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scalable and automated CRIsPR-based strain engineering using droplet microfluidics

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Microsystems & Nanoengineering 2022年第2期8卷 227-236页

作者： Kosuke Iwai Maren Wehrs Megan Garber Jess sustarich Lauren Washburn Zachary Costello Peter W.Kim David ando William R.Gaillard Nathan J.Hillson Paul D.Adams Aindrila Mukhopadhyay Hector Garcia Martin Anup K.singh Technology Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA Biotechnology and Bioengineering Department Sandia National LaboratoriesLivermoreCA 94550USA Biofuels and Bioproducts Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA Biological systems and Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Molecular Biophysics and Integrated Bioimaging Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Department of Bioengineering University of CaliforniaBerkeleyCA 94720USA BCAM Basque Center for Applied MathematicsBilbao 48009Spain

We present a droplet-based microfluidic system that enables CRIsPR-based gene editing and high-throughput screening on a *** microfluidic device contains a 10x10 element array,and each element contains sets of electrodes for two electric field-actuated operations:electrowetting for merging droplets to mix reagents and electroporation for *** device can perform up to 100 genetic modification reactions in parallel,providing a scalable platform for generating the large number of engineered strains required for the combinatorial optimization of genetic pathways and predictable *** demonstrate the system's capabilities through the CRIsPR-based engineering of two test cases:(1)disruption of the function of the enzyme galactokinase(galK)in£coli and(2)targeted engineering of the glutamine synthetase gene(glnA)and the blue-pigment synthetase gene(bpsA)to improve indigoidine production in£coli.

关键词： operations generating fluid

Azathioprine is essential following cyclosporine for patients with steroid-refractory ulcerative colitis

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World Journal of Gastroenterology 2015年第1期21卷 254-261页

作者： Nobuyuki Miyake Takafumi ando Kazuhiro Ishiguro Osamu Maeda Osamu Watanabe Yutaka Hirayama Keiko Maeda Kazuhiro Morise Masanobu Matsushita Kazuhiro Furukawa Kohei Funasaka Masanao Nakamura Ryoji Miyahara Naoki Ohmiya Hidemi Goto Department of Gastroenterology and Hepatology Nagoya University Graduate School of MedicineNagoya CityAichi 466-8550Japan Department of Gastroenterology Fujita Health University School of MedicineToyoakeAichi 470-1192Japan

AIM: To evaluate long-term prognosis following cyclosporine treatment by examining the rate of surgery avoidance among cyclosporine ***: We retrospectively reviewed clinical records for 29 patients diagnosed with severe steroid-refractory ulcerative colitis in our hospital from August 1997 to August 2008 and treated with cyclosporine by continuous intravenous *** patients were treated with intravenous corticosteroids for more than 5 d prior to cyclosporine *** was continued for up to 21 d under serum monitoring to maintain cyclosporine levels between 400 and 600 ng/*** activity was assessed before and after cyclosporine therapy using the clinical activity index score,with a reduction of ≥ 5 considered to indicate a *** responders,we defined cases not requiring surgery for more than 5 years as exhibiting long-term efficacy of *** considered to be possibly predictive of long-term efficacy of cyclosporine were sex,age,disease duration,clinical activity index score,C-reactive protein level,hemoglobin level,disease extent,endoscopic findings,and clinical ***: Cyclosporine was not discontinued due to side effects in any ***(65.5%) of 29 patients were considered responders.A statistically significant(P = 0.004) inverseas sociation wa s observed between an endoscopic finding of "mucosal bleeding" and responsive ***(9 males,6 females) of these 19 patients were followed for 5 years or more,of whom 9(60%) exhibited long-termefficacy of *** the 10 non-responders,9(90%) underwent surgery within 6 mo of cyclosporine *** of the following factors had a significant impact on the long-term efficacy of cyclosporine: sex,age,duration of disease,clinical activity index score,C-reactive protein level,hemoglobin level,extent of disease,endoscopic findings,or clinical *** contrast,a significant association was observed for maintenance therapy with azat

关键词： Ulcerative colitis Cyclosporine Maintenance therap

日本传统“诗吟”中单个反射声优选时延与自相关函数的关系(英文)

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华南理工大学学报（自然科学版） 2007年第B10期35卷 92-95页

作者：加藤浩介田中慎一郎川井敬二矢野隆安藤四一 Center for Advanced science and Innovation Osaka University. 2-1 Yamada-oka Suita Osaka 565-0871 Japan Faculty of Engineering Kumamoto University Japan Graduate school of science and Technology Kumamoto University Japan Professor Emeritus Kobe University Japan

研究在日本传统"诗吟"表演中,在干信号中加上适当时延的单个反射声是否更为有利.专业"诗吟"表演者嗓音中对典型的"节"的吟唱是该种表演艺术的一种独特的吟咏方式.对此及对"节"外内容的吟唱都在消音室中加以录音.文中通过成对比较法试验... 详细信息

研究在日本传统"诗吟"表演中,在干信号中加上适当时延的单个反射声是否更为有利.专业"诗吟"表演者嗓音中对典型的"节"的吟唱是该种表演艺术的一种独特的吟咏方式.对此及对"节"外内容的吟唱都在消音室中加以录音.文中通过成对比较法试验得出了主观优选的标度值.结果表明,在聆听对"节"的吟唱时,听者更喜欢在干信号中加上延时为10～30ms的单个反射声,而在聆听对"节"外内容的吟唱时,加上单个反射声与否对听者而言并无显著的差别.文中对此项发现从吟唱"节"的声源信号的自相关函数的包络特征上加以讨论.

关键词：诗吟节吟唱自相关主观优选

患弥漫性糖尿病性黄斑水肿患者行吲哚青绿辅助性内界膜剥离的玻璃体切割术后视神经萎缩

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世界核心医学期刊文摘（眼科学分册） 2005年第5期1卷 38-39页

作者： ando F. Yasui O. Hirose H. Ohba N. 秦雪娇 EyeCare Nagoya Meieki 3-13-15 Nakamura-ku 450-0002 Nagoya Japan

Purpose: To report anatomic and visual outcomes of vitrectomy and indocyanine green (ICG)-assisted peeling of the retinal internal limiting membrane (ILM) in the treatment of diffuse diabetic macular edema. Methods: In a retrospective in terventional case series, 15 eyes of 11 patients with refractory diffuse diabeti c macular edema underwent pars plana vitrectomy with removal of the ILM, which w as stained by intravitreal injection of ICG (0.1-0.2 ml of 0.5%ICG), performed by a single surgeon. The patients were followed up for 14-28 months (mean 20.5 months). The main outcome measures were assessment of macular edema by optical coherence tomography and determination of visual acuity and visual field. Result s: Intravitreal ICG visualized the ILM to facilitate complete removal of the str ucture. Qualitative assessment of optical coherence tomography images at the end of follow-up revealed that retinal thickness in the macula appeared nearly nor mal with or without reappearance of foveal pit in 11 of the 15 eyes (73.3%), de creased in 3 eyes (20.0%), and did not change in 1 eye (6.6%). Best-corrected visual acuity at the end of follow-up improved by 2 lines or more in 4 eyes (2 6.7%), virtually unchanged in 6 eyes (40.0%), and deteriorated by 2 lines or m ore in 5 eyes (26.7%). The mean logMAR visual acuity was 0.680 (approximately 1 2/60) preoperatively and 0.812 (approximately 9/60) postoperatively, the differe nce being not statistically significant (paired t-test, P=0.445). seven (46.7% ) of the 15 eyes developed optic nerve atrophy that occurred gradually within 6 months after surgery and caused irreversible peripheral visual field defect pred ominantly affecting the nasal field. Conclusion: Intravitreal application of ICG is beneficial in uneventful ILM peeling to help resolution of diffuse diabetic macular edema, but it may potentially damage the optic nerve fibers and lead to unfavorable visual outcomes.

关键词：内界膜剥离玻璃体切割术视神经萎缩吲哚青绿玻璃体腔弥漫性黄斑水肿睫状体平坦部黄斑中心凹视功能

胰腺导管腺癌动脉侵犯的确诊：CT的最佳诊断标准是什么？

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国际医学放射学杂志 2023年第4期 491-491页

作者： Y.Noda N.Mizuno N.Kawai T.ando M.Kawaguchi s.Nagata 肖诗蕾

摘要目的研究确定胰腺导管腺癌（PDAC）动脉侵犯的诊断效能和观察者间变异性，并确定最佳CT成像标准。方法回顾性收集128例术前接受CT增强扫描的PDAC病人（男

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转甲状腺素蛋白ser50Ile和Tyr114Cys型淀粉样变性患者的肌肉淀粉样血管病

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世界核心医学期刊文摘（神经病学分册） 2005年第5期1卷 24-24页

作者： Yamashita T. ando Y. Katsuragi s. 江山 Department of Diagnostic Medicine Graduate School of Medical Sciences Kumamoto University 1- 1- 1 Honjo Kumamoto 860- 0811 Japan Dr.

Among patients with familial amyloid polyneuropathy (FAP), those with transth yretin Val30Met mainly show distally predominant weakness and atrophy, whereas s ome FAP patients, including those with transthyretin ser50Ile and Tyr114Cys, sho w muscle weakness and atrophy that is dominant proximally, simulating myopathy. To clarify the cause of proximally dominant muscular atrophy in patients with FA P transthyretin ser50Ile and Tyr114Cys, we investigated the distinctive features of muscle specimens of patients with FAP, 3 of who had Val30Met, 2 ser50Ile, an d 2 Tyr114Cys transthyretin. All specimens showed transthyretin amyloid around b lood vessels and perimysium, and neurogenic denervation patterns. The amount of amyloid around the vessels was much greater in patients with FAP ser50Ile and Ty r114Cys than in Val30Met patients. Muscular amyloid angiopathy may contribute to motor nerve injury that, in turn, may lead to amyotropic changes in patients wi th FAP ser50Ile and Tyr114Cys.

关键词：淀粉样血管病 ser50Ile Tyr114Cys 转甲状腺素蛋白淀粉样变性肌萎缩近端肌无力远端肌淀粉状蛋白神经源性

日本苦苣菜sonchus oleraceus L.的化学成分研究

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齐齐哈尔大学学报（自然科学版） 2007年第1期23卷 1-3页

作者：白玉华安政张维冰齐齐哈尔大学化学与化学工程学院黑龙江齐齐哈尔161006 日本新潟大学工学部

将新采摘的日本苦苣菜(sonchus oleraceus L.)粉碎后用甲醇浸泡,浸出液依次用正己烷、乙酸乙酯和氯仿萃取,制得相应的提取物。乙酸乙酯提取物被进一步采用柱层析及高效液相色谱分离,得到三种化合物。利用红外光谱、1H-NMR、13C-NMR方法... 详细信息

将新采摘的日本苦苣菜(sonchus oleraceus L.)粉碎后用甲醇浸泡,浸出液依次用正己烷、乙酸乙酯和氯仿萃取,制得相应的提取物。乙酸乙酯提取物被进一步采用柱层析及高效液相色谱分离,得到三种化合物。利用红外光谱、1H-NMR、13C-NMR方法对得到的化合物进行结构表征,证实其分别为β-谷甾醇、脱镁叶绿酸甲酯(Phaeophorbide-a methyl ester),和10-羟基脱镁叶绿酸甲酯Methyl(10s)-hydroxypheophorbide;其含量分别占乙酸乙酯提取物的0.0234%,0.115%和0.030%。

关键词：苦苣菜化学成分结果表征

应用卵泡刺激素试验预测体外受精的低反应状态

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世界核心医学期刊文摘（妇产科学分册） 2005年第8期1卷 62-62页

作者： Iwase A. ando H. Kuno K. Mizutani s. 王伟华 Dr.Department of Obstetrics Nagoya Univ. Grad. Sch. of Medicine 65 Tsurumai-cho Showa-ku Nagoya 466-8550Japan

OBJECTIVE: Optimized ovarian stimulation protocols are required for the success of in vitro fertilization (IVF). The purpose of this study was to estimate whether the ovarian reserve test using exogenous follicle-stimulating hormone (FsH) could predict ovarian response in ***: This was a prospective observational study of 110 patients who underwent their first IVF cycle. The FsH test was administered as 150 IU of urinary FsH daily from day 3 to day 6 of the menstrual cycle preceding the IVF cycle for evaluation of the plasma estradiol level. Outcomes of IVF, including ovarian response, were analyzed. REsULTs: A negative correlation was observed between the duration of stimulation and the result of the FsH test (r = -.238, P = .014) and between the dose of FsH per retrieved mature oocyte (metaphase II oocyte) and the result of the FsH test (r = -.308, P sults showed that the result of the FsH test was significantly lower in poor responders defined by FsH of 400 IU/metaphase II oocyte or greater (207 ±149 compared with 293 ±174 pg/mL, P =.007). CONCLUsION: The FsH test can be a useful tool for determining the conditions of individualized clinical management plans and optimizing stimulation protocols in IVF.

关键词：体外受精卵泡刺激素反应状态成熟卵母细胞卵巢储备功能促排卵方案血浆雌二醇刺激时间分裂中期评估应用

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来源异位胰腺的胃腺癌

世界核心医学期刊文摘（胃肠病学分册） 2005年第11期1卷 58-58页

作者： Matsuki M. Gouda Y. ando T. s.Kuriyama 李宏宇 Third Department of Internal Medicine Kagawa University School of Medicine 1750- 1 Ikenobe Miki-cho Kita-gun Kagawa 761- 0793Japan

We report here in a rare case of malignant transformation of aberrant pancreas in the stomach, associated with gastric outlet obstruction. A 58-year-old woman was admitted to our hospital, complaining of vomiting. Although barium meal examination and gastric endoscopy showed marked pyloric stenosis, the biopsy specimen obtained from the stenotic site revealed regenerative mucosa without malignancy. Abdominal computed tomography and endoscopic ultrasono-graphy (EUs) also could not detect the cause of the obstruction. Histopathological examination of the surgically resected specimen showed the malignant transformation of aberrant pancreas in the stomach. It should be noted that one of the causes of gastric outlet obstruction may be aberrant pancreas or its malignant transformation.

关键词：胃腺癌异位胰腺幽门梗阻超声内镜非恶性肿瘤病理学检查罕见病例幽门狭窄活检标本检查法