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Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

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Chinese Science Bulletin 2009年第19期54卷 3536-3545页

作者： ZHOU GuoAn GUAN RongXia LI YingHui chang ruzhen QIU LiJuan National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers

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Chinese Science Bulletin 2006年第9期51卷 1078-1084页

作者： QIN Jun CHEN Weiyuan GUAN Rongxia JIANG Chengxi LI Yinghui FU Yashu LIU Zhangxiong ZHANG Mengchen chang ruzhen QIU Lijuan National Key Facility of Crop Gene Resources and Genetic Improvement Key Lab of Crop Gerrnplasm Resources & Biotechology (MOA) Institute of Crop Science Chinese Agricultural Academy of Sciences Beijing 100081 China Hebei Academy of Agricultural and Forestry Science Shijiazhuang 050031 China Heilongjiang Academy of Agricultural Science Suihua 152052 China

Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25.

关键词：大豆谱系分析 SSR 序列分析种质资源

Geographical distribution of GmTfl1 alleles in Chinese soybean varieties

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The Crop Journal 2015年第5期3卷 371-378页

作者： Guifeng Liu Lin Zhao Benjamin J.Averitt Ying Liu Bo Zhang ruzhen chang Yansong Ma Xiaoyan Luan Rongxia Guan Lijuan Qiu Soybean Research Institute Northeast Agricultural University Department of Crop and Soil Environmental Sciences VA Polytechnic Institute and State University The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences Heilongjiang Academy of Agricultural Sciences

Stem growth habit is an important agronomic trait in soybean and is subject to artificial selection. This study aimed to provide a theory for genotypic selection of stem growth habit for breeding purposes by analyzing the alleles of Gm Tfl1 gene in Chinese soybean varieties and establishing a database of Gm Tfl1 variation. Using knowledge of insertion and deletion(Indel) in the non-coding region and four single-nucleotide polymorphisms(SNPs) in the coding sequences of the Gm Tfl1 gene, four CAPS and one Indel markers were developed and used to test 1120 Chinese soybean varieties. We found that the dominant Gm Tfl1 allele was prevalent in accessions from the Northern ecoregion, whereas the recessive allele, Gmtfl1, was more common in the Southern ecoregion, and the proportions of Gm Tfl1 and recessive alleles were respectively 40.1% and 59.9% in the Huang-Huai ecoregion. The proportion of Gm Tfl1 decreased and that of Gmtfl1 increased, gradually from north to south. Allele Gm Tfl1-a was present in higher proportions in the Huang-Huai spring, Huang-Huai summer, and Northern spring sub-ecoregions than that in the other sub-ecoregions. Gm Tfl1-b was common in the Northeast spring, Northern spring and Southern summer sub-ecoregions. Gmtfl1-ta was found mainly in the Huang-Huai spring,Huang-Huai summer and Southern spring sub-ecoregions. The Gmtfl1-ab allele was distributed in all six soybean sub-ecoregions. The Gmtfl1-bb allele was distributed mainly in the Huang-Huai spring and summer and Southern spring and summer sub-ecoregions,but the Gmtfl1-tb allele was detected only in the Huang-Huai summer sub-ecoregion. The distributions of Gm Tfl1 and Gmtfl1 have shown no large changes in nearly 60 years of breeding, but the frequency of the recessive genotype Gmtfl1 has shown a rising trend in the last 20 years. This study provides a theoretical foundation for breeding new soybean varieties for different ecoregions.

关键词： Soybean Stem growth habit Gm Tfl1 Distribution

Mapping and validation of a dominant salt tolerance gene in the cultivated soybean(Glycine max) variety Tiefeng 8

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The Crop Journal 2014年第6期2卷 358-365页

作者： Rongxia Guan Jiangang Chen Jinghan Jiang Guangyu Liu Ying Liu Lei Tian Lili Yu ruzhen chang Li-juan Qiu The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Key Laboratory of Germplasm & Biotechnology (MOA)Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng *** objective of the present study was to genetically map the salt-tolerance gene in an F2:3population and a recombinant inbred line(RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8(tolerant) and 85-140(sensitive). The F2:3families and RILs were treated with 200 mmol L-1Na Cl to evaluate salt tolerance. The F2:3population showed 1(42 tolerant): 2(132 segregating): 1(65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region(SCAR) marker from a previously identified random amplified polymorphic DNA(RAPD)marker and four insertion/deletion polymorphism(In Del) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_3_1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean.

关键词： Salt tolerance Gene mapping Soybean Glycine max

Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha

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The Crop Journal 2017年第4期5卷 326-336页

作者： Zhangxiong Liu Jun Li Xuhong Fan Nang Myint Phyu Sin Htwe Shuming Wang Wen Huang Jiyu Yang Lili Xing Lijun Chen Yinghui Li Rongxia Guan ruzhen chang Dechun Wang Lijuan Qiu National Key Facility for Gene Resources and Genetic Improvement Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop Science Chinese Academy of Agricultural Science Beijing 100081 China College of Agriculture Guangxi University Nanning 530004 China Institute of Soybean Research Jilin Academy of Agricultural Sciences Changchun 130124 China Tonghua Academy of Agricultural Sciences Meihekou 135007 Jilin China Jilin City Academy of Agricultural Sciences Jilin 132101 China Beijing Seed Administration Station Beijing 100088 China Department of Plant Soil and Microbial Sciences Michigan State University East Lansing 48824 USA

The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of *** conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation *** the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental *** molecular IDs of the 138 accessions were constructed using the core 20 *** is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic *** this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory *** high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources.

关键词： Soybean SNP Genetic diversity Genetic distance Molecular ID

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历史的回顾——大豆学术交流

大豆科技 2019年第5期 1-3页

作者：常汝镇中国农业科学院作物科学研究所中国农业科学院作物科学研究所

1963年8月26日至9月3日,中国作物学会在江苏徐州召开了全国豆类作物学术讨论会,出席会议的有47位代表,收到论文42篇,会后编印了论文集,王金陵、张子金、费家骍、王国勋等大豆知名专家出席会议。会议期间除学术交流活动,重点讨论了黄淮... 详细信息

1963年8月26日至9月3日,中国作物学会在江苏徐州召开了全国豆类作物学术讨论会,出席会议的有47位代表,收到论文42篇,会后编印了论文集,王金陵、张子金、费家骍、王国勋等大豆知名专家出席会议。会议期间除学术交流活动,重点讨论了黄淮地区夏大豆生产。当时黄淮地区大豆面积很大,最多时有7000多万亩,冬小麦收后主要种植夏大豆,当时玉米极少种植。

关键词：中国作物学会豆类作物夏大豆大豆面积王金陵学术讨论会学术交流江苏徐州

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我曾参加的有关发展大豆生产的调研和讨论

大豆科技 2019年第6期 1-3页

作者：常汝镇中国农业科学院作物科学研究所

我国大豆生产起伏较大,面积最大时达1.9亿亩(1957年),三年困难时期面积下降,1962年只有1.07亿亩,改革开放以后,大豆种植面积虽有起伏,但多数年份都在800~900万公顷,2004年大豆面积950.9万公顷,应是改革开放后面积最大的一年。但近些年... 详细信息

我国大豆生产起伏较大,面积最大时达1.9亿亩(1957年),三年困难时期面积下降,1962年只有1.07亿亩,改革开放以后,大豆种植面积虽有起伏,但多数年份都在800~900万公顷,2004年大豆面积950.9万公顷,应是改革开放后面积最大的一年。但近些年来由于玉米效益显著高于大豆,又由于早熟玉米杂交种的引进推广,玉米面积增加,挤压了大豆,2015年大豆面积低至610万公顷。这两年调整种植结构,大豆面积又缓慢增加。我从1971年参加“东北地区大豆生产调查”,到2012年参加“促进我国大豆生产稳定发展专题会议”,多次参加有关我国大豆生产的考察和研讨,见证过国家各级领导和部门对大豆生产的关心,在此仅把几次印象深刻的做个简单回顾。

关键词：大豆面积大豆生产玉米面积引进推广三年困难时期大豆种植面积玉米杂交种专题会议

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大豆胞囊线虫抗性基因定位与克隆研究进展

植物学通报 2006年第1期23卷 14-22页

作者：袁翠平常汝镇邱丽娟中国农业科学院作物科学研究所农作物基因资源与遗传改良重大科学工程北京100081

大豆胞囊线虫(soybeancystnematode,SCN)是大豆生产上一种危害严重的世界性害虫,能给大豆生产造成极大损失。大豆抗性品种选育是防治其措施中最经济、有效的方法。大豆SCN抗性的分子遗传学研究是开展大豆SCN抗性分子育种的理论基础,本... 详细信息

大豆胞囊线虫(soybeancystnematode,SCN)是大豆生产上一种危害严重的世界性害虫,能给大豆生产造成极大损失。大豆抗性品种选育是防治其措施中最经济、有效的方法。大豆SCN抗性的分子遗传学研究是开展大豆SCN抗性分子育种的理论基础,本文针对SCN抗性基因定位和克隆两个方面的研究现状进行了综述,并对当前研究中存在的问题及发展前景进行了讨论与展望。

关键词：大豆大豆胞囊线虫抗性基因遗传定位克隆

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2014年中国大豆基因资源发掘的主要进展

作物杂志 2015年第1期 1-5页

作者：邱丽娟郭勇常汝镇中国农业科学院作物科学研究所北京100081

中国大豆科学家2014年在基因资源发掘研究领域进展显著,尤其是在大豆泛基因组构建、基因剪切和结瘤基因等多个方面取得了原创性成果,发表了一批高水平的研究论文,部分研究成果已达到世界先进或领先水平。本文对2014年中国大豆基因资源... 详细信息

中国大豆科学家2014年在基因资源发掘研究领域进展显著,尤其是在大豆泛基因组构建、基因剪切和结瘤基因等多个方面取得了原创性成果,发表了一批高水平的研究论文,部分研究成果已达到世界先进或领先水平。本文对2014年中国大豆基因资源发掘相关研究取得的重要进展进行了概括性评述,旨在通过对聚焦成果的介绍反映当前中国大豆基础和应用基础领域的国际前沿和研究热点,同时展示我国科学家所取得的研究成果,为我国大豆基因资源的发掘与利用提供参考。

关键词：中国大豆基因发掘研究进展 2014