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Effects of proteins on magnesium degradation-static vs.dynamic conditions

Journal of Magnesium and Alloys 2023年第4期11卷 1332-1342页

作者： Ruiqing Hou Frank Feyerabend Heike Helmholz Vasil M.Garamus Regine Willumeit-Römer School of Materials Science and Engineering&Henan Key Laboratory of Advanced Magnesium Alloys Zhengzhou UniversityNo.100 Science AvenueZhengzhou 450001China Institute of Metallic Biomaterials Helmholtz-Zentrum Hereon(Hereon)Max-Planck-Str.1Geesthacht 21502Germany

The interaction between organic molecules and biomaterial surfaces determines the fate of biomaterials during their service life,which is also the research hotspots in the field of biomaterials.To understand the mechanism of protein interaction with magnesium(Mg)degradation,alloying elements,immersion time,protein concentration and surface conditions have been previously considered for the effect of proteins on Mg degradation.However,fluid flow,as one of the critical factors,drew little attention in this case.In the present study,the effect of bovine serum albumin(BSA)and fetal bovine serum(FBS)on Mg degradation was compared under static and dynamic conditions.The results revealed that both BSA and FBS slightly decreased the degradation rate of Mg in Hanks’balanced salt solution(HBSS)under static immersion due to the protein adsorption and the formation of a Ca/P-rich top layer on Mg surface,whereas under dynamic flow condition the degradation of Mg was significantly accelerated in the presence of BSA or FBS.The reasons seemed to stem from the weakened protein adsorption on Mg surface in this case and the dynamically enhanced interaction between proteins and ions/products in solutions,which largely weaken the combination of the top Ca/P-rich layer with the inner corrosion product layer.These results highlight the importance of testing conditions for Mg characterization in vitro and the synergistic effect between different parameters on Mg degradation.

关键词： Flow Bovine serum albumin Fetal bovine serum Protein adsorption

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First Isolation and Characterization of Ochrobactrum anthropi from Pig

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Engineering 2020年第1期6卷 49-55页

作者： Shijiang Gu Ruiqing Hou Shengguo Gao Zhe Sun Xiangdong Li Lufeng Zhai Yunyun Jin Qiaoyan Zhu Yonghong Liao Kegong Tian National Research Center for Veterinary Medicine Luoyang 471000China College of Animal Science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002China

One Gram-negative Bacillus was isolated from a brain sample of a pig with neurological symptoms.Pathological examination showed meningitis at necropsy. Ochrobactrum anthropi (O. anthropi) was successfully isolated from the brain sample and was confirmed by biochemical reaction results (API 20 NE) and gene sequencing. The strain was highly resistant to b-lactam antibiotics. Mice were experimentally infected with O. anthropi and showed typical meningitis. This is the first report on O. anthropi isolated from a pig, and indicates that O. anthropi may have a broader host spectrum of infection.

关键词： Ochrobactrum anthropi Pig brain

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无缝隙导向型护理模式在腹腔镜下胆总管切开取石术患者中的应用

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保健医学研究与实践 2023年第2期20卷 120-123页

作者：侯瑞青河南省洛阳市第一人民医院急诊科河南洛阳471003

目的探讨无缝隙导向型护理模式在腹腔镜下胆总管切开取石术患者中的应用效果,以期为临床治疗提供参考。方法本研究选取2020年4月—2022年6月于河南省洛阳市第一人民医院就诊的胆总管结石患者136例为研究对象。采用随机数字表法,将患者... 详细信息

目的探讨无缝隙导向型护理模式在腹腔镜下胆总管切开取石术患者中的应用效果,以期为临床治疗提供参考。方法本研究选取2020年4月—2022年6月于河南省洛阳市第一人民医院就诊的胆总管结石患者136例为研究对象。采用随机数字表法,将患者分为研究组(n=68)和常规组(n=68)。常规组患者给予常规护理干预,研究组患者在常规组基础上进行无缝隙导向型护理干预。比较2组患者术后排气、排便、下床活动、进食、住院时间等康复指标。比较2组患者入院时(干预前)、麻醉前、出院当天(干预后)焦虑自评量表(SAS)、抑郁自评量表(SDS)评分。比较2组患者干预前后视觉模拟量表(VAS)评分。比较2组患者干预期间并发症发生情况。结果研究组患者排气、进食、下床活动、排便、住院时间均短于常规组,差异均有统计学意义(P0.05)。干预后,2组患者SAS、SDS评分均低于干预前,且研究组低于常规组,差异均有统计学意义(P0.05)。干预后,2组患者VAS评分均低于干预前,且研究组低于常规组,差异均有统计学意义(P<0.05)。研究组患者并发症总发生率为0%,低于常规组的8.82%,差异有统计学意义(P<0.05)。结论无缝隙导向型护理模式可改善腹腔镜下胆总管切开取石术患者的负性情绪,减轻患者疼痛感,降低并发症发生率,促进患者病情恢复,值得推广应用。

关键词：无缝隙导向型护理模式腹腔镜胆总管结石

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阴道后壁网片修补术用于阴道后壁膨出伴便秘的临床研究

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结直肠肛门外科 2016年第2期22卷 178-181页

作者：侯瑞青河北省滦平县滦平中医院妇科河北滦平068250

目的探讨阴道后壁网片修补术在治疗阴道后壁膨出伴便秘的临床疗效。方法回顾性分析我院2015年5月至2015年10月采用阴道后壁网片修补术治疗阴道后壁膨出的50例患者，查找记录患者的病情资料，手术记录以及术后并发症及随访情况。对阴道... 详细信息

目的探讨阴道后壁网片修补术在治疗阴道后壁膨出伴便秘的临床疗效。方法回顾性分析我院2015年5月至2015年10月采用阴道后壁网片修补术治疗阴道后壁膨出的50例患者，查找记录患者的病情资料，手术记录以及术后并发症及随访情况。对阴道后壁网片修补术对于治疗阴道后壁膨出伴便秘的安全性和有效性进行分析。结果本研究中50例患者均成功完成手术，手术时间为（37．2±3．3）min，术中出血量为（20．5±4．1）mL，住院时间为（4．7±0．8）d。术后患者均无阴道前壁膨出，无出血和尿潴留现象，2例患者直肠指诊检查显示直肠前突处紧张，但未向阴道突出；5例患者出现肛门会阴处疼痛，1—2周消失。随访6-18个月结果显示，未发现患者出现并发症和复发现象。结论阴道后壁网片修补术治疗阴道后壁膨出伴便秘具有良好的效果，创伤小，恢复快，并发症和复发率少，值得进一步推广。

关键词：阴道后壁膨出网片修补术:手术

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春玉米温度适宜度动态空间化方法研究

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中国农业资源与区划 2017年第1期38卷 45-50页

作者：张超侯琼郭瑞清内蒙古生态与农业气象中心呼和浩特010051

为了利用新数据、新方法提高温度适宜度指标在河套地区空间化精度,该研究利用区域站数据开展玉米温度适宜度动态空间化方法研究。首先对区域站逐小时气温数据进行质量控制,计算日平均气温并采用全局多项式插值法进行空间分布拟合;绘制... 详细信息

为了利用新数据、新方法提高温度适宜度指标在河套地区空间化精度,该研究利用区域站数据开展玉米温度适宜度动态空间化方法研究。首先对区域站逐小时气温数据进行质量控制,计算日平均气温并采用全局多项式插值法进行空间分布拟合;绘制河套地区玉米播种普期空间分布图,根据播种后日尺度的温度适宜度模型,利用GIS栅格计算功能获得最适温度、最高温度、最低温度等指标的空间分布;建立空间分析计算流程,利用GIS条件函数构造日尺度空间温度适宜度分段函数,实现逐日温度适宜度指标的空间化计算;采用常规气象站进行误差分析。结果表明:(1)日平均气温拟合结果与站点观测值温差0.69℃,平均相对误差3.91%,区域站点密集,日平均气温的全局多项式插值效果较好;(2)温度适宜度平均绝对误差0.08,平均相对误差0.41%;(3)利用Model Builder工具建立的播种后第i日温度适宜度指标自动处理流程,实现了复杂模型与数据的综合快速应用。综上,区域站数据在实现玉米温度适宜度动态空间化方面具有较高的业务应用价值。

关键词：河套地区温度适宜度区域站条件函数

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Autophagy receptor CCDC50 tunes the STING-mediated interferon response in viral infections and autoimmune diseases

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Cellular & Molecular Immunology 2021年第10期18卷 2358-2371页

作者： Panpan hou Yuxin Lin Zibo Li ruiqing Lu Yicheng Wang Tian Tian Penghui Jia Xi Zhang Liu Cao Zhongwei Zhou Chunmei Li Jieruo Gu Deyin Guo MOE Key Laboratory of Tropical Disease Control Centre for Infection and Immunity Study(CIIS)School of MedicineSun Yat-sen UniversityShenzhenChina The Center for Applied Genomics Abramson Research CenterThe Children's Hospital of PhiladelphiaPhiladelphiaPAUSA Division of Rheumatology Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina

DNA sensing and timely activation of interferon(IFN)-mediated innate immunity are crucial for the defense against DNA virus infections and the clearance of abnormal cells.However,overactivation of immune responses may lead to tissue damage and autoimmune diseases;therefore,these processes must be intricately regulated.STING is the key adaptor protein,which is activated by cyclic GMP-AMP,the second messenger derived from cGAS-mediated DNA sensing.Here,we report that CCDC50,a newly identified autophagy receptor,tunes STING-directed type I IFN signaling activity by delivering K63-polyubiquitinated STING to autolysosomes for degradation.Knockout of CCDC50 significantly increases herpes simplex virus 1(HSV-1)-or DNA ligand-induced production of type I IFN and proinflammatory cytokines.Ccdc50-deficient mice show increased production of IFN,decreased viral replication,reduced cell infiltration,and improved survival rates compared with their wild-type littermates when challenged with HSV-1.Remarkably,the expression of CCDC50 is downregulated in systemic lupus erythematosus(SLE),a chronic autoimmune disease.CCDC50 levels are negatively correlated with IFN signaling pathway activation and disease severity in human SLE patients.CCDC50 deficiency potentiates the cGAS-STING-mediated immune response triggered by SLE serum.Thus,our findings reveal the critical role of CCDC50 in the immune regulation of viral infections and autoimmune diseases and provide insights into the therapeutic implications of CCDC50 manipulation.

关键词： CCDC50 STING Type I IFN HSV-1 Autophagy Autoimmune diseases

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Enhancement of a recombinase-aided amplification assay using betaine and pullulan

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Infectious Medicine 2022年第2期1卷 73-80页

作者： Jinrong Wang Guowei Song Yue Ming Jing Pan ruiqing Zhang Guohao Fan Xinxin Shen Xuejun Ma Lixin Li Shijiazhuang People’s Hospital ShijiazhuangHebei050051China Qiqihar Medical University QiqiharHeilongjiang161000China NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China

Background:Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification(RAA)assay were studied for the first time,and amplification of a long-fragment(509 bp)was initially explored.Methods:Using recombinant plasmids and clinical samples,RAA fluorescence and basic methods were used to evaluate the efficacy.The fluorescence method was evaluated by threshold time and fluorescence value,and the basic method was characterized by 2%agarose gel electrophoresis.Results:Taking a previously established RAA assay for HPV18 as an example,we demonstrated that the addition of 0.2 M,0.4 M,and 0.6 M betaine and 10%pullulan could enhance the RAA.The new RAA assays with betaine and pullulan were named B-RAA and P-RAA,respectively.Using the B-RAA and P-RAA fluorescence methods,the threshold time values could be shortened by 1.72-2.32 minutes and 2.60 minutes,respectively,and the fluorescence values could be enhanced by 8847.25-9094.37 mv and 5250 mv,respectively.Using the basic method,the sensitivity could be increased 10-fold.We successfully amplified a long-fragment of 509 bp using a P-RAA assay with a sensitivity of 102 copies/μL(compared with 103 copies/μL in the RAA assay).Conclusions:Thus,we concluded that betaine and pullulan are effective additives to enhance the sensitivity of RAA assays.

关键词： Nucleic acid amplification enhancer Betaine Pullulan RAA Long-fragment

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肿瘤坏死因子α短期刺激促进骨髓间充质干细胞的成骨分化

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东南大学学报（医学版） 2021年第4期40卷 500-505页

作者：侯立鹏马丽芳王瑞青西安市第九医院口腔科陕西西安710054 军事口腔医学国家重点实验室国家口腔疾病临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院牙体牙髓病科陕西西安710032 第四军医大学基础医学院陕西西安710032

目的:探究肿瘤坏死因子α(TNF-α)刺激3 d对骨髓间充质干细胞(BMMSCs)增殖和成骨分化的影响。方法:不同浓度(0、1、10、50 ng·ml^(-1))的TNF-α刺激BMMSCs 3 d后,MTS细胞增殖检测试剂盒检测BMMSCs继续培养1、4、7 d后的体外增殖活性;... 详细信息

目的:探究肿瘤坏死因子α(TNF-α)刺激3 d对骨髓间充质干细胞(BMMSCs)增殖和成骨分化的影响。方法:不同浓度(0、1、10、50 ng·ml^(-1))的TNF-α刺激BMMSCs 3 d后,MTS细胞增殖检测试剂盒检测BMMSCs继续培养1、4、7 d后的体外增殖活性;细胞迁移实验检测其体外迁移能力;成骨诱导液继续诱导2周后检测其体外碱性磷酸酶(ALP)活性;水凝胶分别包裹1、50 ng·ml^(-1)的TNF-α在小鼠极限模型中植入,8周后取材通过微计算机断层扫描技术(micro-CT)和HE染色检测新骨形成能力。结果:与对照组比较,TNF-α处理组刺激3 d对细胞增殖无影响,但可显著促进BMMSCs的迁移,其中50 ng·ml^(-1)浓度迁移效果最好。ALP活性检测显示,在2周时1、10、50 ng·ml^(-1)TNF-α均能显著促进BMMSCs的迁移(P<0.05),并呈浓度依赖性递增,50 ng·ml^(-1)的TNF-α效果最好。成功构建了水凝胶TNF-α短期缓释系统,小鼠体内micro-CT和HE染色显示50 ng·ml^(-1)TNF-α短期缓释组成骨效果最好,能有效促进新骨形成。结论:本实验证实TNF-α成骨诱导液短时间刺激可有效促进BMMSCs在体内体外的成骨分化。

关键词：肿瘤坏死因子α 水凝胶骨髓间充质干细胞成骨分化

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Brucellosis Knowledge and Personal Protective Equipment Usage Among High-Risk Populations in Brucellosis-Endemic Areas-China,2019−2020

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China CDC weekly 2021年第6期3卷 106-109页

作者： Zhe Wang Shenghong Lin Xinrong Liu Aizhi Yu Muhtar·Hasan Jiensi·Bayidawulieti Haitan·Aximujiang ruiqing Li Guoxing Zheng Xinwang Liang Xiaoling Fan Biqiao hou Xiaolong Fan Dilxat·Abuliti Lusha Shi Cuihong Zhang Yifei Wang Pengjing Ning Caixiong Liu Zhongjie Li Liping Wang Hangzhou Center for Disease Control and Prevention HangzhouZhejiangChina Division of Infectious Disease Key Laboratory of Surveillance and Early Warning on Infectious DiseaseChinese Center for Disease Control and PreventionBeijing China Datong Center for Disease Control and Prevention DatongShanxiChina Huocheng Center for Disease Control and Prevention YiliXinjiangChina Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention UrumqiXinjiang Uygur Autonomous RegionChina Yili Kazakh Autonomous Prefecture Center for Disease Control and Prevention YiliXinjiangChina Yanggao Center for Disease Control and Prevention DatongShanxiChina Zuoyun Center for Disease Control and Prevention DatongShanxiChina Hunyuan Center for Disease Control and Prevention DatongShanxiChina

What is already known about this topic?According to the National Brucellosis Prevention and Control Plan(2016–2020)(NBPCP),the awareness rate of high-risk populations in brucellosis-endemic areas should reach 90%by 2020.But the updated results have not been reported.What is added by this report?This report determined the awareness rate of brucellosis(17.74%),utilization of personal protective equipment(PPE)(20.13%),and their relationship with seroprevalence,which provides evidence for the effectiveness of the implementation of NBPCP.What are the implications for public health practice?The results suggest that health education should be conducted for high-risk populations to improve their brucellosis and protection knowledge.

关键词： Population Brucellosis

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Serological Prevalence Survey Among the High-Risk Populations of Brucellosis-Endemic Areas-China,2019−2020

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China CDC weekly 2021年第6期3卷 101-105页

作者： Shenghong Lin Zhe Wang Xinrong Liu Aizhi Yu Muhtar·Hasan Jiensi·Bayidawulieti Haitan·Aximujiang ruiqing Li Guoxing Zheng Xinwang Liang Xiaoling Fan Biqiao hou Xiaolong Fan Dilxat·Abuliti Lusha Shi Cuihong Zhang Yifei Wang Pengjing Ning Caixiong Liu Zhongjie Li Liping Wang Division of Infectious Disease Key Laboratory of Surveillance and Early Warning on Infectious DiseaseChina Center for Disease Control and PreventionBeijingChina Hangzhou Center for Disease Control and Prevention HangzhouZhejiangChina Datong Center for Disease Control and Prevention DatongShanxiChina Huocheng Center for Disease Control and Prevention YiliXinjiangChina Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention UrumqiXinjiangChina Yili Kazakh Autonomous Prefecture Center for Disease Control and Prevention YiliXinjiangChina Yanggao Center for Disease Control and Prevention DatongShanxiChina Zuoyun Center for Disease Control and Prevention DatongShanxiChina Hunyuan Center for Disease Control and Prevention DatongShanxiChina

What is already known about this topic?Timely screening of high-risk population is important to improve the early detection of brucellosis among the endemic areas during the high incidence seasons,which is also required by the National Brucellosis Prevention and Control Plan(2016–2020)(NBPCP).What is added by this report?Seroepidemiological characteristics of brucellosis in high-risk populations were obtained and special occupational populations were found.The seroprevalence of brucellosis has been decreasing compared with that reported in the recent years due to the ongoing implementation of control measures in endemic areas.What are the implications for public health practice?Special occupational populations could be promptly detected using routine screening,which makes it possible to initiate standardized treatment for infected patients as early as possible.It also reminds us to pay attention to special occupational populations to improve their knowledge of brucellosis and reduce the risk of infection.

关键词： Population Brucellosis incidence

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