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Withania somnifera (Ashwagandha):a Novel Source of L-asparaginase

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Journal of Integrative Plant Biology 2009年第2期51卷 201-206页

作者： Vishal P.Oza Shraddha d.Trivedi Pritesh P.parmar R.B.Subramanian B.R.d.School of Biosciences Sardar Patel UniversityV.V.Nagar 388 120 (Gujarat)India B.R.d.School of Biosciences Sardar Patel UniversityV.V.Nagar 388120 (Gujarat)India

different parts of plant species belonging to Solanaceae and Fabaceae families were screened for L-asparaginase enzyme （E.C.3.***.1.1.）. Among 34 plant species screened for L-asparaginase enzyme. Withania somnifera L. was identified as a potential source of the enzyme on the basis of high specific activity of the enzyme. The enzyme was purified and characterized from W. somnifera, a popular medicinal plant in South East Asia and Southern Europe. Purification was carried out by a combination of protein precipitation with ammonium sulfate as well as Sephadex-gel filtration. The purified enzyme is a homodimer, with a molecular mass of 72 ± 0.5 kda as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresisand size exclusion chromatography. The enzyme has a pH optimum of 8.5 and an optimum temperature of 37 ℃. The Km value for the enzyme is 6.1 × 10^-2 mmol/L. This is the first report for L-asparaginase from W. somnifera, a traditionally used Indian medicinal plant.

关键词： Fabaceae L-asparaginase purification Sotanaceae Withania somnifera.

Quantitation of tadalafil in human plasma using a sensitive and rapid LC-MS/MS method for a bioequivalence study

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Journal of Pharmaceutical Analysis 2018年第4期8卷 271-276页

作者： Abhaysingh Bhadoriya Bhavesh dasandi dharmesh parmar Priyanka A. Shah Pranav S. Shrivastav Bioanalytical department Synchron Research Services Pvt. Ltd. department of Chemistry School of Sciences Gujarat University

A highly sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method was developed for the determination of tadalafil（TAd） in human plasma. TAd and its deuterated internal standard（IS）, tadalafil-d3, were extracted from 200 mL plasma using Phenomenex Strata-X-C 33 m extraction cartridges. Chromatographic analysis was carried out on Synergi Hydro-RP C18（100 mm × 4.6 mm, 4 mm）column with a mobile phase consisting of methanol and 10 m M ammonium formate, p H 4.0（90：10, v/v）,delivered at a flow rate of 0.9 m L/min. Quantitation of the protonated analyte was done on a triple quadrupole mass spectrometer using multiple reaction monitoring via electrospray ionization. The precursor to product ions transitions monitored for TAd and TAd-d3 were m/z 390.3-268.2 and m/z393.1-271.2, respectively. The calibration curve was linear over the concentration range of 0.50–500 ng/m L with correlation coefficient, r2 Z 0.9994. Acceptable intra-batch and inter-batch precision（r3.7%） and accuracy（97.8% to 104.1%） were obtained at five concentration levels. The recovery of TAd from spiked plasma was highly precise and quantitative（98.95% to 100.61%）. Further, the effect of endogenous matrix components was minimal. TAd was found to be stable under different storage conditions in human plasma and also in whole blood samples. The validated method was successfully used to determine TAd plasma concentration in a bioequivalence study with 20 mg TAd tablets in 24 healthy volunteers. Method performance was evaluated by reanalyzing 115 study samples.

关键词： Tadalafil Tadalafil-d3 LC-MS/MS Sensitive Bioequivalence study Human plasma

Sensitive and rapid determination of amantadine without derivatization in human plasma by LC–MS/MS for a bioequivalence study

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Journal of Pharmaceutical Analysis 2018年第3期8卷 202-207页

作者： Abhaysingh Bhadoriya Shivprakash Rathnam Bhavesh dasANdi dharmesh parmar Mallika Sanyal Pranav S.Shrivastav Bioanalytical department Synchron Research Services Pvt.Ltd department of Chemistry St.Xavier's College department of Chemistry School of Sciences Gujarat University

A highly sensitive, rapid and rugged liquid chromatography-tandem mass spectrometw （LC-ESI-MS/MS） method was developed for reliable estimation of amantadine （AMd）, an antiviral drug in human plasma. The analyte and internal standard （IS）, amantadine-d6 （AMd-d6）, were extracted from 200μL plasma by solid phase extraction on Phenomenex Strata-X-C 33 ~t cartridges. Chromatography was performed on Synergi^TM Hydro-RP C18 （150 mm×4.6 mm, 4 μm） analytical column using a mixture of acetonitrile and 10 mM ammonium formate, pH 3.0 （80：20, v/v） as the mobile phase. detection and quantitation was done by multiple reaction monitoring in the positive ionization mode for AMd （m/z 152.1→ 135.1 ） and IS （m/z 158.0 → 141.1） on a triple quadrupole mass spectrometer. The assay was linear in the concentration range of 0.50-500 ng/mL with correlation coefficient （r^2）〉 0.9969. The limit of detection of the method was 0.18 ng/mL The intra-batch and inter-batch precisions were 〈 5.42% and the accuracy varied from 98.47% to 105.72%. The extraction recovery of amantadine was precise and quantitative in the range of 97.89%-100.28%. IS-normalized matrix factors for amantadine varied from 0.981 to 1.012. The stability of AMd in whole blood and plasma was evaluated under different conditions. The developed method was successfully applied for a bioequivalence study with 100 mg of AMd in 32 healthy volunteers. The reproducibility of the assay was determined by reanalysis of 134 subject samples.

关键词： Amantadine Amantadine-d6 LC-ESI-MS/MS Sensitive Bioequivalence study Human plasma

Identification of Potential Species Croton bonplandianum, Sedges and Balanitis aegyptiacafor the Application of Phytoremediation

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American Journal of Plant Sciences 2013年第6期4卷 1246-1251页

作者： Pritesh parmar Bhaumik dave Ketankumar Panchal R. B. Subramanian B R d School of Biosciences Sardar Patel University Vallabh Vidya India.

Heavy metal contamination to the environment is a serious problem in the developing countries due to anthropogenic activities, a threat needs to remediate to sustain the life on earth, technology includes use of microorganisms and different plant species. In consideration of biomass, phytoremediation is a very useful techniques above all, can be exploit by identification of hyperaccumulator, which accumulates the heavy metal under metal stress condition. In view of constraints of efficient plant species in present study thirty seven different plant species were screened for the identification of heavy metal accumulators. Croton bonplandianum, sedges and Balanites aegyptiaca amongst the all exhibit superior potential of heavy metal accumulation. This is the first report to unravel the heavy metal accumulation property of three different plant species which can be exploited for the bioremediation of heavy metals.

关键词： Balanitis aegyptiaca Croton bonplandianum Hyperaccumulator Phytoremediation Screening Sedges

采用CTPA人工智能驱动算法自动检测肺栓塞

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国际医学放射学杂志 2021年第1期44卷 120-121页

作者： T.Weikert d.J.Winkel J.Bremerich B.Stieltjes V.parmar A.W.Sauter 张晓东

摘要目的基于大数据测试CT肺血管成像(CTPA)人工智能驱动算法自动检测肺栓塞(PE)的表现。材料与方法回顾性分析2017年本中心全部CTPA数据(n=1 499)。临床

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dynamism of Antimicrobial Activity of Actinomycetes—A Case Study from Undisturbed Microbial Niche

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Advances in Microbiology 2014年第6期4卷 324-334页

作者： Jayshree d. Patel Mahesh parmar Purshottam Patel Pravin Rohit Ramesh Taviyad Parveen Ansari Bipasha Bhattacharya dhaval Vyas Vipin Kumar Nirmal S. Sahay Pawan K. Singh National Innovation Foundation India Satellite Complex Nr. Mansi Circle Ahmedabad Gujarat India Sadbhav SRISTI Sanshodhan Laboratory AES Boys Hostel Campus Nr. Gujarat University Library & SBI Bank Navrangpura Ahmedabad Gujarat India

Escalation of drug resistant microbes (bacteria) had forced researchers to search new and improved therapeutic compounds from different possible sources, including metabolites secreted by the actinomycetes. The aim of this study was to evaluate the pattern of antimicrobial actinomycetes from physiologically distinct soil of different geographical locations. Forty five soil samples were collected from 5 districts of Gujarat including two sanctuaries as source of survey for bioactive actinomycetes. Crowded plate technique was used for isolation and Agar cylinder method was employed for the antimicrobial screening. A total of 171 actinomycetes were isolated and screened against eighteen pathogens responsible for causing diseases in plants and humans. Results indicate that 79% of the isolates were active against at least one of the eighteen tested pathogens. Some of the actinomycetes strain had shown strong antibacterial and antifungal activity which may be a good source of obtaining novel antimicrobials.

关键词： Actinomycetes Screening Antimicrobial drug Resistance Soil Microflora

Extensive study of optical contrast between bulk and nanoscale transition metal dichalcogenide semiconductors

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Journal of Semiconductors 2021年第8期42卷 45-54页

作者： Ankush parmar Jashangeet Kaur Manish dev Sharma Navdeep Goyal Centre of Applied Sciences Department of PhysicsSector-14Panjab UniversityChandigarh 160014India department of Physics G.G.D.S.D CollegeSector-32Chandigarh 160030India

A remarkable refinement in the optical behavior of two-dimensional transition metal dichalcogenides(TMds)has been brought to light when cleaved from their respective *** atomically thin direct bandgap semiconductors are highly responsive to optical energy which proposes the route for futuristic photonic *** this manuscript,we have substantially focused on the optical study of MoS_(2)and WS_(2)nanosheets and comparative analysis with their bulk *** synthesis of nanosheets has been accomplished with liquid exfoliation followed by fabrication of thin films with drop-casting technique.X-ray diffraction and field emission scanning electron microscopy affirmed the morphology,whereas,UV-visible spectroscopy served as the primary tool for optical *** was observed that several parameters,like optical conductivity,optical band-gap energy *** enhanced statistics in the case of exfoliated nanosheets as compared to their respective *** researchers have touched upon this analysis for MoS_(2),but it is completely novel for WS_(2).We expect our work to clearly distinguish between the optical behaviors of nanoscale and bulk TMds so as to intensify and strengthen the research related to 2d-layered materials for optoelectronic and photovoltaic applications.

关键词： transition metal dichalcogenides 2d layered materials optical properties liquid phase exfoliation thin films dropcasting

Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum

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American Journal of Molecular Biology 2013年第4期3卷 241-247页

作者： Pritesh parmar Ankit Sudhir R. Preethi Bhaumik dave Ketankumar Panchal Ramalingam B. Subramanian Arvind Patel K. B. Kathiria B. R. d. School of Biosciences Sardar Patel University Vallabh Vidyanagar India Vegetable research station Anand Agricultural University Anand India

With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and to further facilitate the use of markers in tomato breeding. The present study is a step to introduce a new SSR marker (TOM-144) which was deduced after evaluation of eight microsatellite loci amongst the twenty-one different tomato cultivars. The marker selected was inherited and segregated in mendelian fashion as demonstrated in successive generation of a cross between parent cvs. H-24 x GT-2.

关键词： Fusarium Wilt Molecular Marker Tomato

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