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Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting

Horticulture Research 2023年第6期10卷 70-81页

作者： Chaokun Huang Ken-ichi Kurotani ryo tabata Nobutaka Mitsuda ryohei Sugita Keitaro Tanoi Michitaka Notaguchi Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology(AIST)TsukubaIbaraki 305-8566Japan Isotope Facility for Agricultural Education and Research Graduate School of Agricultural and Life SciencesThe University of TokyoYayoiBunkyo-kuTokyo 113-8657Japan Radioisotope Research Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8602Japan Institute of Transformative Bio-Molecules Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan

Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of *** this study,we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft *** and gene network analyses revealed gene modules for tracheary element(TE)formation during grafting that include genes associated with xylem cell differentiation and immune *** reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE(NbXCP)genes in TE formation during interfamily *** activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft *** of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft ***,grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit ***,we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.

关键词： grafting element graft

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Three-dimensional crystal defect imaging by STEM depth sectioning

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Chinese Physics B 2024年第8期33卷 2-7页

作者： ryo Ishikawa Naoya Shibata and Yuichi Ikuhara Institute of Engineering Innovation The University of TokyoBunkyoTokyo 113-8656Japan

One of the major innovations awaiting in electron microscopy is full three-dimensional imaging at atomic *** the success of aberration correction to deep sub-angstrom lateral resolution,spatial resolution in depth is still far from atomic *** scanning transmission electron microscopy(STEM),this poor depth resolution is due to the limitation of the illumination *** overcome this physical limitation,it is essential to implement a next-generation aberration corrector in STEM that can significantly improve the depth *** review discusses the capability of depth sectioning for three-dimensional imaging combined with large-angle illumination ***,the statistical analysis approach remarkably improves the depth resolution,making it possible to achieve three-dimensional atomic resolution imaging at oxide *** will also discuss the future prospects of three-dimensional imaging at atomic resolution by STEM depth sectioning.

关键词： atomic-resolution STEM STEM depth sectioning depth resolution dopants surface topography

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Fabrication of Mie-resonant silicon nanoparticles using laser annealing for surface-enhanced fluorescence spectroscopy

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Microsystems & Nanoengineering 2024年第2期10卷 133-141页

作者： Tatsuya Fukuta ryo Kato Takuo Tanaka Taka-aki Yano Institute of Post-LED Photonics Tokushima University2-1 Minami-JosanjimaTokushima770-8506Japan Innovative Photon Manipulation Research Team RIKEN Center for Advanced PhotonicsWakoSaitama351-0198Japan Metamaterials Laboratory RIKEN Cluster for Pioneering ResearchWakoSaitama351-0109Japan

Silicon nanostructures with unique Mie resonances have garnered considerable attention in the field of ***,we present a simple and efficient method for the fabrication of silicon(Si)nanoparticle substrates using continuous-wave(CW)laser *** resulting silicon nanoparticles exhibit Mie resonances in the visible region,and their resonant wavelengths can be precisely ***,laser-annealed silicon nanoparticle substrates show a 60-fold enhancement in *** tunable and fluorescence-enhancing silicon nanoparticle platform has tremendous potential for highly sensitive fluorescence sensing and biomedical imaging applications.

关键词： nanoparticles annealing resonant

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BEACH-Domain Proteins Act Together in a Cascade to Mediate Vacuolar Protein Trafficking and Disease Resistance in Arabidopsis

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Molecular Plant 2015年第3期8卷 389-398页

作者： Ooi-kock Teh Noriyuki Hatsugai Kentaro Tamura Kentaro Fuji ryo tabata Katsushi Yamaguch Shuji Shingenobu Masashi Yamada Mitsuyasu Hasebe Shinichiro Sawa Tomoo Shimada Ikuko Hara-Nishimura Graduate School of Science Kyoto University Sakyo-ku Kyoto 606-8502 Japan Research Centre for Cooperative Projects Hokkaido University Kita-ku Sapporo 060-8638 Japan Graduate School of Science and Technology Kumamoto University Kumamoto 860-8555 Japan Functional Genomics Facility National Institute for Basic Biology Okazaki 444-8585 Japan Department of Biology and IGSP Center for Systems Biology Duke University Durham NC 27708 USA Division of Evolutionary Biology National Institute for Basic Biology Okazaki 444-8585 Japan School of Life Science The Graduate University for Advanced Studies Okazaki 444-8585 Japan Present address: Department of Plant Biology and Forest Genetics Uppsala BioCenter Swedish University of Agricultural Sciences (SLU) and Linnean Centre ofPlant Biology Uppsala SE-75007 Sweden present address: Microbial and Plant Genomics Institute University of Minnesota St Paul MN 55108 USA Present address: RIKEN Innovation Center RIKEN 2-1 Hirosawa Wako Saitama 351-0198 Japan

Membrane trafficking to the protein storage vacuole （PSV） is a specialized process in seed plants. However, this trafficking mechanism to PSV is poorly understood. Here, we show that three types of Beige and Chediak-Higashi （BEACH）-domain proteins contribute to both vacuolar protein transport and effector- triggered immunity （ETI）. We screened a green fluorescent seed （GFS） library of Arabidopsis mutants with defects in vesicle trafficking and isolated two allelic mutants gfs3 and gfs12 with a defect in seed pro- tein transport to PSV. The gene responsible for the mutant phenotype was found to encode a putative pro- tein belonging to group D of BEACH-domain proteins, which possess kinase domains. Disruption of other BEACH-encoding loci in the gfs12 mutant showed that BEACH homologs acted in a cascading manner for PSV trafficking. The epistatic genetic interactions observed among BEACH homologs were also found in the ETI responses of the gfs12 and gfs12 bchb-1 mutants, which showed elevated avirulent bacterial growth. The GFS12 kinase domain interacted specifically with the pleckstrin homology domain of BchCl. These results suggest that a cascade of multiple BEACH-domain proteins contributes to vacuolar protein transport and plant defense.

关键词： BEACH-domain protein Arabidopsis thaliana vacuolar protein transport protein storage vacuoles,plant immunity

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α,β-Dehydroamino Acid Synthesis through Proline-Catalyzed Aldol Condensation with a Glycine Schiff Base

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Precision Chemistry 2024年第1期2卷 14-20页

作者： Jun Sawamura Daikan Ieiri ryo Yazaki Takashi Ohshima Graduate School of Pharmaceutical Sciences Kyushu UniversityFukuoka 812-8582Japan

A general protocol for the synthesis ofα,β-dehydroamino acids and their peptides was *** efficiently catalyzed an aldol condensation reaction of a glycine Schiff base with a variety of *** hydroxy group on the benzophenone imine was crucial for high Z/E selectivity and further transimination for protecting group-freeα,β-dehydroamino *** elongation of both the C-and N-terminals highlighted the usefulness of our present protocol.

关键词： α β-dehydroamino acid peptide proline aldol condensation glycine Schiff base transimination

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In situ observation of the phase transformation kinetics of bismuth during shock release

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Chinese Physics B 2024年第4期33卷 586-592页

作者：李江涛王倩男徐亮柳雷张航 Sota Takagi Kouhei Ichiyanagi ryo Fukaya Shunsuke Nozawa 胡建波 Laboratory for Shock Wave and Detonation Physics Research Institute of Fluid PhysicsChina Academy of Engineering PhysicsMianyang 621900China State Key Laboratory for Environment-Friendly Energy Materials Southwest University of Science and TechnologyMianyang 621010China Japan Society for the Promotion of Science Tokyo102-0083Japan Institute of Materials Structure Science High Energy Accelerator Research OrganizationIbaraki305-8572Japan

A time-resolved x-ray diffraction technique is employed to monitor the structural transformation of laser-shocked *** reveal a retarded transformation from the shock-induced Bi-Ⅴphase to a metastable Bi-Ⅳphase during the shock release,instead of the thermodynamically stable Bi-Ⅲ*** emergence of the metastable Bi-Ⅳphase is understood by the competitive interplay between two transformation pathways towards the Bi-Ⅳand Bi-Ⅲ,*** former is more rapid than the latter because the Bi-Ⅴto B-Ⅳtransformation is driven by interaction between the closest atoms while the Bi-Ⅴto B-Ⅲtransformation requires interaction between the second-closest *** nucleation time for the Bi-Ⅴto Bi-Ⅳtransformation is determined to be 5.1±0.9 ns according to a classical nucleation *** observation demonstrates the importance of the formation of the transient metastable phases,which can change the phase transformation pathway in a dynamic process.

关键词： phase transformation time-resolved x-ray diffraction(XRD) bismuth metastable phase nonequilibrium phase diagram

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Operationally Simple Enantioselective Silane Reduction of Ketones by the [Ir(OMe)(cod)]₂/Azolium Catalytic System

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International Journal of Organic Chemistry 2024年第1期14卷 1-19页

作者： Satoshi Sakaguchi Chika Nagao ryo Ichihara Shogo Matsuo Department of Chemistry and Materials Engineering Faculty of Chemistry Materials and Bioengineering Kansai University Suita Japan

An operationally simple protocol was designed for the enantioselective silane reduction (ESR) of ketones using air- and moisture-stable [Ir(OMe)(cod)]2 (cod = 1,5-cyclooctadiene) (3) as a metal catalyst precursor. This reaction was driven by chiral hydroxyamide-functionalized azolium salt 2. The catalytic ESR reaction could be performed under benchtop conditions at room temperature. Treatment of 2 with 3 in THF yielded the monodentate IrCl(NHC)(cod) (NHC = N-heterocyclic carbene) complex 4 in 93% yield, herein the anionic methoxy ligand of 3 serves as an internal base that deprotonates the azolium ring of 2. The well-defined Ir complex 4 catalyzed the ESR reaction of propiophenone (6) with (EtO)2MeSiH using the pre-mixing reaction procedure. Based on this success, the catalytic ESR reaction was designed and implemented using an in situ-generated NHC/Ir catalyst derived from 2 and 3. Thus, a wide variety of aryl ketones could be reduced to the corresponding optically active alcohols in moderate to excellent stereoselectivities at room temperature without temperature control. Since the high catalytic activity of 3 was observed, we next evaluated several other transition metal catalyst precursors for the catalytic ESR reaction under the influence of 2. This evaluation revealed that Ir(acac)(cod) (acac = acetylacetonate) (28) and [IrCl(cod)]2 (5) can be successfully used as metal catalyst precursors in the ESR reaction.

关键词： Asymmetric Catalysis Enantioselective Reduction Hydrosilylation Reaction N-Heterocyclic Carbene Iridium

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Stresses in the Scapular Fossa Do Not Exceed the Yield Stress When Elevated up to 135 Degrees of Abduction after Reverse Shoulder Arthroplasty

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Journal of Biomedical Science and Engineering 2024年第2期17卷 35-40页

作者： Rina Sakai Tomonori Kenmoku ryo Tazawa Kazuhiro Yoshida Tomomi Mizuhashi Masanobu Ujihira Department of Medical Engineering and Technology Kitasato University School of Allied Health Sciences Sagamihara Japan Department of Orthopedic Surgery Kitasato University School of Medicine Sagamihara Japan Kitasato University Graduate School of Medical Sciences Sagamihara Japan

Reverse shoulder arthroplasty (RSA) is an effective treatment for rotator cuff tears. Despite its advantages, complications occur at a high rate. Complications requiring revision include a high rate of base plate failure, 38% of which are due to instability. The primary stability the base plate ensures is a crucial factor and, thus, is the subject of much debate in clinical studies and biomechanical research. This study is aimed to provide data that will contribute to the base plate’s pri-mary stability and glenoid longevity by clarifying the stresses at the scapular fossa and base plate interface associated with elevation after RSA. A 3D finite element model was created from the DICOM data for the scapulohumeral joint and SMR shoulder system. For loading conditions, 30 N was applied for each posi-tion with abduction angles of 0, 45, 90, and 135 degrees. A three-dimensional fi-nite element analysis was performed using the static implicit method with LS-DYNA. The von Mises stresses in the scapular fossa were found not to exceed the yield stress on the bone even after elevation to an abduction angle of 135 de-grees after RSA. It is rough to uniformly compare the yield stress and the von Mises stress, but it was inferred that the possibility of fracture is low unless a large external force is applied. A maximum von Mises stress showed 0 degrees of abduction, suggesting that the lowered position is in a more severe condition than the elevated position. If better improvement is desired, it may be necessary to devise ways to reduce the stress on the upper screw. .

关键词： Reverse Shoulder Arthroplasty Finite Element Analysis Yield Stress Glenoid

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Swimmer with submerged SiO_(2)/Al/LiNbO surface acoustic wave propulsion system

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Biomimetic Intelligence & Robotics 2024年第2期4卷 71-77页

作者： Deqing Kong ryo Tanimura Fang Wang Kailiang Zhang Minoru Kuribayashi Kurosawa Manabu Aoyagi Department of Engineering Muroran Institute of TechnologyMuroran 050-8585Japan Tianjin Key Laboratory of Film Electronic and Communication Devices School of Integrated Circuit Science and EngineeringTianjin University of TechnologyTianjin 300384China School of Engineering Tokyo Institute of TechnologyYokohama 226-8501Japan

Acoustic propulsion system presents a novel underwater propulsion approach in small scale *** study introduces a submerged surface acoustic wave(SAW)propulsion system based on the SiO_(2)/Al/LiNbO_(3) *** 19.25 MHz,the SAW propulsion system is proposed and investigated by the propulsion force calculation,PIV measurements and propulsion measurements.3.3 mN propulsion force is measured at 27.6 *** evaluate the miniature swimmer,the SAW propulsion systems with multiple frequencies are *** 2.2 W,the submerged SAW propulsion system at 38.45 MHz demonstrates 0.83 mN/mm^(2) propulsion *** 96.13 MHz and 24 Vpp,the movements of miniature swimmer with a fully submerged SAW propulsion system are recorded and analyzed to a maximum of 177 mm/*** of miniaturization,high power density,and simple structure,the SAW propulsion system can be expected for some microrobot applications,such as underwater drone,pipelinerobotand intravascularrobot.

关键词： Acoustofluidics Surface acoustic wave Swimmer Underwater propulsion system

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语言的运动、镜头的单纯性——今泉力哉论

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北京电影学院学报 2024年第2期 76-87页

作者： [日]坚田谅朱依拉(译) 北海道大学大学院文学院浙江传媒学院电视艺术学院

本论文讨论的,是在当代日本电影界中以惊人的速度进行电影创作的今泉力哉的电影作品。回顾电影批评与评论的历史,有些评论家认为电影的本质在于影像与动作,而这些评论家们往往倾向于认为台词与语言不如影像与动作。尽管今泉力哉的电影... 详细信息

本论文讨论的,是在当代日本电影界中以惊人的速度进行电影创作的今泉力哉的电影作品。回顾电影批评与评论的历史,有些评论家认为电影的本质在于影像与动作,而这些评论家们往往倾向于认为台词与语言不如影像与动作。尽管今泉力哉的电影有时被描述为“心理性”的,但本文将揭示今泉力哉电影中的台词(语言)包含着不局限于心理性的方面这一事实。此外,在今天这个数字电影制作的时代,过去那种比起台词与语言,更倾向于将影像与动作视作电影本质的观点已经不再适用,本文将阐明今泉力哉作为一位探究影像与语言的新关系的电影创作者的存在。

关键词：今泉力哉台词语言镜头数字电影制作

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