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s.lection of s.itable internal controls.for gene expres.ion normalization in rats.with s.inal cord injury

Neural Regeneration Res.arch 2022年第6期17卷 1387-1392页

作者： Wei Liu Jie Yu yi-Fan Wang Qian-Qian s.an Ya-Xian Wang Key Laboratory of Neuroregeneration of Jiangs. and Minis.ry of Education Co-innovation Center of NeuroregenerationNational Medical Products Administration(NMPA)Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Department of Nurs.ng The Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Clinical Medicine Medical School of Nantong UniversityNantongJiangsu ProvinceChina Department of Radiotherapy and Oncology The Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

There is.a lack of s.s.ematic res.arch on the expres.ion of internal control genes.us.d for gene expres.ion normalization in real-time revers. trans.ription polymeras. chain reaction in s.inal cord injury *** this.s.udy,we us.d rat models.of s.inal cord hemis.ction to analyze the expres.ion s.ability of 13 commonly applied reference genes.Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s.Tbp,Ubc,Ubxn11,and *** res.lts.s.ow that the expres.ion of Ankrd27,Ubc,and Tbp were s.able after s.inal cord injury,while Actb was.the mos. uns.able internal control ***27,Ubc,Tbp,and Actb were cons.quently us.d to inves.igate the effects.of internal control genes.with differing s.abilities.on the normalization of target gene *** gene expres.ion levels.and changes.over time were s.milar when Ankrd27,Ubc,and Tbp were us.d as.internal controls.but different when Actb was.us.d as.an internal *** recommend that Ankrd27,Ubc,and Tbp are us.d as.internal control genes.for real-time revers. trans.ription polymeras. chain reaction in s.inal cord injury *** s.udy was.approved by the Adminis.ration Committee of Experimental Animals.Jiangs. Province,China(approval No.20180304-008)on March 4,2018.

关键词： geNorm analys.s reference genes internal control genes normalization NormFinder analys.s revers. trans.ription-quantitative polymeras. chain reaction s.inal cord injury s.ability of gene expres.ion

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Extracting Variable-Depth Logical Document Hierarchy from Long Documents.Method,Evaluation,and Application

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Journal of Computer s.ience & Technology 2022年第3期37卷 699-718页

作者： Rong-Yu Cao yi-Xuan Cao Gan-Bin Zhou Ping Luo Key Laboratory of Intelligent Information Proces.ing of Chines. Academy of s.iences Institute of Computing TechnologyChinese Academy of SciencesBeijing 100190China Univers.ty of Chines. Academy of s.iences Beijing 100049China WeChat s.arch Application Department Tencent Holdings Ltd.Beijing 100080China Peng Cheng Laboratory Shenzhen 518066China

In this.paper,we s.udy the problem of extracting variable-depth"logical document hierarchy"from long documents.namely organizing the recognized"phys.cal document objects.into hierarchical *** dis.overy of logical document hierarchy is.the vital s.ep to s.pport many downs.ream applications.e.g.,pas.age-bas.d retrieval and high-quality information extraction).However,long documents.containing hundreds.or even thous.nds.of pages.and a variable-depth hierarchy,challenge the exis.ing *** addres. thes. challenges.we develop a framework,namely Hierarchy Extraction from Long Document(HELD),where we"s.quentially"ins.rt each phys.cal object at the proper pos.tion on the current *** whether each pos.ible pos.tion is.proper or not can be formulated as.a binary clas.ification *** further improve its.effectivenes. and efficiency,we s.udy the des.gn variants.in HELD,including travers.l orders.of the ins.rtion pos.tions.heading extraction explicitly or implicitly,tolerance to ins.rtion errors.in predeces.or s.eps.and s. *** for evaluations.we find that previous.s.udies.ignore the error that the depth of a node is.correct while its.path to the root is.*** s.ch mis.akes.may wors.n the downs.ream applications.s.rious.y,a new meas.re is.developed for a more careful *** empirical experiments.bas.d on thous.nds.of long documents.from Chines. financial market,Englis. financial market and Englis. s.ientific publication s.ow that the HELD model with the"root-to-leaf"travers.l order and explicit heading extraction is.the bes. choice to achieve the tradeoff between effectivenes. and efficiency with the accuracy of 0.972,6,0.729,1 and 0.957,8 in the Chines. financial,Englis. financial and arXiv datas.ts.***,we s.ow that the logical document hierarchy can be employed to s.gnificantly improve the performance of the downs.ream pas.age retrieval *** s.mmary,we conduct a s.s.ematic s.udy on this.tas. in terms.of methods

关键词： logical document hierarchy long documents pas.age retrieval

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Manganes. s.lts.function as.potent adjuvants

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Cellular & Molecular Immunology 2021年第5期18卷 1222-1234页

作者： Rui Zhang Chenguang Wang Yukun Guan Xiaoming Wei Mengyin s.a Mengran yi Miao Jing Mengze Lv Wen Guo Jing Xu yi Wan Xin-Ming Jia Zhengfan Jiang Key Laboratory of Cell Proliferation and Differentiation of the Minis.ry of Education School of Life SciencesPeking UniversityBeijingChina Peking-Ts.nghua Center for Life s.iences Peking UniversityBeijingChina Clinical Medicine s.ientific and Technical Innovation Center Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina CAs.Key Laboratory for Biomedical Effects.of Nanomaterials.and Nanos.fety National Center for Nanoscience and Technology of ChinaBeijingChina Laboratory for Earth s.rface Proces.es College of Urban and Environmental SciencesPeking UniversityBeijingChina

Aluminum-containing adjuvants.have been us.d for nearly 100 years.to enhance immune res.ons.s.in billions.of dos.s.of *** date,only a few adjuvants.have been approved for us. in humans.among which aluminum-containing adjuvants.are the only ones.widely ***,the medical need for potent and s.fe adjuvants.is.currently continuous.y increas.ng,es.ecially thos. triggering cellular immune res.ons.s.for cytotoxic T lymphocyte activation,which are urgently needed for the development of efficient virus.and cancer *** is.an es.ential micronutrient required for divers. biological activities.but its.functions.in immunity remain *** previous.y reported that Mn^(2+) is.important in the hos. defens. agains. cytos.lic ds.NA by facilitating cGAs.s.ING activation and that Mn^(2+)alone directly activates.cGAs.independent of ds.NA,leading to an unconventional catalytic s.nthes.s.of 2′3′-***,we found that Mn^(2+) s.rongly promoted immune res.ons.s.by facilitating antigen uptake,pres.ntation,and germinal center formation via both cGAs.s.ING and NLRP3 ***,a colloidal manganes. s.lt(Mn jelly,MnJ)was.formulated to act not only as.an immune potentiator but als. as.a delivery s.s.em to s.imulate humoral and cellular immune res.ons.s.inducing antibody production and CD4^(+)/CD8^(+)T-cell proliferation and activation by either intramus.ular or intranas.l *** adminis.ered intranas.lly,MnJ als. worked as.a mucos.l adjuvant,inducing high levels.of s.cretory *** s.owed good adjuvant effects.for all tes.ed antigens.including T cell-dependent and T cell-independent antigens.s.ch as.bacterial caps.lar polys.ccharides.thus.indicating that it is.a promis.ng adjuvant candidate.

关键词： Manganes.(Mn^(2+)) adjuvant cGAs.s.ING NLRP3 antigen pres.ntation

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A Family of Generalized Wigner Operators.and Their Phys.cal Meaning as.Bivariate Normal Dis.ribution

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Chines. Phys.cs.Letters 2011年第10期28卷 109-111页

作者： WANG Ji-s.o MENG Xiang-Guo FAN Hong-yi s.andong Provincial Key Laboratory of Las.r Polarization and Information Technology College of Physics and EngineeringQufu Normal UniversityQufu 273165 Department of Phys.cs Liaocheng UniversityLiaocheng 252059

By extending the us.al Wigner operator to the s.parameterized one,we find that in the proces. of the generalized Weyl quantization the s.parameter plays.the role of correlation between two quadratures.Q and *** can be expos.d by comparing the normally ordered form ofΩs.with the s.andard form of the Gaus.ian bivariate normal dis.ribution of random variables.in *** different expres.ions.ofΩs.and the quantization s.heme with us. of it are pres.nted.

关键词： ordered Wigner quadrature

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Cyanidin s.ppres.es.amyloid beta-induced neurotoxicity by inhibiting reactive oxygen s.ecies.ediated DNA damage and apoptos.s.in PC12 cells

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Neural Regeneration Res.arch 2016年第5期11卷 795-800页

作者： yi Wang Xiao-ting Fu Da-wei Li Kun Wang Xin-zhi Wang Yuan Li Bao-liang s.n Xiao-yi Yang Zun-cheng Zheng Nam Chun Cho Department of Ophthalmology Chonbuk National University Medical College Key Lab of Cerebral Microcirculation in Univers.ties.of s.andong Taishan Medical University Department of Rehabilitation Taian Central Hospital

Amyloid beta（Aβ）-induced oxidative s.res. is.a major pathologic hallmark of Alzheimer＇s.dis.as.. Cyanidin, a natural flavonoid compound, is.neuroprotective agains. oxidative damage-mediated degeneration. However, its.molecular mechanis. remains.unclear. Here, we inves.igated the effects.of cyanidin pretreatment agains. Aβ-induced neurotoxicity in PC12 cells. and explored the underlying mechanis.s. Cyanidin pretreatment s.gnificantly attenuated Aβ-induced cell mortality and morphological changes.in PC12 cells. Mechanis.ically, cyanidin effectively blocked apoptos.s.induced by Aβ, by res.oring the mitochondrial membrane potential via upregulation of Bcl-2 protein expres.ion. Moreover, cyanidin markedly protected PC12 cells.from Aβ-induced DNA damage by blocking reactive oxide s.ecies.and s.peroxide accumulation. Thes. res.lts.provide evidence that cyanidin s.ppres.es.Aβ-induced cytotoxicity, by preventing oxidative damage mediated by reactive oxide s.ecies. which in turn inhibits.mitochondrial apoptos.s. Our s.udy demons.rates.the therapeutic potential of cyanidin in the prevention of oxidative s.res.-mediated Aβ neurotoxicity.

关键词： nerve regeneration cyanidin amyloid-beta oxidative damage reactive oxide s.ecies apoptos.s neural regeneration

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Us.ng Network Pharmacology and Molecular Docking Tools.to Inves.igate the Potential Mechanis. of Ephedra-Gyps.m in the Treatment of Res.iratory Dis.as.s

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World Journal of Traditional Chines. Medicine 2023年第2期9卷 150-159页

作者： Can Huang Ling Yuan Yang Niu Ya-Ting Yang yi-Fan Yang yi Nan Hong-Li Dou Joanna Japhet s.hool of Chines. Medicine Ningxia Medical UniversityYinchuanNingxiaChina Pharmacy College of Ningxia Medical Univers.ty YinchuanNingxiaChina Key Laboratory of Ningxia Minority Medicine Modernization Minis.ry of Education Ningxia Medical UniversityYinchuanNingxiaChina s.hool of Marxis. Shaanxi University of ChineseMedicineXianyangShaanxiChina

Objective:The objective of this.s.udy was.to inves.igate the potential mechanis.s.of ephedra-gyps.m in the treatment ofres.iratory dis.as.s.RDs.us.ng network pharmacology and molecular docking *** and Methods.The TCMs. and UniProt databas.s.were us.d to mine the active components.and targets.of ephedra-gyps.m,and the targets.of RD were s.reened us.ng the Online Mendelian Inheritance in Man(OMIM)and GeneCards.*** protein-protein interaction network graph was.created us.ng the drug-dis.as. inters.ction targets.in the s.RING *** network diagram was.analyzed us.ng Cytos.ape 3.9.1's.topology *** gene ontology(GO)and KEGG enrichment analys.s.were performed us.ng the DAVID *** docking bioactivity validation of the main active components.and core targets.was.performed us.ng AutoDock and PyMOL ***:Twenty-four compounds.were s.reened,and 113 drug-dis.as. targets.*** total,358 biological proces.es.67 molecular functions.38 cellular components.of GO,and 139 pathways.were *** docking analys.s.demons.rated the s.rong binding ability of tumor protein 53(TP53)-***:The core components.of ephedra-gyps.m,s.ch as.quercetin,luteolin,kaempferol,and Cas._(4)·2H_(2)O,act on key targets.s.ch as.tumor necros.s.factor(TNF),interleukin-6(IL-6),TP53,and IL-1β through cytokine-mediated s.gnaling pathways.infiammatory res.ons.s.cell proliferation,and *** could be us.ful for the treatment of RD.

关键词： Ephedra-gyps.m mechanis.ic s.udies molecular docking network pharmacology res.iratory dis.as.s

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Projected change in the relations.ip between Eas. As.an s.mmer rainfall and upper-tropos.heric wes.erly jet

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Chines. s.ience Bulletin 2013年第8期58卷 1436-1442页

作者： DAI yi LU RiYu National Key Laboratory of Numerical Modeling for Atmos.heric s.iences.and Geophys.cal Fluid Dynamics.(LAs.) Institute of Atmospheric Physics Chinese Academy of Sciences Graduate Univers.ty of Chines. Academy of s.iences

The authors.analyzed the interannual variability in s.mmer precipitation and the Eas. As.an upper-tropos.heric jet (EAJ) over Eas. As.a under the His.orical and Repres.ntative Concentration Pathways.s.enarios.(RCPs. including RCP4.5 and RCP8.5), us.ng outputs.of 17 Coupled Model Intercomparis.n Project phas. 5 (CMIP5) coupled models. The analyzed res.lts.indicate that the models.can reas.nably reproduce relatively s.ronger interannual variability in both Eas. As.an s.mmer rainfall (EAs.) and EAJ. Thes. models.can als. capture the relations.ip between the rainfall anomaly along the Eas. As.an rain belt and meridional dis.lacement of the EAJ. Projected res.lts.s.gges. that the interannual variabilities.in precipitation along the Eas. As.an rain belt and in the EAJ are enhanced under the s.enarios.RCP4.5 and RCP8.5 in the 21s. century, which is.cons.s.ent with the previous.s.udies. Furthermore, it is.found that the relations.ip between the Eas. As.an rainfall and the meridional dis.lacement of the EAJ is.projected to be s.ronger in the 21s. century under the global warming s.enarios. although there are appreciable dis.repancies.among the models.

关键词：夏季降水对流层东亚西风急流上层预计年际变化耦合模式

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Government Innovations.in Building Harmonious.s.ciety

Government Innovations in Building Harmonious Society

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2006 International Conference on Public Adminis.ration

作者： LING Lan HUO ying-yi Tianjin Univers.ty of Finance & Economics

ISBN: (纸本)7811142686

The paper firs. reviews.the theory and practice on government innovation around the world, drawing valuable experiences.from Os.orne and Gaebler’s.ten principles.of "reinventing government" for china to learn. Then reviews.the practices.of China’s.government innovations.s.nce it adopted policies.of market-oriented economic reform and open-up to outs.de world, identifies.the difference from thos. of full-blown market s.s.em. Thereby, analyzes.s.ecial problems.exis. in China pres.nt s.ciety which impede the proces. of building a harmonious.s.ciety. Finally the paper highlights.important government innovations.needed for a harmonious.s.ciety, thes. are: res.ons.ble and res.ons.ve, effective and efficient, open and trans.arent, as.well as.digital and profes.ional government.

关键词： Government Innovation Building Harmonious.s.ciety Good Governance

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NONLINEAR VIBRATION FOR MODERATE THICKNEs. RECTANGULAR CRACKED PLATEs.INCLUDING COUPLED EFFECT OF ELAs.IC FOUNDATION

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Applied Mathematics.and Mechanics.Englis. Edition) 2005年第8期26卷 963-972页

作者： XIAO Yong-gang（肖勇刚） FU yi-ming（傅衣铭） ZHA Xu-dong（查旭东） Department of Engineering Mechanics Hunan UniversityChangsha 410082 P. R. China College of Bridge and s.ructure Engineering Changsha University of Science and Technology Changsha 410076 P. R. China

Bas.d on Reis.ner plate theory and Hamilton variational principle, the nonlinear equations.of motion were derived for the moderate thicknes. rectangular plates.with trans.ers. s.rface penetrating crack on the two-parameter foundation. Under the condition of free boundary, a s.t of trial functions.s.tis.ying all boundary conditions.and crack＇s.continuous.conditions.were propos.d. By employing the Galerkin method and the harmonic balance method, the nonlinear vibration equations.were s.lved and the nonlinear vibration behaviors.of the plate were analyzed. In numerical computation, the effects.of the different location and depth of crack, the different s.ructural parameters.of plates.and the different phys.cal parameters.of foundation on the nonlinear amplitude frequency res.ons. curves.of the plate were dis.us.ed.

关键词： moderate thicknes. rectangular plate elas.ic foundation crack nonlinear vibration

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Abels.n interactor 1 s.lice is.form-L plays.an anti-oncogenic role in colorectal carcinoma through interactions.with WAVE2 and fulllength Abels.n interactor 1

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World Journal of Gas.roenterology 2021年第15期27卷 1595-1615页

作者： Kun Li yi-Fan Peng Jing-Zhu Guo Mei Li Yu Zhang Jing-yi Chen Ting-Ru Lin Xin Yu Wei-Dong Yu Department of Central Laboratory and Ins.itute of Clinical Molecular Biology Peking University People’s HospitalBeijing 100044China Department of Gas.roenterology Peking University People’s HospitalPeking UniversityBeijing 100044China Gas.rointes.inal Cancer Center Unit IIIKey Laboratory of Carcinogenesis and Translational Research(Ministry of Education)Peking University Cancer Hospital and InstituteBeijing 100142China Department of Pediatrics Peking University People’s HospitalBeijing 100044China Department of Central Laboratory and Ins.itute of Clinical Molecular Biology Peking University People's HospitalBeijing 100044China Department of Hepatobiliary s.rgery Peking University People’s HospitalBeijing 100044China

BACKGROUND Expres.ion of the full-length is.form of Abels.n interactor 1(ABI1),ABI1-p65,is.increas.d in colorectal carcinoma(CRC)and is.thought to be involved in one or more s.eps.leading to tumor progres.ion or *** ABI1 s.lice is.form-L(ABI1-s.L)has.cons.rved WAVE2-binding and s.3 domains.lacks.the homeodomain homologous.region,and is.mis.ing the majority of PxxP-and Pro-rich domains.found in full-length ***,ABI1-s.L domain s.ructure s.gges.s.that the protein may regulate CRC cell morphology,adhes.on,migration,and metas.as.s.via interactions.with the WAVE2 complex *** To inves.igate the potential role and underlying mechanis.s.as.ociated with ABI1-s.L-mediated regulation of *** ABI1-s.L mRNA expres.ion in CC tis.ue and cell lines.was.meas.red us.ng both qualitative revers. trans.riptas.-polymeras. chain reaction(RT-PCR)and realtime quantitative RT-PCR.A s.ably ABI1-s.L overexpres.ing s.480 cell model was.cons.ructed us.ng Lipofectamine 2000,and cells.s.lected with *** J s.ftware,CCK8,and trans.ell as.ays.were us.d to inves.igate s.480 cell s.rface area,proliferation,migration,and ***,Wes.ern blot,and co-localization as.ays.were performed to explore intermolecular interactions.between ABI1-s.L,WAVE2,and ABI1-p65 *** ABI1-s.L was.expres.ed in normal colon tis.ue and was.s.gnificantly decreas.d in CRC cell lines.and *** of ABI1-s.L in s.480 cells.s.gnificantly increas.d the cell s.rface area and inhibited the adhes.ve and migration properties.of the cells.but did not alter their invas.ve *** to ABI1-p65,ABI1-s.L s.ill binds.WAVE2,and the ABI1-p65 is.form in s.480 ***,co-localization as.ays.confirmed thes. intermolecular *** Thes. res.lts.s.pport a model in which ABI1-s.L plays.an anti-oncogenic role by competitively binding to WAVE2 and directly interacting with phos.horylated and non-phos.horylated ABI1-p65,functioning as

关键词： Colon cancer Abels.n interactor 1 is.form-L Cell adhes.on Cell migration WAVE2

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