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Immunohistochemical analysis of p53,cyclinD1,RB1,c-fos and N-ras gene expression in hepatocellular carcinoma in Iran

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World Journal of Gastroenterology 2007年第4期13卷 588-593页

作者： sJ Moghaddam EN Haghighi s samiee N shahid AR Keramati s Dadgar MR Zali Research Center for Gastroenterology and Liver Disease Shaheed Beheshti University of Medical Sciences and Academy of Medical Sciences Iran

AIM： TO study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma. METHODs： Paraffin-embedded tissue samples of 25 patients （18 males and 7 females） with hepatocellular carcinoma were collected from 22 pathology centers in Tehran during 2000-2001, and stained using immunohistochemistry method （avidin-biotin-peroxidase） for detection of p53, cyclinD1, RB1, c-los and N-ras proteins. REsULTs： six （24%）, 5 （20%）, 12 （48%） and 2 samples （8%） were positive for p53, cyclinDl, C-los and N-ras expression, respectively. Twenty-two （88%） samples had alterations in the （31 cell-cycle checkpoint protein expression （RBI or cyclinD1）. P53 positive samples showed a higher （9 times） risk of being positive for RBI protein than p53 negative samples. Loss of expression of RBI in association with p53 over-expression was observed in 4 （66.7%） of 6 samples. Loss of expression of RBI was seen in all cyclinD1 positive, 20 （90.9%） N-ras negative, and ii （50%） C-fos positive samples, respectively. CyclinD1 positive samples showed a higher （2.85 and 4.75 times） risk of being positive for c-los and N-ras expression than cyclinD1 negative samples. CONCLUsION： The expression of p53, RB1 and c-los genes appears to have a key role in the pathogenesis of hepatocellular carcinoma in Iran. simultaneous overexpression of these genes is significantly associated with their loss of expression during development of hepatocellular carcinoma.

关键词： Hepatocellular Carcinoma Iran Expressionof p53, cyclinD1, RB1, c-fos and N-ras genes

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susceptibility patterns and virulence genotypes of Helicobacter pylori affecting eradication therapy outcomes among Egyptian patients with gastroduodenal diseases

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World Journal of Gastroenterology 2023年第19期29卷 2950-2960页

作者： Ahmed Morad Asaad Gasser El-Azab Eman Abdelsameea Osama Elbahr Ahmed Kamal Mohamed Abdel-samiee Ahmed Abdelfattah Heba Abdallah Doha Maher Ahmed El-Refaie samar Ebrahim Ghanem shamshul Ansari samah Mohammed Awad Department of Microbiology College of MedicineZagazig UniversityZagazig 44519Egypt Department of Hepatology and Gastroenterology National Liver InstituteMenoufia UniversityShebin El-Kom 32511Egypt Department of Clinical Pathology National Liver InstituteMenoufia UniversityShebin El-Kom 32511Egypt Department of Pathology National Liver InstituteMenoufia UniversityShebin El-Kom 32511Egypt Department of Clinical Biochemistry and Molecular Diagnostics National Liver InstituteMenoufia UniversityShebin El-Kom 32511Egypt Department of Health sciences Higher Colleges of TechnologyAbu Dhabi Women's CollegeAbu Dhabi 25026United Arab Emirates Department of Clinical Microbiology and Immunology National Liver InstituteMenoufia UniversityShebin El-Kom 32511Egypt

BACKGROUND Helicobacter pylori(***)is a significant human pathogen that is responsible for a variety of illnesses,including mucosa-associated lymphoid tissue lymphoma,gastric cancer,peptic ulcers,and *** To investigate the frequency of *** infection and its resistance patterns among Egyptian patients and to determine the influence of *** virulence genetic determinants on the eradication success of 14-d triple therapy *** *** infections were investigated in 72 patients with gastroduodenal complications suggestive of *** *** cagA and vacA genotypes of cultured strains were studied using polymerase chain *** patients underwent 14 d of triple-therapy *** treatment response was examined using histology and a rapid urease test 6 wk after therapy *** The intention-to-treat eradication rate was 59.2%(95%CI:48.2%-70.3%).Rates of *** resistance to clarithromycin,amoxicillin,and metronidazole were 52.8%,81.9%,and 100%,*** eradication of *** was more significantly associated with vacA s1-positive strains[adjusted odds ratio(aOR)=0.507,95%CI:0.175-0.822].A significant association was found between failed eradication rate and *** strains resistant to clarithromycin(aOR=0.204,95%CI:-0.005 to 0.412)and amoxicillin(aOR=0.223,95%CI:0.026-0.537).CONCLUsION This study’s low *** eradication rate following 14-d triple therapy is concerning and *** pan-resistance to metronidazole followed by the high resistance to ciprofloxacin,amoxicillin,and clarithromycin in this research is challenging and of great concern.

关键词： Helicobacter pylori Eradication therapy Virulence Clarithromycin resistance cagA gene vacA gene

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