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Clinical Value of Hepatoma-Specific Alpha-Fetoprotein in the Diagnosis of Hepatocellular Carcinoma

Chinese Journal of Clinical Oncology 2006年第3期3卷 153-157页

作者： runzhou ni mingbing xiao fei jin cuihua lu jiefei huang xianyong meng Department of Gastroenterology Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China.

OBJECTIVE To study the clinical value of hepatoma-specific alpha-feto- protein (HS-AFP) in the diagnosis and differential diagnosis of hepato- cellular carcinoma (HCC). METHODS A method of vertical slab polyacrylamide gel electrophoresis with discontinuous buffer system was developed to separate AFP sub- types. After separation, the AFP subtypes were transferred to nitrocellu- lose and reacted first with rabbit anti-human AFP and then with goat an- ti-rabbit IgG -HRP. Finally, AFP subtypes were visualized by reacting with 3,3’-diaminobenzidine tetrahydrochloride. A HS-AFP band was de- termined in 82 cases with HCC and 95 cases with benign liver diseases. The correlations between the positive rates of HS-AFP and serum AFP concentration, tumor size as well as portal vein metastasis were analyzed. RESULTS Serum AFP in the cases with various liver diseases was sepa- rated into one to several bands. The fastest band on electrophoresis (F- AFP) was found in all patients, while the band at the cathodal site (HS- AFP) was detected predominantly in HCC but rarely in benign liver dis- eases. The positive rate of HS-AFP in HCC was 74.4%, which was sig- nificantly higher than that in benign liver diseases (9.1%, 7.3% and 10.0% in liver cirrhosis, chronic hepatitis and acute hepatitis respectively). HS- AFP was detected in 3 out of 9 HCC cases with AFPnign liver diseases with the same AFP concentra- tion. HS-AFP correlated with serum AFP concentration and tumor size to some extent, but not with portal vein metastasis. CONCluSION HS-AFP increases the sensitivity of diagnosing HCC in patients with negative AFP, and is useful in distinguishing high AFP due to HCC from that caused by benign liver diseases.

关键词： hepatoma alpha-fetoprotein heterogeneity diagnosis.

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