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Application of Human Adenovirus Genotyping by Phylogenetic Analysis in an Outbreak to Identify Nosocomial Infection

Virologica Sinica 2021年第3期36卷 393-401页

作者： Chuanyu Yang Chunmei zhu Yuan Qian Jie Deng Baoyuan Zhang runan zhu Fang Wang Yu Sun Dongmei Chen Qi Guo Yutong Zhou Lei Yu Ling Cao Linqing Zhao Laboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of PediatricsBeijing 100020China Department of Respiratory Medicine Affiliated Children's HospitalCapital Institute of PediatricsBeijing 100020China Hospital Infection-Control Department Affiliated Children’s HospitalCapital Institute of PediatricsBeijing 100020China

Nosocomial infections are common in pediatric patients and can be fatal in infants and immunocompromised patients. In September 2018, a high positive rate of human adenovirus HAdV was occurred among hospitalized children in the Children's Hospital Affiliated to the Capital Institute of Paediatrics in Beijing. To investigate whether this outbreak of HAdV was related to nosocomial infections or the result of community infections, we collected respiratory specimens from patients with acute respiratory infections in a respiratory ward during June to December 2018, and screened for respiratory viruses. Among 1,840 cases included, 95(5.2%, 95/1840) were positive for HAdV and 81 were genotyped based on phylogenetic analysis, including seven as HAdV-1(8.6%), 30 HAdV-3(37.0%), two HAdV-6(2.5%), and 42 HAdV-7(51.9%). More HAdV-positive samples were collected in August(4.7%, 12/255), September(15.0%, 41/274) and October(6.9%, 17/247), with a peak in September 2018. By combining the results of HAdV phylogenetic analysis with clinical data of patients, there were 77 cases(4.2%, 77/1840;81.1%, 77/95) excluded from nosocomial infections, eight cases representing possible infections transmitted by visitors or attending parents, three cases without sequences that might have been due to infection transmitted by roommates positive for HAdV, one case of a roommate without an HAdV sequence, and six cases that shared highly homologous sequences with those of their roommates, for which nosocomial infections might be considered. In conclusion, genotyping of HAdVs based on phylogenetic analysis combined with clinical information provides a powerful method to distinguish nosocomial infections from community acquired infection, especially when tracing the origins of nosocomial infections.

关键词： Acute respiratory tract infection Human adenovirus(HAdV) Phylogenetic analysis Nosocomial infection

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Human rhinovirus C infection is associated with asthma in children determined by x TAG respiratory viral panel FAST

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Virologica Sinica 2017年第2期32卷 171-174页

作者： Dongmei Chen Qinwei Song runan zhu Yuan Qian Yu Sun Jie Deng Fang Wang Yaxin Ding Run Tian Chuanhe Liu Wenjing zhu Linqing Zhao Laboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of Pediatrics Division of Respiratory Diseases Affiliated Children's HospitalCapital Institute of Pediatrics

Dear Editor,Cumulative evidence supports the role of early-life viral infections,especially respiratory syncytial virus(RSV)and human rhinovirus(HRV),as major antecedents of childhood asthma(Lemanske,2002;Jackson et al.,2008).In this study,the x TAG respiratory viral panel FAST(RVP FAST)assay,a multiplex polymerase chain reaction(PCR)-based method(Arens et al.,2010;BaladaLlasat et al.,2011;Gharabaghi et al.,2011;Selvaraju,2012),was used to investigate the association of infec-

关键词： HRV Human rhinovirus C infection is associated with asthma in children determined by x TAG respiratory viral panel FAST TAG

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Virus profile in children with acute respiratory infections with various severities in Beijing, China

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Chinese Medical Journal 2014年第21期127卷 3706-3711页

作者： zhu runan Song Qinwei Qian Yuan Zhao Linqing Deng Jie Wang Fang Sun Yu Laboratory of Virology Capital Institute of Pediatrics Beijing 100020 China

Background Acute respiratory infection （ARI） is one of the most common infectious diseases in infants and young children *** study aimed to determine the virus profile in children with ARI presenting with different *** Clinical specimens collected from children with ARI in Beijing from September 2010 to March 2011 were investigated for 18 respiratory viruses using an xTAG Respiratory Viral Panel Fast （RVP Fast） *** Pearson chisquare analysis was used to identify statistical *** Of 270 cases from three groups of ARI patients,including Out-patients,In-patients and patients in the intensive care unit （ICU）,viruses were detected in 176 （65.2％） specimens with the RVP Fast *** viral detection rate from the Out-patients group （50.0％） was significantly lower than that from the In-patients （71.1％） and ICU-patients （74.4％） *** virus distribution was different between the Out-patients group and the other hospitalized groups,while the virus detection rate and distribution characteristics were similar between the In-patients and ICU-patients *** coinfection rates of the Out-patients group,the In-patients group,and the ICU-patients group were 15.6％,50.0％ and 35.8％,*** addition to respiratory syncytial virus （RSV） and adenovirus （ADV）,human rhinovirus （HRV） was frequently detected from children with serious illnesses,followed by human metapneumovirus （hMPV）,human bocavirus （HBoV） and *** virus 3 （PIV3） was detected in children with lower respiratory illness,but rarely from those with serious illnesses in the ICU-patient *** In addition to so-called common respiratory viruses,virus detection in children with ARI should include those thoucht to be uncommon respiratory viruses,especially when there are severe ARI-related clinical illnesses.

关键词： respiratory viruses acute respiratory infections co-infection children xTAG Respiratory Viral Panel Fast assay

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肠道病毒D68的研究进展

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中华实验和临床病毒学杂志 2019年第4期33卷 440-443页

作者：胡亚芳朱汝南首都儿科研究所病毒研究室儿童病毒病病原学北京市重点实验室北京100020

肠道病毒D68(enterovirus D68,EV-D68)首次于1962年被分离,随后少有报道。近10年,随着其基因多样性迅速增加,EV-D68在世界范围内的检出率开始增高。自2014年EV-D68在美国、加拿大及欧洲暴发流行以来,更多研究报道了其可引起严重的呼吸... 详细信息

肠道病毒D68(enterovirus D68,EV-D68)首次于1962年被分离,随后少有报道。近10年,随着其基因多样性迅速增加,EV-D68在世界范围内的检出率开始增高。自2014年EV-D68在美国、加拿大及欧洲暴发流行以来,更多研究报道了其可引起严重的呼吸道疾病和神经系统并发症,甚至死亡。然而,EV-D68的致病机制仍不明确,目前也没有用于预防和治疗的疫苗以及特效抗病毒药物。因此,持续监测和快速诊断EV-D68感染将更加有利于应对可能发生的暴发流行。本文综合论述了EV-D68的生物学特征、临床表现、流行情况等。

关键词：肠道病毒68型生物学特征临床表现流行病学

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流感病毒检测方法研究进展

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中华实用儿科临床杂志 2019年第2期34卷 102-106页

作者：朱汝南钱渊首都儿科研究所病毒研究室儿童病毒病原学北京市重点实验室北京100020

流感是人类呼吸道感染中常见且流行范围最广的疾病之一。流感不仅对易感和高危人群造成严重威胁,同时也对公共卫生造成重要影响。流感病毒感染的快速、准确诊断对于及时给予特异的抗病毒治疗、有效控制疫情、降低季节性流行和大流行期... 详细信息

流感是人类呼吸道感染中常见且流行范围最广的疾病之一。流感不仅对易感和高危人群造成严重威胁,同时也对公共卫生造成重要影响。流感病毒感染的快速、准确诊断对于及时给予特异的抗病毒治疗、有效控制疫情、降低季节性流行和大流行期间与流感病毒感染有关的发病率和病死率至关重要。现对目前可用于人类流感病毒感染的诊断方法(包括病毒分离、抗原检测、核酸扩增等)及为了克服一些传统检测方法的局限性而正在研发的新的诊断方法进行综述,以供临床医师参考。

关键词：流感病毒诊断病毒分离抗原检测核酸扩增快速诊断

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Expression of WU polyomavirus capsid protein VP2 for the preliminary serology study in Beijing,China

Expression of WU polyomavirus capsid protein VP2 for the pre...

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第二届中国临床微生物学大会暨微生物学与免疫学三江论坛

作者： Linqing ZHAO Nina ZHANG Yuan QIAN runan zhu Jie DENG Fang WANG Yu SUN Liying LIU Capital Institute of Pediatrics

Since the pathogenicity of several human polyomaviruses have been clarified,it has become urgent to determine the epidemiology of WU polyomavirus（WUPyV）.No cell line is sensitive to WUPyV,so the prokaryotic expression system with 6×histidines tag was used to express the recombinant WUPyV VP2 in *** BL21（DE3） under optimized expression conditions,and the purified recombinant VP2 was used as antigen to examine serum antibodies against WUPyV VP2 in Beijing population by Western blotting to learn the extent of WUPyV infection in *** results showed that the recombinant VP2 was expressed more successfully in TB medium than in LB,then the protein was harvested,affinity-purified and used as *** sero-positivity of antibodies IgG against WUPyV VP2 was 52%in the population detected,and high degree sero-positivity observed in the age group younger than 6m,which is similar to that of adults（＞18y）,suggested antibodies against WUPyV VP2 were maternally *** the sero-positivity decreased to a nadir in the next age group（6m to ＜ly）,and then steadily increased to a peak（83.3%） followed with a plateau of about 50%,which may indicate that primary infection occurs mainly at early childhood,and the infection rates increased rapidly in children between 1 and 4 years of *** conclusion,the sero-prevalence of antibodies against VP2 by using recombinant WUPyV VP2 expressed in TB medium demonstrated that WUPyV is widespread in Beijing.

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Human parainfluenza virus type 4 infections in infants and young children with acute respiratory infections in Beijing

Human parainfluenza virus type 4 infections in infants and y...

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中华医学会第十七次全国儿科学术大会

作者： Linqing Zhao,Yuan Qian,Fang Wang,runan zhu,Jie Deng,Yu Sun,Yaxin Ding,Minghui Song Laboratory of Virology,Capital Institute of Pediatrics

Human parainfluenza viruses(HPIVs) are common viral pathogens of lower or upper respiratory tract infections for humans in any age,especially in infants and young *** of the difficulties in isolating virus from clinical specimens and the lack of routine diagnostic test,the clinical significance of human parainfluenza virus type 4(HPIV-4) is not well defined as that of parainfluenza virus types *** investigate the impact of HPIV-4 to acute respiratory infections in

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Viruses associated with hand,foot and mouth disease in children in Beijing during 2007 to 2011

Viruses associated with hand,foot and mouth disease in child...

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2012全国临床微生物与感染免疫学术研讨会

作者： zhu runan,Qian Yuan,Deng Jie,Zhao Linqing,Sun Yu,Wang Fang,Deng Li,Zhang Yanling Department of Virology,Capital Institute of Pediatrics

Hand,foot and mouth disease(HFMD) is one of the most common infectious diseases in young children, especially those under 5 years *** 71(EV71) and coxsackievirus A 16(CA16) are the two major causative organisms of *** order to understand the etiological agent of HFMD in children in Beijing, throat swabs and/or vesicle fluids were collected from children diagnosed clinically as HFMD from March to October each year during 2007 to ***-nested PCR using special primers of 5’UTR and VPl region of CA16 and EV71 to identify the types of virus,virus isolation and sequence analysis of VPl gene were *** children were aged from 1 month to 15 years old,and,93.6%of them were younger than 5 years *** culture and reverse-transcription polymerase chain reactions were performed for virus isolation and identification.A total of 837 clinical samples collected from children with HFMD were inoculated in Vero cells and 494 strains were isolated(58.9%),including 254 of CA16 and 239 of *** isolation rate from vesicle fluids was 68.8%.CA16 was the dominant virus in 2007 and 2009,while EV71 was the major cause in ***71 and CA16 were circulating equally in 2010 and *** EV71 strains,but no CA16 were isolated from clinical samples collected from children with severe cases of *** sequences of VP1 region from 25 of CA16 isolates and 31 of EV71 isolates were analyzed and genotyped by phylogenetic *** and amino acid identities of CA16 VP1 were 90.0%-100%and 98.3%-100%,respectively and those of EV71 VP1 were 94.4%-99.0%and 97.0%-100%.Phylogenetic analysis based on nucleotide sequence indicated that all EV71 isolates analyzed belonged to subgenotype C4 and the evolution distance ***-related with the length of interval of time when the virus was *** CA16 analyzed belonged to genotype B,and further divided into subgenotype B2a and B2b. CA16 strains isolated in 2007 were divided into B2b and strains from 2008

关键词： Enterovirus 71 Coxasckievirus A16 Hand foot and mouth disease

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Viruses Associated with Hand,Foot and Mouth Disease in Children in Beijing During 2007 to 2011

Viruses Associated with Hand,Foot and Mouth Disease in Child...

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中华医学会第十七次全国儿科学术大会

作者： runan zhu,Yuan Qian,Jie Deng,Linqing Zhao,Yu Sun,Fang Wang,Li Deng,Yanling Zhang Department of Virology,Capital Institute of Pediatrics

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转型高校“观念转变”影响因素及其作用机理探析——基于转型发展试点院校的调查

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职教论坛 2019年第10期35卷 109-113页

作者：闫志军朱如楠河北科技师范学院

观念是静态存在,具有较强的稳定性;观念转变是动态过程,意味着对传统的突破。对于每次重大改革来说,观念转变都绝非易事。在地方本科高校转型发展改革进程中,改革参与者观念的转变受到多重因素的影响,通过质性访谈、问卷调查和DEMATEL... 详细信息

观念是静态存在,具有较强的稳定性;观念转变是动态过程,意味着对传统的突破。对于每次重大改革来说,观念转变都绝非易事。在地方本科高校转型发展改革进程中,改革参与者观念的转变受到多重因素的影响,通过质性访谈、问卷调查和DEMATEL量化分析,最后确定主客观两大维度共计9项因素,其中核心因素3项、原因性因素5项、结果性因素4项。转型高校在推动观念转变过程中,应围绕高中心度因素重点解决主要矛盾,根据原因性因素建立动力机制,注意客观维度实践性因素的作用,辩证分析观念转变的具体问题,合理发挥各项因素影响力。

关键词：转型发展观念转变影响因素作用机理

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