检索结果-南通市图书馆

Morphophysiological Diversity and Haplotype Analysis of saltol QTl Region in Diverse Rice landraces for salinity Tolerance

引用

Rice science 2023年第4期30卷 306-320,I0010-I0012页

作者： B.M.lOKEsHKUMAR S.L.KRISHNAmURTHY suman RATHOR Arvinder singh WARRIACH N.M.VINAYKUMAR B.M.DUsHYANTHAKUMAR Parbodh Chander sHARMA Division of Crop Improvement Central Soil Salinity Research InstituteKarnal-132001HaryanaIndia Department of Biotechnology Kuvempu UniversityShankaraghattaShimvamogga-577451KarnatakaIndia Department of Genetics and Plant Breeding University of Agricultural and Horticultural ScienceShimvamogga-577204KarnatakaIndia

Rice is sensitive to salinity stress at both the seedling and reproductive stages.The present study used 145 rice genotypes comprising of 100 landraces and 45 advanced breeding lines collected from different regions of India.These genotypes were evaluated in hydroponics under control[electrical conductivity(ECe)~1.2 ds/m]and saline(ECe~10.0 ds/m)environments along with susceptible(IR29)and tolerant(Fl478)checks.The stress susceptibility index for eight morphophysiological traits was estimated.Analysis of variance showed significant differences among the genotypes for all the parameters studied in control,stress and relative stress conditions.We identified 3 landraces(Kuttimanja,Tulasimog and IET-13713I)as tolerant and 14 lines as moderately tolerant to salt stress.strong correlations in the morphological(root and shoot lengths)and physiological traits(shoot Na^(+),Ca^(2+)and Mg^(2+)contents,and Na^(+)/K^(+)ratio)were observed under all the conditions.The hierarchical cluster analysis grouped the genotypes into five clusters,among which cluster Ⅱ comprised salt-tolerant lines.Haplotyping of saltol region using 11 simple sequence repeat markers on 17 saline tolerant and moderately tolerant lines was conducted.Markers AP3206F,RM10793 and RM3412b,located close to sKC1 gene(11.23‒12.55 Mb),displayed diverse allelic variations and they were not related to the Fl478 type.In this region,tolerant lines like Kuttimanja,IET-13713I and Tulasimog have new alleles.As a result,these lines may be suitable candidates for novel genomic regions governing rice salinity tolerance.salt-tolerance ability of Kuttimanja,Tulasimog and IET-13713I was validated in two years in three salinity stress environments.These promising lines can be used in breeding programs to broaden the genetic base of salinity tolerance in rice,and it may help to dissect key genomic regions responsible for salinity tolerance.

关键词： haplotype salinity tolerance rice saltol diversity sKC1 gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Haplotyping of Rice Genotypes Using simple sequence Repeat Markers Associated with salt Tolerance

引用

Rice science 2016年第6期23卷 317-325页

作者： A.D.CHOWDHURY G.HARITHA T.sUNITHA S.L.KRISHNAmURTHY B.DIVYA G.PADMAVATHI T.RAM N.sARlA ICAR-Indian Institute of Rice Research Division of Crop Improvement Central Soil Salinity Research Institute

salt stress is a major problem in most of the rice growing areas in the world. A major QTlsaltol associated with salt tolerance at the seedling stage has been mapped on chromosome 1 in rice.This study aimed to characterize the haplotype diversity at saltol and additional QTls associated withsalt tolerance. salt tolerance at the seedling stage was assessed in 54 rice genotypes in the scale of 1to 9 score at EC = 10 dsm^-1 under controlled environmental conditions. seven new breeding linesincluding three KMR3/O. rufipogon introgression lines showed similar salt tolerant ability as Fl478 andcan be good sources of new genes/alleles for salt tolerance. simple sequence repeat （ssR） markerRM289 showed only two alleles and RM8094 showed seven alleles. Polymorphic information contentvalue varied from 0.55 for RM289 to 0.99 for RM8094 and RM493. Based on 14 ssR markers, the 54lines were clearly separated into two major clusters. Fourteen haplotypes were identified based onsaltol linked markers with Fl478 as the reference. Alleles of RM8094 and RM3412 can discriminatebetween the salt tolerant and susceptible genotypes clearly and hence can be useful in marker-assistedselection at the seedling stage. Other markers RM10720 on chromosome 1 and RM149 and RM264 onchromosome 8 can also distinguish tolerant and susceptible lines but with lesser stringency.

关键词： haplotype rice salt tolerance saltol simple sequence repeat marker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

活细胞中的化学物理-光学观测和控制细胞内信号转导（英文）

引用

Chinese Journal of Chemical Physics 2018年第4期31卷 375-392,613页

作者： Vishnu V.krishnamurthy 张凯伊利诺伊大学厄巴纳-香槟分校生物化学系伊利诺伊大学厄巴纳-香槟分校生物物理和定量生物学研究中心伊利诺伊大学厄巴纳-香槟分校神经科学研究中心

在活细胞拥挤的微环境中生物大分子之间存在着不间断的物理和化学相互作用．细胞功能直接取决于其内部的分子组成和分子间相互作用．不仅不同种细胞之间的化学和物理性质千差万别，同一细胞内的物理特性也会受环境影响不断产生动态变化... 详细信息

在活细胞拥挤的微环境中生物大分子之间存在着不间断的物理和化学相互作用．细胞功能直接取决于其内部的分子组成和分子间相互作用．不仅不同种细胞之间的化学和物理性质千差万别，同一细胞内的物理特性也会受环境影响不断产生动态变化．而这种动态变化的化物特性对于细胞适应环境的改变至关重要．通常环境的改变会引起细胞产生不同响应，比如改变其基因的转录和翻译．是否形成正确的细胞响应取决于细胞对外部环境信息的准确解读．而这种解读通常是通过细胞内信号转导途径来实现．一个化学或物理的信号会通过刺激一系列细胞内的生化反应将外部环境的信息准确地传递到细胞内部．由于信号传导过程经常需要穿跨子亚细胞空间的复杂路径，所以细胞内信号转导的实际功用就是一座连接外部环境与细胞内部的桥梁．了解信号分子在细胞微环境中的化物状态将有助于阐释细胞功能．当今实验和理论工具的发展己使得在活细胞这样的微环境中探索纷繁的分子化物性质成为可能．本文总结如何利用新兴的光学显微镜和光遗传技术去观测甚至控制生物信号在细胞内的传导过程．确信这些技术己经为精确控带l_生物体中分子的化学和物理性质提供了前断未有的机遇．希望本文可以为有兴趣研究生物和医学问题的化学物理研宄人员抛砖引玉．

关键词：光遗传学信号转导光学显微镜超分辨率成像蛋白质与蛋白质相互作用受体细胞骨架细胞物质转运基因转录与翻译

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phenology of a tropical dry deciduous forest of Bhadra wildlife sanctuary,southern India

引用

Ecological Processes 2014年第1期3卷 263-274页

作者： Appaji Nanda Hebbalalu s suresh Yelugere l krishnamurthy Department of Postgraduate studies and Research in Applied Botany Bioscience ComplexJnana SahyadriKuvempu UniversityShankaraghattaShimoga 577451KarnatakaIndia Biodiversity Education and Research laboratory Environmental Study CentreSanthekaduruShimoga 577222KarnatakaIndia Centre for Ecological sciences Indian Institute of ScienceBangalore 560012KarnatakaIndia

Introduction:This paper describes the leafing,flowering and fruiting phenology of canopy trees in the dry deciduous forest of Bhadra wildlife sanctuary from June 2004 to May 2006.Method:All the woody canopy individuals(>20 cm girth at breast height)were identified and tagged with a unique number along a transect of approximately 2 Km comprising 157 individuals of 22 species.Observations were made at monthly intervals from June 2004 to May 2006 for leafing,flowering and fruiting phenophases.Result:leaf fall starts in september,with a peak in December and January.leaf initiation begins in February,with a peak in April before the monsoon.leaf expansion starts in February from pre-monsoon with a peak in May and July during the monsoon.leaf senescence begins in september to November and peaks in January to March.Flower bud initiates in January with a peak in April and May,and pollination begins in April with a peak in May and July before the monsoon to onset of monsoon.Fruit bud initiates in May with a peak in september and October.Unripened fruit was observed in May with a peak in september and November.Fruit fall begins in November with a peak in March.Conclusion:Our results show that leafing and flowering activities occur in the summer or pre-monsoon.Fruiting patterns occur during the monsoon to post-monsoon season.seasonality among various phenophases indicates that leaf senescence flower initiation and fruit fall have strong seasonality.

关键词： Bhadra wildlife sanctuary Dry deciduous forest Phenophases Rayleigh’s Z test seasonality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biodiversity of endophytic fungi from seven herbaceous medicinal plants of Malnad region, Western Ghats, southern India

引用

Journal of Forestry Research 2014年第3期25卷 707-711页

作者： B.shankar Naik M.Krishnappa Y.l.krishnamurthy Department of P.G. studies and Research in Applied Botany Kuvempu University Govt. science College Department of BiologyBadami

A total of 3611 fungal isolates were recovered from 4200 leaf segments incubated from 7 medicinal herbs during monsoon, winter and summer seasons. These fungal isolates belonged to teleomorphic Asco-mycota （23.5%）, anamorphic Ascomycota producing conidiomata （17.4%）, anamorphic Ascomycota without conidiomata （46.9%）, Zygo-mycota （1.42%） and sterile forms （10.6%）. Chaetomium globosum, As-pergillus niger, Aureobasidium pullulans, Curvularia lunata, Fusarium spp., Penicillium spp., Pestalotiopsis spp., Trichoderma viridae, Cladosporium cladosporioides, were frequently isolated from more than one host plant. The number of endophytic isolates was higher in winter than in monsoon and summer seasons.

关键词： endophytes diversity herbs seasonality Malnad region

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Opportunities and challenges in developing tissue-agnostic anti-cancer drugs

引用

Journal of Cancer Metastasis and Treatment 2020年第1期6卷 131-147页

作者： Ivan W.li Nithya krishnamurthy Ge Wei Gary li Current affiliation:Canyon Crest Academy San DiegoCA 92130USA Current affiliation:Yale University New HavenCT 06520USA Translational Research IgnytaInc.San DiegoCA 92121USA Current affiliation:Translational Medicine QED TherapeuticsSan FranciscoCA 94107USA

The rapid advances in the understanding of oncogenic process and the maturation of affordable precision diagnostic tools have enabled the development of targeted therapeutic agents,such as those targeting BCR-ABl,epithelial growth factor receptor l858R,EMl4-anaplastic lymphoma kinase,and BRAF V600E,to treat cancers that harbor specific molecular alterations.Traditionally,each targeted drug has been developed for a particular tumor type where such alteration is most frequently found.Recently,the widespread adoption of next generation sequencing has led to an increase in the identification of rare and ultra-rare alterations,and,in some cases,the same rare alterations are found across multiple tumor types.The rarity of these alterations makes clinical trials traditionally designed for specific tumor types infeasible.As a result,tissue-agnostic trials have been developed to study the efficacy of these treatments and increase patient access.This review summarizes current successful cases of tissue-agnostic development,such as drugs targeting tropomyosin receptor kinase fusions,and proposes the next wave of potential tissue-agnostic targets,including fusions of ROs1,anaplastic lymphoma kinase,fibroblast growth factor receptor,and rearranged during transfection.In addition,the advantages and the challenges of such approach are discussed in the context of clinical development and approval.

关键词： Tissue agnostic basket trial tropomyosin receptor kinase anaplastic lymphoma kinase ROs1 fibroblast growth factor receptor rearranged during transfection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hydroxyapatite-containing PEO-coating design for biodegradable Mg-0.8Ca alloy: Formation and corrosion behaviour

引用

Journal of Magnesium and Alloys 2023年第12期11卷 4468-4484页

作者： A.s.Gnedenkov s.l.sinebryukhov V.s.Filonina s.V.Gnedenkov Institute of Chemistry of FEB RAs 159 Pr.100-letiya VladivostokaVladivostok690022Russian Federation

In this study, the biocompatible protective coating was formed using plasma electrolytic oxidation(PEO) on bioresorbable Mg-0.8Ca alloy. The composition of the formed coating was studied using XRD, sEM-EDX analysis, and micro-Raman spectroscopy. The uniform distribution of hydroxyapatite over the thickness of protective PEO-layer was established. Using traditional(EIs, PDP, OCP) and local scanning electrochemical methods(sVET, sIET with H^(+)-selective microelectrode), the level of protective properties of PEO-layer in a biological environment(mammalian cell culture medium, MEM) was determined. It was established that modification of Mg-0.8Ca alloy surface by PEO contributes to a significant increase in the corrosion resistance of the surface layer, making it possible to control the process of material‘s biodegradation. The maximum local electrochemical activity was recorded after 72 h of testing, while for the uncoated sample,intense corrosion degradation was recorded in the first 12 min of exposure to the cell culture medium. Formation of the PEO-coating results in a twofold decrease in the corrosion current density(2.8·10^(-6)A cm^(-2)) and an increase in the impedance modulus measured at a low frequency(1.7·10^(4)Ω cm^(2)) in comparison with the uncoated material(9.5·10^(-6)A cm^(-2);8.1·10^(3)Ω cm^(2)). The mechanism of material bioresorption was established and a model for biodegradation process of Mg-0.8Ca alloy with hydroxyapatite-containing PEO-coating in MEM was proposed. Analysis of these results and comparing with others obtained by various scientific groups indicate the prospects for application of biocompatible PEO-coating on Mg-Ca alloy in implant surgery.

关键词： Magnesium-calcium alloy Biodegradation Implant surgery Plasma electrolytic oxidation Electrochemistry Minimum essential medium Hydroxyapatite

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Abiotic and biotic constituents of oil sands process-affected waters

引用

Journal of Environmental sciences 2023年第5期127卷 169-186页

作者： Nora A.s.Hussain James l.stafford Department of Biological sciences University of AlbertaEdmontonAlberta T6G 2N8Canada

The oil sands in Northern Alberta are the largest oil sands in the world,providing an important economic resource for the Canadian energy industry.The extraction of petroleum in the oil sands begins with the addition of hot water to the bituminous sediment,generating oil sands process-affected water(OsPW),which is acutely toxic to organisms.Trillions of litres of OsPW are stored on oil sands mining leased sites in man-made reservoirs called tailings ponds.As the volume of OsPW increases,concerns arise regarding the reclamation and eventual release of this water back into the environment.OsPW is composed of a complex and heterogeneous mix of components that vary based on factors such as company extraction techniques,age of the water,location,and bitumen ore quality.Therefore,the effective remediation of OsPW requires the consideration of abiotic and biotic constituents within it to understand short and long term effects of treatments used.This review summarizes selected chemicals and organisms in these waters and their interactions to provide a holistic perspective on the physiochemical and microbial dynamics underpinning OsPW.

关键词： OsPW toxicants Reclamation Tailings ponds Remediation Microbial AOPs Bioremediation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of process parameters on microstructural and mechanical properties of Ti.ss 304l explosive cladding

引用

Journal of Central south University 2017年第6期24卷 1245-1251页

作者： saravanan somasundaram Raghukandan krishnamurthy Hokamoto Kazuyuki Department of Mechanical Engineering Annamalai UniversityAnnamalai NagarTamilnadu 608002India Department of Manufacturing Engineering Annamalai UniversityAnnamalai NagarTamilnadu 608002India Institute of Pulsed Power science Kumamoto UniversityKurokamiKumamotoJapan

The present work pertains to the study of microstructure and mechanical properties of explosively cladded commercially pure titanium(cpTi) with austenitic stainless steel(ss 304l) subjected to varied process parameters, viz., loading ratios(mass of explosive or mass of flyer plate) and preset angles. The microstructural study reveals the transformation from straight to wavy interface while increasing loading ratios. Vickers hardness increases with loading ratios, and the maximum hardness is witnessed in the closer proximity of collision interface. Ram tensile and shear strength of explosive claddings are higher than that of parent plates. While the base plate fails in impact test, the flyer plate is deformed, indicating good impact strength. Increased mass of claddings, due to oxide formation, is witnessed in corrosion tests, which confirms the superiority of explosive claddings in corrosive environment. Triaxial weldability window, an analytical estimation for Ti-ss 304l explosive claddings, is developed and correlated.

关键词： explosive cladding titanium stainless steel microstructure strength corrosion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Autologous tissue patch rich in stem cells created in the subcutaneous tissue

引用

World Journal of stem Cells 2015年第8期7卷 1127-1136页

作者： Ignacio Garcia-Gomez krishnamurthy P Gudehithlu Jose A l Arruda Ashok K singh Division of Nephrology Hektoen Institute of Medicine John H.stroger Jr.Hospital of Cook County Division of Nephrology Department of MedicineUniversity of Illinois Medical Center at Chicago and the Chicago VA Medical Center

AIM:To investigate whether we could create natural autologous tissue patches in the subcutaneous space for organ repair. METHODs: We implanted the following three types of inert foreign bodies in the subcutaneous tissue of rats to produce autologous tissue patches of different geometries:(1) a large-sized polyvinyl tube(l = 25 mm,internal diameter = 7 mm) sealed at both ends by heat application for obtaining a large flat piece of tissue patch for organ repair;(2) a fine polyvinyl tubing(l = 25 mm,internal diameter = 3 mm) for creating cylindrically shaped grafts for vascular or nerve repair; and(3) a slurry of polydextran particle gel for inducing a bladder-like tissue. Implantation of inert materials was carried out by making a small incision on one or either side of the thoracic-lumbar region of rats. subcutaneous pockets were created by blunt dissection around the incision into which the inert bodies were inserted(1 or 2 per rat). The incisions were closed with silk sutures,and the animals were allowed to recover. In case of the polydextran gel slurry 5 m l of the slurry was injected in the subcutaneous space using an 18 gauge needle. After implanting the foreign bodies a newly regenerated encapsulating tissue developed around the foreign bodies. The tissues were harvested after 4-42 d of implantation and studied by gross examination,histology,and histochemistry for organization,vascularity,and presence of mesenchymal stem cells(MsCs)(CD271+CD34+ cells). REsUlTs: Implanting a large cylindrically shaped polyvinyl tube resulted in a large flat sheet of tissue that could be tailored to a specific size and shape for use as a tissue patch for repairing large organs. Implanting a smaller sized polyvinyl tube yielded a cylindrical tissue that could be useful for repairing nerves and blood vessels. This type of patch could be obtained in different lengths by varying the length of the implanted tube. Implanting a suspension of inert polydextran suspension gave rise to a bladder-like tissue that could be potentially use

关键词： Autologous tissue patch Foreign body subcutaneous

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：