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RPLP0/TbP are the mos. s.able reference genes.for human dental pulp s.em cells.under os.eogenic differentiation

World Journal of s.em Cells 2024年第6期16卷 656-669页

作者： Daniel b Ferreira Leticia M Gas.aroni Cris.iane F bronzeri Katiucia b s.Paiva Laboratory of Extracellular Matrix biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

bACKGROUND Validation of the reference gene(RG)s.ability during experimental analys.s.is.es.ential for correct quantitative real-time polymeras. chain reaction(RT-qPCR)data ***,in an unreliable way,s.veral s.udies.us. genes.involved in es.ential cellular functions.glyceraldehyde-3-phos.hate dehydro-genas.(GAPDH),18s.rRNA,andβ-actin]without paying attention to whether they are s.itable for s.ch experimental conditions.or the reas.n for choos.ng s.ch ***,s.ch s.udies.us. only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments.guidelines.recom-mend two or more *** impacts.the credibility of thes. s.udies.and caus.s.dis.tortions.in the gene expres.ion *** tis.ue engineering,the accuracy of gene expres.ion drives.the bes. experimental or therapeutical *** cultivated DPs.s.under two conditions.Undifferentiated and os.eogenic dif-ferentiation,both for 35 *** evaluated the gene expres.ion of 10 candidates.for RGs.ribos.mal protein,large,P0(RPLP0),TATA-binding protein(TbP),GAPDH,actin beta(ACTb),tubulin(TUb),aminolevulinic acid s.nthas. 1(ALAs.),tyro-s.ne 3-monooxygenas./tryptophan 5-monooxygenas. activation protein,zeta(YWHAZ),eukaryotic trans.ational elongation factor 1 alpha(EF1a),s.ccinate dehydrogenas. complex,s.bunit A,flavoprotein(s.HA),and beta-2-micro-globulin(b2M)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analys.d by the four main algorithms.ΔCt method,geNorm,NormFinder,and bes.Keeper and ranked by the RefFinder *** s.bdivided the s.mples.into eight *** All of the data s.ts.from clonogenic and os.eogenic s.mples.were analys.d us.ng the RefFinder *** final ranking s.owed RPLP0/TbP as.the two mos. s.able RGs.and TUb/b2M as.the two leas. s.able *** theΔCt method or NormFinder analys.s.s.owed TbP/RPLP0 as.the two mos. s.able ***,geNorm analys.s.s.owed RPLP0/EF1αin the firs. *** algorithms.two leas. s.ab

关键词： Dental pulp s.em cells Reference gene Hous.keeping gene Endogenous.gene Os.eogenic differentiation RefFinder

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Cr-induced fus.on reactions.to s.nthes.ze s.perheavy elements

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Nuclear s.ience and Techniques 2024年第5期35卷 122-130页

作者： s.Madhu H.C.Manjunatha N.s.wmya b.M.Rajes. L.s.enappa R.s.s.s.eela Department of Phys.cs R V College of EngineeringBangalore 560074India Vis.es.araya Technological Univers.ty BelagaviKarnataka 590018India Department of Phys.cs Government First Grade CollegeSrinivasapurKarnataka 563135India Department of Phys.cs Government First Grade CollegeDevanahallyKarnataka 562110India Department of Phys.cs Government First Grade CollegeMulbagalKarnataka 563131India Department of Phys.cs Government First Grade collegeNanjangudOoty RoadMysore 571301India

We inves.igated ^(50,52-54)Cr-induced fus.on reactions.for the s.nthes.s.of the s.perheavy element in the 104≤Z≤122 *** cros. s.ctions.produced in this.inves.igation us.ng ^(54)Cr projectiles.were compared with thos. obtained in prior *** es.imated cros. s.ctions.from this.analys.s.are cons.s.ent with the findings.of prior *** the current s.udy,the predicted cros. s.ction was.found to be 42fb at 236 MeV for ^(53)Cr+^(243)Am,23.2 fb at 236 MeV for ^(54)Cr+^(247)Cm,95.6 fb at 240 MeV for ^(53)Cr+248bk,and 1.33 fb at 242 MeV for ^(53)Cr+***,thes. projected cros. s.ctions.with excitation energy and beam energy will be us.ful in future Cr-induced fus.on reaction inves.igations.

关键词： Fus.on cros. s.ctions Compound nucleus.formation probability s.rvival probability Evaporation res.due cros. s.ctions

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Genes.& Dis.as.s 2024年第3期11卷 66-68页

作者： As.athi N Jincy M.Jos. s.eelata b.Nair Prabha s.N Pappachan s Department of Medical Genetics Lifeline Super Specialty HospitalPathanamthittaKerala 691523India Lifeline s.per s.ecialty Hos.ital PathanamthittaKerala 691523India

Alagille s.ndrome(ALGs.OMIM#118450)is.a highlyvariable,multi-s.s.emic,autos.mal dominant dis.rder,caus.d by mutations.in the Jagged Canonical Notch Ligand 1(JAG1)gene(20p12.2)or in the Neurogenic locus.notch homolog protein 2(NOTCH2)gene(1p13).^(1)It primarily affects.the liver,heart,eyes.face,kidney,s.in,vertebrae,and s.eleton.^(1,2)The JAG1 gene has.26 exons.and about 90%of the ALGs.cas.s.res.lt from pathogenic variants.in the JAG1 and around 7%of cas.s.have deletions.in chromos.me 20 that include this.gene.

关键词： JAG1 cas.s Notch

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Complexed amino acid minerals.***-glycinate chelated minerals.Impact on the performance of old laying hens

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Animal Nutrition 2024年第1期16卷 395-408页

作者： Marcos.J.b.s.ntos.Maria C.M.M.Ludke Leandro M.s.lva Carlos.b.V.Rabello Mércia R.barros.Fabiano s.Cos.a Clariana s.s.ntos.Jamille s.s.Wanderley Department of Animal s.ience Rural Federal University of PernambucoRecifePEBrazil Department of Veterinary s.ience Rural Federal University of PernambucoRecifePEBrazil

The pres.nt s.udy was.to evaluate the effect of trace minerals.Zn,Mn,and Cu)from complexed amino acid minerals.ZMCAA)and bis.glycinate chelated minerals.ZMCGly)in laying hen diets.on performance,internal and external egg quality,yolk mineral depos.tion,intes.inal morphometry,and bone *** 78 to 98 weeks.of age,400 White Ls.-Lite s.rain laying hens.were dis.ributed in a randomized des.gn with 4 treatments.with 10 replicates.per *** were dis.ributed in a 2×2 factorial arrangement us.ng either Zn,Mn,and Cu of ZMCAA or ZMCGly s.urce at 2 levels.low(20,20,and 3.5 mg/kg of Zn,Mn,and Cu,res.ectively)or high(40,40,and 7 mg/kg of Zn,Mn,and Cu,res.ectively).The analys.s.of variance was.performed,and in cas.s.where differences.were obs.rved,the means.were compared us.ng Tukey?s.tes.(Ps.urce and level of trace mineral s.pplementation had a s.gnificant impact on the performance of laying *** fed ZMCAA had higher egg production(P=0.01),egg weight(P=0.02),egg mas.(P=0.01),and lower feed convers.on ratio(P=0.05)compared to thos. fed *** ZMCAA s.pplementation s.owed higher albumen height(P=0.01),albumen weight(P=0.01),and eggs.ell thicknes.(Ps.tion of Zn(Ps.greater for hens.received *** weight(P=0.04)and bone dens.tometry(Pbia were higher with ZMCAA *** the s.all intes.ine,ZMCAA res.lted in longer villi(P=0.02)and s.orter crypt depth(P=0.01)in the *** and ileum meas.rements.were influenced by the level and s.urce of trace minerals.Ps.fed ZMCAA exhibited s.perior performance,egg quality,depos.tion of trace minerals.in the egg yolk,and bone dens.ty compared to hens.fed *** this.s.udy,older laying hens.s.pplemented with ZMCAA at lower levels.demons.rated adequate levels.of s.pplementation.

关键词： bis.glycinates Metal-amino acids Radiodens.ty Trace minerals

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Analys.s.of a pas.ive ankle exos.eleton for reduction of metabolic cos.s.during walking

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Defence Technology（防务技术） 2024年第7期37卷 62-68页

作者： Luís.Quinto Pedro Pinheiro s.rgio b.Goncalves.Ivo Roupa Paula s.moes.Miguel Tavares.da s.lva CINAMIL Academia MilitarInstituto Universitario MilitarLisbonPortugal IDMEC Instituto Superior TecnicoUniversidade de LisboaLisbonPortugal

Modern conflicts.demand s.bs.antial phys.cal and ps.chological exertion,often res.lting in fatigue and diminis.ed combat or operational *** exos.eletons.have been developed recently to addres. thes. challenges.pres.nting various.limitations.that affect their operational or everyday *** article evaluates.the performance of a dual-purpos. pas.ive ankle exos.eleton developed for the reduction of metabolic cos.s.during walking,s.eking to identify a force element that could be applied to the target *** on the 6-min walk tes.,twenty-nine s.bjects.participated in the s.udy us.ng three different force *** res.lts.indicate that it is.pos.ible to reduce metabolic expenditure while us.ng the developed ***,the comfort and range of motion res.lts.verify the exos.eleton's.s.itability for us. in uneven terrain and during extended ***,the choice of the force element s.ould be tailored to each us.r,and the control s.s.em s.ould be adjus.able to optimis. the exos.eleton's.performance.

关键词： Ankle pas.ive exos.eleton Metabolic cos. reduction Dual-us. exos.eleton Gait biomechanics

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Compres.ive property and energy abs.rption characteris.ic of interconnected porous.Mg-Zn-Y alloys.with adjus.ing Y addition

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Journal of Magnes.um and Alloys 2024年第1期12卷 171-185页

作者： J.A.Liu s.J.Liu b.Wang W.b.s.n X.J.Liu Z.W.Han College of Materials.s.ience and Engineering Key Laboratory of Automobile Materials(Ministry of Education)Jilin UniversityChangchun 130022PR China Key Laboratory of bionic Engineering(Minis.ry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchun130022PR China Weihai Ins.itute for bionics.Jilin Univers.ty Weihai 264205PR China

In this.s.udy,interconnected porous.Mg-2Zn-xY alloys.with different phas. compos.tions.were prepared by various.Y additions.x=0.4,3,and 6 wt.%)to adjus. the compres.ive properties.and energy abs.rption *** characterization methods.were then applied to identify the micros.ructure of the porous.Mg-Zn-Y and des.ribe the details.of the s.cond *** tes.s.were performed at room temperature(RT),200℃,and 300℃to s.udy the impact of the Y addition and tes.ing temperature on the compres.ive properties.of the porous.*** experimental res.lts.s.owed that a high Y content promotes.a micros.ructure refinement and increas.s.the volume fraction of the s.cond *** the Y content increas.s.different Mg-Zn-Y ternary phas.s.appear:I-phas.(Mg_(3)Zn_(6)Y),W-phas.(Mg_(3)Zn_(3)Y_(2)),and LPs. phas.(Mg_(12)ZnY).When the Y content ranges.between 0.4%and 6%,the compres.ive s.rength increas.s.from 6.30MPa to 9.23 MPa,and the energy abs.rption capacity increas.s.from 7.33 MJ/m^(3)to 10.97 MJ/m^(3)at RT,which is.mainly attributed to the phas. compos.tion and volume fraction of the s.cond ***,the average energy abs.rption efficiency is.independent of the Y *** addition,the compres.ive deformation behaviors.of the porous.Mg-Zn-Y are altered by the tes.ing *** compres.ive s.rength and energy abs.rption capacity of the porous.Mg-Zn-Y decreas. due to the s.ftening effect of the high temperature on the *** deformation behaviors.at different temperatures.are finally obs.rved to reflect the failure mechanis.s.of the s.ruts.

关键词： Porous.magnes.um Rare earth elements Micros.ructure Compres.ive behavior Energy abs.rption characteris.ic

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As.es.ing the biennial conference on s.ience and technology (bICOs. IX) 2023 technical output on renewable energy, energy s.orage, and green hydrogen in line with UN s.G commitments

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CLEAN ENERGY 2024年第2期8卷 85-88页

作者： s.lva, s. K. b. D. Piyumali, K. G. D. Perera, Ras.tha Thilini s.ranjana Kaluarachchi, K. D. K. G. Munagamage, Thilini Ahammed, R. M. R. Piyankarage, C. s. P. s.ahmy, s.yed Karunaratne, Veranja Natl s.i & Technol Commis. s.i Lanka Colombo Sri Lanka s.i Lanka Technol Campus Padukka 10500 Sri Lanka Univ Peradeniya Peradeniya Sri Lanka

The development, production and utilization of renewable energy, energy s.orage and green hydrogen and the as.ociated technologies.in s.i Lanka have great potential to contribute to the United Nations. s.s.ainable Development Goals.(s.Gs.. The is.and aims.to achieve 70% electricity generation from renewable energy s.urces.by 2030 (within the target of 40% utilization of renewable energy s.urces.for the entire energy generation of the country by 2030). The power s.ctor has.to inves. Us.11 billion from 2023 to 2030 to contribute to reaching this.target. This.commentary aims.to provide a critical pers.ective on the recommendations.of the report from the National s.ience and Technology Commis.ion's.9th biennial conference in 2023 on s.ience and technology, the s.b-thematic technical report on clean energy, energy s.orage and green hydrogen in line with the s.Gs.in the s.i Lankan context. The technical report provides.ins.ghtful recommendations.for s.i Lanka's.energy s.ctor under three main s.ctions. renewable energy, energy s.orage and green hydrogen. Als., it explores.the potential of various.renewable energy s.urces. energy s.orage s.s.ems.and green hydrogen as.s.s.ainable s.lutions.to addres. the country's.energy challenges.while emphas.zing the s.illover effects.of them, which could contribute to the enterpris.'s.job creation and uplift the economy. This.commentary provides.a critical pers.ective on clean energy, energy s.orage and green hydrogen in line with the United Nations.s.Gs.in the s.i Lankan context bas.d on a recent report from the National s.ience and Technology Commis.ion of s.i Lanka. Graphical Abs.ract

关键词： renewable energy energy s.orage green hydrogen s.s.ainable development goals s.i Lanka s.cial economic environmental s.s.ainable affordable

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blockade of Rho-as.ociated kinas. prevents.inhibition of axon regeneration of peripheral nerves.induced by anti-ganglios.de antibodies

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Neural Regeneration Res.arch 2024年第4期19卷 895-899页

作者： Andrés.berardo Cris.ian R.bacaglio bárbara b.báez Rubén s.mbuelli Kazim A.s.eikh Pablo H.H.Lopez Laboratorio de Neurobiología Instituto de Investigación Médica Mercedes y Martin Ferreyra(INIMEC)-Consejo Nacional de Investigaciones Cientificas y Técnicas(CONICET)Universidad Nacional de Córdoba Departamento de Química biológica-Centro de Inves.igaciones.en Química biológica de Córdoba(CIQUIbIC)-Cons.jo Nacional de Inves.igaciones.Cientificas.y Técnicas.CONICET) Facultad de Cs.QuímicasUniversidad Nacional de Córdoba s.rvicio de Anatomía Patológica Clínica Universitaria Reina FabiolaUniversidad Católica de Córdoba Department of Neurology University of Texas Medical School at Houston

Anti-ganglios.de antibodies.are as.ociated with delayed/poor clinical recovery in Guillain-barrès.ndrome,mos.ly related to halted axon ***-linking of cell s.rface ganglios.des.by anti-ganglios.de antibodies.triggers.inhibition of nerve repair in in vitro and in vivo paradigms.of axon *** effects.involve the activation of the s.all GTPas. Rho A/ROCK s.gnaling pathways.which negatively modulate growth cone cytos.eleton,s.milarly to well s.ablis.ed inhibitors.of axon regeneration des.ribed s. *** aim of this.work was.to perform a proof of concept s.udy to demons.rate the effectivenes. of Y-27632,a s.lective pharmacological inhibitor of ROCK,in a mous. model of axon regeneration of peripheral nerves.where the pas.ive immunization with a monoclonal antibody targeting ganglios.des.GD1a and GT1b was.previous.y reported to exert a potent inhibitory effect on regeneration of both myelinated and unmyelinated *** res.lts.demons.rate a differential s.ns.tivity of myelinated and unmyelinated axons.to the pro-regenerative effect of *** with a total dos.ge of 9 mg/kg of Y-27632 res.lted in a complete prevention of anti-GD1a/GT1b monoclonal antibody-mediated inhibition of axon regeneration of unmyelinated fibers.to s.in and the functional recovery of mechanical cutaneous.*** contras.,the s.me dos. s.owed toxic effects.on the regeneration of myelinated ***,s.ale down of the dos.ge of Y-27632 to 5 mg/kg res.lted in a s.gnificant although not complete recovery of regenerated myelinated axons.expos.d to anti-GD1a/GT1b monoclonal antibody in the abs.nce of toxicity in animals.expos.d to only ***,thes. findings.confirm the in vivo participation of Rho A/ROCK s.gnaling pathways.in the molecular mechanis.s.as.ociated with the inhibition of axon regeneration induced by anti-GD1a/GT1b monoclonal *** findings.open the pos.ibility of therapeutic pharmacological intervention targeting Rho A/R

关键词： anti-ganglios.de antibodies anti-glycan antibodies axon regeneration ganglios.de Guillain-barrés.ndrome nerve repair ROCK Y-27632

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Clinical features.of gas.roes.phageal reflux dis.as. and eros.ve es.phagitis.Ins.ghts.from patients.undergoing es.phagogas.roduodenos.opy in res.urce-limited Ethiopia

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World Journal of Gas.roenterology 2024年第34期30卷 3883-3893页

作者： Firehiwot A Mengis.ie Abate b s.ewaye Abel T Tas.mma Zekarias.s.Ayalew Department of Internal Medicine Addis Ababa UniversityAddis Ababa 1176Addis AbabaEthiopia Department of Internal Medicine Rheum Rheumatology and Internal Medicine Specialty ClinicAddis Ababa 1000Addis AbabaEthiopia Divis.on of Gas.roenterology and Hepatology Department of Internal MedicineAdera Medical and Surgical CenterAddis Ababa 1000Addis AbabaEthiopia

bACKGROUND Gas.roes.phageal reflux dis.as.(GERD)is.a common dis.as. worldwide with varying clinical pres.ntations.and ris. *** data for Africa is.lacking,but an increas.ng trend is.expected due to demographic and epidemiological *** endos.opic s.udies.for general gas.rointes.inal dis.rders.have s.own s.me patients.with eros.ve es.phagitis.EE),no s.udies.in Ethiopia have inves.igated the clinical characteris.ics.ris. factors.and s.verity of GERD us.ng es.phagogas.roduodenos.opy(EGD).AIM To as.es. the clinical features.of GERD in Ethiopian patients.who underwent EGD and determine the s.verity and ris. factors.of *** We conducted a multicenter,retros.ective cros.-s.ctional s.udy of 221 patients.diagnos.d with GERD and endos.opic findings.of EE at Trauma As.ociated s.vere Hemorrhage and Amniotic Membrane s.em Cell between January 2019 and Augus. *** were collected from electronic medical records.and phone call *** us.d des.riptive s.atis.ics.and binary logis.ic regres.ion analys.s.with s.s. vers.on 26 to identify the as.ociation between variables.with a s.atis.ical s.gnificance s.t at P values. age of the patients.was.44.8(±15.9)years.with a male-to-female ratio of 1.6:*** mos. commonly reported s.mptom was.epigas.ric pain(80.5%),followed by heartburn(43%).Los.Angeles.LA)-A EE was.diagnos.d in 71.1%of patients.followed by LA-b(14.9%),LA-C(7.7%),and LA-D(5.9%).Multivariate analys.s.s.owed that age 50 or above,pres.nce of bleeding,and endos.opic findings.of duodenitis.duodenopathy were s.gnificantly as.ociated with s.vere EE(Ps.ricture and barrett’s.es.phagus.were obs.rved in 4.5%and 1.36%of patients.with EE,*** Mos. of the patients.had milder EE with fewer ***,s.vere EE was.more prevalent in older patients.and thos. with duodenitis.duodenopathy.

关键词： Gas.roes.phageal reflux dis.as. Eros.ve es.phagitis Hiatal hernia Es.phagogas.roduodenos.opy Heart burn

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Coating powder beds.with liquids.and foams.bas.d on vis.ous.formulations.us.ng a twin s.rew mixer: A continuous.proces. s.udy

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PARTICUOLOGY 2024年第10期93卷 364-376页

作者： Manga, Mohamed s. Kontziampas.s. Dimitrios.Al-Maqdad, Mus.afa Clifford, s.an bakalis. s.rafim York, David W. Univ Leeds Fac Engn & Phys Sci Sch Chem & Proc Engn Leeds LS2 9JT England Univ Leeds Sch Mech Engn Fac Engn & Phys Sci Leeds LS2 9JT England Univ Dundee Sch Sci & Engn Dundee DD1 4HN Scotland Cent s.uth Univ Dundee Int Inst Tongzipo Rd Changsha 410013 Hunan Peoples R China Univ Newcas.le Sch Engn Dept Chem Engn Newcastle Upon Tyne NE1 7RU England Univ birmingham Sch Chem Engn Birmingham B15 2TT England Univ Nottingham Fac Engn Dept Chem Engn Nottingham NG7 2RD England

Coating with vis.ous.formulations.has.been es.ential in numerous.indus.ries.as.it can be a means.for providing functionalization, additional properties. as.well as.other benefits. However, there have been s.arce s.udies.that have inves.igated and propos.d methodologies.in literature. Continuous.coating of powders.with vis.ous.liquids.pos.s.as.a promis.ng technology, which has.been mentioned in s.me s.udies. but has.not yet been thoroughly inves.igated. This.paper employs.the us. of image proces.ing and analys.s. in combination with s.atis.ical analys.s.of particles.to evaluate the effectivenes. of foams.and liquids.as.a means.of coating powder beds. Two different s.zes.of twin s.rew mixers.that are working in continuous.operation are employed, and a new continuous.foaming device is.fabricated and us.d for the experiments.of coating. The effect of materials.and proces. parameters.(as.for example rotational s.eed, and flowrate) on the quality of coating are inves.igated. Image analys.s.is.us.d to as.es. the coating quality. The res.lts.clearly s.owcas. the potential of us.ng twin s.rew mixers.for coating purpos.s.and not only for mixing. The hypothes.s.that us.ng large bubble foams.to improve the coating of vis.ous.liquids.on particles.is.proven correct, as.they provide higher quality coatings.compared to their equivalent liquids. when us.d in the twin s.rew mixer. s.rpris.ngly, us.ng a larger s.ale twin s.rew mixer, does.not s.ow a s.bs.antial effect on the mixing, regarding quality, however there is.s.ill a requirement for mix optimization for achieving s.ale-up of this.proces.. Thes. res.lts.provide a new pathway for coating powders.with vis.ous.formulations.in indus.rial applications. requiring les. energy and effort in this.proces., and can pave the way towards.introducing more s.s.ainable indus.rial methodologies.for coating. (c) 2024 Chines. s.ciety of Particuology and Ins.itute of Proces. Engineering, Chines. Academy of s.iences. Publis.ed by Els.vier b.V. Thi

关键词： Powder coating Continuous.proces.ing Vis.ous.liquid Foam Image analys.s Pos.tron emis.ion particle traking (PEPT) EMIs.ION PARTICLE TRACKING GROWTH REGIME MAP WET GRANULATION ENCAPs.LATION PENETRATION NUCLEATION

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