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Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit

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Journal of Pharmaceutical Analysis 2023年第1期13卷 55-62页

作者： Runshan Will Jiang Karol Jaroch Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooN2L 3G1Canada Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in TorunBydgoszcz85-089Poland

Improved analytical methods for the metabolomic profiling of tissue samples are constantly needed.Currently,conventional sample preparation methods often involve tissue biopsy and/or homogenization,which disrupts the endogenous metabolome.In this study,solid-phase microextraction(SPME)fibers were used to monitor changes in endogenous compounds in homogenized and intact ovine lung tissue.Following SPME,a Biocrates AbsoluteIDQ assay was applied to make a downstream targeted metabolomics analysis and confirm the advantages of in vivo SPME metabolomics.The AbsoluteIDQ kit enabled the targeted analysis of over 100 metabolites via solid-liquid extraction and SPME.Statistical analysis revealed significant differences between conventional liquid extractions from homogenized tissue and SPME results for both homogenized and intact tissue samples.In addition,principal component analysis revealed separated clustering among all the three sample groups,indicating changes in the metabolome due to tissue homogenization and the chosen sample preparation method.Furthermore,clear differences in free metabolites were observed when extractions were performed on the intact and homogenized tissue using identical SPME procedures.Specifically,a direct comparison showed that 47 statistically distinct metabolites were detected between the homogenized and intact lung tissue samples(P<0.05)using mixed-mode SPME fibers.These changes were probably due to the disruptive homogenization of the tissue.This study's findings highlight both the importance of sample preparation in tissue-based metabolomics studies and SPME's unique ability to perform minimally invasive extractions without tissue biopsy or homogenization while providing broad metabolite coverage.

关键词： Solid-phase microextraction Solvent extraction Metabolomics Sample preparation In vivo sampling

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Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma

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Journal of Pharmaceutical Analysis 2023年第2期13卷 216-222页

作者： Wei Zhou Martyna N.Wieczorek Runshan Will Jiang Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooONN2L 3G1Canada Faculty of Food Science and Nutrition Poznan University of Life SciencesPoznanPoland

The direct coupling of solid-phase microextraction(SPME)to mass spectrometry(MS)(SPME-MS)has proven to be an effective method for the fast screening and quantitative analysis of compounds in complex matrices such as blood and plasma.In recent years,our lab has developed three novel SPME-MS techniques:SPME-microfluidic open interface-MS(SPME-MOI-MS),coated blade spray-MS(CBS-MS),and SPME-probe electrospray ionization-MS(SPME-PESI-MS).The fast and high-throughput nature of these SPME-MS technologies makes them attractive options for point-of-care analysis and anti-doping testing.However,all these three techniques utilize different SPME geometries and were tested with different MS instruments.Lack of comparative data makes it difficult to determine which of these methodologies is the best option for any given application.This work fills this gap by making a comprehensive comparison of these three technologies with different SPME devices including SPME fibers,CBS blades,and SPME-PESI probes and SPME-liquid chromatography-MS(SPME-LC-MS)for the analysis of drugs of abuse using the same MS instrument.Furthermore,for the first time,we developed different desorption chambers for MOI-MS for coupling with SPME fibers,CBS blades,and SPME-PESI probes,thus illustrating the universality of this approach.In total,eight analytical methods were developed,with the experimental data showing that all the SPME-based methods provided good analytical performance with R^(2)of linearities larger than 0.9925,accuracies between 81%and 118%,and good precision with an RSD%≤13%.

关键词： Solid-phase microextraction Mass spectrometry Microfluidic open interface Coated blade spray Probe electrospray ionization Drug of abuse

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磷尾矿粉对混凝土力学性能的影响

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新乡学院学报 2024年第3期41卷 44-46页

作者：卜娜蕊江伟吕浩璋白润山河北建筑工程学院土木工程学院河北张家口075000 河北省土木工程诊断改造与抗灾重点实验室河北张家口075000

为了研究磷尾矿粉作为矿物掺合料对混凝土力学性能的影响,采用不同掺量的磷尾矿粉加入到混凝土中,研究不同掺量的磷尾矿粉对混凝土力学性能的影响。研究结果表明,适量磷尾矿粉的掺入可以在一定程度上改善混凝土的性能:当掺入6%的磷尾矿... 详细信息

为了研究磷尾矿粉作为矿物掺合料对混凝土力学性能的影响,采用不同掺量的磷尾矿粉加入到混凝土中,研究不同掺量的磷尾矿粉对混凝土力学性能的影响。研究结果表明,适量磷尾矿粉的掺入可以在一定程度上改善混凝土的性能:当掺入6%的磷尾矿粉时7 d龄期混凝土的强度提高是最大的,7 d龄期混凝土的强度相对于基准期强度提高了12.2%;当掺入12%的磷尾矿粉时28 d龄期混凝土的强度提高是最大的,28 d龄期混凝土的强度相对于基准期强度提高了11.0%。

关键词：磷尾矿粉混凝土力学性能

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