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Journal of Applied Mathematics and Physics 2021年第5期9卷 1171-1179页

作者： Satoshi Iwakami Masahiko Tamega Masahide Sanada Michiaki Mohri Yoshitaka Iwakami Naoki Okamoto ryousuke asou Shuji Jimbo Masaji Watanabe Earth Rise Company Inc. Okayama Japan Visiting Researcher Okayama University Okayama Japan Specially Appointed Professor Okayama University Okayama Japan

This study focuses on change of topography in a water area. Output data from a GPS unit and an echo sounder data were incorporated into analysis for construction of underwater topography. Comparison of two data sets ... 详细信息

关键词： Underwater Topography RTK-GPS Echo Sounder

Mathematical Modeling and Computational Analysis of Underwater Topography with Global Positioning and Echo Sounder Data

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Mathematical Modeling and Computational Analysis of Underwat...

Low-velocity pneumatic conveying in horizontal pipe for coarse particles and fine powders

作者： Satoshi Iwakami Masahiko Tamega Masahide Sanada Michiaki Mohri Yoshitaka Iwakami Naoki Okamoto ryousuke asou Shuji Jimbo Masaji Watanabe Earth Rise Company Inc. Visiting Researcher Okayama University Specially Appointed Professor Okayama University

关键词： Underwater Topography RTK-GPS Echo Sounder

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Particuology 2008年第5期6卷 316-321页

作者： Yuji Tomita Vijay Kumar Agarwal Hiroyuki asou Katsuya Funatsu Kyushu Institute of Technology 1-1 SensuichoTobataKitakyushu 804-5500Japan Indian Institute of Technology Hauz KhasNew Delhi 110016India

First, the characteristics of low-velocity conveying of particles having different hardness are experimentally investigated in a horizontal pipeline in terms of flow pattern and pressure drop to show that the slug flow can be classified into two types depending on the settling of particles along the pipeline, and the period is small for slug flow without the settled layer, which is called solitary slug flow. The pressure drop for soft particles is shown to be larger than that for hard particles. Then, experimental results are presented on horizontal fluidized-bed conveying of fine powders to show that air release from the top surface of the conveying channel is an imnortant factor for high mass flow rate of particles.

关键词： Low-velocity conveying Slug flow Soft particle Horizontal fluidized-bed conveying Fine powder

Low-intensity pulsed ultrasound prompts tissue-engineered bone formation after implantation surgery

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Chinese Medical Journal 2014年第4期127卷 669-674页

作者： Wang Juyong Wang Juqiang asou Yoshinori Paul Fu Shen Huiliang Chen Jiani Sotome Shinichi Department of Orthopaedic Surgery Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Orthopaedics First Hospital of China Medical University Shenyang Liaoning 110001 China Department of Orthopaedic Surgery Graduate School Tokyo Medical and Dental University Tokyo 1138519 Japan Department of Neurological Surgery Wayne State University School of Medicine Detroit MI 48201 USA Department of Orthopaedic Surgery Graduate School Tokyo Medical and Dental University Tokyo 1138519 Japan COE Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone Tokyo Medical and Dental University Bunkyo-ku Tokyo 1138519 Japan

Background A practical problem impeding clinical translation is the limited bone formation seen in artificial bone ***-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds,enhanced production of bone marrow cells,and improved tissue-engineered bone *** goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells （BMSCs）.Methods BMSCs/β-tricalcium phosphate （β-TCP） composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound （LIPUS） treatment group and a control *** implants were harvested at 5,10,25,and 50 days after *** samples were then biomechanically tested and analyzed for alkaline phosphate （ALP） activity and osteocalcin （OCN） content and were also observed by light *** The levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control *** analysis revealed a greater degree of soft tissue repair,increased blood flow,better angiogenesis,and more extensive bone formation in the LIPUS groups than in the *** significant difference in the compressive strength was found between the two *** LIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β3-TCP composites.

关键词： β-tricalcium phosphate low-intensity pulsed ultrasound marrow stromal cells bone formation

成人T细胞白血病N－ras基因点突变的研究

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中华血液学杂志 1994年第7期15卷 356-358页

作者：王建祥郝玉书麻生范雄铃岛仁王立高月清天津中国医学科学院协和医科大学血液学研究所熊本大学第二内科

成人Ｔ细胞白血病（ＡＴＬ）由人类Ⅰ型嗜Ｔ细胞病毒（ＨＴＬＶ－Ⅰ）引起，但其发病机理仍不清楚。我们应用逆转录－多聚酶链反应结合直接序列分析检测１０例ＡＴＬ患者的Ｎ－ｒａｓ基因，结果在一例ＡＴＬ发现Ｎ－ｒａｓ６１位点突变... 详细信息

成人Ｔ细胞白血病（ＡＴＬ）由人类Ⅰ型嗜Ｔ细胞病毒（ＨＴＬＶ－Ⅰ）引起，但其发病机理仍不清楚。我们应用逆转录－多聚酶链反应结合直接序列分析检测１０例ＡＴＬ患者的Ｎ－ｒａｓ基因，结果在一例ＡＴＬ发现Ｎ－ｒａｓ６１位点突变（ＣＡＡ→ＣＴＡ），说明ＨＴＬＶ－Ⅰ感染之后有多种因素参与ＡＴＬ的发病。

关键词：白血病聚合酶链反应基因突变

低压细胞灌注多孔人工骨的成骨能力：与常压灌注的比较

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中国组织工程研究与临床康复 2008年第49期12卷 9601-9605页

作者：王居勇沈惠良麻生义则张庆明刘利民四宫谦一首都医科大学宣武医院骨科北京市100053 东京医科齿科大学医学部骨科日本

背景:尽管利用低压脱泡细胞灌注法在多孔生物材料携带成骨细胞的体外研究取得令人满意的进展,然而是否能够提高动物体内工程组织骨的成骨能力还需要进一步探讨。目的:采用低压方法在β-磷酸三钙材料内种植骨髓间充质干细胞,观察其复合... 详细信息

背景:尽管利用低压脱泡细胞灌注法在多孔生物材料携带成骨细胞的体外研究取得令人满意的进展,然而是否能够提高动物体内工程组织骨的成骨能力还需要进一步探讨。目的:采用低压方法在β-磷酸三钙材料内种植骨髓间充质干细胞,观察其复合体移植到大鼠体内后的成骨能力并与常压灌注法比较。设计、时间及地点:组织学观察和生化学分析,对比实验。于 2005-01/2006-02 在东京医科齿科大学医学部骨科实验室完成。材料:5 mm×5 mm×5 mm 多孔生物材料磷酸三钙;实验动物为 7 周雄性 Fischer-344 大鼠 8 只,4 只大鼠用于生化分析,另 4 只大鼠用于组织学分析。方法:在体外培养扩增骨髓间充质细胞后,将细胞置于成骨分化的培养液中,通过常压(101.1 kPa)和低压(13.3 kPa)2 种方法将细胞灌注入多孔生物材料磷酸三钙人工骨中。同时将 Fischer-344 背部两侧各取 2 个切口,低压种植的骨髓间充质干细胞/β-磷酸三钙移植到右侧皮下,常压种植的骨髓间充质干细胞/β-磷酸三钙移植到左侧皮下。主要观察指标:通过扫描电镜观察组织工程骨内细胞种植情况;组织学观察和计算机图像分析移植 3 周和 6 周后体内组织工程骨成骨情况;检测组织工程骨碱性磷酸酶活性。结果:扫描电镜观察低压细胞种植法灌注的多孔人工骨内存在更多的细胞;在移植后的第 3,6 周,低压组中碱性磷酸酶活性高于常压组,经过组织学观察和计算机图像分析处理,低压组比常压组有更均匀和更广泛的成骨量。结论:低压细胞灌注提高骨生成能力明显优于常压灌注方法。

关键词：磷酸三钙低压骨髓间充质干细胞骨形成

低压细胞灌注法提高多孔人工骨成骨能力的体外研究

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中国组织工程研究与临床康复 2007年第31期11卷 6130-6134页

作者：王居勇张怀华沈慧良麻生义则陈佳妮张庆明李旗王居强雍宜民四宮谦一首都医科大学宣武医院骨科北京市100053 中国华北电力集团公司北京电力医院骨科北京市100073 东京医科齿科大学医学部骨科中国医科大学第一临床学院骨科辽宁省沈阳市110001

目的:多孔生物材料中心部所存在的气泡有可能限制了细胞和培养液进入中心部,从而限制了材料内细胞的进一步增殖和分化,继而影响移植后的体内骨形成。实验设计使用低压方法排除多孔材料生物材料β-TCP内的气泡,检测细胞/β-TCP复合体的... 详细信息

目的:多孔生物材料中心部所存在的气泡有可能限制了细胞和培养液进入中心部,从而限制了材料内细胞的进一步增殖和分化,继而影响移植后的体内骨形成。实验设计使用低压方法排除多孔材料生物材料β-TCP内的气泡,检测细胞/β-TCP复合体的成骨能力,以验证其可行性。方法:实验于2003-04/2005-06在东京医科齿科大学医学部骨科实验室完成。在体外培养扩增骨髓间充质细胞后,将细胞置于成骨分化的培养液中,通过常压(大气压,101.1kPa)和低压(13.3kPa)两种方法将细胞灌注种植入72个多孔β-TCP人工骨中。细胞种植后检测β-TCP人工骨内含有的细胞悬浊液量,细胞生存率和细胞种植效率;应用扫描电镜观察细胞种植后人工骨内细胞附着情况。并在继续细胞分化培养1,2,3,4周时检测人工骨的DNA含量和碱性磷酸酶活性。结果:①低压组人工骨的细胞悬浊液量、全细胞种植效率和活细胞种植效率均高于常压组(P0.05)。②扫描电镜观察显示低压组人工骨内附着更多的骨髓间充质细胞。③低压组细胞分化培养过程中不同时点的人工骨碱性磷酸酶活性和DNA含量均高于常压组(P<0.01)。结论:13.3kPa低压细胞灌注法可以明显提高多孔人工骨的细胞种植和成骨能力。低压细胞种植法是一个既简单方便又实用有效的方法。

关键词：低压骨髓基质细胞骨形成磷酸三钙

Endoscopic approach through the minor papilla for the management of pancreatic diseases

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World Journal of Gastrointestinal Endoscopy 2013年第3期5卷 81-88页

作者： Nao Fujimori Hisato Igarashi Akira asou Ken Kawabe Lingaku Lee Takamasa Oono Taichi Nakamura Yusuke Niina Masayuki Hijioka Masahiko Uchida Kazuhiro Kotoh Kazuhiko Nakamura Tetsuhide Ito Ryoichi Takayanagi Department of Medicine and Bioregulatory Science Graduate School of Medical SciencesKyushu UniversityHigashi-kuFukuoka 812-8582Japan Department of Gastroenterology National Hospital OrganizationKyushu Medical CenterChuou-kuFukuoka 810-8563Japan

AIM:To clarify the efficacy and safety of an endoscopic approach through the minor papilla for the management of pancreatic ***:This study included 44 endoscopic retrograde cholangiopancreatography(ERCP) procedures performed in 34 patients using a minor papilla approach between April 2007 and March *** retrospectively evaluated the clinical profiles of the patients,the endoscopic interventions,short-term outcomes,and ***:Of 44 ERCPs,26 were diagnostic ERCP,and 18 were therapeutic *** most common cause of difficult access to the main pancreatic duct through the major papilla was pancreas divisum followed by distortion of Wirsung's *** overall success rate of minor papilla cannulation was 80%(35/44),which was significantly improved by wire-guided cannulation(P = 0.04).Endoscopic minor papillotomy(EMP) was performed in 17 of 34 patients(50%) using a needle-knife(13/17) or a pull-type papillotome(4/17).EMP with pancreatic stent placement,which was the main therapeutic option for patients with chronic pancreatitis,recurrent acute pancreatitis,and pancreatic pseudocyst,resulted in short-term clinical improvement in 83% of *** post-ERCP pancreatitis occurred as an early complication in 2 cases(4.5%).CONCLUSION:The endoscopic minor papilla approach is technically feasible,safe,and effective when the procedure is performed in a high-volume referral center by experienced endoscopists.

关键词： Endoscopic papillotomy Endoscopic retrograde cholangiopancreatography Minor papilla Pancreas divisum Pancreatitis