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More common RNAemia in the early stage of severe SARS-CoV-2 BF.7.14 infections in pediatric patients

Biosafety and Health 2024年第1期6卷 5-11页

作者： Yu Sun runan zhu Yang Pan Ri De Shuang Liu Liping Jia Bing Lv Xiaoyun Li Dongmei Chen Yao Yao Dong Qu Daitao Zhang Linqing Zhao Laboratory of Virology Bejing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of PediatricsBejing 100020China Institute for Infectious Disease and Endemic Disease Control Bejing Center for Disease Control and Prevention Beijing 100013China Department of Intensive Care Unit Affiliated Children's Hospital of Capital Institute of PediatricsBeijing 100020China

The risk factors of severe infections in children during the severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)outbreak in Beijing remain elusive.SARS‐CoV‐2‐positive children admitted to the intensive care unit(ICU)with collected plasma specimens were enrolled and screened for common pathogens using capillary electrophoresis‐based multiplex PCR from December 12,2022,to January 24,2023.The SARS‐CoV‐2 subvariants were identified using next‐generation sequencing.Plasma was positive for two(positive;P),one(suspicious;S),or no(negative;N)SARS‐CoV‐2 genes were classified as plasmatic RNA‐positive(RNAemia;P+S)or without RNAemia(N).Clinical and laboratory data of the enrolled cases were then collected and analyzed.The 34 enrolled children included 26 males and 24 younger than three years.All were negative for other respiratory pathogens.BF.7.14(18/29)was the predominant subvariant.Viral loads in respiratory specimens,hours from symptom onset to the first respiratory specimen collection(time‐variable),with comorbidities and BF.7.14 and BA.5.2 distributions were significantly different in P vs.N and RNAemia vs.without RNAemia group.Among most cases,the T lymphocyte ratios decreased,while the cytokine level and the B lymphocyte ratio increased.The time variables were 2.22±2.05 and 4.00±2.49 days in BF.7.14 and BA.5.2 infections,respectively.In conclusion,SARS‐CoV‐2 was more likely to cause severe infections among males aged≤3 years old with comorbidities during the SARS‐CoV‐2 outbreak in Beijing,while RNAemia is more common in children at the early stage of severe BF.7.14 infections,and most had high cytokine levels and B‐cell activation.

关键词： Severe SARS‐CoV‐2 infection Pediatric patients Risk factor RNAemia BF.7.14

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Radiated noise measurement method of underwater low noise target by a vertical nested array

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Chinese Journal of Acoustics 2024年第1期43卷 98-112页

作者： LI Xuegang JIANG Guoqing SUN Guocang HUA runan zhu Jie Wuhan Second Ship Design and Research Institute Wuhan 430205 School of Marine Science and Technology Northwestern Polytechnical UniversityXi'an 710072

A radiated noise measurement method of underwater acoustic targets with relatively low power by a vertical nested array is studied. Firstly, the array gain requirements for different frequency bands are obtained through comparative analysis of the background noise level and the radiated noise level of the target. Secondly, an ultra wideband constant-beamwidth beamforming method with high robustness is established for the waveguide environment based on convex optimization. The radiated noise measurement and the following spatial array signal processing procedure is formulated. Finally, a vertical nested array measurement system is developed, and the radiated noise measurement method is verified using lake test data. Experimental results show that the radiated noise measurement method has large working bandwidth, high robustness and low measurement uncertainty of about 2 dB.

关键词： underwater method bands

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“双一流”和“部省合建”双重战略下的西部高水平大学建设——以民族八省区9所高校为例

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教育学术月刊 2024年第1期 16-25页

作者：李明忠朱如楠李倩河北大学教育学院河北保定071002

“世界一流、中国特色、西部风格”高水平大学是“双一流”和“部省合建”双重战略对民族八省区9所高校提出的建设目标与时代使命。在双重战略导向下,9所高校进一步明确办学目标及特色、构建学科生态系统、优化师资队伍结构、完善内部... 详细信息

“世界一流、中国特色、西部风格”高水平大学是“双一流”和“部省合建”双重战略对民族八省区9所高校提出的建设目标与时代使命。在双重战略导向下,9所高校进一步明确办学目标及特色、构建学科生态系统、优化师资队伍结构、完善内部治理体系,探索出一条特色鲜明的西部高水平大学建设之路,同时也存在政策落实不到位、办学实力较弱、陷入路径依赖困境等问题。因此,西部高水平大学建设要想取得高质量发展,需要内外联动形成合力,一是进一步加大各级政府政策支持和资源倾斜,二是加强高校自身建设的内生动力和特色发展。

关键词：西部高水平大学 “双一流” “部省合建” 民族八省区

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Application of Human Adenovirus Genotyping by Phylogenetic Analysis in an Outbreak to Identify Nosocomial Infection

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Virologica Sinica 2021年第3期36卷 393-401页

作者： Chuanyu Yang Chunmei zhu Yuan Qian Jie Deng Baoyuan Zhang runan zhu Fang Wang Yu Sun Dongmei Chen Qi Guo Yutong Zhou Lei Yu Ling Cao Linqing Zhao Laboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of PediatricsBeijing 100020China Department of Respiratory Medicine Affiliated Children's HospitalCapital Institute of PediatricsBeijing 100020China Hospital Infection-Control Department Affiliated Children’s HospitalCapital Institute of PediatricsBeijing 100020China

Nosocomial infections are common in pediatric patients and can be fatal in infants and immunocompromised patients. In September 2018, a high positive rate of human adenovirus HAdV was occurred among hospitalized children in the Children's Hospital Affiliated to the Capital Institute of Paediatrics in Beijing. To investigate whether this outbreak of HAdV was related to nosocomial infections or the result of community infections, we collected respiratory specimens from patients with acute respiratory infections in a respiratory ward during June to December 2018, and screened for respiratory viruses. Among 1,840 cases included, 95(5.2%, 95/1840) were positive for HAdV and 81 were genotyped based on phylogenetic analysis, including seven as HAdV-1(8.6%), 30 HAdV-3(37.0%), two HAdV-6(2.5%), and 42 HAdV-7(51.9%). More HAdV-positive samples were collected in August(4.7%, 12/255), September(15.0%, 41/274) and October(6.9%, 17/247), with a peak in September 2018. By combining the results of HAdV phylogenetic analysis with clinical data of patients, there were 77 cases(4.2%, 77/1840;81.1%, 77/95) excluded from nosocomial infections, eight cases representing possible infections transmitted by visitors or attending parents, three cases without sequences that might have been due to infection transmitted by roommates positive for HAdV, one case of a roommate without an HAdV sequence, and six cases that shared highly homologous sequences with those of their roommates, for which nosocomial infections might be considered. In conclusion, genotyping of HAdVs based on phylogenetic analysis combined with clinical information provides a powerful method to distinguish nosocomial infections from community acquired infection, especially when tracing the origins of nosocomial infections.

关键词： Acute respiratory tract infection Human adenovirus(HAdV) Phylogenetic analysis Nosocomial infection

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新型电力系统下电弧故障诊断技术及发展趋势

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电测与仪表 2024年第2期61卷 1-9页

作者：秦译为张蓬鹤宋如楠祝毛宁中国电力科学研究院北京100192

确保电力能源安全稳定供应是构建新型电力系统的前提,用电安全领域故障诊断技术需要不断革新和进步,以适应电力系统的新需求。故障电弧无法精准诊断作为制约电气火灾监测预防的主要原因,严重威胁到供用电安全。文中分析了基于不同原理... 详细信息

确保电力能源安全稳定供应是构建新型电力系统的前提,用电安全领域故障诊断技术需要不断革新和进步,以适应电力系统的新需求。故障电弧无法精准诊断作为制约电气火灾监测预防的主要原因,严重威胁到供用电安全。文中分析了基于不同原理的故障电弧诊断方法,介绍了故障电弧试验检测技术及相关标准,并提出了未来可能的研究方向,最后总结了新型电力系统下电弧故障诊断面临的技术挑战和发展趋势。

关键词：故障电弧新型电力系统诊断特征试验检测

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Human rhinovirus C infection is associated with asthma in children determined by x TAG respiratory viral panel FAST

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Virologica Sinica 2017年第2期32卷 171-174页

作者： Dongmei Chen Qinwei Song runan zhu Yuan Qian Yu Sun Jie Deng Fang Wang Yaxin Ding Run Tian Chuanhe Liu Wenjing zhu Linqing Zhao Laboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of Pediatrics Division of Respiratory Diseases Affiliated Children's HospitalCapital Institute of Pediatrics

Dear Editor,Cumulative evidence supports the role of early-life viral infections,especially respiratory syncytial virus(RSV)and human rhinovirus(HRV),as major antecedents of childhood asthma(Lemanske,2002;Jackson et al.,2008).In this study,the x TAG respiratory viral panel FAST(RVP FAST)assay,a multiplex polymerase chain reaction(PCR)-based method(Arens et al.,2010;BaladaLlasat et al.,2011;Gharabaghi et al.,2011;Selvaraju,2012),was used to investigate the association of infec-

关键词： HRV Human rhinovirus C infection is associated with asthma in children determined by x TAG respiratory viral panel FAST TAG

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2023年春季北京地区儿童急性呼吸道感染病原谱分析

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中华儿科杂志 2024年第2期62卷 159-164页

作者：孙宇朱汝南王芳陈冬梅郭琪周禹彤姚尧贾立平赵林清首都儿科研究所病毒学研究室儿童病毒病病原学北京市重点实验室北京100020

目的探讨北京地区儿童急性呼吸道感染(ARI)的病原谱。方法采用横断面研究,收集2023年2月6日(第6周)至5月28日(第21周)首都儿科研究所附属儿童医院因ARI就诊的9284例患儿呼吸道标本。根据标本来源,分为急诊就诊的流感样病例组、门诊ARI... 详细信息

目的探讨北京地区儿童急性呼吸道感染(ARI)的病原谱。方法采用横断面研究,收集2023年2月6日(第6周)至5月28日(第21周)首都儿科研究所附属儿童医院因ARI就诊的9284例患儿呼吸道标本。根据标本来源,分为急诊就诊的流感样病例组、门诊ARI组及住院ARI组。流感样病例组进行流感病毒(Flu)及人偏肺病毒(HMPV)核酸检测;门诊ARI组进行Flu、呼吸道合胞病毒(RSV)、腺病毒、副流感病毒(PIV)核酸检测;住院ARI组进行Flu、RSV、HMPV、腺病毒、人博卡病毒(HBoV)、鼻病毒、PIV、冠状病毒、肺炎支原体(Mp)及衣原体核酸检测。结果流感样病例组320例患儿中男192例、女128例,就诊年龄4.7(3.6,6.9)岁,Flu A阳性117例(36.6%),其中甲型流感病毒H1N1亚型(H1N1)55例(47.0%),甲型流感病毒H3N2亚型(H3N2)62例(53.0%),第6~9周以H1N1为主(10.0%~50.0%),第10~16周以H3N2为主(15.0%~90.0%);HMPV阳性13例(4.1%),第15周检出率为5.0%(1/20),第20周达30.0%(6/20)。门诊ARI组7573例患儿中男4131例、女3442例,就诊年龄4.0(2.1,5.3)岁,阳性检出率RSV为32.9%(2491/7573),Flu A为12.1%(915/7573);第6~14周以Flu A为主(23.2%~74.7%),第15周开始以RSV为主,阳性检出率为24.8%(36/145)。住院ARI组1391例患儿中男804例、女587例,就诊年龄3.3(0.4,5.8)岁,阳性检出率鼻病毒为18.7%(260/1391)、RSV为12.4%(173/1391)、Flu A为10.2%(142/1391)[H1N1116例(81.7%)、H3N226例(18.3%)]及HMPV为3.1%(43/1391)。第7周开始检出H1N1[10%(6/60)],第11周达高峰[31.8%(21/66)],第8周开始检出H3N2[1.5%(1/68)]。住院ARI组Flu阳性患儿H1N1阳性检出率为81.7%(116/142)。第12周开始检出RSV,阳性检出率为1.3%(1/80),第19周达30.4%(35/115);第21周HMPV阳性检出率为12.1%(14/116)。结论北京地区2023年春季(2至5月)ARI患儿中首先出现Flu流行,前期以H1N1为主,后期以H3N2为主,RSV出现滞后流行,同时需密切关注HMPV。应加强对门诊监测,提前发出流行预警。

关键词：儿童急性呼吸道感染病原

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Leaf phenotypic variation in natural populations of Carpinus tschonoskii in China

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林业研究:英文版 2023年第5期34卷 1591-1602页

作者： runan Zhao Xiaojie Chu Qianqian He Wei Liu Zunling zhu College of Landscape Architecture Nanjing Forestry UniversityNanjing 210037People’s Republic of China Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037People’s Republic of China College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004People’s Republic of China

Carpinus tschonoskii Maxim.exhibits rich leaf phenotypic variation and various leaf shapes,but few studies show why leaf phenotypic traits have such a large variation.Basic morphological markers may provide guidance for studying plant genetic variation and species protection and utilization.To study leaf phenotypic variations and the relationship between variation characteristics and climatic and geographical factors,phenotypic traits among natural populations were investigated.Results revealed that leaf phenotypes varied significantly among and within populations.Some populations had higher phenotypic diversity,while others had lower phenotypic diversity.Among the phenotypic traits,leaf area and petiole length had the most variation.Leaf index and primary lateral veins were the most stable phenotypes,which may be important reference indexes for phenotype identification in field investigations.There was a strong consistency between leaf phenotypic traits and geographical location.Plants in high latitudes tend to have longer leaves,and plants in low temperatures tend to have longer leaves and larger leaf perimeter.In addition,plants in areas with less rainfall have longer petioles.The 13 populations of C.tschonoskii can be divided into four branches by cluster analysis,and the results show a good relationship with the geographical location of each population.Additionally,some populations geographically isolated also had unique leaf phenotypes.

关键词： Carpinus tschonoskii Leaf phenotypes Phenotypic plasticity Environmental factors Conservation measures

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人呼吸道合胞病毒北京地区A亚型ON1基因型地方株感染A549细胞转录组学分析

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中华微生物学和免疫学杂志 2024年第2期44卷 128-133页

作者：王凤杰江明礼郭琪李晓芸孙宇朱汝南赵林清首都儿科研究所病毒学研究室儿童病毒病病原学北京市重点实验室北京100020

目的应用转录组测序方法,分析北京地区人呼吸道合胞病毒(respiratory syncytial virus,RSV)A亚型优势流行基因型ON1地方株感染A549细胞后差异表达基因,为RSV防治提供潜在靶点。方法选用已经过全基因组测序确定为RSV A亚型ON1基因型的地... 详细信息

目的应用转录组测序方法,分析北京地区人呼吸道合胞病毒(respiratory syncytial virus,RSV)A亚型优势流行基因型ON1地方株感染A549细胞后差异表达基因,为RSV防治提供潜在靶点。方法选用已经过全基因组测序确定为RSV A亚型ON1基因型的地方株(61397-ON1)感染A549细胞,提取总mRNA,通过转录组测序筛选出与未感染的A549细胞为对照的差异表达基因,对其进行GO分析、KEGG通路分析,同时随机选择6个差异表达倍数大于2倍的基因进行qRT-PCR验证。结果以未感染的A549细胞为对照,筛选出1632个差异表达基因,其中807个基因表达上调,825个基因表达下调。差异基因主要参与细胞因子反应以及MAPK级联反应正向调控等免疫应答相关生物过程,并在MAPK信号通路、NOD样受体信号通路、p53信号通路、TNF信号通路、IL-17信号通路及NF-κB信号通路发生了富集。选择的6个差异表达基因qRT-PCR验证结果与转录组数据趋势一致。结论RSV A亚型ON1基因型毒株感染A549细胞后的差异表达基因主要参与细胞因子应答及免疫相关信号通路,为RSV致病的分子机制及防治策略研究提供基础资料。

关键词：呼吸道合胞病毒地方株 ON1基因型转录组学分析差异表达基因

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精氨酸对引起采后蓝莓腐烂的链格孢靶标菌的抑制效果研究

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包装工程 2024年第3期45卷 91-98页

作者：王佳琦杨雪赵汝楠朱恂飞刘闯张爽严雪瑞沈阳农业大学食品学院沈阳110866 沈阳农业大学设施园艺教育部重点实验室沈阳110866

目的旨在筛选引起蓝莓采后腐烂的链格孢靶标菌株,并比较L-精氨酸和山梨酸钾对其抑制效果,为蓝莓采后链格孢腐烂病的防控提供前期基础。方法通过组织分离法结合单孢分离法进行致腐菌的分离,采用ITS序列分析进行属水平鉴定,采用ATP序列分... 详细信息

目的旨在筛选引起蓝莓采后腐烂的链格孢靶标菌株,并比较L-精氨酸和山梨酸钾对其抑制效果,为蓝莓采后链格孢腐烂病的防控提供前期基础。方法通过组织分离法结合单孢分离法进行致腐菌的分离,采用ITS序列分析进行属水平鉴定,采用ATP序列分析结合形态学观察进行种水平鉴定,采用平板抑菌法比较L-精氨酸和山梨酸钾的抑菌效果。结果本研究分离获得的链格孢菌均有致病性,分别属于3个不同种群,分别为细极链格孢(Alternaria tenuissima)、互隔交链孢(A.alternata)和云南铁杉链格孢(A.dumosa)。以菌株B20190623E3(A.tenuissima代表菌株)、菌株B20190623B1(A.alternata代表菌株)、菌株B20190623C1(A.dumosa代表菌株)为靶标菌,发现L-精氨酸对供试3株靶标菌的EC50值均低于山梨酸钾。结论供试链格菌株均有致病性,其中引起蓝莓采后腐烂的优势链格孢种群为细极链格孢。云南铁杉链格孢为引起蓝莓果实腐烂的新纪录种。筛选获得了3株链格孢代表靶标菌。L-精氨酸对引起蓝莓采后腐烂的链格孢菌具有较好的抑制效果。

关键词：蓝莓链格孢种群致病性 L-精氨酸抑菌效果

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