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通过星形胶质细胞转录组分析寻找脑卒中治疗靶标

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神经损伤与功能重建 2019年第2期14卷 109-109页

作者： Rakers C schleif M Blank n Matu?ková H Ulas T H?ndler K Torres sV schumacher T Tai K schultze JL Jackson Ws Petzold GC 聂昊 German Center for neurodegenerative Diseases (DZnE) Department of neurology University Hospital Bonn Genomics and Immunoregulation LIMES-Institute University of Bonn

星形胶质细胞支持正常的脑功能,但当它们在卒中等病理条件下变得具有反应性时也可能促成神经退行性病变。然而,反应性星形胶质细胞增生的有益和有害特性之间依赖于背景的相互作用的分子基础仍然未被完全理解。我们使用RiboTag技术,在短... 详细信息

星形胶质细胞支持正常的脑功能,但当它们在卒中等病理条件下变得具有反应性时也可能促成神经退行性病变。然而,反应性星形胶质细胞增生的有益和有害特性之间依赖于背景的相互作用的分子基础仍然未被完全理解。我们使用RiboTag技术,在短暂性大脑中动脉闭塞(tMCAO)72 h后的小鼠星形胶质细胞中特异性地纯化翻译m RnA,从而产生卒中特异性星形胶质细胞翻译组数据库。与对照组大脑相比,t MCAO后的反应性星形胶质细胞显示与A2表型相关的转录物富集,这与神经保护相关。我们还发现,星形胶质细胞也会上调大量潜在的神经毒性基因。本研究共鉴定了1,003个基因和38个转录因子的差异表达,其中stat3,sp1和spi1是最显著的。为了进一步探索stat3介导的途径对卒中发病机制的影响,我们对星形胶质细胞特异性条件性缺失stat3的小鼠进行tMCAO处理,发现局灶性缺血72 h后的小鼠脑梗死体积减小,运动功能改善。综上所述,本研究扩展了新兴的星形胶质细胞特异性靶向卒中治疗数据库,支持星形胶质细胞在生理和病理条件下均对脑功能提供了关键保障作用的观点。

关键词：星形胶质细胞缺血下一代测序

捷克LGMD2A患者的组织病理学和分子病理学分析结果

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世界核心医学期刊文摘（神经病学分册） 2006年第6期2卷 52-52页

作者： Hermanová M. Zapletalová E. sed l áková J. 牛亚利 Department of Pathology University Hospital Brno Jihlavská20 625 00 Brno Czech Republic Dr.

Limb-girdle muscular dystrophy type 2A (LGMD2A) is an autosomal-recessive disorder characterized by selective atrophy and progressive weakness of proximal girdle muscles. LGMD2A, the most prevalent form of LGMD, is caused by mutations in the CAPn3 gene that encodes the skeletal musclespecific member of the calpain family, calpain-3 (p94). We examined the histopathologic and molecular pathologic findings in 14 Czech LGMD2A patients. Analysis of the CAPn3 gene was performed at the mRnA level, using reverse transcription-polymerase chain reaction (RT-PCR) and sequencing, and/or DnA level, using PCR and denaturing high-performance liquid chromatography (DHPLC). Our results confirm that mutation 550delA is the most frequent CAPn3 defect in Czech LGMD2A patients (9 alleles of 28). Furthermore, we established that, in a patient with the 550delA/R490W genotype, mRnA carrying frameshift mutation 550delA was not detected, probably due to its degradation by nonsense-mediated mRnA decay. In muscle biopsies of two LGMD2A patients, a neurogenic pattern simulating a neurogenic lesion was observed. Immunoblot analysis revealed the deficiency of p94 in all genetically confirmed cases of LGMD2A, and secondary dysferlin deficiency was demonstrated on muscle membranes in 6 patients using immunofluorescence. Thus, we find a combination of DnA and mR nA mutational analysis to be useful in the diagnosis of LGMD2A. Moreover, our study expands the spectrum of calpainopathies to cases that simulate a neurogenic lesion in muscle biopsies, and the knowledge of possible secondary deficiencies of muscular proteins also contributes to a diagnosis of LGMD2A.

关键词：患者实施分子病理学组织病理学分析结果捷克肢带型肌营养不良钙激活中性蛋白酶 mRnA水平变性高效液相色谱法逆转录聚合酶链反应

孕≤16周高危人群的葡萄糖耐量试验有利于预测和排除随后的妊娠期糖尿病

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世界核心医学期刊文摘（妇产科学分册） 2005年第11期1卷 14-14页

作者： Bitó T.nyári T. kovács L. Pál A. 高雪莲 szülészeti s n■gyógyászati Klinika Semmelweis u1 H-6725 Szeged Hungary

Background: An oral glucose tolerance test with a result that is negative but close to the diagnostic cut-off in early pregnancy was hypothesized to serve as a predictor of subsequent gestational diabetes in a high risk group. The aim of the study was to determine those cut-off values of OGTT at gestational weeks ≤ 16, which can predict or exclude subsequent onset of GDM in a high risk group. Methods: Pregnant women at high risk of gestational diabetes (n = 163) underwent a 2-h, 75-g oral glucose tolerance test at gestational weeks ≤ 16 were analyzed in this study. In the event of a negative result, subsequent oral glucose tolerance tests were performed at gestational weeks 24-28 and 32-34. The sensitivity, the specificity, the positive and negative predictive values and the Odds ratio of the best cut-off values of fasting and postload glucose levels were calculated. Results: The best cut-off values to exclude subsequent GDM for fasting and postload glucose were 5.0 and 6.2 mmol/l, respectively. In combination, the best cut-off values were 5.3 mmol/l for fasting and 6.8 mmol/l for postload glucose, with negative predictive values of 0.97 and 0.71 and sensitivities of 96.9 and 86.3 at gestational weeks 24-28 and 32-34, respectively. Combination of these cut-off values with obesity proved to be very predictive for gestational diabetes by gestational weeks 32-34, with an Odds ratio of 6.0 95% confidence interval: 1.7-21.0 . Conclusions: With regard to the very high negative predictive value of the method, pregnant women with glucose levels of ≤ 5.3 mmol/l at fasting and of ≤ 6.8 mmol/l at postload in gestational weeks ≤ 16 should undergo subsequent oral glucose tolerance testing merely at gestational weeks 3234. Approximately a quarter (24.5% ) of the pregnant women at risk of gestational diabetes satisfied these criteria.

关键词：妊娠期糖尿病葡萄糖耐量试验高危人群餐后血糖高危孕妇血糖值妊娠早期阴性预测值界值阳性预测值

无术前激素准备的经宫颈子宫内膜切除术的长期有效性

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世界核心医学期刊文摘（妇产科学分册） 2006年第10期2卷 19-19页

作者： Molnár B.G. Kormányos Z. kovács L. Pál A. 朱晓明 University of szeged Semmelweis u. 1 H-6725 Szeged Hungary

Objective: To assess the level of patient satisfaction after transcervical endometrial resection(TCRE) with no preoperative hormonal preparation. study design: A retrospective audit of a continuous case series was accomplished on 131 consecutive patients who underwent TCRE for dysfunctional uterine bleeding. Data of postal questionnaires were analysed and subjected to survival analysis. Results: Thirty-three cases were lost to follow-up; thus, the data on 98 of the 131(74.8%)-patients were analysed. The average follow-up period was 94.8 months(60-132). Twenty(20.4%) women required D&C and 15(15.3%) had hysterectomy. In eight of the 15 cases, the indication for hysterectomy was not related with the primary operation. The chance of avoiding hysterectomy reached a plateau after 72 months, at 78.3%(sE: 5.05%). The chance of avoiding D&C at up to 36 months was 98.6%(sE: 1.4%), and reached a plateau after 107 months at 67.11%(sE: 6.1%)-; 55.8%of the patients became amenorrhoeic, the remaining cases reporting good improvements in the amount and duration of bleeding, and dysmenorrhoea. Eighty-six of the 98 patients(88%) were satisfied or very satisfied with the result. Conclusions: TCRE affords reasonable long-term effectiveness in the treatment of dysfunctional uterine bleeding, even without any preoperative hormonal endometrial preparation.

关键词：子宫内膜切除术长期有效性生存分析失访问卷数据研究设计持续时间

主动脉根部尺寸主要由遗传因素所决定:经典孪生个体研究

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国际医学放射学杂志 2017年第4期 492-492页

作者： C.Celeng M.Kolossváry A.kovács A.á.Molnár B.szilveszter T.Horváth 吕岳华 MTA-sE Cardiovascular Imaging Research Group Heart and Vascular Center Semmelweis University

摘要目的既往研究报道使用经胸腔超声心动图(TTE)观察到主动脉根部尺寸具有中等遗传性。CT血管成像(CTA)可能会提供更准确的遗传性评价。本研究的主要目的是使用CTA评估主动脉根部的遗传性;次要目的是得到基于TTE的遗传性数据,并将其与...

摘要目的既往研究报道使用经胸腔超声心动图(TTE)观察到主动脉根部尺寸具有中等遗传性。CT血管成像(CTA)可能会提供更准确的遗传性评价。本研究的主要目的是使用CTA评估主动脉根部的遗传性;次要目的是得到基于TTE的遗传性数据,并将其与基于CTA的数据相比较。方法在布达佩斯全球研究(BUDAPEsT-GLOBAL)项目中,198对双胞胎[其中118对为单合子,80对为双合子,年龄为(56.1±

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C/EBPβ控制耐力运动诱发的心脏生理性生长和抗病理性重构

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中南医学科学杂志 2013年第5期41卷 519-519页

作者： Pontus Bostrm nina Mann Jun Wu Pablo A Quintero Eva R Plovie Daniela Panáková Rana K Gupta Chun-yang Xiao Calum A MacRae Anthony Rosenzweig Bruce M spiegelman

心肌能通过使其细胞增大来适应外界压力的增加，这一应激反应适应于生理性刺激或病理性刺激对心脏造成的高负荷压力。例如，在负荷运动，高血压等刺激下，心肌都会出现明显的肥大和增殖现象。病理性心肌肥大可导致心力衰竭或心律失常，... 详细信息

心肌能通过使其细胞增大来适应外界压力的增加，这一应激反应适应于生理性刺激或病理性刺激对心脏造成的高负荷压力。例如，在负荷运动，高血压等刺激下，心肌都会出现明显的肥大和增殖现象。病理性心肌肥大可导致心力衰竭或心律失常，但生理性肥厚却不能引起这些疾病，所以弄清楚这两种类型的心肌肥大分子机制具有重要的临床意义。

关键词：病理性重构生理性 C EBPβ 运动诱发心脏心肌肥大生长耐力

俄罗斯北极东部拉普捷夫海大陆架的构造及地质

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海洋地质 2017年第F12期33卷 104-127页

作者： sergei s.Drachev Leonid A.savostin Victor G.Groshev 周娇(翻译) 吴峧岐(校对) 万玲(校对) 范广慧(校对) 不详广州海洋地质调查局海洋区域地质调查所

拉普捷夫海对于研究大陆初始张裂的过程具有重要意义。它位于加克尔扩张脊南端，这样的构造位置特征是大陆边缘／扩张脊汇聚的结果导致的。现今对拉普捷夫海陆架地质的理解是基于俄罗斯多道地震反射数据和陆地地质学的推断。地质与板块... 详细信息

拉普捷夫海对于研究大陆初始张裂的过程具有重要意义。它位于加克尔扩张脊南端，这样的构造位置特征是大陆边缘／扩张脊汇聚的结果导致的。现今对拉普捷夫海陆架地质的理解是基于俄罗斯多道地震反射数据和陆地地质学的推断。地质与板块运动学数据常用来约束对地震反射数据的解释。拉普捷夫海裂谷体系由几个深下切裂谷和基底的高位区块组成。自西向东分别是：西拉普捷夫海和南拉普捷夫海裂谷盆地、Ust’Lena裂谷、东拉普捷夫海和斯托尔博沃伊地垒、Bel’kov-svyatoi nos和Anisin裂谷。陆架的中部和东部其重力场具有很大的差异，从裂谷上的60mGal到地垒上的50mGal。拉普捷夫海裂谷包含五个地震层序单元，它们以清晰的区域反射面为界，在它们下面为非均质的褶皱基底。这些层序单元的年龄为晚自垩世至全新世，反映了扩张脊／大陆边缘相互作用的不同阶段。与裂谷相关的沉积物总厚度估计在4km和8—10km之间，而在隆起之上的沉积盖层的厚度明显要薄些，一般不超过1～2km。从南拉普捷夫海盆约10km处到Bel’kov-svyatoi nos裂谷的4～5km处，沉积区总厚度呈向东减小的趋势，整个裂谷构造的单一性可能表明同一个方向上裂谷的再次发育。整个裂谷系统被最上部的地震单元覆盖，这可能反映出自约2Ma年来北美／欧亚板块最后一次重组期间裂谷作用开始减速。

关键词：拉普捷夫海欧亚海盆地堑地垒正断层地震层序沉积盆地伸展构造