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Temporal and environmental factors drive community structure and function of methanotrophs in volcanic forest soils

Journal of Forestry Research 2024年第2期35卷 1-13页

作者： Rusong chai Hongjie Cao Qingyang Huang Lihong Xie Fan Yang Hongbin Yin Heilongjiang Forest Botanical Garden Harbin 150040People’s Republic of China 2 Institute of Natural Resources and Ecology Science Academy of Heilongjiang ProvinceHarbin 150040People’s Republic of China

Methanotrophs,organisms that obtain oxygen by oxidizing methane,are recognized as the only known biological sink for atmospheric CH_4,and forest soil methanotrophs play crucial roles in mitigating global *** succession patterns of methanotrophic communities and functions in Wudalianchi volcano forest soils could provide a basis for the study of evolutionary mechanisms between soil microorganisms,the environment,and carbon cycling of temperate forest ecosystems under climate *** this study,the characteristics and drivers of methanotrophic community structure and function of two volcanic soils at different stages of development are analyzed,including an old volcano and a new volcano,which most recently erupted 300 years and 17-19×10^(5)years ago,respectively,and a non-volcano hills as control,based on space for time substitution and Miseq sequencing and bioinformation *** results showed that CH_(4) fluxes were significantly higher in old-stage volcano forest soils than new-stage forest soils and non-volcano forest *** were significant differences in the community composition and diversity of soil methanotrophs from different volcano forest *** was the dominant genus in all soil ***,the relative abundance of Methylococcus,along with Clonothrix,Methyloglobulus,Methylomagum,Methylomonas and Methylosarcina,were the important genera responsible for the differences in methanotrophic community structure in different volcano forest *** relative abundance of methanotroph belonging toγ-proteobacteria was significantly higher than that belonging toα-proteobacteria(P<0.05).Chao1,Shannon and Simpson indices of soil methanotrophic community were significantly lower in new-stage volcanos and were significantly affected by bulk density,total porosity,p H,nitrate,dissolved organic carbon and dissolved organic *** were significant differences in community structure between new-stage and old-stage volcano

关键词： Methanotrophs pmo A Soil development stage Volcanoes Forest soils

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Aspect-Level Sentiment Analysis Based on Deep Learning

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Computers, Materials & Continua 2024年第3期78卷 3743-3762页

作者： Mengqi Zhang Jiazhao chai Jianxiang Cao Jialing Ji Tong Yi Department of Communication University of New South WalesSydney2052Australia Higher Vocational Education Publishing Division Higher Education PressBeijing100029China State Key Laboratory of Media Convergence and Communication Communication University of ChinaBeijing100020China Key Lab of Education Blockchain and Intelligent Technology Ministry of EducationGuangxi Normal UniversityGuilin541004China

In recent years,deep learning methods have developed rapidly and found application in many fields,including natural language *** the field of aspect-level sentiment analysis,deep learning methods can also greatly improve the performance of ***,previous studies did not take into account the relationship between user feature extraction and contextual *** address this issue,we use data feature extraction and deep learning combined to develop an aspect-level sentiment analysis *** be specific,we design user comment feature extraction(UCFE)to distill salient features from users’historical comments and transform them into representative user feature ***,the aspect-sentence graph convolutional neural network(ASGCN)is used to incorporate innovative techniques for calculating adjacency matrices;meanwhile,ASGCN emphasizes capturing nuanced semantics within relationships among aspect words and syntactic dependency ***,three embedding methods are devised to embed the user feature vector into the ASGCN *** empirical validations verify the effectiveness of these models,consistently surpassing conventional benchmarks and reaffirming the indispensable role of deep learning in advancing sentiment analysis methodologies.

关键词： Aspect-level sentiment analysis deep learning graph convolutional neural network user features syntactic dependency tree

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A Data-Driven Real-Time Trajectory Planning and Control Methodology for UGVs Using LSTMRDNN

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1292-1294页

作者： Kaiyuan Chen runqi chai runda Zhang Zhida Xing Yuanqing Xia Guoping Liu Vanke School of Public Health Institute for Healthy ChinaTsinghua UniversityBeijing 100084 School of Automation Beijing Institute of TechnologyBeijing 100081 Department of Electronic and Electrical Engineering Southern University of Science and TechnologyShenzhen 518055China

Dear Editor,This letter presents a novel data-driven trajectory planning and control scheme for the unmanned ground vehicles(UGVs).A recent work[1]has demonstrated the effectiveness of approximating the optimal state ... 详细信息

关键词： LSTM UGV neural

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Preparation of Fe-As alloys by mechanical alloying and vacuum hot-pressed sintering:microstructure evolution,mechanical properties,and mechanisms

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Rare Metals 2024年第8期43卷 3876-3892页

作者： Fei chai Fei-Ping Zhao Zhan Hu Shi-Yi Wen Ben-Hammouda Samia Ze-Lin Fu Xin-Ting Lai Yan-Jie Liang Xiao-Bo Min Li-Yuan chai School of Metallurgy and Environment Central South UniversityChangsha 410083China Chinese National Engineering Research Center for Control&Treatment of Heavy Metal Pollution Institute of Environmental EngineeringCentral South UniversityChangsha 410083HunanChin State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China Department of Building Civiland Environmental EngineeringConcordia UniversityMontreal H3G 1M8Canada

Arsenic materials have attracted great attention due to their unique ***,research concerning iron-arsenic(Fe-As) alloys is very scarce due to the volatility of As at low temperature and the high melting point of ***,a new Fe-As alloy was obtained by mechanical alloying(MA) followed by vacuum hot-pressed sintering(VHPS).Moreover,a systematic study was carried out on the microstructural evolution,phase composition,leaching toxicity of As,and physical and mechanical properties of Fe-As alloys with varying weight fractions of As(20%,25%,30%,35%,45%,55%,65%,and 75%).The results showed that pre-alloyed metallic powders(PAMPs) have a fine grain size and specific supersaturated solid solution after MA,which could effectively improve the mechanical properties of Fe-As alloys by VHPS.A high density(> 7.350 g·cm^(-3)),low toxicity,and excellent mechanical properties could be obtained for FeAs alloys sintered via VHPS by adding an appropriate amount of As,which is more valuable than commercial Fe-As *** Fe-25% As alloy with low toxicity and a relatively high density(7.635 g·cm^(-3)) provides an ultra-high compressive strength(1989.19 MPa),while the Fe-65% As alloy owns the maximum Vickers hardness(HVo.5 899.41).After leaching by the toxicity characteristic leaching procedure(TCLP),these alloys could still maintain good mechanical performance,and the strengthening mechanisms of Fe-As alloys before and after leaching were *** in the grain size,micro structure,and phase distribution induced significant differences in the compressive strength and hardness.

关键词： Mechanical alloying Vacuum hot-pressed sintering High-pressure Arsenic-iron alloys Mechanical properties

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Catalytic Reaction Kinetics of Propylene Dimerization to 4-Methyl-1-Pentene Using Cu-K/K_(2)CO_(3) Solid Base Catalyst

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China Petroleum Processing & Petrochemical Technology 2024年第1期26卷 78-87页

作者： Jin Haibo chai Jing Yang Suohe He Guangxiang Ma Lei Guo Xiaoyan College of New Materials and Chemical Engineering Beijing Institute of Petrochemical Technology/Beijing Key Laboratory of Fuel Cleaning and Efficient Catalytic Emission Reduction TechnologyBeijing102617China

The catalysis technology of propylene dimerization to form 4-methyl-1-pentene(4MP1)using a Cu-K/K_(2)CO_(3) solid base catalyst is a well-known heterogeneous catalytic *** this study,the intrinsic kinetics of propylene dimerization were studied in a fixed-bed continuous *** and external diffusion during the dimerization reaction experiments were eliminated by adjusting the flow rate of the carrier gas and the particle size of the catalyst ***,the concentration changes of each substance at the outlet of the catalyst bed under different residence times were ***,the suitable reaction kinetics equations was derived using the Langmuir Hinshelwood-Hougen-Watson kinetic ***,the activation energy for each reaction involved in the dimerization reaction was *** activation energies of 4MP1,branched by-products,and 1-hexene were 115.0,150.8,and 177.4 kJ/mol,*** effect of process conditions on propylene dimerization with solid base catalysts was studied through kinetic model *** comparing the theoretical values obtained from the simulation with the experimental results,the applicability and accuracy of the kinetic model were verified.

关键词： propylene dimerization 4-methyl-1-pentene intrinsic kinetics surface reaction

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Genomic insights into adaptation to bipedal saltation and desert-like habitats of jerboas

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Science China(Life Sciences) 2024年第9期67卷 2003-2015页

作者： Simin chai Yujie Chong Daiqing Yin Qiang Qiu Shixia Xu Guang Yang Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Jiangsu Key Laboratory for Biodiversity and Biotechnology School of Life SciencesNanjing Normal UniversityNanjing 210023China School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710072China

Jerboas is a lineage of small rodents displaying atypical mouse-like morphology with elongated strong hindlimbs and short *** have evolved obligate bipedal saltation and acute senses,and been well-adapted to vast desert-like *** a newly sequenced chromosome-scale genome of the Mongolian five-toed jerboa(Orientallactaga sibirica),our comparative genomic analyses and in vitro functional assays showed that the genetic innovations in both protein-coding and non-coding regions played an important role in jerboa morphological and physiological ***-specific amino acid substitutions,and segment insertions/deletions(indels)in conserved non-coding elements(CNEs)were found in components of proteoglycan biosynthesis pathway(XYLT1 and CHSY1),which plays an important role in limb ***,we found specific evolutionary changes functionally associated with energy or water metabolism(e.g.,specific amino acid substitutions in ND5 and indels in CNEs physically near ROR2)and senses(e.g.,expansion of vomeronasal receptors and the FAM136A gene family)in *** dual-luciferase reporter assay verified that some of the CNEs with jerboa-specific segment indels exerted a significantly different influence on luciferase activity,suggesting changes in their regulatory function in *** results revealed the potential molecular mechanisms underlying jerboa adaptation since the divergence from the Eocene-Oligocene transition,and provided more resources and new insights to enhance our understanding of the molecular basis underlying the phenotypic diversity and the environmental adaptation of mammals.

关键词： jerboa adaptive evolution comparative genomics conserved non-coding elements bipedal saltation

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Enhanced wide-angle third-harmonic generation in flat-band-engineered quasi-BIC metagratings

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 81-87页

作者： Yijia Zang Ruoheng chai Wenwei Liu Zhancheng Li Hua Cheng Jianguo Tian Shuqi Chen The Key Laboratory of Weak Light Nonlinear Photonics Ministry of EducationSchool of Physics and TEDA Institute of Applied PhysicsNankai UniversityTianjin 300071China School of Materials Science and Engineering Smart Sensing Interdisciplinary Science CenterNankai UniversityTianjin 300350China The Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Nonlinear metasurfaces and photonic crystals provide a significant way to generate and manipulate nonlinear signals owing to the resonance-and symmetry-based light-matter interactions supported by the artificial ***,the nonlinear conversion efficiency is generally limited by the angular dispersion of optical resonances especially in nonparaxial ***,we propose a metagrating realizing a quasi-bound-state in the continuum in a flat band to dramatically improve the third harmonic generation(THG)efficiency.A superior operating angular range is achieved based on the interlayer and intralayer couplings,which are introduced by breaking the mirror symmetry of the *** demonstrate the relation of angular dispersion between the nonlinear and linear responses at different incident *** also elucidate the mechanism of these offaxis flat-band-based nonlinear conversions through different mode *** scheme provides a robust and analytical way for nonparaxial nonlinear generation and paves the way for further applications such as wide-angle nonlinear information transmission and enhanced nonlinear generation under tight focusing.

关键词： nonlinear metasurfaces quasi-bound-state in the continuum angular dispersion third harmonic generation efficiency

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Robust, breathable, and chemical-resistant polytetrafluoroethylene (PTFE) films achieved by novel in-situ fibrillation strategy for highperformance triboelectric nanogenerators

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Nano Research 2024年第3期17卷 1942-1951页

作者： Jialong chai Guilong Wang Jinchuan Zhao Guizhen Wang Chao Wei Aimin Zhang Guoqun Zhao Laboratory for Liquid-Solid Structural Evolution and Processing of Materials(Ministry of Education) Shandong UniversityJinan 250061China Key Laboratory of Chinese Education Ministry for Tropical Biological Resources Hainan UniversityHaikou 570228China

In the era of advanced wearable electronic devices,the triboelectric nanogenerators(TENGs)as energy harvesting and self-powered sensing units hold great *** appropriate triboelectric material is the crucial factor to optimize the performance of TENG,while polytetrafluoroethylene(PTFE)stands out as a highly versatile option among the various *** this study,we present an ultrafine nanofibrous PTFE(NF-PTFE)films prepared by novel in-situ fibrillation strategy as the triboelectric material in TENG *** innovative processing methodology facilely addresses the dilemma between high porosity and fine pore size of traditional porous PTFE films,meanwhile achieves exceptional mechanical strength,hydrophobicity,air permeability,and chemical resistance of the *** the integration of nanofibrous PTFE films into contact-separation mode TENG and droplet-based TENG,these devices realize the peak electrical output of 131 V/10.8μA and 54 V/14μA with great durability,which surpass the performance of TENGs using traditional expanded PTFE ***,a smart glove capable of recognizing hand gestures is proposed,which demonstrates the versatility,flexibility,and practicality of this material for potential use in smart *** reported NF-PTFE film provides insights for the design of high-performance TENG device for advanced wearable electrical applications.

关键词： polytetrafluoroethylene(PTFE) nanofibrous film hydrophobic triboelectric nanogenerator self-powered sensor

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Development of Chinese herbal medicine for sensorineural hearing loss

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Acta Pharmaceutica Sinica B 2024年第2期14卷 455-467页

作者： Yunhao Wu Jingwen Zhang Qiuping Liu Zhuang Miao Renjie chai Wenyong Chen State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province HighTech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjing 210096China Department of Otolaryngology-Head and Neck the Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510120China Department of Otolaryngology-Head and Neck Surgery Affiliated Hospital of Jiangnan UniversityWuxi 214000China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610000China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 100085China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

According to the World Health Organization’s world report on hearing,nearly 2.5 billion people worldwide will suffer from hearing loss by 2050,which may contribute to a severe impact on individual life quality and national *** hearing loss(SNHL)occurs commonly as a result of noise exposure,aging,and ototoxic drugs,and is pathologically characterized by the impairment of mechanosensory hair cells of the inner ear,which is mainly triggered by reactive oxygen species accumulation,inflammation,and mitochondrial *** recent advances have been made in understanding the ability of cochlear repair and regeneration,there are still no effective therapeutic drugs for *** herbal medicine which is widely distributed and easily accessible in China has demonstrated a unique curative effect against SNHL with higher safety and lower cost compared with Western *** we present trends in research for Chinese herbal medicine for the treatment of SNHL,and elucidate their molecular mechanisms of action,to pave the way for further research and development of novel effective drugs in this field.

关键词： Sensorineural hearing loss Chinese herbal medicine Molecular mechanism Active ingredients Prescriptions Ototoxic drugs Noise Hair cells

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Silencing MFN2 Drives WNT/β-catenin Nucleation to Reduce Sorafenib Sensitivity in Hepatocellular Carcinoma Cells

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当代医学科学（英文） 2024年第4期44卷 789-798页

作者： chai-ming ZENG Bin SHAO Yan-ping CHEN Gui-sheng DING Department of Geriatric Medicine Shengli Clinical Medical College of Fujian Medical University Fujian Key Laboratory of Geriatrics DiseasesFujian Provincial Center for GeriatricsFujian Provincial HospitalFuzhou 350001China Department of Rehabilitation Shengli Clinical Medical College of Fujian Medical University Fujian Provincial Hospital Fuzhou 350001China Department of Gynecology Shengli Clinical Medical College of Fujian Medical University Fujian Provincial Hospital Fuzhou 350001China Department of Ultrasonography Shengli Clinical Medical College of Fujian Medical University Fujian Provincial Hospital Fuzhou 350001China

Objective:Mitofusin-2(MFN2)is a mitochondrial membrane protein that plays a critical role in regulating mitochondrial fusion and cellular *** further elucidate the impact of MFN2,this study aimed to investigate its significance on hepatocellular carcinoma(HCC)cell function and its potential role in mediating ***:This study investigated the effects of silencing and overexpressing MFN2 on the survival,proliferation,invasion and migration abilities,and sorafenib resistance of MHCC97-L HCC *** experiments were conducted using XAV939(a β-catenin inhibitor)and HLY78(a β-catenin activator)to further validate these ***:Silencing MFN2 significantly promoted the survival and proliferation of MHCC97-L cells,enhanced their invasion and migration capacities,increased the IC50 of sorafenib,reduced the percentage of TUNEL-positive cells,and decreased the expression of proapoptotic ***,silencing MFN2 markedly induced the nuclear translocation of β-catenin,increased β-catenin acetylation levels and enhanced the expression of the downstream regulatory proteins Snail 1 and Vimentin while inhibiting E-cadherin ***,overexpressing MFN2 reversed the effects observed in MHCC97-L cells mentioned *** results confirmed that silencing MFN2 activated the β-catenin/epithelial-mesenchymal transition(EMT)pathway and reduced the sensitivity of cells to sorafenib,which could be reversed by XAV939 ***,overexpression of MFN2 inhibited the β-catenin/EMT pathway and increased the sensitivity of cells to sorafenib,which could be altered by ***:Low expression of MFN2 in HCC cells promotes the nuclear translocation of β-catenin,thereby activating the EMT pathway and mediating resistance to sorafenib.

关键词： apoptosis epithelial-mesenchymal transition hepatocellular carcinoma mitofusin-2 sorafenib resistance

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