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CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses

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World Journal of Gastrointestinal Oncology 2013年第3期5卷 50-59页

作者： Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar delgado Carlos Jaramillo María Mercedes Bravo Cancer and Infectious Agents Research Group National Cancer Institute Molecular Diagnostics and Bioinformatics Laboratory Biological Sciences Department Los Andes University

AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODs: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGs gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of "hummingbird" cells (>15 μm) was determined. REsULTs: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P s 26.2%±14.8%, Ps 21.5% ± 19.5%, Ps 29.5% ± 27.1%, P spectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGs cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P serve differences in the extent of cell

关键词： Helicobacter pylori cagA 3’region CagA protein Interleukin 8 Cell elongation Glu-Pro-Ile-Tyr-Ala

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Free vibration and transient response of double curved beams connected by intermediate straight beams

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 37-62页

作者： R.A.JAFARI-TALOOKOLAEI H.GHANDVAR E.JUMAEV s.KHATIR T.CUONG-LE school of Mechanical Engineering Babol Noshirvani University of Technology Department of Mechanical Engineering Faculty of Engineering New Uzbekistan University University of Business and science Center for Engineering Application and Technology solutions Ho Chi Minh City Open University

This paper investigates the free vibration and transient response of interconnected structures including double curved beams and intermediate straight beams,which are all constructed from functionally graded porous(FGP) materials. The strain potential and kinetic energies of each beam along with the work done by the external force are calculated. Additionally, a higher-order beam element is introduced to derive stiffness and mass matrices, along with the force vector. The curved and straight beams are discretized, and their assembled stiffness, mass matrices, and force vectors, are *** conditions at the joints are used to derive the total matrices of the entire structure. subsequently, the natural frequencies and transient response of the system are determined. The accuracy of the mathematical model and the self-developed computer program is validated through the comparison of the obtained results with those of the existing literature and commercial software ANsYs, demonstrating excellent ***, a comprehensive study is conducted to investigate the effects of various parameters on the free vibration and transient response of the considered structure.

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sex steroids of black scabbardfish,Aphanopus carbo,in relation to reproductive and migratory dynamics

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Aquaculture and Fisheries 2021年第1期6卷 42-50页

作者： Inês Farias Elsa Couto Neide Lagarto Joāo delgado Adelino V.M.Canário Ivone Figueiredo Department of sea and Marine Resources Portuguese Institute for Sea and Atmosphere(IPMA)Avenida Doutor Alfredo Magalhāes Ramalho 6Algés1495-165Portugal Mediterranean Institute for Advanced studies(IMEDAE) Esporles07190Spain Centre of Marine sciences(CCMAR-CIMAR) University of AlgarveCampus de GambelasFaro8005-139Portugal Regional Fisheries Directorate Research Service(DSI-DRP)Estrada da Pontinha S/NFunchal9004-562Portugal Interdisciplinary Centre of Marine and Environmental Research(CIIMAR-CIMAR) Rua Dos BragasPorto4050-123Portugal

Black scabbardfish,Aphanopus carbo,is a commercially important species that takes distant migrations throughout its life *** steroids were measured by radioimmunoassay in the blood plasma of specimens caught off the Madeira Archipelago and mainland Portugal to link this species migratory path with its reproductive ***,a pilot study using Mozambique tilapia(Oreochromis mossambicus)was designed to evaluate the effect of sample freshness on steroid levels because black scabbardfish blood was collected at separate times after specimens were *** changes in T and 11-KT concentrations between the time of blood extraction and the time after preservation did not statistically differ among the different methods ***,measured black scabbardfish steroid concentrations were directly used in the subsequent data *** females,E2 and in T concentrations peaked at a late stage of vitellogenesis.E2 concentration was significantly different between females caught off each *** E2 and T concentrations from all developing females resulted in the separation of two distinct groups,independently of their geographical *** males,T and 11-KT were not significantly different between maturity *** hepatosomatic index of males caught off mainland Portugal was relatively *** may reflect a mechanism for storing energy that will later be consumed during migration to the spawning *** trend of sex steroids concentrations throughout the sexual maturation of the species is consistent with the morphological indicators and shows evidence of the reproductive and migratory pattern hypothesised for the black scabbardfish in NE Atlantic.

关键词： Black scabbardfish Deep-sea Madeira Mainland Portugal Migration sex steroids

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A local spatial decision support system for developing countries based on MCA,fuzzy sets and OWA–case study of a municipality in Cuba

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Geo-spatial Information science 2013年第2期16卷 120-129页

作者： Ricardo delgado TELLEZ ZHONG Ershun ZUHU Yang Arisleydis PEÑA DE LA CRUZ Graduate University of Chinese Academy of sciences No.A19Yuquan RoadBeijing 100049China state Key Laboratory of Resources and Environmental Information system Institute of Geographic Sciences and Natural Resources ResearchCASNo.A11Datun RoadBeijing 100101China International Business Division SuperMap Software Co.LtdNo.A10Jiuxianqiao North RoadBeijing 100015China Department of Applied Meteorology Cuban Institute of MeteorologyHavana 95100Cuba

This paper presents a spatial Decision support system for local governments of developing *** allows municipality government,enterprises,scientific community and civil society to address decision problems using *** framework is supported by four modules of information technologies:Environmental Decision support Database,Data Manipulation,Decision support,and Mapping.A case study is presented covering the implementation of this framework in one municipality of *** example of land suitability planning for coconut crops is used to evaluate the system performance and *** show local municipalities are able to use this framework to solve local decision problems using state of the art decision making even with low infrastructure development.

关键词： GIs spatial Decision support system MCA OWA developing countries

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Aedes-borne viral infections and risk of emergence/resurgence in sub-saharan African urban areas

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Journal of Biosafety and Biosecurity 2020年第2期2卷 58-63页

作者： Alexandre Zerbo Rafael Castro delgado Pedro Arcos Gonzalez Unit for Research in Emergency and Disaster Faculty of Medicine and Health SciencesUniversity of Oviedo33006 OviedoSpain

The sub-saharan African region is increasingly becoming urbanized with the creation of an urban ecology that is conducive to the emergence of vector-borne diseases,such as Aedes-borne viral *** viral infections pose a threat to urban health in sub-saharan *** major menacing arbovirus diseases in sub-saharan Africa include dengue,yellow fever,chikungunya,and Zika,since they have shown(re)emergence in the last *** this review,we analyzed the risk factors for the resurgence of these arboviral diseases as well as their epidemiology and burden by studying the *** results suggested that the risk of emergence of Aedes-borne viral infections was related to the urbanicity and competence of Aedes,the genetics of these viruses,environmental factors,and the socio-economic conditions of the sub-saharan population in urban *** public health awareness and appropriate epidemiological and vector surveillance are essential.

关键词： Dengue Yellow fever Chikungunya Zika Risk factors Urban areas sub-saharan Africa

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BAT-tling oxidative stress through BCAA catabolism

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Life Metabolism 2024年第5期3卷 42-44页

作者： Maria delgado-Martin Qiaoqiao Zhang Lawrence Kazak Rosalind&Morris Goodman Cancer Institute and Department of Biochemistry McGill UniversityMontrealQCH3A 1A3Canada Department of Biochemistry McGill UniversityMontrealQCH3G 1Y6Canada

Elevated circulating levels of branched-chain amino acids(BCAAs)are associated with the development of type 2 diabetes and obesity,diseases that can be countered by the energy dissipating(thermogenic)function of brown adipose tissue(BAT).In a recent study published in Cell,Verkerke and colleagues report that BAT promotes insulin sensitivity in the liver by coupling antioxidant homeostasis with BCAA catabolism,an effect that is independent of its thermogenic properties.

关键词： BCAA properties. homeostasis

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A review of the risk of cholera outbreaks and urbanization in sub-saharan Africa

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Journal of Biosafety and Biosecurity 2020年第2期2卷 71-76页

作者： Alexandre Zerbo Rafael Castro delgado Pedro Arcos Gonzalez Unit for Research in Emergency and Disaster Faculty of Medicine and Health SciencesUniversity of Oviedo33006 OviedoSpain

The sub-saharan region of Africa is rapidly urbanizing,that has consequently spurred the development of specific challenges in terms of access to basic services and ***,exposure to waterborne diseases,including cholera,has been a recurrent problem for the people residing in these urban *** is important to understand the different risks faced by people in urban areas as it might aid in preventing and controlling the spread of the disease.A review of literature was conducted,using specific selection criteria to obtain relevant publications that described the risk factors for cholera outbreak in urban areas of sub-saharan Africa.A perusal of available literature indicated that despite the heterogeneity in the urban areas of Africa,there are similarities with respect to the risk factors associated with the incidence of cholera *** risk factors include geographical location,hydroclimatic parameters,urban environment,genomics of the Vibrio cholerae virulence,sanitation,and human behavior.A succinct comprehension of the possible relationship between the different risk factors might prove to be helpful in managing the cholera epidemic.

关键词： Cholera Urban environment sub-saharan Africa

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Emerging role of A-kinase anchoring protein 5 signaling in reward circuit function

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Neural Regeneration Research 2025年第10期20卷 2913-2914页

作者： William J.Flerlage Mark L.Dell’Acqua Brian M.Cox Fereshteh s.Nugent Uniformed services University of the Health sciences Department of Pharmacology and Molecular TherapeuticsBethesdaMDUSA Department of Pharmacology University of Colorado School of MedicineAnschutz Medical CampusAuroraCOUSA

There is a strong evidence supporting the hypothesis of synaptic dysfunction as a major contributor to neural circuit and network disruption underlying emotional and mood dysregulation in psychiatric disorders(simmons et al.,2024).Diverse sets of distinct molecular signaling pathways converge on the synapse to regulate synaptogenesis,synaptic function,and synaptic plasticity in brain regions and circuits through complex interactions organized by numerous multivalent protein scaffolds,including the family of proteins known as A-kinase anchoring proteins(AKAPs).

关键词： function supporting

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Helicobacter pylori CagA protein polymorphisms and their lack of association with pathogenesis

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World Journal of Gastroenterology 2010年第31期16卷 3936-3943页

作者： Nicole Acosta Andrés Quiroga Pilar delgado María Mercedes Bravo Carlos Jaramillo Molecular Diagnostics and Bioinformatics Laboratory Biological Sciences DepartmentLos Andes University Infectious Agents and Cancer Research Group National Cancer Institute(Colombia)

AIM: To investigate Helicobacter pylori (H. pylori) CagA diversity and to evaluate the association between protein polymorphisms and the occurrence of gastric pathologies. METHODs: One hundred and twenty-two clinical isolates of H. pylori cultured from gastric biopsies obtained from Colombian patients with dyspepsia were included as study material. DNA extracted from isolates was used to determine cagA status, amplifying the C-terminal cagA gene region by polymerase chain reaction. One hundred and six strains with a single amplicon were sequenced and results were used to characterize the 3' variable region of the cagA gene. To establish the number and type of tyrosine phosphorylation motifs Glutamine acid-Proline-Isoleucine-Tyrosine-Alanine (EPI-YA) bioinformatic analysis using Amino Acid sequence Analyzer-Amino Acid sequence Analyzer software was conducted. Analysis of the association between the number of EPIYA motifs and the gastric pathology was performed using χ2 test and analysis of the presence of EPIYA-C motifs in relation to the pathology was made by logistic regression odds ratios. Comparisons among EPIYA types found and those reported in GenBank were performed using a proportion test in statistix Analytical software version 8.0. REsULTs: After amplification of the 3' of the cagA gene, 106 from 122 isolates presented a single amplicon and 16 showed multiple amplicons. As expected, diversity in the size of the cagA unique fragments among isolates was observed. The 106 strains that presented a single amplicon after 3' cagA amplification came from patients with gastritis (19 patients), atrophic gastritis (21), intestinal metaplasia (26), duodenal ulcer (22) and gastric cancer. DNA sequence analysis showed that the differences in size of 3' cagA unique fragments was attributable to the number of EPIYA motifs: 1.9% had two EPIYA motifs, 62.3% had three, 33.0% had four and 2.8% had five motifs. The majority of tested clinical strains (62.3%) were found to harbor

关键词： Helicobacter pylori CagA-protein polymorphisms Molecular characterization Bioinformatic analysis Pathogenesis Cancer

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Feather content of porphyrins in Eurasian eagle owl(Bubo bubo)fledglings depends on body condition and breeding site quality

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Integrative Zoology 2018年第5期13卷 569-578页

作者： Ismael GALVÁN María del Mar delgado Pablo RCAMARERO Rafael MATEO RuiLOURENÇO Vincenzo PENTERIANI Department of Evolutionary Ecology Doñana Biological Station-CSICSevillaSpain Research Unit of Biodiversity-UOCsIC-PA Oviedo UniversityMieresSpain Institute for Game and Wildlife Research IREC-CSIC-UCLM-JCCMCiudad RealSpain Labor-Laboratório de Ornitologia Instituto de Ciências Agrárias e Ambientais MediterrânicasUniversity of EvoraÉvoraPortugal Pyrenean Institute of Ecology(IPE)-CsIC ZaragozaSpain

Porphyrins are pigments produced in most animal cells during the synthesis of heme,but their importance for external coloration is ***(Order strigiformes)are among the few animals that accumulate porphyrins in the integument,where it could serve as a means of *** we hypothesized that the porphyrin content of feathers may depend on body condition and breeding site quality in Eurasian eagle owl(Bubo bubo)fledglings and,thus,constitute amplifiers of the quality of the area where they are *** high-performance liquid chromatography,we found 2 porphyrins(protoporphyrin IX and coproporphyrin III)in the body feathers of 19 eagle owl fledglings from 7 breeding *** III,but not protoporphyrin IX feather concentration,was positively associated with the body mass of fledglings and with the quality of the breeding sites where they were reared with respect to food quality and *** coproporphyrin III is produced under oxidative stress,we suggest that good breeding sites may lead to fledglings in good ***,in turn,may make fledglings induce a certain level of free radical and coproporphyrin III production to signal to conspecifics their site-mediated capacity to cope with oxidative *** is the first time that porphyrin content in the integument has been found to be related to individual quality,opening a new scenario for studying evolution of animal coloration.

关键词： amplifiers condition-dependence eagle owl fluorescence porphyrins

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