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Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip

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Journal of Pharmaceutical Analysis 2022年第5期12卷 808-813页

作者： Jun Xu Qing Tang runhui zhang Haoyi Chen Bee Luan Khoo Xinguo zhang Yue Chen Hong Yan Jincheng Li Huaze Shao Lihong Liu NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou510515China The Second Clinical Medical School Southern Medical UniversityGuangzhou510515China Department of Biomedical Engineering City University of Hong KongHong Kong999077China

The identification of tumor-related microRNAs(miRNAs)exhibits excellent promise for the early diagnosis of cancer and other bioanalytical ***,we developed a sensitive and efficient biosensor using polyadenine(polyA)-mediated fluorescent spherical nucleic acid(FSNA)for miRNA analysis based on strand displacement reactions on gold nanoparticle(AuNP)surfaces and electrokinetic signal amplification(ESA)on a microfluidic *** this FSNA,polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and *** upright conformational polyA-DNA recognition block hybridized with 6-carboxyfluorescein-labeled reporter-DNA,resulting in fluorescence quenching of FSNA probes induced by AuNP-based resonance energy *** DNA was replaced in the presence of target miRNA,leading to the recovery of reporter-DNA ***,reporter-DNAs were accumulated and detected in the front of with Nafion membrane in the microchannel by *** method showed high selectivity and sensitivity with a limit of detection of 1.3 *** method could also be used to detect miRNA-21 in human serum and urine samples,with recoveries of 104.0%-113.3% and 104.9%-108.0%,***,we constructed a chip with three parallel channels for the simultaneous detection of multiple tumor-related miRNAs(miRNA-21,miRNA-141,and miRNA-375),which increased the detection *** universal method can be applied to other DNA/RNA analyses by altering recognition sequences.

关键词： MicroRNAs Microfluidic chip Electrokinetic signal amplification Polyadenine-DNA Gold nanoparticle

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倾斜管道内圆柱状颗粒运动的数值模拟

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力学与实践 2022年第5期44卷 1172-1178页

作者：李亮刘炳瑞张润辉张斌浙江大学力学系流体工程研究所杭州310027 广东盈峰智能环卫科技有限公司广东佛山528322 南华大学湖南衡阳421009

本文采用计算流体力学-离散单元法对倾斜管道内圆柱颗粒在气体中的运动进行数值模拟,分别分析了颗粒密度、颗粒长径比、吸入速度、管道倾角、管道长径比等因素对颗粒输运效率的影响。结果表明,颗粒密度越大,颗粒的输运效率越低;输运效... 详细信息

本文采用计算流体力学-离散单元法对倾斜管道内圆柱颗粒在气体中的运动进行数值模拟,分别分析了颗粒密度、颗粒长径比、吸入速度、管道倾角、管道长径比等因素对颗粒输运效率的影响。结果表明,颗粒密度越大,颗粒的输运效率越低;输运效率随着颗粒长径比的增大而减小;输运效率随着进口速度的增大而增大。管道的倾角对输运效率存在影响,但二者不是单调关系;颗粒输运效率与管道长径比成反比。

关键词：输运效率倾斜管道柱状颗粒离散单元法

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扫路机气力系统工作效率研究

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力学与实践 2022年第1期44卷 45-50页

作者：李亮刘炳瑞张润辉浙江大学航空航天学院流体所流体动力与机电系统国家重点实验室杭州310027 广东盈峰智能环卫科技有限公司广东佛山528322

本文旨在探讨提高吸式扫路机气力输送系统的效率,采用计算流体力学与离散单元法对圆柱颗粒在气体中的运动进行数值模拟,分别研究了颗粒密度、颗粒长径比、颗粒初始平面与吸入口的距离、吸入速度、颗粒所处圆周对于颗粒吸入效率的影响。... 详细信息

本文旨在探讨提高吸式扫路机气力输送系统的效率,采用计算流体力学与离散单元法对圆柱颗粒在气体中的运动进行数值模拟,分别研究了颗粒密度、颗粒长径比、颗粒初始平面与吸入口的距离、吸入速度、颗粒所处圆周对于颗粒吸入效率的影响。研究结果表明:颗粒密度越大,颗粒的吸入效率越低;颗粒长径比对吸入效率的影响较小;颗粒初始平面与吸入口的距离与颗粒吸入效率为负相关;吸入速度增大有利于提高颗粒吸入效率,但是当吸入速度增大至一定程度时,其对吸入效率影响减小;颗粒所处圆周与吸入口圆周径向距离越小,颗粒的吸入效率越高。

关键词：气力系统输运效率柱状颗粒离散单元法

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生物质热化学转化的CFD模拟研究

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力学与实践 2021年第5期43卷 663-673页

作者：库晓珂王金陈涛张润辉浙江大学航空航天学院工程力学系

从颗粒多相流计算流体力学(computational fluid dynamics, CFD)建模的视角分析了生物质热化学转化过程。首先在欧拉-拉格朗日框架内给出了颗粒相和流体相的演化方程。接着,针对常见的生物质气流床和流化床转化过程(如热解、气化等),概... 详细信息

从颗粒多相流计算流体力学(computational fluid dynamics, CFD)建模的视角分析了生物质热化学转化过程。首先在欧拉-拉格朗日框架内给出了颗粒相和流体相的演化方程。接着,针对常见的生物质气流床和流化床转化过程(如热解、气化等),概述了文献中特别是本课题组在CFD模拟方面的研究进展。最后,针对生物质颗粒偏大的特性,综述了热厚模型的发展现状并介绍了课题组建立的一维和多维热厚模型。

关键词：生物质热化学转化欧拉-拉格朗日方法热厚模型

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生物质流化床热化学转化模拟研究专题序

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力学与实践 2022年第4期44卷 818-819页

作者：库晓珂王金张润辉浙江大学工程力学系杭州310027

生物质具有可再生、可产油产气、全生命周期CO近零排放等优点,而且其原料来源广泛、利用方式与煤炭接近,可用于发电或家用取暖设施,是未来全球化石能源的重要替代品之一。高效开发利用生物质能,对于促进能源供给结构低碳转型,缓解能源紧... 详细信息

生物质具有可再生、可产油产气、全生命周期CO近零排放等优点,而且其原料来源广泛、利用方式与煤炭接近,可用于发电或家用取暖设施,是未来全球化石能源的重要替代品之一。高效开发利用生物质能,对于促进能源供给结构低碳转型,缓解能源紧张,助力实现“双碳”目标都有着重要意义。

关键词：能源供给结构全生命周期热化学转化生物质能近零排放化石能源低碳转型替代品

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减小圆锥滚子轴承套圈锥度变形的方法

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哈尔滨轴承 2009年第2期30卷 29-31页

作者：张润慧李磊周保鑫哈尔滨轴承集团公司热处理分厂黑龙江哈尔滨150036 哈尔滨轴承集团公司技术中心黑龙江哈尔滨150036

圆锥轴承套圈淬火后易产生锥度变形,通过改变摆放方法,使淬火时冷却均匀,减少锥度变形,大大降低因锥度变形率超差而造成的废品。

关键词：圆锥轴承套圈淬火冷却锥度变形

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Immunization with a recombinant 58-kilodalton antigen from Baylisascaris schroederi induces protective immunity against larval migration of *** in mice

Immunization with a recombinant 58-kilodalton antigen from B...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Xie Yue,Gu Xiaobin,Fu Yan,zhang runhui,Zheng Wanpeng,Peng Xuerong,Wang Shuxian, Yang Guangyou~* Department of Parasitology,College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China

Baylisascaris schroederi(Nematoda:Ascaridida) is the leading cause of death from primary and secondary infection in wild and captive giant pandas due to a high infection rate and the pathogenic consequences of visceral larval migrans(VLM).To date,little was known about how protective immunity to *** infection could be *** this study,we have identified a cDNA encoding B. schroederi 58-kDa(Bs58) antigen(L2,L3 and adult stages) and evaluated the immune effect of the Escherichia coli-expressed recombinant protein(rBs58) in *** analysis showed that Bs58 has no similarity at the amino acid level to mammalian proteins but has some similarity to those of the filarial parasites and Caenorhabditis elegans gene ***,an intense expression of endogenous As24 in the body wall,gut epithelium,ovary and uterus of adult female worms was determined by *** addition,Bs58 homologues were found to be expressed in human,swine and dog *** of mice in three consecutive doses with rBs58 in Freund’s Complete Adjuvant (FCA) results in protection against challenge infections as manifested by a 69%reduction(P<0.001) in recovery compared with that in the non-treated group at day 7 *** obtained from immune protected mice showed a significantly increased in the level of the total immunoglobulin G(IgG) (p<0.0001) but had no IgE and IgM *** of the IgG subclasses showed a higher level of IgG1(p0.0001) and a lower level of IgG2a(p<0.005) antibody response in the sera of the immunized mice,suggesting that protection was associated with a typeⅡimmune *** immunization trial results in a *** model suggest the possibility of using rBs58 to develop a useful vaccine candidate antigen for parasitic diseases caused by ascarid nematodes in giant pandas and other animals.

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渗碳热处理缺陷分析

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哈尔滨轴承 2009年第3期30卷 31-32,50页

作者：张润慧张延成宋立波哈尔滨轴承集团公司热处理分厂黑龙江哈尔滨150036

详细分析渗碳热处理过程中的各种缺陷,指出产生缺陷的原因,提出了预防措施,在生产过程中取得良好效果。

关键词：渗碳温度热处理浓度缺陷预防措施

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Molecular characterization and phylogenetic analysis of ascarid nematodes from Ailuropoda melanoleuca and other twenty captive wild mammals based on mitochondrial and nuclear sequences

Molecular characterization and phylogenetic analysis of asca...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Li Yan,Xie Yue,Gu Xiaobin,Fu Yan,zhang runhui, Zheng Wanpeng,Peng Xuerong,Wang Shuxian,Yang Guangyou~* Department of Parasitology,College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China

Although ascarid nematodes are the most important parasites of wild animals with public health concern,most ascarids from wild animals has not been studied at molecular *** classification and phylogenetic relationships among and within roundworms from twenty-one captive wild animals in zoo were studied by sequencing and analysis of some parts of the ribosome 18S and 28S genes and mtDNA 12S *** and identity analysis of the three genes showed that roundworms from these animals were distributed into five genus:Toxocara cati taxa,Toxocara canis,Toxascaris,Baylisascaris and Ascaris. Homology analyses indicated that 18S genes sequence were conserved among roundworms from all these 21 animals.28S genes showed variability among *** levels of 12S genes sequences ranged from 11.7%to 12.8%between Toxocara cati and Toxocara canis indicating that mt 12S gene appeared variability among intraspecies and *** relationship were inferred by three methods,NJ/MP/ML based on the datas of rDNA,12S gene and combined three *** tree suggested that the ascarids of 21 species wild animals were classified into two families,four genus and seven *** alternative topologies showed that each clade was supported by high bootstrap value. Meanwhile,the combined trees suggested that the genetic relationships of Baylisascaris ailuri are closer to Baylisascaris transfuga than those of Baylisascaris schroederi and received higher support(94%).This study has provided useful molecular markers for the epidemiological investigation of roundworms from wild animals.

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Combining Two Methods to Molecular Cloning and Sequence Analysis of the Tp1 Gene from Taenia pisiformis

Combining Two Methods to Molecular Cloning and Sequence Anal...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Chen Lin,Yang DeYing,Gu XiaoBin,Yan Ning,Xie Yue,Fu Yan,zhang runhui,Zheng WanPeng, Yang GuangYou~* Department of Parasitology,College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China.

In order to clone the Tp1 gene of Taenia pisiformis,according to a unigene similar to Ts1 gene (EU009656.1) which come from transcriptomes sequencing result cooperated with Beijing Genomics Institute,specific primers(1F1 and 1F2) were designed to amplify 3’-end of Tp1 gene using rapid-amplification of cDNA ends(RACE).After that,Based on the unigene similar to Ts1 gene and initiator sequence of Ts1 cDNA,we designed the downstream primer(1R) and upstream primer(1FO) respectively to amplify the 5’end of Tp1,and the full length of the Tp1 gene was *** then, specific primers were designed according to the full length of the Tp1 *** sequence(removal the initiator 39 bp) of the Tp1 gene was successfully amplified by RT-PCR,and cloned into pMD19-T vector. The recombinant plasmid pMD19-T-Tp1 was identified by PCR,restriction enzymes(BamH I+Nco I). Then the gene was analyzed by bioinformatics *** results showed that a 684 bp complete open reading frame of 227 amino acids with Mr 25.801kDa and PI 6.158 was discovered.A promoter located from 81 to 131 was *** promoter score was *** 9 possible B cell epitopes covered the whole polypeptide chains of Tp1 CDS regions,where hydrophilicity regions took the most *** had better hydrophilicity,flexible regions and higher antigen *** Tp1 gene was predicted to be situated in *** had 11 potential phosphorylation *** phosphorylation sites were composed of 4 serine and 7 *** secondary structure was analysised by Garnier-Robson and Chou-Fasman tool of DNAstar 6.***.84.6%alpha helix,1.32%beta sheet,3.07%beta turn and 1.14%random coil were predicted by the former,while 57.0%alpha helix,22.4%beta sheet and 15.8%beta turn by the later respectively. The main 9 flexible regions located at 6-47,50-57,64-70,91-96,100-108,122-140,149-161,187-199,and *** tertiary structure had not been gained by the way of homology modelling of molecular *** results provided

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