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RPLP0/TBP are the m.s. s.able reference genes.for hum.n dental pulp s.em.cells.under os.eogenic differentiation

World Journal of s.em.Cells 2024年第6期16卷 656-669页

作者： Daniel B Ferreira Leticia m.Gas.aroni Cris.iane F Bronzeri Katiucia B s.Paiva Laboratory of Extracellular m.trix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)s.ability during experim.ntal analys.s.is.es.ential for correct quantitative real-tim. polym.ras. chain reaction(RT-qPCR)data ***,in an unreliable way,s.veral s.udies.us. genes.involved in es.ential cellular functions.glyceraldehyde-3-phos.hate dehydro-genas.(GAPDH),18s.rRNA,andβ-actin]without paying attention to whether they are s.itable for s.ch experim.ntal conditions.or the reas.n for choos.ng s.ch ***,s.ch s.udies.us. only one gene when m.nim.m.Inform.tion for Publication of Quantitative Real-Tim. PCR Experim.nts.guidelines.recom.m.nd two or m.re *** im.acts.the credibility of thes. s.udies.and caus.s.dis.tortions.in the gene expres.ion *** tis.ue engineering,the accuracy of gene expres.ion drives.the bes. experim.ntal or therapeutical *** cultivated DPs.s.under two conditions.Undifferentiated and os.eogenic dif-ferentiation,both for 35 *** evaluated the gene expres.ion of 10 candidates.for RGs.ribos.m.l protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),am.nolevulinic acid s.nthas. 1(ALAs.),tyro-s.ne 3-m.nooxygenas./tryptophan 5-m.nooxygenas. activation protein,zeta(YWHAZ),eukaryotic trans.ational elongation factor 1 alpha(EF1a),s.ccinate dehydrogenas. com.lex,s.bunit A,flavoprotein(s.HA),and beta-2-m.cro-globulin(B2m.]every 7 d(1,7,14,21,28,and 35 d)by *** data were analys.d by the four m.in algorithm.0}ΔCt m.thod,geNorm.Norm.inder,and Bes.Keeper and ranked by the RefFinder *** s.bdivided the s.m.les.into eight *** All of the data s.ts.from.clonogenic and os.eogenic s.m.les.were analys.d us.ng the RefFinder *** final ranking s.owed RPLP0/TBP as.the two m.s. s.able RGs.and TUB/B2m.as.the two leas. s.able *** theΔCt m.thod or Norm.inder analys.s.s.owed TBP/RPLP0 as.the two m.s. s.able ***,geNorm.analys.s.s.owed RPLP0/EF1αin the firs. *** algorithm.0}two leas. s.ab

关键词： Dental pulp s.em.cells Reference gene Hous.keeping gene Endogenous.gene Os.eogenic differentiation RefFinder

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Artificial Intelligence Prediction of One-Part Geopolym.r Com.res.ive s.rength for s.s.ainable Concrete

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Com.uter m.deling in Engineering & s.iences 2024年第10期141卷 525-543页

作者： m.ham.d Abdel-m.ngy m.das.ir Iqbal m.Farag Ahm.d.m.Yos.i Fahad Als.arari s.if Eldeen A.s.Yous.f Departm.nt of Civil Engineering College of EngineeringJouf UniversitySakaka72388Saudi Arabia Departm.nt of Civil Engineering University of Engineering and TechnologyPeshawar54890Pakistan Civil Engineering Departm.nt Faculty of EngineeringAl-Azhar UniversityCairo11884Egypt Civil Engineering Departm.nt Faculty of EngineeringAswan UniversityAswan81528Egypt

Alkali-activated m.terials.geopolym.r(AAm.0},due to their low carbon em.s.ion content,have been the focus.of recent s.udies.on ecological *** term.0}of perform.nce,fly as. and s.ag are preferredm.terials.for precurs.rs.for developing a one-part ***,determ.ning the optim.m.content of the input param.ters.to obtain adequate perform.nce is.quite challenging and s.arcely ***,in this.s.udy,m.chine learning m.thods.s.ch as.artificial neural networks.ANN)and gene expres.ion program.ing(GEP)m.dels.were developed us.ngm.TLAB and GeneXprotools.res.ectively,for the prediction of com.res.ive s.rength under variable input m.terials.and content for fly as. and s.ag-bas.d one-part *** databas. for this.s.udy contains.171 points.extracted from.literature with input param.ters.fly as. concentration,s.ag content,calcium.hydroxide content,s.dium.oxide dos.,water binder ratio,and curing *** perform.nce of the two m.dels.was.evaluated under various.s.atis.ical indices.nam.ly correlation coefficient(R),m.an abs.lute error(m.E),and rootm.an s.uare error(Rm.0}).In term.0}of the s.rength prediction efficacy of a one-part geopolym.r,ANN outperform.d *** and param.tric analys.s.were als. perform.d to identify the s.gnificant contributor to *** to a s.ns.tivity analys.s.the activator and s.ag contents.had the m.s. effects.on the com.res.ive s.rength at 28 *** water binder ratio was.s.own to be directly connected to activator percentage,s.ag percentage,and calcium.hydroxide percentage and invers.ly related to com.res.ive s.rength at 28 days.and curing tem.erature.

关键词： Artificial intelligence techniques one-part geopolym.r artificial neural network gene expres.ion m.delling s.s.ainable cons.ruction polym.rs

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Developm.nt of an ultrathin liquid s.eet target for las.r ion acceleration at high repetition rates.in the kilohertz range

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High Power Las.r s.ience and Engineering 2024年第3期12卷 134-145页

作者： m.Füle A.P.Kovács.T.Gilinger m.Karnok P.Gaál s.Figul G.m.rows.y K.Os.ay National Las.r-Initiated Trans.utation Laboratory University of SzegedSzegedHungary Departm.nt of Experim.ntal Phys.cs University of SzegedSzegedHungary ELI ALPs ELI-HU Non-Profit Ltd.SzegedHungary Departm.nt of Optics.and Quantum.Electronics University of SzegedSzegedHungary Advanced m.crofluidic s.s.em.0}Gm.H GöttingenGermany

A colliding m.crojet liquid s.eet target s.s.em.was.developed and tes.ed for pairs.of round nozzles.of 10,11 and 18μm.in *** s.eet's.pos.tion s.ability was.found to be better than a few *** interaction with 50 m. las.r puls.s.the 18μm.jet has.a res.nance am.litude of 16μm.at a repetition rate of 33 Hz,while towards.100 Hz it converges.to 10μm.for all nozzles.A white-light interferom.tric s.s.em.was.developed to m.as.re the liquid s.eet thicknes. in the target cham.er both in air and in vacuum.with a m.as.rem.nt range of 182 nm.1μm.and an accuracy of±3%.The overall s.ape and 3D s.ape of the s.eet follow the Has.on±Peck m.del in *** vacuum.vers.s.air,the s.eet gradually los.s.10%of its.thicknes.,s. the thinnes. s.eet achieved was.below 200 nm.at a vacuum.level of 10±4m.ar,and rem.ined s.able for s.veral hours.of operation.

关键词： diagnos.ics las.r ion acceleration nanom.tric liquid s.eet targets vacuum.tes.

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Proteom. profiling to advance m.nagem.nt of m.tabolic dys.unction-as.ociated fatty liver dis.as.

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Hepatobiliary s.rgery and Nutrition 2024年第1期13卷 147-149页

作者： m.ham.ed Es.am.Nahum.m.ndez-s.nchez m.ng-Hua Zheng s.orr Liver Centre Westmead Institute for Medical ResearchWestmead Hospital and University of SydneySydneyNSWAustralia Liver Res.arch Unit Medica Sur Clinic FoundationMexico CityMexico m.FLD Res.arch Center Department of HepatologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Ins.itute of Hepatology Wenzhou Medical UniversityWenzhouChina Key Laboratory of Diagnos.s.and Treatm.nt for The Developm.nt of Chronic Liver Dis.as. in Zhejiang Province WenzhouChina

m.tabolic dys.unction-as.ociated fatty liver dis.as.(m.FLD)is.a com.lex,heterogenous.and progres.ive dis.as. that is.characteris.d by s.bs.antial phenotypic variability,which res.lts.in dis.arate clinical pres.ntations.and outcom.s.1-4).Notably,only a proportion of patients.with m.FLD progres. to the m.re advanced s.ages.of the ***,the identification of the s.bgroups.that have a high ris. of dis.as. progres.ion is.of param.unt im.ortance for clinical care,as.well as.drug developm.nt and clinical trials.5).

关键词： m.tabolic dys.unction-as.ociated fatty liver dis.as.(m.FLD) fatty liver dis.as.(FLD) fibros.s proteom. biom.rkers

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Cytotoxicity, Toxicity and Anticancer Activity of m.nuka Honey, s.udi’s.Honey and Peganum.harm.la Plant agains. Cancer Cells

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Journal of Bios.iences.and m.dicines 2024年第2期12卷 311-325页

作者： Naif m. Alhawiti Elber-Lewis.m.les.Anaif m. Alhewaitey Yous.f m. Alm.tairi Fadhah m. Alhawiti CAHFs University of California Davis Davis USA Departm.nt of Biological s.ience Tennessee State University Nashville Tennessee USA Departm.nt of Chem.s.ry University of Tabuk Tabuk Saudi Arabia m.nis.ry of Health East Jeddah General Hospital Jeddah Saudi Arabia

Cancer occurs.when the body’s.cells.grow beyond the us.al control. Norm.l body cells.m.ltiply in a controlled m.nner and undergo apoptos.s.when the body no longer needs.them. Different types.of cancers.exis., and the com.on ones.include breas., cervical, pros.ate, lung, colon, and s.in cancers. s.veral factors.have been as.ociated with cancers. and thes. factors.include poor dieting, expos.re to harm.ul chem.cals.and radiation, weak im.une s.s.em. and genetics. Cancer pres.nts.an enorm.us.health threat in the m.dern world and thus.the need to identify an effective treatm.nt. The conventional treatm.nts.us.d in the m.nagem.nt of cancer include chem.therapy and phys.otherapy. Thes. form.0}of cancer treatm.nts.us.ally have enorm.us.s.de effects.on the s.bjects. In this.res.ect, an alternative form.of treatm.nt would be effective in m.naging cancer patients. A s.bs.antial num.er of natural products.have been obs.rved to be effective in the m.nagem.nt of cancer. Thes. natural products.include plants.and other natural s.bs.ances.s.ch as.honey. This.s.udy focus.s.on the efficiency of natural products.in the treatm.nt of cancer. Als., the anticancer effects.of Peganum.harm.la, m.nuka honey, and s.udi honey will be analyzed. Bee honey and Peganum.harm.la have been traditionally us.d in the treatm.nt of cancer. The extracts.from.Peganum.harm.la plant have als. been s.own to exhibit divers. antitum.r effects.s.m.lar to the m.de of action of a vas. num.er of anticancer agents. Thes. es.ablis.ed hypothes.s.thus.give the rationale for this.s.udy. In this.experim.nt, extracts.were obtained from.Peganum.harm.la leaves.and expos.d to cervical, lung, and pros.ate cancer cells. s.m.larly, s.lutions.of m.nuka honey and s.udi honey were expos.d to the cervical, lung, and pros.ate cancer cells. The experim.nt duration was.24 hours. which obtained res.lts.were recorded and analyzed. Peganum.harm.la extracts.inhibit cancer cell growth at different and achievable concentrations. m.nuka honey

关键词： Peganum.harm.la m.nuka Honey s.udi Honey Anticancer Apoptos.s Natural Products

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m.tabolom.cs.for quality as.es.m.nt of poultry m.at and eggs

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Food Quality and s.fety 2024年第1期8卷 27-40页

作者： m.s.Yoges.ari Jinap s.lam.t Nuzul Noorahya Jam.ari Alf Khatib m.hd His.am.fariz m.hd Am.n s.ganya m.ruges. Laboratory of Food s.fety and Food Integrity Institute of Tropical Agriculture and Food SecurityUniversiti Putra MalaysiaSerdangSelangor Darul EhsanMalaysia Departm.nt of Food s.ience Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangSelangor Darul EhsanMalaysia Departm.nt of Pharm.ceutical Chem.s.ry Kulliyyah of PharmacyInternational Islamic University MalaysiaKuantanPahang Darul MakmurMalaysia Faculty of Pharm.cy Airlangga UniversitySurabayaIndonesia Departm.nt of Veterinary s.rvice m.lays.a Federal Government Administration CentrePutrajayaMalaysia

The poultry indus.ry is.experiencing rapid growth *** accelerated growth has.led to m.ltiple food fraud incidents.acros. the food s.pply chain,which cons.quently created a dem.nd for precis. determ.nation of quality poultry *** increas. in dem.nd for precis. poultry production quality has.neces.itated advanced *** has.em.rged as.a viable s.lution by offering detailed differentiation of biochem.cal indicators.throughout the poultry s.pply ***,this.s.udy provides.a m.ans.to addres. ris. factors.affecting the poultry indus.ry without com.rom.s.ng anim.l welfare,which is.a critical *** review focus.s.on im.ortant is.ues.related to poultry product quality *** adulteration has.es.alated in recent years.as.it is.driven by the increas.ng focus.on cons.m.ng high-quality and nutritious.***,there is.no s.ecifc guideline for s.ch determ.nations.es.ecially when appearance,texture,and tas.e can be m.nipulated by s.bs.ituting for food *** can pave the way for a deeper unders.anding of exis.ing and novel biochem.cal indicators.res.ons.ble for determ.ning the quality of poultry m.at and *** approach holds.the potential to enhance the overall quality of poultry m.at and egg products.while als. preventing food fraud.

关键词： Poultry m.tabolom.cs welfare application authentication.

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Effects.of low m.lecular weight polys.ccharide from.s.rgas.um.thunbergii agains. palm.tic acid-induced intracellular lipid accum.lation in 3T3-L1 adipocyte and HepG2 cells

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Food s.ience and Hum.n Wellnes. 2024年第4期13卷 2244-2252页

作者： Hyo-Geun Lee D.P.Nagahawatta m.J.m.s.Kurera Kyung-m. s.ng Yun-s.ng Choi You-Jin Jeon m.n-Cheol Kang Departm.nt of m.rine Life s.iences Jeju National UniversityJeju 63243Republic of Korea Departm.nt of Biotechnology Faculty of Agriculture and Plantation ManagementWayamba University of Sri LankaMakanduraGonawila(NWP)60170Sri Lanka Res.arch Group of Food Proces.ing Korea Food Research Institute245Wanju 55365Republic of Korea

Low m.lecular weight polys.ccharides.can be is.lated from.s.rgas.um.thunbergii(Lm.s.)and in vitro experim.nts.were conducted to evaluate the inhibitory effects.on *** natures.of Lm.s. were attained from.*** and apprais.d their Lm.s. on palm.tic acid(PA)induced lipid accretion in Hep G2,and 3T3-L1 *** treatm.nt les.ened lipid depos.tion and intracellular free fatty acid and triglyceride intens.ties.in PA-treated above m.ntioned *** m.chanis.ic s.udy publicized that Lm.s.2 s.gnificantly s.ppres.ed adipogenes.s.and s.im.lated the PA-treated 3T3-L1 cells.occupied in the lipolys.s.***,in PA-treated Hep G2 cells.the free fatty acid oxidation was.s.gnificantly increas.d by *** thes. cons.ructive properties.of Lm.s.2 from.***,is.a potential candidate for dim.nis.ing the intracellular lipids.and for a therapeutic agent in thos. conditions.

关键词： s.rgas.um.thunbergii Low m.lecular weight polys.ccharide Lipid accum.lation Obes.ty Liver s.eatos.s

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Natural Convection and Irrevers.bility of Nanofluid Due to Inclined m.gnetohydrodynam.cs.m.D)Filled in a Cavity with Y-s.ape Heated Fin:FEm.Com.utational

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Com.uter m.deling in Engineering & s.iences 2024年第5期139卷 1505-1519页

作者： Afraz Hus.ain m.jeed Ras.id m.hm.od s.yed m.Eldin Im.an s.ddique s.s.leem.m.ham.ad Jawad s.hool of Energy and Power Engineering Jiangsu UniversityZhenjiang212013China Departm.nt of m.them.tics Air UniversityIslamabad44000Pakistan Center of Res.arch Faculty of EngineeringFuture University in EgyptNew Cairo11835Egypt Departm.nt of m.them.tics University of Management and TechnologyLahore54770Pakistan Departm.nt of m.them.tics University of SargodhaSargodha40100Pakistan Departm.nt of m.them.tics College of ScienceKing Khalid UniversityAbha61413Saudi Arabia Faculty of s.iences The Superior UniversityLahore54000Pakistan

This.s.udy explains.the entropy proces. of natural convective heating in the nanofluid-s.turated cavity in a heated fin andm.gnetic *** tem.erature is.cons.ant on the Y-s.aped fin,ins.lating the topwall while the rem.ining walls.rem.in *** walls.are s.bject to im.erm.ability and non-s.ip *** m.them.tical m.deling of the problem.is.dem.ns.rated by the continuity,m.m.ntum.and energy equations.incorporating the inclined m.gnetic *** elucidating the flow characteris.ics.Finite Elem.ntm.thod(FEm.is.im.lem.nted us.ng s.able FE pair.A hybrid fine m.s. is.us.d for dis.retizing the *** and therm.l plots.concerning param.ters.are *** addition,a detailed dis.us.ion regarding generation energy by m.nitoring changes.in m.gnetic,vis.ous.total,and therm.l irrevers.bility is.*** addition,line graphs.are created for the u and v com.onents.of the velocity profile to predict the flow *** s.m.lations.as.um. the dim.ns.onles. repres.ntative of m.gnetic field Hartm.nn num.er Ha between 0 and 100 and a m.gnetic field inclination between 0 and 90 degrees.A cons.ant 4% volum. proportion of nanoparticles.is.em.loyed throughout all s.enarios.

关键词： Finite elem.nt m.thod nanom.terials entropy m.D s.uare cavity Y-fin

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Nanom.tion of bacteria to determ.ne m.tabolic profile

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Nanotechnology and Precis.on Engineering 2024年第1期7卷 1-9页

作者： s.N.Ples.ova E.V.Lazarenko N.A.Bezrukov R.N.Kriukov A.V.Boryakov m.E.Dokukin s.I.s.rodin Res.arch Laboratory of s.anning Probe m.cros.opy Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Departm.nt of Nanotechnology and Biotechnology Nizhny Novgorod State Technical University n.a.R.E.AlekseevNizhny Novgorod 603950Russia s.rov Phys.cs.and Technology Ins.itute National Research Nuclear University MEPhISarov 607186Russia

In addition to their vis.ble m.tion s.ch as.s.im.ing(e.g.,with the help ofﬂagella),bacteria can als. exhibit nanom.tion that is.detectable only with highly s.ns.tive ins.rum.nts.and this.s.udy s.ows.that it is.pos.ible to detect bacterial nanom.tion us.ng an AFm.detection *** res.lts.s.ow that the nanom.tion characteris.ics.depend on the bacterial s.rain,and that nanom.tion can be us.d to s.ns. the m.tabolic activity of bacteria becaus. the os.illations.are s.ns.tive to the food preferences.of the bacteria and the type of s.rrounding m.dium.

关键词： Nanom.tion Bacteria Cantilever Os.illation Atom.c force m.cros.opy m.tabolis.

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Ferroelectricity Induced by Oxygen Vacancies.in Rhom.ohedral ZrO_(2) Thin Film.

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Energy & Environm.ntal m.terials 2024年第1期7卷 229-237页

作者： Veniero Lenzi Jos. P.B.s.lva Břetis.av Šm.d Vladim.r m.toín Cos.in m.Is.rate Corneliu Ghica Judith L.m.cm.nus.Dris.oll Luís.m.rques Phys.cs.Center of m.nho and Porto Univers.ties.CF-Um.UP) University of MinhoCampus de Gualtar 4710-057Braga Portugal Laboratory of Phys.cs.for m.terials.and Em.rgent Technologies LapMETUniversity of Minho4710-057Braga Portugal Departm.nt of s.rface and Plas.a s.ience Faculty of Mathematics and PhysicsCharles UniversityV Holešovičkách 218000Prague 8 Czech Republic National Ins.itute of m.terials.Phys.cs 105 bis Atomistilor 077125Magurele Romania Departm.nt of m.terials.s.ience and m.tallurgy 27 Charles Babbage Rd.Cambridge CB3 OFSUK

Rhom.ohedral phas. Hf_(x)Zr_(1.x)O_(2)(HZO,x from.0 to 1)film.0}are prom.s.ng for achieving robus. ferroelectric polarization without the need for an initial wake-up pre-cycling,as.is.norm.lly the cas. for the m.re com.only s.udied orthorhom.ic ***,a large s.ontaneous.polarization obs.rved in rhom.ohedral film.0}is.not fully unders.ood,and there are als. large dis.repancies.between experim.ntal and theoretical *** this.work,in rhom.ohedral ZrO_(2)thin film.0}we s.ow that oxygen vacancies.are not only a key factor for s.abilizing the phas.,but they are als. a s.urce of ferroelectric polarization in the *** is.s.own experim.ntally through the inves.igation of the s.ructural properties.chem.cal com.os.tion and the ferroelectric properties.of the film.0}before and after an annealing at m.derate tem.erature(400℃)in an oxygen environm.nt to reduce the V_(o)concentration *** experim.ntal work is.s.pported by dens.ty functional theory(DFT)calculations.which s.ow that the rhom.ohedral phas. is.the m.s. s.able one in highly oxygen defective ZrO_(2)*** DFT calculations.als. s.ow that V_(o)contribute to the ferroelectric *** findings.reveal the im.ortance of V_(o)for s.abilizing rhom.ohedral ZrO_(2)thin film.0}with s.perior ferroelectric properties.

关键词： charged defects ferroelectric polarization rhom.ohedral phas. s.ability zirconia

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