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Advances in Biological Chemistry 2024年第1期14卷 1-15页

作者： salomon Madjitoloum Betoloum séverin mbaihougadobe Abel Mbaiogaou Djibrine Adoum Oumar Mbaindiguim Dagoto Yaya Mahmout Laboratory of Research on Natural substances Faculty of Exact and Applied Sciences (F.E.A.S) University of N’Djamena N’Djamena Chad Department of Chemistry University of Moundou Moundou Chad

The aim of the present work is to assess the value of Detarium senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity of Detarium senegalense extracts. For this purpose, sequential extraction using solvents of increasing polarity was essential. The various extracts obtained underwent phytochemical and biochemical analyses. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthocyanins and steroids/terpenes. Quantitative analysis of total polyphenols, total flavonoids and total anthocyanins yielded the following results: total flavonoids (0.803 ± 0029 mg EQ/100g P for acetone extract of roots and 0.871 ± 0.401 mg EQ/100g P for methanol extract of leaves);total polyphenols (23.298 ± 12.68 mg EAG/100g P for acetone extract of roots and 24.69 ± 0.49 401 mg EAG/100g P for methanol extract of leaves);total monomeric anthocyanins (44.697 ± 0.939 mg EC3G/100g P and 16.699 ± 0.193 mg EC3G/100g P respectively for acetone and methanol extracts of stem bark). DPPH free radical scavenging activity was 1.674 ± 0.023 mg/mL for the acetone extract and 0.934 ± 0.24 mg/mL for the methanol extract of roots. .

关键词： Total Phenols Total Flavonoids Total Anthocyanins Antioxidant and Anti-Radical Activities Detarium senegalense Chad

Phytochemical Tests, Assessment of Antioxidant Properties and Isolation of Two Compounds of Ethyl Acetate Extract of Chadian Propolis: Case of Bebotho (southern Chad)

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American Journal of Analytical Chemistry 2022年第7期13卷 241-254页

作者： salomon Madjitoloum Betoloum Abel Mbaiogaoun severin mbaihougadobe Emmanuel Talla Department of Chemistry Faculty of Exact and Applied Sciences (FSEA) University of N’Djamena N’Djamena The Republic of Chad Department of Chemistry Faculty of Science University of Ngaoundéré (UN) Ngaoundéré The Republic of Cameroon

The present work deals with the research of chemical constituents and evaluation of antioxidant properties of Bebotho propolis. From the ethyl acetate extract, we isolated, using various chromatographic techniques, a mixture of two identical compounds (isomers) indexed PBy4a and PByb. The structures of these compounds were elucidated by means of spectroscopic analysis techniques (Ms, IR, sup>1sup>H-NMR, sup>13sup>C-NMR, HMBC and HsQC) and by comparison of the spectral data with those described in the literature. Thus, these compounds were identified to a mixture of two chromones namely 5,7-dihydroxy-2-methylchromone-6-C-α-D-glucopyranoside and 5,7-dihydroxy-2-methylchromone-8-C-β-D-glucopyranoside, first reported in propolis. The study of the antiradical power, chelating power and the quantification of phenolic compound of these same extracts, showed interesting properties that propolis extracts have to scavenge free radicals.

关键词： Isolation Propolis Chelating Power 5 7-Dihydroxy-2-Methylchromone-6-C-α-D-Glucopyranoside and 5 7-Dihydroxy-2-Methylchromone-8-C-β-D-Glucopyranoside

Isolation and Characterization of Anthocyanins in Four Varieties of Vigna subterranea (Fabaceae)

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International Journal of Organic Chemistry 2022年第2期12卷 102-115页

作者： Abel Mbaiogaou salomon Madjitoloum Betoloum severin mbaihougadobe Michel Naitormbaide Adama Hema Eloi Pale Training and Research Unit in Exact and Applied sciences Laboratory of Organic Chemistry and Applied Physic Department of Chemistry University de Ouagadougou Ouagadougou Burkina Faso Laboratory of Research on Natural substances Faculty of Pure and Applied Sciences (F.P.A.S.) University of N’Djamena N’Djamena Chad Chadian Institute of Agronomic Research for Development (CIARD) Deli Chad

By chromatographic methods (HPLC, TLC) coupled with spectral methods (LC-Ms, TLC-Ms) and chemical revelation tests, anthocyanins from four Vigna subterranea varieties (M4, D3, KVs350, KVs97) were isolated and identified as malvidin 3-O-β-D-glucopyranoside, paeonidin-3-O-β-D-glucopyranoside, petunidin-3-O-β-D-glucopyranoside, cyanidin 3-O-β-D-glucopyranoside, del- pinidin-3-O-β-D-glucopyranoside.

关键词： Fabaceae Vigna subterranea Malvidin Paeonidin Petunidin Cyanidin Delphinidin

Quantification of Tannins in Four species of Genus Mucna seeds

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Advances in Biological Chemistry 2022年第4期12卷 73-80页

作者： Abel Mbaiogaou severin mbaihougadobe salomon Madjitoloum Betoloum Adama Hema Eloi Pale samaté Dorosso Abdoul Laboratory of Research on Natural substances Faculty of Pure and Applied Sciences (F.P.A.S.) University of N’Djamena N’Djamena Chad Laboratory of Organic Chemistry and Applied Physics Department of Chemistry UFR-SEA University of Ouagadougou Ouagadougou Burkina Faso

In order to make Mucuna seeds consumable, seeds are treated according to the following procedures: boiling, soaking followed by boiling in the presence of 0.5% KOH and finally soaking for a long time followed by boiling in the presence of 0.5% KOH, so that tannins can be removed. Preliminary studies done on the raw seeds of four Mucuna species revealed the presence of tannins. The results showed that the crushed seeds contained all high tannin with the content of 0.370 g GAE/100g DM, 0.336 g GAE/100g DM;0.266 g GAE/100g DM and 0.203 g GAE/100g DM for Mucuna deerigiana, Mucuna nagaland, Mucuna cochinchinensis and Mucuna rajada respectively. The different methods of seed treatment significantly reduced the tannin content in Mucuna seeds. The method of soaking the seeds for 12 hours and then scalding in the presence of 0.5% KOH reduced tannins in Mucuna deerigiana, Mucuna nagaland, Mucuna cochinchinensis, and Mucuna rajada seeds by 47.29%;48.21%;66.16%;and 15.76% respectively. This method gave the highest tannin reduction rate.

关键词： Quantification Tannins Reduction Rate Mucuna Burkina-Faso