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multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications

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microsystems & Nanoengineering 2024年第2期10卷 207-218页

作者： Preksha Gupta Ambili mohan Apurv mishra Atindra Nair Neeladri chowdhury Dhanush Balekai Kavyashree Rai Anil Prabhakar Taslimarif saiyed Discovery Innovation Accelerator Centre for Cellular and Molecular PlatformsBellary RoadBengaluru560065KarnatakaIndia Department of Electrical Engineering Indian Institute of Technology MadrasChennai600036Tamil NaduIndia

Droplet microfluidics has emerged as a critical component of several high-throughput single-cell analysis techniques in biomedical research and *** significant progress in the development of individual assays,multiparametric optical sensing of droplets and their encapsulated contents has been *** current approaches,most commonly involving microscopy-based high-speed imaging of droplets,are technically complex and require expensive instrumentation,limiting their widespread *** address these limitations,we developed the OptiDrop platform;this platform is a novel optofluidic setup that leverages the principles of flow *** platform enables on-chip detection of the scatter and multiple fluorescence signals from the microfluidic droplets and their contents using optical *** highly customizable on-chip optical fiber-based signal detection system enables simplified,miniaturized,low-cost,multiparametric sensing of optical signals with high sensitivity and single-cell resolution within each *** demonstrate the ability of the OptiDrop platform,we conducted a differential expression analysis of the major histocompatibility complex(mHC)protein in response to IFNγ*** results showed the platform’s ability to sensitively detect cell surface biomarkers using fluorescently labeled ***,the OptiDrop platform combines the versatility of flow cytometry with the power of droplet microfluidics to provide wide-ranging,scalable optical sensing solutions for research and diagnostics.

关键词： scatter enable resolution

^(4)He monolayer on graphene:a quantum monte Carlo study

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Communications in Theoretical Physics 2024年第9期76卷 149-157页

作者： s Yu m Boninsegni Department of Physics University of AlbertaEdmontonAlbertaT6G 2H5Canada

We revisit the problem of adsorption of a single^(4)He layer on graphene,focusing on the commensurate(C_(1/3))crystalline phase,specifically on whether it may possess a nonzero superfluid response,and on the existence of superfluid phases,either(metastable)liquid or vacancy-doped *** make use of canonical quantum monte Carlo simulations at zero and finite temperature,based on a realistic microscopic model of the *** results confirm the absence of any superfluid response in the commensurate crystal,and that no thermodynamically stable uniform phase exists at lower *** evidence of a possibly long-lived,metastable superfluid phase at C_(1/3)coverage is ***,the results of ground-state projection methods and finite-temperature simulations are entirely consistent.

关键词： superfluidity low-dimensional systems quantum monte Carlo helium supersolids

Direct microwave leaching conditions of rare earth elements in fluorescent wastes

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Journal of Rare Earths 2024年第6期42卷 1165-1174,I0007页

作者： A.Bilen B.Birol m.N.saridede s.s.Kaplan m.s.sonmez Department of metallurgical and materials Engineering Yildiz Technical UniversityIstanbulTurkey Department of Chemical Engineering and materials science Stevens Institute of TechnologyNew JereyUSA Department of metallurgical and materials Engineering Istanbul Technical UniversityIstanbulTurkey

The luminescent phosphor powder in the fluorescent lamp constitutes 2%of the lamp’s *** can be mentioned that fluorescent wastes are a crucial raw material to produce rare earth *** the present study,microwave leaching process was conducted to dissolve rare earth elements yttrium(Y),europium(Eu),and remaining rare earth elements(REEs)present in the phosphor powder of the fluorescent *** the yields were *** the microwave leaching process,the effects of the temperature(80-160℃),acid type(hydrochloric acid(HCl),nitric acid(HNO_(3)),sulphuric acid(H_(2)sO_(4))),acid concentration(0.5-6 mol/L),solid to liquid ratio(0.1:10-0.5:10)and reaction time(5-90 min)parameters on leaching efficiencies of varying rare earth elements and calcium were *** highest yield was obtained in the direct microwave leaching of fluorescent waste with the experimental conditions of 6 mol/L HCl,160℃,0.1:10 solid-to-liquid ratio(s:L),and 90 *** energy calculations were made,and kinetic models of the reactions were obtained,and it is observed that Y and Eu dissolution is diffusion-controlled,on the other hand,lanthanum(La),cerium(Ce),and terbium(Tb)were examined to be chemical reaction ***,calcium(Ca)and gadolinium(Gd)seem coherent with the mixed ***,mathematical models of all experimental studies are created with the response surface Box-Behnken method and the correlation coefficients of all the models are over 90%.

关键词： Rare earth elements(REEs) Direct microwave leaching Fluorescent waste Kinetic models Response surface method

Is intraprocedural intravenous aspirin safe for patients who require emergent extracranial stenting during mechanical thrombectomy?

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stroke & Vascular Neurology 2024年第3期9卷 279-288,I0247页

作者： Adam Ingleton marko Raseta Rui-En Chung Kevin Jun Hui Kow Jake Weddell sanjeev Nayak Changez Jadun Zafar Hashim noman Qayyum Phillip Ferdinand Indira Natarajan Christine Roffe Neurosciences University Hospitals of North Midlands NHS TrustStoke-on TrentUK statistics and mathematical modelling Department of Molecular GeneticsErasmus Medical CenterRotterdamZuid-HollandThe Netherlands Radiology University Hospitals of North Midlands NHS TrustStoke-on TrentUK school of medicine Keele UniversityKeeleUK Interventional Radiology University Hospitals of North Midlands NHS TrustStoke-on TrentUK stroke Research Keele UniversityKeeleUK

Background Intraoperative antiplatelet therapy is recommended for emergent stenting during mechanical thrombectomy(mT).most patients undergoing mT are also given *** agents are contraindicated within 24 hours of *** evaluated outcomes and complications of patients stented with and without intravenous aspirin during *** All patients who underwent emergent extracranial stenting during mT at the Royal stoke University Hospital,UK between 2010 and 2020,were *** were thrombolysed before mT,unless *** 500 mg intravenously was given intraoperatively at the discretion of the *** intracranial haemorrhage(sICH)and the National Institutes for Health stroke scale score(NIHss)were recorded at 7 days,and mortality and functional recovery(modified Rankin scale:mRs≤2)at 90 *** Out of 565 patients treated by mT 102 patients(median age 67 IQR 57–72 years,baseline median NIHss 18 IQR 13–23,76(75%)thrombolysed)had a stent *** these 49(48%)were given aspirin and 53(52%)were *** treated with aspirin had greater NIHss improvement(median 8 IQR 1–16 vs median 3 IQR-9–8 points,p=0.003),but there were no significant differences in sICH(2/49(4%)vs 9/53(17%)),mRs≤2(25/49(51%)vs 19/53(36%))and mortality(10/49(20%)vs 12/53(23%))with and without *** improvement(median 12 IQR 4–18 vs median 7 IQR-7–10,p=0.01)was greater,and mortality was lower(4/33(12%)vs 6/15(40%),p=0.05)when aspirin was combined with thrombolysis,than for aspirin alone,with no increase in *** Our findings based on registry data derived from routine clinical care suggest that intraprocedural intravenous aspirin in patients undergoing emergent stenting during mT does not increase sICH and is associated with good clinical outcomes,even when combined with intravenous thrombolysis.

关键词： patients aspirin intravenous

spatio-temporal variation and relationship between agricultural efficiency and irrigation intensity in a semi-arid region of India

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Regional sustainability 2024年第2期5卷 123-139页

作者： shibu DAs Kaushal Kumar sHARmA suranjan mAJUmDER Debabrata DAs Indrajit Roy chowdhury Centre for the study of Regional Development School of Social SciencesJawaharlal Nehru UniversityNew Delhi110067India Department of Geography&Applied Geography University of North BengalDarjeeling734013India Geography Department of Krishnagar Government College Krishnagar741101India

A surging population in Karnataka state,a semi-arid region in India,poses a threat to both food security and livelihood sustainability,necessitating a concentrated effort to bolster agricultural efficiency and achieve United Naton’s sustainable Development Goal 2(zero hunger).Therefore,in order to address the pressing issue of food scarcity in Karnataka,this study meticulously examined the spatio-temporal variation of agricultural efficiency and irrigation intensity in Karnataka,uncovering its significant dependence of agricultural efficiency on irrigation ***,this study used a one-way analysis of variance(ANOVA)to ascertain significant differences in the means of agricultural efficiency and irrigation intensity during 2004-2005 and *** study showed that the maximum improvement in agricultural efficiency index was recorded in Belgaum(40.24),Gulbarga(24.77),and Yadgir districts(22.92)between 2004-2005 and 2018-2019,which indicated the progressing trend and better scope for agriculture *** the contrary,some districts expressed threat(a decline of above 20.00 of agricultural efficiency index)and needed special care for the improvement of agricultural efficiency in four northern districts(Bagalkot,Bidar,Raichur,and Bijapur),three southern districts(Chitradurga,Chikballapur and Hassan),and two southern districts(Koppal and Gadag)in *** 2004-2005,irrigation intensity varied from 3.19%to 56.39%,with the lowest irrigation intensity in Kodagu District and the highest irrigation intensity in shimoga *** 2018-2019,irrigation intensity changed from 0.77%to 72.77%,with the lowest irrigation intensity in Kodagu District and the highest in Dakshin Kannad ***,the research scrutinized the complex relationship between agricultural efficiency and irrigation intensity,with the correlation coefficient increased from 0.162 during 2004-2005 to 0.255 during *** implies that in both periods,a low positive

关键词： Agricultural efficiency Irrigation intensity One-way analysis of variance(ANOVA) Food security Karnataka

Bioelectronic medicine in modulation of cortical spreading depolarization and beyond

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Neural Regeneration Research 2025年第2期20卷 481-482页

作者： khaled Alok Timothy G.White Chunyan Li Department of Neurosurgery Donald and Barbara Zucker School of Medicine at Hofstra/NorthwellHempsteadNYUSA Translational Brain Research Laboratory The Feinstein Institutes for Medical ResearchManhassetNYUSA

Bioelectronic interventions,specifically trigeminal nerve st imulat ion(TNs),have attracted considerable attention in conditions where cortical spreading depolarizations(CsDs)accompanied by compromised cerebral perfusion may exacerbate neurological *** pharmacological interventions have demonstrated initial potential in addressing CsDs,a standardized treatment approach has not yet been *** objective of this perspective is to explore emerging bioelectronic methodologies for addressing CsDs,particularly emphasizing TNs,and to underscore TNs’s capacity to enhance neurovascular coupling and cerebral perfusion.

关键词： cerebral perfusion electronic

Parametric investigation on the novel and cost-effective nano fly ash impregnated geopolymer system for sustainable construction

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Frontiers of structural and Civil Engineering 2024年第2期18卷 170-183页

作者： R.mOHANA s.m.Leela BHARATHI Department of Civil Engineering Mepco Schlenk Engineering CollegeSivakasi 626123India

The hazardous environmental effects of greenhouse gas emissions and climate change demand alternative sources for cementitious materials in the construction *** development of geopolymer structures provides a way of producing 100%cement-free *** this research work,a novel and simple way of deriving nano particles from waste fly ash particles is *** effect of adding the synthesized nano fly ash particles as a filler medium in geopolymer mortars was investigated by considering strength and durability *** optimization was done by using regression analysis on the geopolymer mortar and the impact of adding nano fly ash particles was studied by varying different percentages of addition ranging from 0 to 7.5%by weight of binder *** the results,it was observed that 1%nano fly ash acted not only as a filler but also as nano-sized precursors of the polymerization process,resulting in denser geopolymer *** can explain the extraordinary gain in strength of 72.11 mPa as well as the denser core with negligible level of chloride ion penetration,making the material suitable for the development of structures susceptible to marine environment.

关键词： geopolymer nano fly ash chloride ion penetration durability polymerization fillers

QCD sum rule study of J^(P)=1^(±)exotic states with two heavy quarks in the molecular picture

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Chinese Physics C 2024年第6期48卷 31-37页

作者： T.m.Aliev s.Bilmis m.savcı Department of Physics Middle East Technical UniversityAnkara06800Turkey TUBITAK ULAKBIm Ankara06510Turkey

In this study,the spectroscopic parameters of exotic molecular states composed of mesons containing two heavy quarks(scalar-axial and pseudoscalar-axial meson combinations)are investigated within the QCD sum *** findings reveal that molecular states containing charm quarks do not form bound states,whereas states with b-quarks can form exotic molecular *** observation has significant implications for understanding the structure of these exotic states.

关键词： QCD sum rules exotic mesons molecular states

Analysis of strong decays of sU(3)partners ofΩ(2012)baryon

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Chinese Physics C 2024年第8期48卷 34-41页

作者： T.m.Aliev s.Bilmis m.savci Department of Physics Middle East Technical UniversityAnkara 06800Turkey TUBITAK ULAKBIm Ankara 06510Turkey

We estimate the coupling constants and decay widths of the sU(3)partners of theΩ(2012)hyperon,as discovered by the BELLE Collaboration,using the distribution amplitudes of the octet baryons within the light cone sum rules *** study includes a comparison of the obtained results for the relevant decay widths with those derived within the framework of the flavor sU(3)*** observe a good agreement between the predictions of both ***,our result on the decay width ofΩ→ΞK is compatible with the existing experimental result within the uncertainties of the model *** results can provide helpful insights for determining the nature of the sU(3)partners of theΩ(2012)baryon.

关键词： coupling constants light cone sum rule hyperon