.Yamaguchi F.Yoshimura s.takashio 朱婉秋

摘要目的 CT显示心肌细胞外容积（ECV）是心脏MR(CMR)的替代方法，具有显著的临床应用优势。然而，在心肌淀粉样变性（CA）中分别通过CT和CMR测量ECV的一致

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Bioethanol Production from Molasses by Yeasts with stress-Tolerance Isolated from Aquatic Environments in Japan

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Advances in Microbiology 2019年第12期9卷 1000-1011页

作者： Yuka Naito Masahiko Okai Masami Ishida Masachika takashio Naoto Urano Laboratory of Marine Biochemistry Department of Ocean Sciences Tokyo University of Marine Science and Technology Konan Minato-ku Tokyo Japan Zensho Laboratories of Food Technology Zensho Holdings Co. Ltd. Konan Minato-ku Tokyo Japan

Bioethanol is a safe and renewable source of energy that continues to be a research focus, since fossil fuels have been linked to global warming and nuclear energy sources are affected by the increased safety concerns following the 2011 nuclear power plant accident in Japan. In general, bioethanol is converted from a biomass by yeast fermentation. The production efficiency of this bioethanol is not sufficiently high, and its practical use as a substitute for fossil fuels and nuclear energy is thus limited. For the industrial production of bioethanol, the yeast fermentation of biomass cultures containing high concentration sugar, NaCl, and ethanol is necessary, but this might induce phenomena in which the stresses arising in the yeasts weaken their cells during fermentation. As described herein, we isolated 1028 strains of yeasts from natural aquatic environments: Japan’s Tama River and Lake Kasumigaura. Among them, 412 strains were fermentative yeasts and 31 strains showed high fermentation ability under a 30% sorbitol + 10% ethanol condition. These strains were identified as Torulaspola delbrueckii, Wickerhamomyces anomalus, Candida glabrata, Pichia kudriavzevii, saccharomyces cf. cerevisiae/paradoxus, and Lachancea kluyveri. The strains T. delbrueckii, W. anomalus, and C. glabrata also showed tolerance against 15% NaCl. Most importantly, s. cf. cerevisiae/paradoxus H28 and L. kluyveri F2-67 produced 57.4 g/L and 53.9 g/L ethanol from molasses (sucrose 104.0 g/L, fructose 33.4 g/L, and glucose 24.8 g/L) within 48 hrs at 25°C, respectively.

关键词： stress-Tolerance Molasses Yeast species Aquatic Environment Fermentation

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Molecular Phylogeny and Phenotypic Characterization of Yeasts with a Broad Range of pH Tolerance Isolated from Natural Aquatic Environments

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Advances in Microbiology 2019年第1期9卷 56-73页

作者： Naoto Urano Ayaka shirao Yuka Naito Masahiko Okai Masami Ishida Masachika takashio Department of Ocean sciences Laboratory of Marine Biochemistry Tokyo University of Marine Science and Technology Tokyo Japan Zensho Laboratories of Food Technology Zensho Holdings Co. Ltd. Tokyo Japan

In this study, yeasts with broad range of pH tolerance were isolated and characterized from natural aquatic environments in Japan. Only a few basic and application studies of alkali-tolerant yeasts have been reported, despite the unmet industrial needs. First, we surveyed alkali-tolerant yeasts from natural aquatic environments at pH 7.6 - 9.4. We isolated 35 yeast strains that grew in pH 9.0 medium, from seven genera and nine species: 25 strains (N1, N2, through N6, N9, K1, K3 through K19) were Rhodotorula mucilaginosa;one (N7) was Rhodosporidium fluvial;one (N8) was scheffersomyces spartinae;two (N10 and N13) were Wicherhamonyces anomalus;one (N11) was Cyberlindnera saturnus;one (s1) was Candida sp.;two (s2 and s4) were Candida intermedia;one (s3) was Candida quercuum;and one (K2) was Cryptococcus liquefacience. We examined the effects of pH on the growth of representative yeast strains. strains K12 and s4 showed high growth at pH 3 - 10. strains N7, N8, N10, N11, and s3 showed high growth at pH 3 - 9. strains K2 and s1 showed high growth at pH 4 - 8. All nine of these strains had neutralizing activities from acidic media at pH 3 - 5 to pH 6 - 8. We previously isolated acid-tolerant yeasts (Cryptococcus sp. T1 [1] and Candida intermedia CeA16 [2]) from extremely acidified environments;they showed high growth at pH 3 - 9 and neutralizing activities of acidic media by releasing ammonium ions. Thus, alkali-tolerant yeasts and acid-tolerant yeasts were found to be similar species and have both high growth at a broad range of pH and neutralizing activities of acid media. Previously, we also isolated acid-tolerant, acid-neutralizing yeasts from neutral natural environments (26 strains, 12 species) [3]. Next, we constructed the phylogenetic trees of both acid-tolerant yeasts and alkali-tolerant yeasts. All were situated in the same classification position. similar yeast species with a broad range of pH tolerance were living in natural aquatic environments at pH values fr

关键词： pH Tolerance Yeast species Aquatic Environment Phylogenetic Tree Fermentation

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Ethanol Fermentation by High-stress-Tolerance Aquatic Yeasts and Their Mutants

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Advances in Microbiology 2021年第11期11卷 616-629页

作者： Naoto Urano Masami Ishida Yuka Naito Rintaro Endo Toshinori Takei Masachika takashio Masahiko Okai Laboratory of Marine Biochemistry Department of Ocean Sciences Tokyo University of Marine Science and Technology Tokyo Japan Zensho Laboratories of Food Technology Zensho Holdings Co. Ltd. Tokyo Japan

Bioethanol is thought to be a renewable source of energy, because the biomasses used to make ethanol, such as sugar cane and its residual substance, molasses, are resources that can be continuously produced. But the practical use of ethanol to replace fossil fuels or atomic energy has been limited, because the production efficiencies of ethanol in relation to its substrates are not so high. Thus, for industrial production of the bioethanol, yeast fermentation would ideally be carried out in biomasses containing more highly concentrated carbohydrates. However, the environmental stresses in highly concentrated cultures might weaken the yeast’s physiological activities. From various kinds of aquatic yeast with stress tolerance, Torulaspora derbrueckii F2-11 and Wicherhamomyces anomalus AN2-64 were selected as candidates for high-sugar-tolerance yeasts as they showed remarkable growth in the YPD + sorbitol (600 g/L) medium at 25°C for 120 hrs. When the amounts and kinds of sugar alcohols in the cells of the two strains were measured in cultures containing 20 g/L or 400 g/L of D-glucose, maltose, or sucrose, the main two sugar alcohols that accumulated as the sugar concentration increased were glycerol and arabitol. Mutation by ethyl methanesulfonate of the parent strains T. derbrueckii F2-11 and W. anomalus AN2-64 induced mutants F2-11M or AN2-64M, which showed higher sugar, heat, and ethanol tolerances than their respective parents. Ethanol productivities and sugar assimilation activities of the mutants were also higher than those of the parents in the 25% (v/v) molasses.

关键词： Ethanol Fermentation stress Tolerance Aquatic Yeast Mutant Ethyl

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H2FPEF评分预测左心室射血分数保留的心力衰竭患者预后的价值

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中华高血压杂志(中英文) 2020年第6期 598-598页

作者： sueta D Yamamoto E Nishihara T Tokitsu Takanori Fujisue Koichiro Oike Fumi Takae M Usuku H takashio s Arima Y suzuki s Nakamura T Ito M Kanazawa H sakamoto K Kaikita K Tsujita Kenichi 周卫

H2FPEF[H2表示体质量增加（heavy）和高血压（hypertensive）、F表示心房颤动（atrial fibrillation）、P表示肺动脉高压（pulmonary hypertension）、E表示老年（elder）、F表示充盈压（filling pressure）]评分是诊断左心室射血分数...

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左心室射血分数保留的心力衰竭患者的血钾水平和心血管事件

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中华高血压杂志(中英文) 2019年第8期 798-798页

作者： Nishihara T Tokitsu T sueta D Takae M Oike F Fujisue K Usuku H takashio s Hanatani s Kanazawa H Arima Y sakamoto K Izumiya Y Yamabe H Kaikita K Yamamoto E Tsujita K 陈云叶鹏

虽然血钾水平与慢性心力衰竭患者预后密切相关,但血钾水平在射血分数保留的心力衰竭(heart failure with preserved ejection fraction,HFpEF)患者的心血管结局中的临床意义尚不完全清楚。方法:本研究是回顾性单中心的观察研究,共招募... 详细信息

虽然血钾水平与慢性心力衰竭患者预后密切相关,但血钾水平在射血分数保留的心力衰竭(heart failure with preserved ejection fraction,HFpEF)患者的心血管结局中的临床意义尚不完全清楚。方法:本研究是回顾性单中心的观察研究,共招募入住熊本大学医院的持续性HFpEF患者506例,并根据出院时血钾水平的四分位数分为4组(组1:血钾<4.1,组2:血钾4.1～<4.4,组3:血钾4.4～<4.7,组4:血钾

关键词：射血分数保留的心力衰竭心血管事件钾水平左心室

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