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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos santos P.J.Dijkstra C.Mota J.Bauer h.Ferreira Rui L.Reis N.Neves s.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVs/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBAsQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMsCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending surface functionalization surface density Click chemistry human

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scalable orthogonal delay-division multiplexed OEO artificial neural network trained for TI-ADC equalization

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Photonics Research 2024年第1期12卷 85-105页

作者： ANDREA ZAZZI ARKA DIPTA DAs LUKAs hüssEN RENATO NEGRA JEREMY WITZENs Institute of Integrated Photonics RWTH Aachen University52074 AachenGermany Chair of high-Frequency Electronics RWTH Aachen University52074 AachenGermany

We propose a new signaling scheme for on-chip optical-electrical-optical artificial neural networks that utilizes orthogonal delay-division multiplexing and pilot-tone-based self-homodyne *** scheme offers a more efficient scaling of the optical power budget with increasing network *** simulations,based on220 nm silicon-on-insulator silicon photonics technology,suggest that the network can support 31×31 neurons,with 961 links and freely programmable weights,using a single 500 m W optical comb and a signal-to-noise ratio of 21.3 d B per ***,it features a low sensitivity to temperature fluctuations,ensuring that it can be operated outside of a laboratory *** demonstrate the network’s effectiveness in nonlinear equalization tasks by training it to equalize a time-interleaved analog-to-digital converter(ADC)architecture,achieving an effective number of bits over 4 over the entire 75 Ghz ADC *** anticipate that this network architecture will enable broadband and low latency nonlinear signal processing in practical settings such as ultra-broadband data converters and real-time control systems.

关键词： network delay artificial

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The soft X-ray Imager(sXI)on the sMILE Mission

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Earth and Planetary Physics 2024年第1期8卷 5-14页

作者： s.sembay A.L.Alme D.Agnolon T.Arnold A.Beardmore A.Belén Balado Margeli C.Bicknell C.Bouldin G.Branduardi-Raymont T.Crawford J.P.Breuer T.Buggey G.Butcher R.Canchal J.A.Carter A.Cheney Y.Collado-Vega h.Connor T.Crawford N.Eaton C.Feldman C.Forsyth T.Frantzen G.Galgóczi J.Garcia G.Y.Genov C.Gordillo h-P.Gröbelbauer M.Guedel Y.Guo M.hailey D.hall R.hampson J.hasiba O.hetherington A.holland s-Y.hsieh M.W.J.hubbard h.Jeszenszky M.Jones T.Kennedy K.Koch-Mehrin s.Kögl s.Krucker K.D.Kuntz C.Lakin G.Laky O.Lylund A.Martindale J.Miguel Mas hesse R.Nakamura K.Oksavik N.Østgaard h.Ottacher R.Ottensamer C.Pagani s.Parsons P.Patel J.Pearson G.Peikert F.s.Porter T.Pouliantis B.h.Qureshi W.Raab G.Randal A.M.Read N.M.M.Roque M.E.Rostad C.Runciman s.sachdev A.samsonov M.soman D.sibeck s.smit J.søndergaard R.speight s.stavland M.steller TianRan sun J.Thornhill W.Thomas K.Ullaland B.Walsh D.Walton C.Wang s.Yang school of Physics and Astronomy University of LeicesterLeicesterLE17RHUK space Park Leicester University of LeicesterLeicesterLE17RHUK Mullard space science Laboratory University College LondonDorkingRH56NTUK Centre for Electronic Imaging Open UniversityMilton KeynesMK76 AAUK Birkeland Centre for space science Department of Physics and TechnologyUniv.of BergenN-5007 BergenNorway University of Vienna A-1010 ViennaAustria space Research Institute Austrian Academy of SciencesA-8042 GrazAustria National Institute of Aerospace Technology MadridSpain EsTEC European Space Agency2201 AZ NoordwijkNetherlands state Key Laboratory of space Weather National Space Science CentreBeijing 100190China University of Applied sciences and Arts Northwestern switzerland CH-5210 WindischSwitzerland Koegl space Gmbh CH-8157 DielsdorfSwitzerland space Acoustics Gmbh CH-8157 DielsdorfSwitzerland Zurich University of Applied sciences 8400 WinterhurSwitzerland Genov solutions N-5172 LoddefjordNorway sTM Engineering N-5165 LaksevagNorway John hopkins University Applied Physics Laboratory MD 20723USA NAsA/Goddard space Flight Centre MD 20771USA Center for space Physics Boston UniversityBostonMA 02215USA Department of Theoretical Physics and Astrophysics Masaryk University60177 BrnoCzech Republic Institute of Physics E¨otvo¨s Lor´and University1053 BudapestHungary Aerospace Information Research Institute Chinese Academy of SciencesBeijing 100045China Centro de Astrobiologia(CAB) CSIC-INTAMadridSpain

The soft X-ray Imager(sXI)is part of the scientific payload of the solar wind Magnetosphere Ionosphere Link Explorer(sMILE)*** is a joint science mission between the European space Agency(EsA)and the Chinese Academy of sciences(CAs)and is due for launch in *** is a compact X-ray telescope with a wide field-of-view(FOV)capable of encompassing large portions of Earth’s magnetosphere from the vantage point of the sMILE *** is sensitive to the soft X-rays produced by the solar Wind Charge eXchange(sWCX)process produced when heavy ions of solar wind origin interact with neutral particles in Earth’s *** provides a mechanism for boundary detection within the magnetosphere,such as the position of Earth’s magnetopause,because the solar wind heavy ions have a very low density in regions of closed magnetic field *** sensitivity of the sXI is such that it can potentially track movements of the magnetopause on timescales of a few minutes and the orbit of sMILE will enable such movements to be tracked for segments lasting many *** is led by the University of Leicester in the United Kingdom(UK)with collaborating organisations on hardware,software and science support within the UK,Europe,China and the United states.

关键词： soft X-ray Imaging micropore optics large area CCD

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Early Phases of star Formation: Testing Chemical Tools

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Research in Astronomy and Astrophysics 2024年第1期24卷 96-118页

作者： N.C.Martinez s.Paron CONICET-Universidad de Buenos Aires.Instituto de Astronomía y Física del Espacio Ciudad Universitaria(C1428EGA)Ciudad Autónoma de Buenos AiresArgentina

star-forming processes strongly influence the IsM ***,many high-quality databases are available at millimeter *** them,it is possible to carry out studies that review and deepen previous *** these studies involve large samples of sources,it is preferred to use direct tools to study the molecular *** the aim of testing these tools such as the use of the hCN/hNC ratio as a thermometer,and the use of h^(13)CO^(+),hC_(3)N,N_(2)h^(+) and C_(2)h as "chemical clocks," we present a molecular line study toward 55 sources representing massive young stellar objects at different evolutionary stages:infrared dark clouds(IRDCs),highmass protostellar objects(hMPOs),hot molecular cores(hMCs) and ultracompact h II *** found that the use of the hCN/hNC ratio as a universal thermometer in the IsM should be taken with care because the hCN optical depth is a big issue that can affect the ***,this tool should be utilized only after a careful analysis of the hCN spectrum,checking that no line,neither the main nor the hyperfine ones,presents absorption *** point out that the analysis of the emission of h^(13)CO^(+),hC_(3)N,N_(2)h^(+) and C_(2)h could be useful to trace and distinguish regions among IRDCs,hMPOs and *** molecular line widths of these four species increase from the IRDC to the hMC stage,which can be a consequence of the gas dynamics related to the starforming processes taking place in the molecular *** results not only contribute with more statistics,acting as a probe of such chemical tools,useful to obtain information in large samples of sources,but also complement previous works through the analysis of other types of sources.

关键词： stars:formation IsM:molecules IsM:clouds

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A novel saliva-based miRNA profile to diagnose and predict oral cancer

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International Journal of Oral science 2024年第1期16卷 97-109页

作者： Jaikrishna Balakittnen Chameera Ekanayake Weeramange Daniel F.Wallace Pascal h.G.Duijf Alexandre s.Cristino Gunter hartel Roberto A.Barrero Touraj Taheri Liz Kenny sarju Vasani Martin Batstone Omar Breik Chamindie Punyadeera saliva&Liquid Biopsy Translational Laboratory Griffith Institute for Drug DiscoveryGriffith UniversityNathanQLDAustralia Department of Medical Laboratory sciences Faculty of Allied Health SciencesUniversity of JaffnaJaffnaSri Lanka Menzies health Institute Griffith UniversityGold CoastQLDAustralia school of Biomedical sciences Faculty of HealthQueensland University of TechnologyBrisbaneQLDAustralia Centre for Cancer Biology Clinical and Health SciencesUniversity of South Australia&SA PathologyAdelaideSAAustralia Institute of Clinical Medicine Faculty of MedicineUniversity of OsloOsloNorway Department of Medical Genetics Oslo University HospitalOsloNorway Griffith Institute for Drug Discovery Griffith UniversityNathanQLDAustralia QIMR Berghofer Medical Research Institute Statistics UnitBrisbaneQLDAustralia school of Public health The University of QueenslandBrisbaneQLDAustralia school of Nursing Queensland University of TechnologyBrisbaneQLDAustralia eResearch Research InfrastructureAcademic DivisionQueensland University of TechnologyBrisbaneQLDAustralia Department of Anatomical Pathology Royal Brisbane and Women’s HospitalHerstonQLDAustralia Faculty of Medicine The University of QueenslandBrisbaneQLDAustralia Royal Brisbane and Women’s hospital Cancer Care ServicesHerstonQLDAustralia Department of Otolaryngology Royal Brisbane and Women’s HospitalHerstonQLDAustralia Department of Oral and Maxillofacial surgery Royal Brisbane and Women’s HospitalHerstonQLDAustralia

Oral cancer (OC) is the most common form of head and neck cancer. Despite the high incidence and unfavourable patient outcomes, currently, there are no biomarkers for the early detection of OC. This study aims to discover, develop, and validate a novel saliva-based microRNA signature for early diagnosis and prediction of OC risk in oral potentially malignant disorders (OPMD).The Cancer Genome Atlas (TCGA) miRNA sequencing data and small RNA sequencing data of saliva samples were used to discover differentially expressed miRNAs. Identified miRNAs were validated in saliva samples of OC (n=50), OPMD (n=52), and controls(n=60) using quantitative real-time PCR. Eight differentially expressed miRNAs (miR-7-5p, miR-10b-5p, miR-182-5p, miR-215-5p,miR-431-5p, miR-486-3p, miR-3614-5p, and miR-4707-3p) were identified in the discovery phase and were validated. The efficiency of our eight-miRNA signature to discriminate OC and controls was:area under curve (AUC):0.954, sensitivity:86%, specificity:90%,positive predictive value (PPV):87.8%and negative predictive value (NPV):88.5%whereas between OC and OPMD was:AUC:0.911,sensitivity:90%, specificity:82.7%, PPV:74.2%and NPV:89.6%. We have developed a risk probability score to predict the presence or risk of OC in OPMD patients. We established a salivary miRNA signature that can aid in diagnosing and predicting OC,revolutionising the management of patients with OPMD. Together, our results shed new light on the management of OC by salivary miRNAs to the clinical utility of using miRNAs derived from saliva samples.

关键词： specificity salivary diagnosis

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Energy Design and Optimization of Greenhouse by Natural Convection

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Fluid Dynamics & Materials Processing 2024年第8期20卷 1903-1913页

作者： h.Benzzine h.Labrim Aouatif saad Y.Achour D.Zejli R.El Bouayadi Advanced systems Engineering Laboratory(AsELab) National School of Applied SciencesIbn Tofail UniversityKenitraMorocco

This study addresses the pressing need for energy-efficient greenhouse management by focusing on the innovative application of natural *** primary objective of this study is to evaluate various ventilation strategies to enhance energy efficiency and optimize crop production in agricultural *** advanced numerical simulation tools,the study conducts a comprehensive assessment of natural ventilation’s effectiveness under real-world *** results underscore the crucial role of the stack effect and strategic window positioning in greenhouse cooling,providing valuable insights for greenhouse *** findings shed light on the significant benefits of optimized ventilation and also offer practical implications for improving greenhouse design,ensuring sustainable and efficient agricultural *** study demonstrated energy savings in cooling from November to April,with a maximum saving of 680 kWh in March,indicating the effectiveness of strategically positioning windows to leverage the stack *** approach enhances plant growth and reduces the need for costly cooling systems,thereby improving overall energy efficiency and lowering operational expenses.

关键词： Energy greenhouse cooling natural ventilation simulation temperature

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Connections Between Induction Generator Effect and Open-loop Modal Proximity to Cause sub-synchronous Oscillations in series-compensated Power system

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CsEE Journal of Power and Energy systems 2024年第3期10卷 963-973页

作者： Wenjuan Du Yang Wang h.F.Wang Tao Zhao Tim Littler College of Electrical Engineering Sichuan UniversityChengduChina school of Electrical Engineering the Queen’s University of BelfastBelfastUK IEEE

The induction generator effect(IGE)and the openloop modal proximity(OLMP)are two different reasons why subsynchronous oscillations(ssOs)in a series-compensated power system(sCPs)may *** IGE attributes the growing ssOs to negative resistance,while the OLMP explains the ssO mechanism from the standpoint of modal *** this paper,we investigate the connections between the IGE and the OLMP through equivalent RLC circuit and open-loop modal *** investigation is conducted for two types of seriescompensated power systems where either a synchronous generator or a DFIG is connected at the sending *** investigation reveals the conditions,in which the IGE and the OLMP may jointly cause the growing ssOs,i.e.,both the IGE and the OLMP can explain why the growing ssOs ***,the investigation indicates that the IGE and the OLMP may be totally irrelevant and lead to growing ssOs *** implies that it is possible that in a sCPs,the growing ssOs are only due to the IGE,and the OLMP is non-existent,and vice ***,when the growing ssOs occurs in a sCPs,examination based on both the IGE and the OLMP should be carefully conducted in order to find if the oscillatory instability is due to the IGE,or the OLMP,or both of them.

关键词： Induction generator effect open-loop modal proximity series compensation sub-synchronous oscillations

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Astrocytic calcium waves:unveiling their roles in sleep and arousal modulation

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Neural Regeneration Research 2024年第5期19卷 984-987页

作者： Erxi Wu Dan Qi Damir Nizamutdinov Jason h.huang Department of Neurosurgery and Neuroscience Institute Baylor Scott&White HealthTempleTXUSA Texas A&M University school of Medicine TempleTXUSA Texas A&M University school of Pharmacy College StationTXUSA LIVEsTRONG Cancer Institutes and Department of Oncology Dell Medical Schoolthe University of Texas at AustinAustinTXUSA Department of Neurosurgery Baylor College of MedicineTempleTXUSA

Neuron-astrocyte interactions are vital for the brain’s *** astrocyte activities is crucial for comprehending the complex neural network,particularly the population-level functions of neurons in different cortical states and associated behaviors in *** on animal sleep and wakefulness have revealed distinct cortical synchrony patterns between ***,outnumbering neurons by nearly fivefold,support and regulate neuronal and synaptic *** research on astrocyte activation during cortical state transitions has emphasized the influence of norepinephrine as a neurotransmitter and calcium waves as key components of ion channel *** summary focuses on a few recent studies investigating astrocyte-neuron interactions in mouse models during sleep,wakefulness,and arousal levels,exploring the involvement of noradrenaline signaling,ion channels,and glutamatergic signaling in different cortical *** findings highlight the significant impact of astrocytes on large-scale neuronal networks,influencing brain activity and *** astrocytic signaling pathways shows promise for treating sleep disorders and arousal *** research is needed to understand astrocytic calcium signaling in different brain regions and its implications for dysregulated brain states,requiring future human studies to comprehensively investigate neuron-astrocyte interactions and pave the way for therapeutic interventions in sleep-and arousal-related disorders.

关键词： arousal astrocyte calcium waves locus coeruleus neuron-astrocyte interaction norepinephrine sleep wakefulness

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Male genitourinary schistosomiasis-related symptoms among long-term Western African migrants in spain: a prospective population-based screening study

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贫困所致传染病(英文) 2024年第2期13卷 1-10页

作者： Roure sílvia Vallès Xavier Pérez-Quílez Olga López-Mu?oz Israel Chamorro Anna Abad Elena Valerio Lluís soldevila Laura Espa?a sergio hegazy Alaa h. A. Fernández-Rivas Gema Gorriz Ester herena Dolores Oliveira Mário Miralles Maria Carme Conde Carmen Montero-Alia Juan José Fernández-Pedregal Elia Miranda-sánchez Jose Llibre Josep M. Isnard Mar Bonet Josep Maria Estrada Oriol Prat Núria Clotet Bonaventura The schisto-stop study group International health Program (PROsICs) Barcelona North Metropolitan Territorial Directorate for Infectious Diseases Catalan Institute for Health CAP La Salut Passatge dels Encants S/N Badalona Spain Fundació Lluita contra les Infeccions c/Can Ruti s/n Badalona Spain Infectious Diseases Department Hospital Universitari Germans Trias i Pujol c/Can Ruti s/n Badalona Spain Germans Trias i Pujol Research Institute c/Can Ruti s/n Badalona Spain Microbiology Department Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Department of Genetics and Microbiology Universitat Autònoma de Barcelona c/Can Ruti s/n Badalona Spain Faculty of Medicine University of Asyut El Fateh Assiut Governorate Egypt Department of Urology Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Canovelles Primary health Care Unit Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Indústria Canovelles Spain Primary health Care Unit Mataró-(Rocafonda-Palau) Barcelona North Metropolitan Health Directorate Catalan Institute for Health Camí Ral el Ravalet Mataró Barcelona Spain Barcelona North Metropolitan Primary Care Directorate Catalan Institute for Health Ctra. de Barcelona Sabadell Barcelona Spain Directorate for Innovation and Interdisciplinary Cooperation Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain IrsiCaixa-AIDs Research Institute Hospital Universitari Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Infectious Diseases Directorate Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain

Background schistosomiasis is highly endemic in sub-saharan Africa and frequently imported to Europe. Male urogenital manifestations are often neglected. We aimed to ascertain the prevalence of genitourinary clinical signs and symptoms among long-term African migrants in a non-endemic European country using a serology *** We carried out a prospective, community-based cross-sectional study of adult male migrants from sub-saharan Africa living in *** serology tests and microscopic urine examinations were carried out, and clinical data were obtained from an electronic medical record search and a structured *** We included 388 adult males, mean age 43.5 years [standard Deviation (sD) = 12.0, range: 18–76]. The median time since migration to the European Union was 17 [Interquartile range (IQR): 11–21] years. The most frequent country of origin was senegal (N = 179, 46.1%). Of the 338, 147 (37.6%) tested positive forschistosoma. Parasite eggs were present in the urine of only 1.3%. Nine genitourinary clinical items were significantly associated with positiveschistosoma serology results: pelvic pain (45.2%;OR = 1.57, 95%CI: 1.0–2.4), pain on ejaculation (14.5%;OR = 1.85, 95%CI: 1.0–3.5), dyspareunia (12.4%;OR = 2.45, 95%CI: 1.2–5.2), erectile dysfunction (9.5%;OR = 3.10, 95%CI: 1.3–7.6), self-reported episodes of infertility (32.1%;OR = 1.69, 95%CI: 1.0–2.8), haematuria (55.2%;OR = 2.37, 95%CI: 1.5–3.6), dysuria (52.1%;OR = 2.01, 95%CI: 1.3–3.1), undiagnosed syndromic sTIs (5.4%), and orchitis (20.7%;OR = 1.81, 95%CI: 1.0–3.1). Clinical signs tended to *** Urogenital clinical signs and symptoms are prevalent among male African long-term migrants with a positiveschistosoma serology results. Genital involvement can be frequent even among those with long periods of non-residence in their sub-saharan African countries of origin. Further research is needed to develop diagnostic tools and validate therapeutic approaches to

关键词： schistosomiasis Chronic schistosomiasis Urogenital schistosomiasis Male genital schistosomiasis Longterm migrant

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Exploring Variability in sea Level at a Tide Gauge station through Control Charts

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Journal of Environmental & Earth sciences 2024年第1期6卷 11-18页

作者： h.Bâkiİz Independent scholar Boca Raton Florida33496USA

Monitoring temporal changes in sea level is important in assessing coastal *** level anomalies at a tide gauge station,if kinematically conceived,include systematic variations such as trend,acceleration,periodic oscillations,and random *** them,the non-stationary nature of the random sea level variations of known or unknown origin at coastal regions has been long recognized by the sea level *** study proposes the analyses of subgroups of random residual statistics of a rigorously formulated kinematic model solution of tide gauge variations using X-bar and s control *** approach is demonstrated using Key West,Florida tide gauge *** mean and standard errors of 5-year-long subgroups of the residuals revealed that sea level changes at this location have been progressively intensifying from 1913 to the *** oscillations in sea level at this locality may be attributed partly to the thermal expansion of seawater with increasing temperatures causing larger buoyancy-related sea level fluctuations as well as the intensification of atmospheric events including wind patterns and the impact of changes in inverted barometer effects that will alter coastal risk assessments for the future.

关键词： Climate change sea level variance X-bar s control charts

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