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Chemical Composition and Antiviral Effect of Extracts of Origanum vulgare

Advances in Bioscience and Biotechnology 2019年第7期10卷 188-196页

作者： Daiane Einhardt Blank silvia de Oliveira Hübner Gabriela Hornke Alves Claudia Andrea lima Cardoso Rogério Antonio Freitag Marlete Brum Cleff departamento de Química Universidade Federal de VicosaVicosaMGBrazil departamento da Veterinária Universidade Federal de PelotasPelotasRSBrazil Center of Chemical Pharmaceutical and Food SciencesUniversidade Federal de PelotasPelotasRSBrazil departamento de Química Universidade Estadual de Mato Grosso do Sul(UEMS)DouradosMSBrazil

This study aimed determine the activity of aqueous and ethanolic extracts of Origanum vulgare against some viruses of veterinary importance (bovine viral diarrhea virus (BVDV), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV), canine distemper virus (CDV), canine adenovirus (CAV), and canine cororavirus (CCoV) by evaluating the possibility of inhibition of viral particles production. The aqueous extract from 1600 μg/ml did not show cytotoxicity for all cellular lineages evaluated, Madin Darby bovine kidney cells (MDBK), Rabbit kidney cells (RK 13), Madin Darby canine kidney cells (MDCK) and Crandell feline kidney cells (CRFK), and the ethanolic extract of Origanum vulgare was not toxic at 600 μg/ml. The addition of aqueous extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 infecting dose for cellular culture at 50% (TCID50) to 102.09 TCID50/100 μl while in the presence of the ethanolic extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 TCID50 to 100.79 TCID50/100 μl. To CDV the addition of aqueous extract resulted in a reduction from 102.00 TCID50 to 100.00 TCID50/100 μl while in the presence of the ethanolic extract titers were reduced from 102.00 TCID50 to 101.50 TCID50/100 μl. No significant differences in titers regarding the others analyzed viruses were detected. With respect to chemical analysis of the extracts of Origanum vulgare, were identified in the ethanol extract phenolics rosmarinic acid, caffeic acid, carnosol, p-coumaric acid, carnosic acid, luteolin, apigenin, kaempferol and quercetin. In aqueous extracts of Origanum vulgare were detected rosmarinic acid, p-coumaric acid carnosic acid, luteolin, apigenin, kaempferol and quercetin. The data obtained stimulate other biological assays in order to determine which compounds are responsible for the antiviral activity as well as which are the mechanisms involved. The res

关键词： Virus Origanum vulgare Phenolics Cytotoxicity

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Bladder Neck Morphologic Changes and Clinical Correlation of smokers submitted to Radical Prostatectomy

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Open Journal of Urology 2013年第6期3卷 227-231页

作者： l. C. N. Araujo W. s. Costa F. O. Vilar s. V. C. lima F. J. B. sampaio Urogenital Research Unit State University of Rio de Janeiro RJ Brazil Urology department Hospital das Clinicas Federal University of Pernambuco PE Brazil

Introduction: smoking is an important risky factor to many diseases, affecting arterial system, skin and urogenital system, including bladder neck stenosis. Its effect on bladder neck has not been described. Objective: Evaluate possible morphological changes caused by nicotine in the bladder neck. Material and Method: Fragments of bladder neck of 16 patients were submitted to stereological analysis, and those patients are divided into two groups, one of smokers and the other of non-smokers with 7 and 9 patients, respectively. After 90 days of surgery, they were submitted to free uroflowmetry and data analyzed by T test, having statistical significance with P 0.05. Results: An increase of 63.26% in the amount of fibers in the elastic system of the smokers group was observed, a reduction of 35.96% in the thickness of arteries, as well as an increase of IPss and decrease of maximum flow in uroflowmetry, all with statistical significance. Discussion: laboratorial changes are similar to those found in other studies with different tissues, such as skin, in which those findings are related to premature ageing. Clinical results, though statistically significant, do not have clinical consistence because the study was meant to morphological analysis. Conclusion: smoking increases the amount of fibers in the elastic system and decreases the thickness of bladder neck arteries.

关键词： smoking Bladder Neck Elastic Fibers Uroflowmetry

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Characterization of the Biological Potential of the Essential Oils from Five species of Medicinal Plants

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American Journal of Plant sciences 2017年第2期8卷 154-170页

作者： Danubia Aparecida Carvalho selvati Rezende Rafaela Vieira souza Maisa lamounier Magalh?es Alex Rodrigues silva Caetano Marcos schleiden sousa Carvalho Ellen Cristina de souza luiz Gustavo de lima Guimaraes David lee Nelson luís Roberto Batista Maria das Gra?as Cardoso departamento de Química Universidade Federal de LavrasLavrasBrasil departamento de Ciencia dos Alimentos Universidade Federal de LavrasLavrasMGBrasil departamento de Ciência dos Alimentos Universidade Federal de LavrasLavrasMGBrasil departamento de Agricultura Universidade Federal de LavrasLavrasMGBrasil Universidade Federal de sao Joao del-Rei Sao Joao del-ReiBrasil Universidade Federal do Vale do Jequitinhonha e Mucuri DiamantinaMGBrasil

Essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation, chemically characterized and quantified by GC/Ms and GC/FID. The principal componentes in the essential oil of M. piperita (carvone and limonene), C. citratus (geranial, neral and myrcene), R. officinalis (1,8-cineole, camphor and α-pinene), P. boldus (α-terpinyl formate, p-cymene and 1,8-cineole) and F. vulgare (methyl chavicol, limonene and fenchone) were identified. The oils were tested for antioxidant activity employing the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical-capture method, the oxidation of the β-carotene/linoleic acid system, and the ABTs radical-capture method. The essential oils were not effective. The antioxidant activities of the oils were determined by the hydroxyl method, and the highest activity (62.80%) was observed with the essential oil from M. piperita. Activities of 1.54 and 1.82 μg μg-1, respectively, were observed for the essential oils from C. citratus and P. Boldus using the phosphomolybdenum method. No activity was observed with respect to reducing power. The essential oil from C. citratus was effective in inhibiting the growth of l. monocytogenes (15.63 mg·l-1), s. choleraesuis (15.63 mg·l-1) and E. coli (31.25 mg·l-1), and the essential oil from P. boldus inhibited the growth of s. aureus (62.5 mg·l-1). No essential oil inhibited the growth of P. aeruginosa. A low antioxidant activity and a promising antibacterial activity were observed for these essential oils.

关键词： Natural Products Antioxidant Antibacterial Activity

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Use of silicon in Mitigating Ammonium Toxicity in Maize Plants

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American Journal of Plant sciences 2015年第11期6卷 1780-1784页

作者： Cid Naudi silva Campos Renato de Mello Prado Cassiano Garcia Roque Antonio Joao de lima Neto luiz Junior Pereira Marques Aridênia Peixoto Chaves Claudia Amaral Cruz department of soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil department of soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Universidade Federal de Mato Grosso do sul Chapad&atildeo do Sul Brazil Universidade Federal de Vi&ccedilosa Vi&ccedilosa Brazil department of Applied Biology and Agriculture Universidade Estadual Paulista/FCAV Jaboticabal Brazil department of Plant science Universidade Federal Rural do Semi-árido Mossoró Brazil M.sc. in Agronomy Natal Brazil

silicon is a beneficial element that can mitigate abiotic stresses, such as ammonium toxicity. The objective herein was to evaluate the effects of silicon (si) on mitigating toxicity caused by excess ammonium in maize plants grown in nutrient solution. An experiment was conducted with maize plants (cultivar DKB 390 VT Pro II) grown in a greenhouse in pots (8 l) in a hydroponic system. The treatments were arranged in a 2 × 2 factorial, consisting of two ammonium concentrations (30 and 60 mmol·l-1) in the absence and presence of si (10 mmol·l-1), arranged in a completely randomized design with six repetitions. At 28 days after applying the treatments the dry mass of shoots and roots was evaluated, along with accumulation of silicon and nitrogen in the shoots. The use of silicon resulted in increases in the studied variables, regardless of ammonium concentrations. silicon reduced the effect of toxicity caused by ammonium excess in maize plants, resulting in greater growth and dry matter accumulation.

关键词： Zea mays l. Beneficial Element Nitrogen Nutritional Disorder Abiotic stress

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Influence of Cold storage on the Bioactivity Properties and the Quality of the Juice of Moro Blood Orange(Citrus sinensis(l.)Osbeck)

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American Journal of Plant sciences 2019年第1期10卷 24-37页

作者： Maisa lamounier Magalhaes luiz Carlos de Oliveira lima Allan da silva lunguinho Danubia Aparecida de Carvalho selvati Rezende Vanuzia Rodrigues Fernandes Ferreira Rafaela Magalhaes Brandao Josefina Aparecida de souza Ellen Cristina de souza Kátia Júlia de Almeida David lee Nelson Maria Das Gracas Cardoso departamento de Ciencia dos Alimentos Universidade Federal de Lavras(UFLA)LavrasBrazil departamento de Química Universidade Federal de Lavras(UFLA)LavrasBrazil Programa de Pos-Graduacao em Biocombustívis Universidade Federal do Vale do Jequitinhonha e MucuriDiamantinaBrazil

The possibility of commercialization of Moro blood oranges in tropical countries such as Brazil was evaluated to verify whether post-harvest management through storage at low temperatures for a period of 60 days can improve the bioactive properties and quality parameters. Moro blood oranges cultivated in Brazil did not contain significant amounts of anthocyanins at the time of harvesting, but these compounds were activated by post-harvest management through storage at low temperatures (4°C and 8°C) for a period of 60 days. The emergence of the anthocyanins in the juices occurred within a few weeks of storage, but the maximum levels were attained after 60 days and at the temperature of 8°C. Cold storage positively influenced other bioactive compounds such as total phenolic compounds, individual phenolic compounds, β-carotene and the antioxidant activity determined by the sequestration of DPPH free radicals. It did not influence the vitamin C content. In addition, storage significantly altered the color, total acidity and pH of the fruits, but it did not prevent its commercial use. The remaining quality parameters were not influenced. It is possible to commercialize these oranges in Brazil through post-harvest management.

关键词： Cyanidin-3-Glycoside Antioxidant Post-Harvest

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sensitivity of a Rapid Mix Test with Combined synthetic Antigens derived from Mycobacterium leprae PGl-1 for Diagnosis and surveillance of leprosy

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Open Journal of Immunology 2020年第1期10卷 1-9页

作者： Maria do Perpétuo socorro Amador silvestre Maxwell Furtado de lima Antonio G.P.Pereira Aline Rodrigues Bernardo Rafaela lopes Diniz Pedro Paulo Ferreira Ribeiro Edimílson Domingos da silva luana Nepomuceno Gondim Costa lima Instituto Evandro Chagas Rodovia BR-316km 07AnanindeuaParáBrasil Programa de desenvolvimento Tecnologico de reativos para diagnostico GPRED-Bio Manguinhos-FioCruzRio de JaneiroBrasil

Introduction: In the Americas, Brazil contributes 91.63% of the total cases and the state of Pará still has high endemia for leprosy. Objective: To analyze the performance of a rapid test for the diagnosis and epidemiological surveillance of leprosy in endemic areas. Methods: The sample consisted of 70 MB multibacillary leprosy (MB) patients, 63 paucibacillary (PB) patients, and 80 intradomiciliary consanguineous contacts (ICsCO) of patients. A rapid test with a 15-minute reading was applied using two prototypes: prototype 1, double test with trisaccharide antigen (NT-P-BsA) at 1a. line (83.2 ng/test) and disaccharide antigen (ND-O-BsA) at 2a. (83.2 ng/test), both with a flow of 0.08 μl/mm with a 10 μC membrane, anti-IgM conjugate with a flow of 0.040 μl/mm and a Tris-Triton and prototype 2 runner buffer with MIX antigen (trisaccharide + disaccharide) in the same concentrations and conditions of prototype 1. Results: The comparison of the MIX test positivity rate and the disaccharide or trisaccharide doublet test across all samples was statistically significant, demonstrating that the MIX test had higher seropositivity rates compared to the ND-O-BsA or NT-P-BsA. It was demonstrated that the MIX test showed a good performance, with 25.39% of the PB patients negative for the disaccharide and trisaccharide duplet test, but positive for MIX. Conclusions: These data suggest the potential for further optimizing the performance by adding other synthetic antigens to the MIX antigens.

关键词： leprosy Diagnosis Rapid Test

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Effect of Adjunct Culture <i>lactobacillus helveticus</i>(B02) on the Composition, Proteolysis, Free Amino Acids Release and sensory Characteristics of Prato Cheese

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Food and Nutrition sciences 2017年第5期8卷 512-525页

作者： Natália Chinellato de Azambuja Izildinha Moreno Darlila Aparecida Gallina leila Maria spadoti Eliana Maria Pettirossi Motta Maria Teresa Bertolodo Pacheco Angela lima Menêses de Queiroz Adriane Elisabete Costa Antunes Faculdade de Ciências Aplicadas Universidade Estadual de Campinas (FCA/UNICAMP) Limeira Brazil Centro de Tecnologia de laticínios Instituto de Tecnologia de Alimentos (TECNOLAT/ITAL) Campinas Brazil Faculdade de Engenharia de Alimentos Universidade Estadual de Campinas (FEA/UNICAMP) Campinas Brazil Centro de Ciência e Qualidade de Alimentos Instituto de Tecnologia de Alimentos (CCQA/ITAL) Campinas Brazil Centro de Tecnologia Universidade Federal da Paraíba Jo&#227o Pessoa Brazil

At semi-industrial scale it was obtained two cheeses, one with aromatic culture (CHN) and the other with also l. helveticus (CHN + lH). Proteolysis during ripening was evaluated using proteolytic indices, electrophoretic profile Urea-PAGE and release of free amino acids. sensory evaluation was performed by acceptability test. It was observed a 5-fold increase in the fraction corresponding to αs1-I-casein in the CHN cheese and 6.5 times in the CHN + lH cheese and 7.2 and 8.2 increase respectively, for total amino acids. Prato cheese with the adjunct culture showed higher acceptability in terms of overall aroma and flavor.

关键词： Amino Acids Cheese l. helveticus Ripening sensory Analysis

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Reserpine-Induced Progressive Parkinsonism in Mice Predisposed and Non-Predisposed to depressive-like Behavior

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Journal of Behavioral and Brain science 2021年第11期11卷 267-279页

作者： Manuela B. l. soares leonardo B. lopes-silva Marcela Becegato Vinicius s. Bioni Alvaro C. lima Gabriela M. Ferreira Ywlliane Meurer Regina H. silva Behavioral Neuroscience laboratory Department of Pharmacology Federal University of S&atilde o Paulo S&atilde o Paulo Brazil Memory and Cognition studies laboratory Post-Graduate Program of Cognitive Neuroscience and Behavior Department of Psychology Federal University of Paraíba Jo&atilde o Pessoa Brazil

Parkinson’s disease (PD) is a progressive degenerative condition that mainly affects the elderly. The disease comprises motor symptoms such as tremors at rest, loss of voluntary movement, decreased muscle strength, propensity to lean forward and acceleration of the walking pace. These signs are related to the degeneration of the nigrostriatal dopaminergic pathway. Patients also have non-motor symptoms, among which sleep alterations, cognitive deficits, fatigue, pain and depression stand out. Although depression has been described as the most prevalent non-motor symptom, it is not clear whether this mood disorder is due to PD or patients would already have a greater predisposition. The present study evaluated the relationship between the predisposition to depressive-like behavior and the development of motor alterations in a progressive pharmacological model of PD in mice. Mice were classified into groups of depressive-like propensities and submitted to the pharmacological model. Reserpine was administrated at 0.1 mg/kg on alternate days for 40 days. The catalepsy and oral movement tests were used to evaluate motor alterations, the sucrose preference test was used to evaluate anhedonia, and the open field test was applied to evaluate general activity. Reserpine promoted parkinsonian motor impairments, and there were no differences between animals from different depressive-like behavior profiles. Thus, it was not possible to find a relationship between parkinsonism and the propensity to depression based on the basal sucrose preference test. More studies with other evaluations of depressive-like behavior are needed to confirm the results found in our study.

关键词： Parkinson’s Disease depression Reserpine Mice

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Relationship between Th17 immune response and cancer

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World Journal of Clinical Oncology 2021年第10期12卷 845-867页

作者： Hanna santos Marques Breno Bittencourt de Brito Filipe Antônio França da silva Maria luísa Cordeiro santos Júlio César Braga de souza Thiago Macêdo lopes Correia luana Weber lopes Nayara silva de Macêdo Neres Rafael santos Dantas Miranda Dórea Anna Carolina saúde Dantas lorena lôbo Brito Morbeck Iasmin souza lima Amanda Alves de Almeida Maiara Raulina de Jesus Dias Fabrício Freire de Melo Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45083-900BahiaBrazil Instituto Multidisciplinar em saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Cancer is the second leading cause of death worldwide and epidemiological projections predict growing cancer mortality rates in the next *** has a close relationship with the immune system and,although Th17 cells are known to play roles in the immune response against microorganisms and in autoimmunity,studies have emphasized their roles in cancer *** Th17 immune response profile is involved in several types of cancer including urogenital,respiratory,gastrointestinal,and skin *** type of immune response exerts pro and antitumor functions through several mechanisms,depending on the context of each tumor,including the protumor angiogenesis and exhaustion of T cells and the antitumor recruitment of T cells and neutrophils to the tumor *** other factors,the paradoxical behavior of Th17 cells in this setting has been attributed to its plasticity potential,which makes possible their conversion into other types of T cells such as Th17/Treg and Th17/Th1 ***(Il)-17 stands out among Th17-related cytokines since it modulates pathways and interacts with other cell profiles in the tumor microenvironment,which allow Th17 cells to prevail in ***,the Il-17 is able to mediate pro and antitumor processes that influence the development and progression of various cancers,being associated with variable clinical *** understanding of the relationship between the Th17 immune response and cancer as well as the singularities of carcinogenic processes in each type of tumor is crucial for the identification of new therapeutic targets.

关键词： Cancer Immune response Th17 cells Interleukin-17 Tumor microenvironment Pathophysiology

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由电阻率测井曲线换算虚速度测井曲线的一种新方法

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石油物探译丛 1989年第1期 22-28页

作者： W. l. Brito Dos santos T. J. Ulrych O. A. l. de lima 高騊

本文介绍了一种利用电阻率值生成虚速度测井曲线的方法。研究出了应用于各种岩性的电阻率与传播时间之间的理论关系。它是利用了一种将岩石电阻率带定义为与实际地层有关的岩性层並能显示电阻率微小变化的方法进行的,这种关系的应用结... 详细信息

本文介绍了一种利用电阻率值生成虚速度测井曲线的方法。研究出了应用于各种岩性的电阻率与传播时间之间的理论关系。它是利用了一种将岩石电阻率带定义为与实际地层有关的岩性层並能显示电阻率微小变化的方法进行的,这种关系的应用结果已在巴西Bahia区的Reconcavo沉积盆地中得到了验证。由五口井换算的虚速度测井曲线与实际声测井曲线对比结果表明了本方法的有效性。

关键词：电阻率测井虚速度测井曲线换算

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