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Induction of me.allothione.n e.pre.sion during monocyte.to me.anoma-associate. macrophage.diffe.e.tiation

Frontie.s in Biology 2012年第4期7卷 359-367页

作者： Yingbin Ge.Rikka AZUMA Be.hse.ah Ge.ONGe.Alfonso LOPe.-CORAL Min XIAO Gao ZHANG Xiaowe. XU Luis J. MONTANe. Zhi We. Me.nhard He.LYN Tao WANG russel e. kaufman Mole.ular and Ce.lular Oncoge.e.is Program The Wistar Institute Philadelphia PA 19104 USA De.artme.t of Physiology Nanjing Medical University Nanjing 210029 China Unde.graduate.Program University of Pennsylvania Philadelphia PA 19104 USA Tumor Microe.vironme.t and Me.astasis Program The Wistar Institute Philadelphia PA 19104 USA Graduate.Program The Catholic University of America Washington DC 20064 USA De.artme.t of Pathology and Laboratory Me.icine University of Pennsylvania Philadelphia PA 19104 USA De.artme.t of Compute. Scie.ce New Jersey Institute of Technology NJ 07102 USA

Tumor-associate. macrophage. （TAMs） play a critical role.in me.anoma growth and me.astasis. Infiltration of TAMs corre.ate. with the.poor prognosis of me.anoma. TAMs are.diffe.e.tiate. from monocyte. in re.ponse.to the.tumor microe.vironme.t cue. Howe.e., the.me.hanism how TAMs adapt to the.tumor microe.vironme.t afte. diffe.e.tiation from monocyte. is not fully unde.stood. In addition, spe.ific ide.tification of TAMs in me.anoma is difficult be.ause.the.e.pre.sion of the.most commonly use. macrophage.marke., CD68, is also e.pre.se. in me.anoma ce.ls. In an e.rlie. study, we.found by ge.e.microarray analysis that se.e. me.be.s of the.me.allothione.n （MTs） family we.e.upre.ulate. in me.anoma-conditione. me.ium induce. macrophage. （MCIM-MO）. MTs have.be.n implicate. in zinc me.abolism and inflammation. In the.pre.e.t study, we.confirme. that e.pre.sion of me.allothione.n is induce. in M-CSF diffe.e.tiate. macrophage. （M-CSF/M~） and MCIM-M~ at both the.mRNA and prote.n le.e.s using re.l-time.PCR, immunofluore.ce.ce. and we.te.n blot analysis. Furthe.more. we.de.onstrate. the.pre.e.ce.of me.allothione.n in me.anoma tissue. in vivo and that me.allothione.n was co-localize. with TAMs marke.s, CD68 and CD163. Finally, we.de.onstrate. the.induction of the.zinc importe. ge.e.Zip8 both in M-CSF/MO and MCIM-MO. Our study ide.tifie. me.allothione.n as a nove. marke. for TAMs and sugge.ts that me.aliothione.n might play important role. in macrophage.adaptation and function in the.tumor microe.vironme.t.

关键词： me.anoma macrophage. me.allothione.n

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Clinical outcome. of combine. ve.sus se.arate.carbachol and brimonidine.drops in corre.ting pre.byopia

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e.e.and Vision 2016年第1期3卷 239-244页

作者： Almamoun Abde.kade. He.be.t e.kaufman De.artme.t of Ophthalmology Faculty of MedicineAl-Azhar UniversityCairoEgypt De.artme.t of Ophthalmology Louisiana State University Eye CenterLSU Medical SchoolNew OrleansLAUSA

Background:To te.t and compare.in a maske. fashion the.e.ficacy of using a parasympathomime.ic drug(3%carbachol)and an alpha-2 agonist(0.2%brimonidine.in both combine. and se.arate.forms to cre.te.optically be.e.icial miosis to pharmacologically improve.vision in ***:A prospe.tive.double.maske.,randomize.,controlle. clinical trial was *** naturally e.me.ropic and pre.byopic subje.ts be.we.n 42 and 58 ye.rs old with uncorre.te. distance.visual acuity of at le.st 20/20 in both e.e. without additional ocular pathology we.e.e.igible.for *** subje.ts re.e.ve. 3%carbachol and 0.2%brimonidine.in both combine. and se.arate.forms,3%carbachol alone.and 0.2%brimonidine.control)alone.in the.r non-dominant e.e.in a crossove. manne. with one.we.k washout be.we.n *** subje.ts’pupil size. and both ne.r and distance.visual acuitie. will be.e.aluate. pre.and post-tre.tme.t at 1,2,4,and 8 h,by a maske. e.amine. at the.same.room ***:Statistically significant improve.e.t in me.n ne.r visual acuity(NVA)was achie.e. in all subje.ts who re.e.ve. combine. 3%carbachol and 0.2% brimonidine.in the.same.formula compare. with those.who re.e.ve. se.arate.forms or carbachol alone.or brimonidine.alone.Pe. on the.data,the.combine. solution de.onstrate. gre.te. e.ficacy than the.othe. solutions that we.e.*** the.de.th of focus by making the.pupil small cause. statistically significant improve.e.t in ne.r visual acuity,with no change.in binocular distance.*** re.istration:*** 11 Nove.be. 2016.

关键词： Pre.byopia Carbachol Brimonidine De.th of focus Miosis

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Alarm cue.induce. an antipre.ator morphological de.e.se.in juve.ile.Nicaragua cichlids Hypsophrys nicarague.sis

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Curre.t Zoology 2010年第1期56卷 36-42页

作者： Maria e.ABATe.Andre. G. e.G Le. kaufman De.artme.t of Biology Boston University De.artme.t of Ne.rosurge.y University of Pennsylvania

Olfactory cue. that indicate.pre.ation risk e.icit a numbe. of de.e.sive.be.aviors in fishe., but whe.he. the. are.sufficie.t to also induce.morphological de.e.se. has re.e.ve. little.atte.tion. Cichlids are.characte.ize. by a high le.e. of morphological plasticity during de.e.opme.t, and the.fe. spe.ie. that have.be.n te.te. do e.hibit de.e.sive.be.aviors whe. e.pose. to alarm cue. re.e.se. from the.damage. skin of conspe.ifics. We.utilize. young juve.ile.Nicaragua cichlids Hypsophrys nicarague.sis to te.t if the.pe.ce.tion of pre.ation risk from alarm cue.（conspe.ific skin e.tract） alone.induce. an incre.se. re.ative.body de.th which is a de.e.se.against gape.limite. pre.ators. Afte. two we.ks of e.posure. siblings that we.e.e.pose. to conspe.ific alarm cue.incre.se. the.r re.ative.body de.th ne.rly double.the.amount of those.e.pose. to distille. wate. （control） and ze.rafish Danio re.io alarm cue. We.re.e.te. our me.sure.e.ts ove. the.last two we.ks （12 and 14） of cue.e.posure.whe. the.fish we.e.late.stage.juve.ile. to te.t if the.rate.of incre.se.was sustaine.; the.e.we.e.no diffe.e.ce. in final dime.sions be.we.n the.thre. tre.tme.ts. Our re.ults show that 1） the.Nicaragua cichlid has an innate.re.ponse.to conspe.ific alarm cue.which is not a ge.e.alize. re.ponse.to an injure. fish, and 2） this innate.re.ognition ultimate.y re.ults in de.e.oping a de.pe. body at a stage.of the.life.history whe.e.pre.ation risk is high [Curre.t Zoology 56 （1）： 36-42, 2010].

关键词： Alarm substance Inducible.de.e.se Phe.otypic plasticity Cichlid Che.ical cue Antipre.ator

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Macro and micro issue. in turbule.t mixing

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Scie.ce.China(Te.hnological Scie.ce.) 2013年第10期56卷 2355-2360页

作者： Me.VIN J kaufman R LIM H KAMAN T RAO P GLIMM J De.artme.t of Applie. Mathe.atics and Statistics Stony Brook UniversityStony Brook NY 11794 USA

Nume.ical pre.iction of turbule.t mixing can be.divide. into two subproble.s: to pre.ict the.ge.me.rical e.te.t of a mixing re.ion and to pre.ict the.mixing prope.tie. on an atomic or mole.ular scale. within the.mixing re.ion. The.forme. goal suffice. for some.purpose., while.important proble.s of che.ical re.ctions(e.g. flame.) and nucle.r re.ctions de.e.d critically on the.se.ond goal in addition to the.first one. He.e.we.re.ie. re.e.t progre.s in e.tablishing a conce.tual re.ormulation of conve.ge.ce. and we.illustrate.the.e.conce.ts with a re.ie. of re.e.t nume.ical studie. addre.sing turbule.ce.and mixing in the.high Re.nolds numbe. limit. We.re.ie. significant progre.s on the.first goal, re.arding the.mixing re.ion, and initial progre.s on the.se.ond goal, re.arding atomic le.e. mixing prope.tie.. Ne. re.ults conce.ning non-unique.e.s of the.infinite.Re.nolds numbe. solutions and othe. conse.ue.ce. of a re.ormalization group point of vie., to be.publishe. in de.ail e.se.he.e. are.summarize. *** notion of stochastic conve.ge.ce.of probability me.sure. and probability distribution functions) re.lace. traditional pointwise.conve.ge.ce. The.primary be.e.it of this ide. is its incre.se. stability re.ative.to the.statistical "noise. which characte.ize. turbule.t flow. Our re.ults also show that this modification of conve.ge.ce. with sufficie.t me.h re.ine.e.t, may not be.ne.de.. Howe.e., in practice. me.h re.ine.e.t is se.dom sufficie.t and the.stochastic conve.ge.ce.conce.ts have.a *** to this circle.of ide.s is the.obse.vation that turbule.t mixing, in the.limit of high Re.nolds numbe., appe.rs to be.non-unique. Not only have.multiple.solutions be.n obse.ve.(and publishe.) for ide.tical proble.s, but simple.physics base. argume.ts and more.re.ine. argume.ts base. on the.re.ormalization group come.to the.same.*** of the.non-unique.e.s inhe.e.t in nume.ical mode.s of high Re.nolds numbe. turbule.ce.and mixing, we.also include.he.e.nume.i

关键词： stochastic conve.ge.ce turbule.t mixing re.ormalization group dynamic subgrid scale.mode.s

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INVe.TIGATION OF He.Pe. SIMPLe. VIRUS TYPe.1 LATe.CY IN CORNe.S

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中华医学杂志(英文版) 1993年第4期106卷 288-291页

作者：谢立信董晓光 He.be.t e.kaufman Institute.of Ophthalmology Shandong Academy of Medical SciencesQingdao 266033 LSU e.e.Ce.te. New OrleansLAUSA

The.re.urre.t rate.of he.pe. simple. ke.atitis (HSK) was 4.17% afte. pe.e.rating ke.atoplasty (PKP) was pe.forme. in 216 patie.ts with HSK; the.me.n pe.iod of follow-up was 6.5 ye.rs, Immunohistoche.ical te.hnique.showe. that HSV-1 antige.s we.e.pre.e.t in the.corne.l stroma of rabbit e.e. up to 45 days afte. primary corne.l infe.tion had subside.. No positive.staining of HSV antige. was found in the.se.tions of 18 human corne.l buttons with quie.ce.t he.pe.ic le.ions. Howe.e., afte. coculture. with primary rabbit kidne. (PRK) ce.ls, positive.staining was note. in 6/9 human corne.l buttons, and some.PRK ce.ls de.e.ope. cytopathic e.fe.t. The.de.e.tion of the.se.sitivity of culture. corne.l ce.ls to HSV-1 infe.tion de.onstrate. that ke.atocyte. we.e.the.most se.sitive.ce.ls. The.e.re.ults sugge.t that the.corne. may be.anothe. site.of HSV-1 late.cy in addition to the.trige.inal gangalion.

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Re.ie. of radiographic findings in COVID-19

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World Journal of Radiology 2020年第8期12卷 142-155页

作者： Audre. e.kaufman Sonum Naidu Sarayu Ramachandran Dalia S kaufman Zahi A Fayad Ve.kate.h Mani De.artme.t of Radiology BioMedical Engineering and Imaging InstituteIcahn School of Medicine at Mount SinaiHess Center for Science and MedicineNew YorkNY 10029United States

The.purpose.of this study is to re.ie. the.publishe. lite.ature.for the.range.ofradiographic findings pre.e.t in patie.ts suffe.ing from coronavirus dise.se.2019infe.tion. This nove. corona virus is curre.tly the.cause.of a worldwide.*** symptoms and signs dominate.the.clinical picture.and radiologists are.alle. upon to e.aluate.che.t radiographs (CXR) and compute. tomography (CT)image. to asse.s for infiltrate. and to de.ine.the.r e.te.t, distribution andprogre.sion. Multiple.studie. atte.pt to characte.ize.the.dise.se.course.bylooking at the.timing of imaging re.ative.to the.onse. of symptoms. In ge.e.al,plain CXR show bilate.al dise.se.with a te.de.cy toward the.lung pe.iphe.y andhave.an appe.rance.most consiste.t with viral pne.monia. Che.t CT image. are.ost notable.for showing bilate.al and pe.iphe.al ground glass and consolidate.opacitie. and are.marke. by an abse.ce.of concomitant pulmonary nodule.,cavitation, ade.opathy and ple.ral e.fusions. Publishe. lite.ature.me.tioningorgan syste.s aside.from pulmonary manife.tations are.re.ative.y le.s common,ye. pre.e.t and are.addre.se. in this re.ie.. Similarly, publications focusing onimaging modalitie. aside.from CXR and che.t CT are.sparse.in this e.olving crisisand are.like.ise.addre.se. in this re.ie.. The.role.of imaging is e.amine. as it iscurre.tly be.ng de.ate. in the.me.ical community, which is not at all surprisingconside.ing the.highly infe.tious nature.of Se.e.e.Acute.Re.piratory Syndrome.oronavirus 2.

关键词： Coronavirus COVID-19 Pne.monia Compute. tomography scan X-Ray Pande.ics

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Fe.sibility of imaging supe.ficial palmar arch using microultrasound,7T and 3T magne.ic re.onance.imaging

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World Journal of Radiology 2017年第2期9卷 79-84页

作者： Alison N Pruzan Audre. e.kaufman Claudia Calcagno Yu Zhou Zahi A Fayad Ve.kate.h Mani Translational and Mole.ular Imaging Institute Department of RadiologyIcahn School of Medicine at Mount SinaiNew YorkNY 10029United States

AIM To de.onstrate.fe.sibility of ve.se. wall imaging of the.supe.ficial palmar arch using high fre.ue.cy microultrasound, 7T and 3T magne.ic re.onance.imaging(MRI).Me.HODS Four subje.ts(age. 22-50 ye.rs) we.e.scanne. on a micro-ultrasound syste. with a 45-MHz transduce.(Ve.o 2100, Visual Sonics). Subje.ts' hands we.e.the. image. on a 3T clinical MR scanne.(Sie.e.s Biograph MMR) using an 8-channe. spe.ial purpose.phase. array carotid coil. Lastly, subje.ts' hands we.e.image. on a 7T clinical MR scanne.(Sie.e.s Magne.om 7T Whole.Body Scanne.) using a custom built 8-channe. transmit re.e.ve.carotid coil. All thre. imaging modalitie. we.e.subje.tive.y analyze. for image.quality and visualization of the.ve.se. wall. Re.ULTS Re.ults of this ve.y pre.iminary study indicate. that ve.se. wall imaging of the.supe.ficial palmar arch was fe.sible.with a whole.body 7T and 3T MRI in comparison with micro-ultrasound. Subje.tive.analysis of image.quality(1-5 scale. 1: poore.t, 5: be.t) from B mode. ultrasound, 3T SPACe.MRI and 7T SPACe.MRI indicate. that the.image.quality obtaine. at 7T was supe.ior to both 3T MRI and micro-ultrasound. The.3D SPACe.se.ue.ce.at both 7T and 3T MRI with isotropic voxe.s allowe. for multi-planarre.ormatting of image. and allowe. for le.s ope.ator de.e.de.t re.ults as compare. to high fre.ue.cy microultrasound imaging. Although quantitative.analysis re.e.le. that the.e.was no significant diffe.e.ce.be.we.n the.thre. me.hods, the.7T Te.la tre.de. to have.be.te. visibility of the.ve.se. and its wall. CONCLUSION Imaging of smalle. arte.ie. at the.7T is fe.sible.for e.aluating athe.oscle.osis burde. and may be.of clinical re.e.ance.in multiple.dise.se..

关键词： Supe.ficial Palmar Arch 7T and 3T magne.ic re.onance.imaging Micro-ultrasound Athe.oscle.osis Cardiovascular dise.se

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Histologically prove. he.atic ste.tosis associate. with lowe. te.toste.one.le.e.s in me. with obe.ity

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Asian Journal of Andrology 2020年第3期22卷 252-257页

作者： Fre.e.ique.Van de.Ve.de.Marlie. Be.ae.t Anne.Hoore.s Anja Ge.rts Guy T'Sjoe. Tom Fie.s Je.n-Marc kaufman Yve. Van Nie.we.hove.Bruno Lapauw de.artme.t of e.docrinology Ghent University Hospital9000 GhentBelgium de.artme.t of Pathology Ghent University Hospital9000 GhentBelgium De.artme.t of He.athology Ghent University Hospital9000 GhentBelgium De.artme.t of Clinical Biology Ghent University Hospital9000 GhentBelgium De.artme.t of Gastro-Inte.tinal Surge.y Ghent University Hospital9000 GhentBelgium.

Me. with obe.ity ofte. pre.e.t with low te.toste.one.T)and se. hormone.binding globulin(SHBG)*** me.hanisms for this have.be.n propose.,but as SHBG is se.re.e. by he.atocyte. and se. ste.oids unde.go he.atic me.abolization,this study inve.tigate. whe.he. se.e.ity and histological compone.ts of nonalcoholic fatty live. dise.se.NAFLD)are.associate. with se. ste.oid le.e.s in obe.e.*** cross-se.tional study include. 80 obe.e.me.(age.46±11 ye.rs;body mass inde.:42.2±5.5 kg m^-2).Se.um le.e.s of total T and e.tradiol(e.)we.e.me.sure. using liquid chromatography couple. with tande. mass spe.troscopy(LC/MS-MS)and SHBG and gonadotropins by *** biopsie. we.e.e.aluate. using ***,and Fibrosis *** with ste.tohe.atitis had similar me.ian(1st quartile.3rd quartile.total T le.e.s(7.6[5.0-11.0]nmol l^-1 vs 8.2[7.2-10.9]nmol l^-1;P=0.147),lowe. calculate. fre. T(cFT)le.e.s(148.9[122.9-188.8]pmol 1-1 s 199.5[157.3-237.6] pmol l^-1;P=0.006),and highe. fre. e.T ratios(10.0[6.4-13.9]×10^-3 vs 7.1[5.7-10.7]×10^-3;P=0.026)compare. to me. with only nonalcoholic fatty *** the.histological compone.ts of *** ste.tosis was inde.e.de.tly associate. with total T(r=-0.331.P=0.003)and cFT le.e.s(r=-0.255.P=0.025).Obe.e.me. *** have.e.e. lowe. cFT le.e.s compare. to those.without,an association mainly drive. by grade.of *** this re.le.ts a subgroup of me. with a more.se.e.e.obe.ity-re.ate. phe.otype.or re.ults from dire.t re.ations be.we.n he.atic ste.tosis and se. ste.oid me.abolism ne.ds furthe. inve.tigation.

关键词： male.hypog on adism non alcoholic fatty live. dise.se obe.ity se. ste.oids ste.tosis te.toste. one

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e.fe.t of varying compute. tomography acquisition and re.onstruction parame.e.s on se.i-automate. clot volume.quantification

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World Journal of Radiology 2018年第3期10卷 24-29页

作者： Audre. e.kaufman Alison N Pruzan Ching Hsu Sarayu Ramachandran Adam Jacobi Zahi A Fayad Ve.kate.h Mani De.artme.t of Radiology Icahn School of Medicine at Mount SinaiNew YorkNY 10029United States Translational and Mole.ular Imaging Institute Icahn School of Medicine at Mount SinaiHess Center for Science and MedicineNew YorkNY 10029United States Daiichi Sankyo Inc. Basking RidgeNJ 07920United States

AIM To e.amine.e.fe.ts of compute. tomography(CT)image.acquisition/re.onstruction parame.e.s on clot volume.quantification in vitro for re.e.rch me.hod validation *** This study was pe.forme. in conformance.with HIPAA and IRB Re.ulations(March 2015-Nove.be. 2016).A te. blood clot phantom was de.igne. and scanne. on a dual-e.e.gy CT scanne.(SOMATOM Force.Sie.e.s He.lthcare.Gm BH,e.lange.,Ge.many)with varying pitch,ite.ative.re.onstruction,e.e.gy le.e. and slice.hickne.s.A range.of clot and tube.size. we.e.use. in an atte.pt to re.licate.in vivo e.boli found within ce.tral and se.me.tal branche. of the.pulmonary arte.ie. in patie.ts with pulmonary *** volume.was the.me.sure. parame.e. and was analyze. by a single.image.analyst using a se.i-automate. re.ion growing algorithm imple.e.te. in the.FDA-approve. Sie.e.s *** image.analysis *** mode. analysis was pe.forme. on the.*** On the.acquisition side.the.continuous factor of e.e.gy showe. no statistically significant e.fe.t on absolute.clot volume.quantification(P=0.9898).On the.othe. hand,whe. conside.ing the.fixe. factor of pitch,the.e.we.e.statistically significant diffe.e.ce. in clot volume.quantification(Pe.re.onstruction side.with the.continuous factor of re.onstruction slice.thickne.s no statistically significant e.fe.t on absolute.clot volume.quantification was de.onstrate.(P=0.4500).Also on the.re.onstruction side.with the.fixe. factor of using ite.ative.re.onstructions the.e.was also no statistically significant e.fe.t on absolute.clot volume.quantification(P=0.3011).In addition,the.e.was e.ce.le.t R^2 corre.ation be.we.n the.scale.me.sure. mass of the.clots both with re.pe.t to the.CT me.sure. volume. and with re.pe.t to volume. me.sure.by the.wate. displace.e.t *** Aside.from varying pitch,changing CT acquisition parame.e.s and using ite.ative.re.onstructions had no significant impact on clot volume.quantification with a se.i-automate

关键词： Compute. tomography angiography Radiographic phantom Compute.-assiste. image analysis Pulmonary e.bolism Thrombolytic the.apy

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家禽体外寄生虫的控制

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中国家禽 2008年第12期30卷 12-16页

作者： P.e.kaufman P.G.Koe.le. J.F.Butle. 佛罗里达州立大学

虱子、恙虫、家禽壁虱和鸡蚤是家禽业中常见的体外寄生虫。虽然禽舍苍蝇不是寄生虫,但是备受家禽生产者的关注,尤其对于笼养蛋鸡生产者。这些体外寄生虫对家禽业的危害日益得到重视。本专题就上述几种体外寄生虫的生活习性和药物控制措... 详细信息

虱子、恙虫、家禽壁虱和鸡蚤是家禽业中常见的体外寄生虫。虽然禽舍苍蝇不是寄生虫,但是备受家禽生产者的关注,尤其对于笼养蛋鸡生产者。这些体外寄生虫对家禽业的危害日益得到重视。本专题就上述几种体外寄生虫的生活习性和药物控制措施进行总结,希望为广大读者提供借鉴。

关键词：体外寄生虫药物控制家禽业笼养蛋鸡生活习性生产者虱子苍蝇

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