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Phylogenetic Relationships of Japanese Unionoida (Mollusca: Bivalvia) Based on Mitochondrial 16S rDNA Sequences

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Journal of Water Resource and Protection 2017年第5期9卷 493-509页

作者： Isao Sano Akihisa Shirai Takaki Kondo Jun-Ichi miyazaki Faculty of Education University of Yamanashi Yamanashi Japan Musashi High School and Junior High School Tokyo Japan Division of Natural Science Osaka Kyoiku University Osaka Japan

Japanese unionoid mussels are classified into 2 families (Margaritiferidae and Unionidae), 12 genera, and 18 species based on the morphological characteristics of both adults and larvae;however, there are some debates regarding their systematics. In this study, we determined mitochondrial 16S ribosomal DNA sequences (347-bp) for 60 specimens belonging to 18 species and constructed trees to elucidate phylogenetic relationships and evaluate the current systematics of Japanese unionoid mussels. Almost all species formed clades, except for Inversiunio yanagawensis, Sinanodonta lauta, S. japonica, and Margaritifera laevis, even though two or more specimens were collected from distant localities. All genera formed highly supported clades with the exception of the genus Sinanodonta. Phylogenetic relationships obtained in this study supported systematics based on morphological and larval traits. Therefore, the current phylogenetic relationships and systematics of Japanese unionoid mussels are stronger than they were before;now that they are corroborated by genetic data.

关键词： Systematics Molecular Barcoding Endangered Species Conservation East Asian Mussels

Influence of Previous Abdominal and Pelvic Surgeries on Uterine Artery Embolization

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Open Journal of Radiology 2023年第4期13卷 246-255页

作者： Yu Hasegawa Juliana Yumi Ishisaki Hitomi Kato Yoshiki Kuwatsuru Rie Ozaki Mari Kitade ryohei Kuwatsuru Department of Radiology Graduate School of Medicine Juntendo University Tokyo Japan Department of Radiology School of Medicine Juntendo University Tokyo Japan Department of Obstetrics and Gynecology School of Medicine Juntendo University Tokyo Japan Department of Obstetrics and Gynecology Graduate School of Medicine Juntendo University Tokyo Japan

Objective: To assess the influence of previous abdominal and pelvic surgeries on uterine artery embolization (UAE). We hypothesize that the formation of postoperative adhesions can impose technical difficulties for UAE, resulting in longer fluoroscopy time and less reduction of uterus and largest tumor volumes after the procedure. Methods: We retrospectively reviewed the medical records of 122 consecutive patients who underwent uterine artery embolization for the treatment of uterine fibroids in our department. Patients were divided into two groups according to the presence or absence of previous abdominal or pelvic surgery. Fluoroscopy time, change in symptoms, and volume variation of uterus and largest tumor were compared between groups. Results: Forty-seven patients underwent abdominopelvic surgery before UAE (Group 1) and 75 did not (Group 2). Median (IQR) fluoroscopy time was 22 minutes (IQR: 18 - 26.4) for Group 1, and 23.3 minutes (IQR: 19.05 - 28.25) for Group 2. No difference was found between the groups (P = 0.17). Regarding the results after UAE, neither the change in symptoms (P = 0.30) nor the volume variation for uterus (P = 0.41) and largest fibroid (P = 0.18) showed significant difference. Conclusions: In this study, previous abdominopelvic surgeries had no significant influence on fluoroscopy time, and overall outcomes of uterine artery embolization.

关键词： Female Uterine Artery Embolization Leiomyoma Uterus Fluoroscopy

Local advanced rectal cancer perforation in the midst of preoperative chemoradiotherapy:A case report and literature review

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World Journal of Clinical Cases 2017年第1期5卷 18-23页

作者： Nobuhisa Takase Kimihiro Yamashita Yasuo Sumi Hiroshi Hasegawa Masashi Yamamoto Shingo Kanaji Yoshiko Matsuda Takeru Matsuda Taro Oshikiri Tetsu Nakamura Satoshi Suzuki Yu-Ichiro Koma Masato Komatsu ryohei Sasaki Yoshihiro Kakeji Division of Gastrointestinal Surgery Department of Surgery Kobe University Graduate School of Medicine Division of Pathology Department of Pathology Kobe University Graduate School of Medicine Division of Diagnostic Pathology Department of Pathology Kobe University Graduate School of Medicine Department of Surgery Hyogo Cancer Center Department of Radiation Oncology Kobe University Graduate School of Medicine

Standard chemoradiotherapy(CRT) for local advanced rectal cancer(LARC) rarely induce rectal perforation. Here we report a rare case of rectal perforation in a patient with LARC in the midst of preoperative CRT. A 56-year-old male was conveyed to our hospital exhibiting general malaise. Colonoscopy and imaging tests resulted in a clinical diagnosis of LARC with direct invasion to adjacent organs and regional lymphadenopathy. Preoperative 5-fluorouracil-based CRT was started. At 25 d after the start of CRT, the patient developed a typical fever. Computed tomography revealed rectal perforation, and he underwent emergency sigmoid colostomy. At 12 d after the surgery, the remaining CRT was completed according to the original plan. The histopathological findings after radical operation revealed a wide field of tumor necrosis and fibrosis without lymph node metastasis. We share this case as important evidence for the treatment of LARC perforation in the midst of preoperative CRT.

关键词： Local advanced rectal cancer Preoperative chemoradiotherapy Rectal perforation 5-fluorouracil Tumor necrosis

Slim Water Injection Nozzle for Silicon Wafer Wet Cleaning Bath

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Advances in Chemical Engineering and Science 2016年第4期6卷 345-354页

作者： Shogo Okuyama Kento miyazaki Nobutaka Ono Hitoshi Habuka Akihiro Goto Department of Chemical and Energy Engineering Yokohama National University Yokohama Japan Shizuoka Factory Pre-Tech Co. Ltd. Yaizu Japan

In order to effectively and quickly clean the surface of semiconductor silicon wafers, the fluid flow is one of the significant issues. For a batch-type silicon wafer wet cleaning bath, a slim water injection nozzle consisting of a dual tube was studied, based on theoretical calculations and experiments. A thin inner tube was placed at the optimum position in the water injection nozzle. Such a simple design could make the water injection direction normal and the water velocity profile symmetrical along the nozzle. The water flow in the wet cleaning bath was observed using a blue-colored ink tracer. When the nozzle developed in this study was placed at the bottom of the bath, a fast and symmetrical upward water stream was formed between and around the wafers.

关键词： Silicon Wafer Wet Cleaning Bath Water Injection Nozzle Water Flow

Prenatal incarceration of caput succedaneum: A case report

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World Journal of Obstetrics and Gynecology 2013年第2期2卷 34-36页

作者： Atsuko Okazaki Ken miyazaki Kana Kihira Madoka Furuhashi Department of Obstetrics and Gynecology Japanese Red Cross Nagoya Daiichi HospitalNagoya 453-8511Japan

Caput succedaneum is relatively common at birth but infrequently diagnosed in utero. We report the first case of a prenatal incarcerated caput succedaneum after cervical cerclage in a patient with premature rupture of the membranes(PPROM). A 41-year-old woman was referred and admitted to our hospital due to PPROM at 19 wk of gestation. Aggressive therapy, including amnioinfusion, cervical cerclage, and administration of antibiotics and tocolysis, was initiated. At 24 wk of gestation, a thumb tip-sized and polyp-like mass, which was irreducible, was delineated with a vaginal examination, vaginal speculum, and transvaginal ultrasonography, leading to the diagnosis of incarcerated caput succedaneum. Under general anesthesia, the incarcerated caput succedaneum was repositioned with fingers after cutting the string to avoid necrosis, and then, placement of a Mc Donald cervical cerclage was undertaken again. At 26 wk of gestation, she delivered a 678 g girl through an emergency cesarean section performed due to profuse bleeding and prolonged decelerations. A slight bulge with hair was observed on the head by palpation at birth. Cephalic ultrasonography, X-ray, magnetic resonance imaging and electroencephalogram confirmed no abnormality. Although the baby needed oxygen(0.2 L/min) at the time of hospital discharge, she has grown favorably at three years of corrected age.

关键词： Caput succedaneum Cervical cerclage Patient with premature rupture of the membranes Ultrasonography Prenatal

Coordination and Photoisomerization of Azobenzene-Amino Acid Schiff Base Copper(II) Complexes to Lysozyme

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Journal of Materials Science and Chemical Engineering 2023年第3期11卷 34-44页

作者： Yuto Kuroda Ryotaro miyazaki Daiki Shimonishi Daisuke Nakane Takashiro Akitsu Department of Chemistry Faculty of Science Tokyo University of Science Tokyo Japan

In this study, we exhibited an amino acid (arginine and threonine) derivative Schiff base copper(II) complexes incorporating an azobenzene moiety as a photoresponsive site and conjugated it to egg white lysozyme, a well-known protein, to change ligand conformation under binding to lysozyme. Among several spectroscopic investigations, ESR clearly showed that the nitrogen atom of the amino acid residue of lysozyme was bound to the paramagnetic copper(II) ion of the complex, and UV light irradiation confirmed photoisomerization of the azobenzene moiety of the ligand to cis-form. The binding mode was considered by means of spectroscopic as well as computational methods, whereas complete crystallographic verification was still a preliminary stage.

关键词： Azobenzene Schiff Base Copper Amino Acid Lysozyme Artificial Metalloprotein

Clinical prediction of complicated appendicitis:A case-control study utilizing logistic regression

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World Journal of Clinical Cases 2020年第11期8卷 2127-2136页

作者： Yosuke Sasaki Fumiya Komatsu Naoyasu Kashima Takeshi Suzuki Ikutaka Takemoto Sho Kijima Tadashi Maeda Taito miyazaki Yoshiko Honda Hiroaki Zai Nagato Shimada Kimihiko Funahashi Yoshihisa Urita Department of General Medicine and Emergency Care Toho University School of MedicineOta-kuTokyo 143-8541Japan Department of General and Gastroenterological Surgery Toho University Omori Medical CenterOtakuTokyo 143-8541Japan

BACKGROUND Since high-quality evidence on conservative treatment of acute appendicitis using antibiotics has increased,differentiation of patients with complicated appendicitis(CA)from those with simple appendicitis(SA)has become increasingly *** studies have revealed that male gender,advanced age,comorbid conditions,prehospital delay,fever,and anorexia are risk factors of perforated *** serum C-reactive protein(CRP)level and hyponatremia have also been reported as predictive biomarkers of ***,confounding between various factors is problematic because most previous studies were limited to univariate *** To evaluate non-laboratory and laboratory predictive factors of CA using logistic regression *** We performed an exploratory,single-center,retrospective case-control study that evaluated 198 patients(83.9%)with SA and 38 patients(16.1%)with *** were confirmed by computed tomography images for all *** compared age,sex,onset-to-visit interval,epigastric/periumbilical pain,right lower quadrant pain,nausea/vomiting,diarrhea,anorexia,medical history(of previous non-surgically treated appendicitis,diabetes,hypertension,dyslipidemia,liver cirrhosis,hemodialysis,chronic lung diseases,malignant tumors,immunosuppressant use,and antiplatelet use),vital signs,physical findings,and laboratory data to select the explanatory variates for logistic *** on the univariate comparisons,we performed logistic regression for clinical differentiation between CA and SA using only nonlaboratory factors and also including both non-laboratory and laboratory *** The 236 eligible patients consisted of 198 patients(83.9%)with SA and 38 patients(16.1%)with *** median ages were 34 years old[interquartile ranges(IR),24-45 years]in the SA group and 49 years old(IR,35-63 years)in the CA group(P<0.001).The median onset-to-visit interval was 1 d(IR,0-1)and 1 d(IR,1-2)in the SA and CA groups,respectively

关键词： Acute abdomen Abscess Appendicitis C-reactive protein Forecasting Intestinal perforation

Markerless Respiratory Motion Tracking Using Single Depth Camera

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Open Journal of Medical Imaging 2016年第1期6卷 20-31页

作者： Shinobu Kumagai ryohei Uemura Toru Ishibashi Susumu Nakabayashi Norikazu Arai Takenori Kobayashi Jun’ichi Kotoku Graduate School of Medical Technology Teikyo University Tokyo Japan Central of Radiology Teikyo University Hospital Tokyo Japan

The aim of this study is to propose a novel system that has an ability to detect intra-fractional motion during radiotherapy treatment in real-time using three-dimensional surface taken by a depth camera, Microsoft Kinect v1. Our approach introduces three new aspects for three-dimensional surface tracking in radiotherapy treatment. The first aspect is a new algorithm for noise reduction of depth values. Ueda’s algorithm was implemented and enabling a fast least square regression of depth values. The second aspect is an application for detection of patient’s motion at multiple points in thracoabdominal regions. The third aspect is an estimation of three-dimensional surface from multiple depth values. For evaluation of noise reduction by Ueda’s algorithm, two respiratory patterns are measured by the Kinect as well as a laser range meter. The resulting cross correlation coefficients between the laser range meter and the Kinect were 0.982 for abdominal respiration and 0.995 for breath holding. Moreover, the mean cross correlation coefficients between the signals of our system and the signals of Anzai with respect to participant’s respiratory motion were 0.90 for thoracic respiration and 0.93 for abdominal respiration, respectively. These results proved that the performance of the developed system was comparable to existing motion monitoring devices. Reconstruction of three-dimensional surface also enabled us to detect the irregular motion and breathing arrest by comparing the averaged depth with predefined threshold values.

关键词： Depth Camera Markerless Motion Tracking Intra-Fractional Motion Three-Dimensional Surface

Quantitative CT Indexes and CT Visual Score in Interstitial Lung Abnormality as Indicators of Concurrent Lung Cancer

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Open Journal of Radiology 2019年第2期9卷 105-114页

作者： Akihiro Hotta Kazuhiro Suzuki Mariko Fukui Katsutoshi Ando Kenji Suzuki ryohei Kuwatsuru Kazuya Takamochi Departments of Radiology Faculty of Medicine Juntendo University Tokyo Japan Departments of Thoracic Surgery Faculty of Medicine Juntendo University Tokyo Japan Departments of Respiratory Medicine Faculty of Medicine Juntendo University Tokyo Japan

Background: The associations between the severity of interstitial lung disease and the stage and histologic type of concurrent lung cancer remain unknown. Purpose: To evaluate whether the severity of interstitial lung abnormality (ILA), as indicated by quantitative computed tomographic (CT) indexes and CT visual score, was correlated with the stage and histological type of concurrent lung cancer. Materials and Methods: Twenty-eight patients with surgically diagnosed lung cancer and ILA on CT were enrolled in this retrospective study. The subjects were allocated to one of three groups by histological type: adenocarcinoma group (13 subjects);squamous cell carcinoma group (10 subjects);and the other histological diagnosis group (5 subjects). Two independent observers evaluated the CT findings to determine the CT visual score, and the kurtosis and skewness of CT-based density histograms were determined. The relationships between severity of ILA and the pathological stage and histological type of concurrent lung cancer were evaluated. Results: There were no significant differences in the CT visual scores and quantitative indexes among the three groups. CT visual score was significantly negatively correlated with pathological stage (r = −0.43, P = 0.025). Conclusion: Patients can have only mild ILA on visual scoring but advanced lung cancer. Therefore, the frequency of follow-up examination should not be based on the severity of ILA on CT.

关键词： CT Visual Score Interstitial Lung Disease Lung Cancer Quantitative CT Index