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Science China Chemistry 2017年第11期60卷 1468-1473页

作者： Manjun Zhang ruimin li Jing Wang liansheng ling School of Chemistry Sun Yat-Sen UniversityGuangzhou 510275China

Nucleic acid amplification test is a reliable method for primary human immunodeficiency virus （HIV） infection diagnosis. Herein, a novel fluorescent method for sequence-specific recognition of DNA fragment of HIV-1 was established based upon nicking-assisted strand displacement amplification （SDA） and triplex DNA. In the presence of target dsDNA, nicking-assisted SDA process generated a lot of ssDNA, which hybridized with molecular beacon to produce signal. The fluorescence intensity was proportional to the concentration of target dsDNA within the range from 5 to 1000 pmol/L, with a detection limit of 1.4 pmol/L. Moreover, it successfully distinguished target dsDNA from the nucleic acid extractive of human blood. Thus this method has the merit of high sensitivity, and it is suitable for sequence-specific recognition of target dsDNA in complex matrices, which made it a potential application in diagnosis of acquired immunodeflciency syndrome （AIDS） in the future.

关键词： HIV-1 dsDNA nicking-assisted strand displacement amplification triplex DNA Y-shaped structure fluorescence

Traffic control optimization strategy based on license plate recognition data

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Journal of Traffic and Transportation Engineering(English Edition) 2023年第1期10卷 45-57页

作者： ruimin li Shi Wang Pengpeng Jiao Shichao lin Institute of Transportation Engineering Tsinghua UniversityBeijing 100084China Department of Civil Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA School of Civil and Transportation Engineering Beijing University of Civil Engineering and ArchitectureBeijing 100044China

Traffic signal control is essential to the efficiency of the road network’s *** recent years,more and more detailed detection data provide potential data support for traffic signal control,such as license plate recognition(LPR)*** study aims to develop a traffic signal control optimization method based on model predictive control(MPC)and LPR *** proposed framework of a closed-loop control system is described in ***,the control objectives and queue prediction model for signalized intersection are ***,online optimization and feedback compensation are discussed and *** of the arrival rate at the downstream are based on the LPR data detected at the upstream intersection,and dynamic optimization method of the offset is proposed for a coordinated *** model is validated using the LPR data of two consecutive intersections with a traffic simulation *** demonstrate that the model can restrain extreme long queuing,improve intersection capacity,and reduce intersection average *** developed model promotes the system operating efficiency and shows the general advantage of real-time optimization,feedback,and *** proposed framework can be potentially applied by local traffic management centers to improve the quality of traffic signal control.

关键词： Traffic control Model predictive control Closed-loop control license plate recognition data

An Ag＋-stabilized triplex DNA molecular switch controlled hybridization chain reaction

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Science China Chemistry 2017年第12期60卷 1575-1580页

作者： Yanwei Luo ruimin li Jing Wang Manjun Zhang li Zou liansheng ling School of Chemistry Sun Yat-Sen UniversityGuangzhou 510275China

We described a new strategy to combine hybridization chain reaction(HCR) process and triplex DNA through Ag+/cysteine,thus to pursue the controllable process of HCR. H1, a specially-designed triplex DNA, with homopyrimidines which can bind to dsDNA through Hoogsteen bonding, forming a cap-like triplex DNA. The presence of Ag^+ can play the role as a H1 locker to stop initiator from triggering HCR process, forming Ag+-stabilized triplex DNA molecular switch(denoted as Ag^+-STDMS). However,the strong binding ability of cysteine towards Ag^+ forming stable Ag^+-cysteine can release the locking tail of H1 and realize the retriggering of HCR. This study presented a promising tool to regulate the self-assembly processes of DNA-based nanostructures in neutral environment. Under the optimum conditions, fluorescence intensity(peaking at 582 nm) of HCR is proportional to the concentration of Ag+ in the 0.2–4.0 μM range. Within the presence of Ag^+, the fluorescence intensity is also proportional to the concentration of cysteine in the 0.2–3.0 μM range. The method can successfully manipulate the assembly and disassembly of DNA in HCR.

关键词： triplex DNA HCR self-assembly Ag^+ cysteine fluorescence

Driver injury severity analysis of crashes in a western China’s rural mountainous county:Taking crash compatibility difference into consideration

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Journal of Traffic and Transportation Engineering(English Edition) 2021年第5期8卷 703-714页

作者： liyang Wang ruimin li Changjun Wang Zhiyong liu Key Laboratory of the Ministry of Public Security for Road Traffic Safety Ministry of Public SecurityWuxi 214151China Traffic Management Research Institute of the Ministry of Public Security Wuxi 214151China Institute of Transportation Engineering Department of Civil EngineeringTsinghua UniversityBeijing 100084China

China’s traffic safety attracts increasing research *** data show that crashes in the western region of China are more severe than those in the eastern ***,research on crash severity in western China is *** study applied a hierarchical Bayesian logistic model to examine the significant factors related to crash and vehicle/driver levels and their heterogeneous impacts on the severity of drivers’ *** data were collected from lintao,a rural mountainous county in western China.A variable was proposed to measure the relative difference between the crashworthiness of one vehicle and the aggressivity of the other vehicle in the mixed traffic *** indicated that the majority of the total variance was induced by between-crash variance,showing the suitability of the utilized hierarchical modeling *** crash-level variable and six vehicle/driver-level variables,namely,road type,compatibility difference,age,vehicle type,drunk driving,driving unregistered vehicle,and driving years,significantly affected modeling drivers’ injury *** these variables,road type(national and provincial),age(young and senior drivers),driving unregistered vehicle,and drunk driving tended to increase the odds of crash-related *** years(new drivers with less than six years of driving experience) and vehicle type(heavy vehicle) were likely to decrease the probability of fatal *** difference was relatively significant,and the possibilities of mortality in single vehicle crashes were higher than those inmultivehicle and pedestrian-involved *** developed methodology and estimation results provided insights into the internal mechanism of rural crashes and effective countermeasures to prevent rural crashes.

关键词： Transportation engineering Driver injury severity Western China rural mountainous county Traffic crash Hierarchical model Bayesian inference

外源水杨酸、褪黑素对盐胁迫下大豆种子萌发和幼苗生长的影响

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安徽农学通报 2024年第19期30卷 31-36页

作者：李傲宇李瑞敏陈翼芝于爽牡丹江师范学院生命科学与技术学院黑龙江牡丹江157011

为研究外源水杨酸、褪黑素对盐胁迫下大豆种子萌发和幼苗生长的影响,筛选出效果较为明显的缓解剂。本试验以大豆吉育204为材料,对100 mmol/L NaCl胁迫下的大豆种子分别外源施加浓度为0.1、0.2和0.5 mmol/L的水杨酸或褪黑素,进行培养皿... 详细信息

为研究外源水杨酸、褪黑素对盐胁迫下大豆种子萌发和幼苗生长的影响,筛选出效果较为明显的缓解剂。本试验以大豆吉育204为材料,对100 mmol/L NaCl胁迫下的大豆种子分别外源施加浓度为0.1、0.2和0.5 mmol/L的水杨酸或褪黑素,进行培养皿纸上发芽试验,对其发芽率、发芽势、发芽指数、活力指数、相对盐害率以及幼苗鲜重和苗长进行测定。结果表明,与CK0(空白对照、未经NaCl胁迫)相比,100 mmol/L NaCl胁迫下的大豆种子发芽率、发芽势、发芽指数、活力指数和幼苗鲜重、苗长分别下降了28.34%、21.67%、35.89%、47.79%、21.16%和47.82%,相对盐害率30.91%。0.1、0.2和0.5 mmol/L的水杨酸或褪黑素对盐胁迫下大豆种子发芽率、发芽势、发芽指数、活力指数和幼苗鲜重、苗长均有促进作用,并降低了相对盐害率。其中,与CK1(NaCl胁迫)相比较,0.2 mmol/L褪黑素处理的盐胁迫大豆种子的发芽率、发芽势、发芽指数、活力指数、幼苗鲜重和苗长分别提高23.00%、25.00%、43.43%、66.84%、20.19%和19.12%,并使相对盐害率降至23.48%。褪黑素对大豆盐胁迫的缓解效应整体上优于水杨酸,可作为大豆抵御盐胁迫的较优缓解剂。

关键词：盐胁迫水杨酸褪黑素大豆种子萌发幼苗生长

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考虑排放控制区的多目标船舶航速优化研究

舰船科学技术 2024年第17期46卷 20-26页

作者：郭霆黄忠政李瑞民吉承成赖祥华江苏科技大学能源与动力学院江苏镇江202212 海丰国际控股有限公司上海200120 江苏远望仪器集团有限公司江苏镇江202212 扬帆集团股份有限公司浙江舟山316100

考虑在船舶排放控制区背景下,以燃油成本和租船成本为优化目标进行多目标船舶航速优化,选择基于蝴蝶优化算法与线性加权相结合和多目标蜻蜓算法的2种多目标优化方法,求解得出Pareto最优解集,最后通过理想点距离法的决策方法从Pareto最... 详细信息

考虑在船舶排放控制区背景下,以燃油成本和租船成本为优化目标进行多目标船舶航速优化,选择基于蝴蝶优化算法与线性加权相结合和多目标蜻蜓算法的2种多目标优化方法,求解得出Pareto最优解集,最后通过理想点距离法的决策方法从Pareto最优解集中确定出最佳折衷解。结果表明,当以多目标蜻蜓算法优化航速航行时,燃油成本降低2.72%,租船成本降低15.57%,可有效降低5.78%的船舶营运成本,更能大大提高船东和航运企业的经济效益,更符合环保要求。

关键词：多目标船舶航速优化优化算法

Hairpin Probe for Sequence-specific Recognition of Double-stranded DNA on Simian Virus 40

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Chemical Research in Chinese Universities 2018年第1期34卷 28-32页

作者： ZHANG Hong ZOU li li ruimin ZHAO Mingqin liNG liansheng School of Chemistry Sun Yat-sen University Guangzhou 510275 R R. China College of Tobacco Science Henan Agricultural University Zhengzhou 450000 P. R. China

Simian virus 40（SV40） is a polyomavirus and can induce a series of different tumors. The recognition of SV40 genome is crucial to tumor diagnosis and gene therapy. Herein, a sensitive and selective colorimetric method for sequence-specific recognition of homopyrimidine-homopurine duplex DNA（dsDNA） of SV40（4424-4440, gp6） was established with a hairpin probe based upon the formation of triplex DNA. Hairpin probe 5＇-CCC TAC CCA TTT TTT CTT CTC TTT CCT GGG TAG GGC GGG TTG GG-3＇（HP） containing G-rich sequence and 17-bp triplex-forming sequence was used as the signal probe, which was stem-loop structure alone and exhibited low catalytic activity. Upon its binding to the target duplex of SV40, hairpin probe transferred from stem-loop structttre to parallel triplex DNA, accompanied by the recovery of catalytic activity of DNAzyme and a sharp increase of absorbance. Under optimum conditions, the absorbance was increased proportionally to the concentration of dsDNA over the range from 500 pmol/L to 40.0 nmol/L with a detection limit of 433 pmol/L. Moreover, satisfied results were obtained when the assay was used to recognize the mismatched sequences.

关键词： Simian virus 40 Hairpin probe Triplex DNA G-Quadruplex DNAzyme

Effects of odd-even traffic restriction on travel speed and traffic volume:Evidence from Beijing Olympic Games

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Journal of Traffic and Transportation Engineering(English Edition) 2016年第1期3卷 71-81页

作者： ruimin li Min Guo Institute of Transportation Engineering Department of Civil EngineeringTsinghua UniversityBeijing 100084China Beijing Traffic Management Bureau Beijing 100037China

This paper reports the effects of using an ＂odd and even＂ traffic restriction policy in Beijing during the 2008 Olympic Games. Based on data from 529 traffic detectors on the expressway network and some main arterials in Beijing, China, a comparative analysis has been carried out on the following parameters： the total traffic volume within the expressway network, the total traffic volume on different ring expressways, the traffic volume and speed of a freeway segment, and an arterial street before and after the implementation of the traffic restriction policy. The results show that during the traffic restriction period, although more than 50% of vehicles were forbidden to travel in Beijing, the traffic volume was only reduced by 20%-40% while the travel speed had been increased by 10%-20%. This suggests that such traffic restriction policy may be an effective shortterm management measure in dealing with increased transportation demand and congestion during major events, such as the Olympic Games. Results also indicate that vehicle travel demand does not decrease with the same proportion as the total vehicles forbidden, at least for the expressway and main arterials in a city.

关键词： Traffic demand managementTraffic restriction policyComparative analysisTraffic detection system

Influencing factors analysis of helmet wearing for electric bicycle riders based on ordinal multinomial logistic model

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Transportation Safety and Environment 2022年第1期4卷 63-75页

作者： Quan Yuan Haixu Shi Junwei Zhao ruimin li State Key Laboratory of Automotive Safety and Energy School of Vehicle and MobilityTsinghua UniversityBeijing 100084China School of Transportation Science and Engineering Beihang UniversityBeijing 102206China Institute of Transportation Engineering Department of Civil EngineeringTsinghua UniversityBeijing 100084China

The helmet of riders of electric bicycles plays an important role in reducing injuries and deaths in traffic *** paper conducts a questionnaire survey,data analysis and modelling to investigate the influencing factors of electric bicycle helmet ***,living area,gender,age,marital status and education level are selected as independent variables for data *** factor analysis divides the sentiments of electric bicyclists for wearing helmets into four aspects:safety perception,practical sensation,satisfaction perception and emergency perception,and ordinal multiple logistic models are built to analyse the influencing *** result shows that people aged 18−25,26−35,36−45 and 46−55 are 1.3,1.8,2.0 and 2.3 times more likely,respectively,to have at least a grade higher safety perception than those aged 56 and over;men are 0.77 times more likely than women to feel at least one grade higher about the practical perception and 1.48 times more than women about the satisfaction perception;people with primary school,junior high school,senior high school,junior college and bachelor’s degree education are 1.64,2.44,1.50,1.70 and 1.55 times more likely,respectively,than people with a master’s degree to feel at least one grade higher about the satisfaction perception.

关键词： traffic safety electric bicycle helmet logistic model influencing factors

改进的经验模态分解方法和解析能量算子在电机轴承故障诊断中的应用

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计算机测量与控制 2024年第8期32卷 72-77,85页

作者：姚丹孙敏李睿敏付锐南江萍西安交通工程学院机械与电气工程学院西安710300

针对电机轴承故障信号能量微弱导致极易被噪声干扰所淹没的不足,进行了电机轴承故障特征提取研究;提出了一种改进的经验模态分解方法,包括谱峭度指标和一种新颖的解析能量算子相结合的电机轴承故障诊断方法;经实际测试与应用实现了对电... 详细信息

针对电机轴承故障信号能量微弱导致极易被噪声干扰所淹没的不足,进行了电机轴承故障特征提取研究;提出了一种改进的经验模态分解方法,包括谱峭度指标和一种新颖的解析能量算子相结合的电机轴承故障诊断方法;经实际测试与应用实现了对电机轴承故障信号中背景噪声干扰的去除,从而满足了电机轴承微弱故障特征提取的应用;通过与常用的电机轴承故障诊断方法对比验证了所提方法的有效性和优越性,为电机轴承故障诊断提供一个新的思路。

关键词：电机轴承故障诊断改进的经验模态分解方法包络谱峭度解析能量算子

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