检索结果-南通市图书馆

Oncology Research 2024年第4期32卷 643-658页

作者： LEI LIU NA GUO XIANGLING LI QIAN XU ruilong he LIMIN CheNG CHUNYAN DANG XINYU BAI YIYING BAI XIN WANG QIANHUI CheN LI ZHANG Department of Immunology Chengde Medical UniversityChengde067000China Department of Pathology Chengde Medical UniversityChengde067000China Department of Oncology The Affiliated Hospital of Chengde Medical UniversityChengde067000China Department of hepatobiliary Surgery The Affiliated Hospital of Chengde Medical UniversityChengde067000China

The platinum-based chemotherapy is one of the most frequently used treatment protocols for lung adenocarcinoma(LUAD),and chemoresistance,however,usually results in treatment failure and limits its application in the *** has been shown that microRNAs(miRNAs)play a significant role in tumor *** this study,miR-125b was identified as a specific cisplatin(DDP)-resistant gene in LUAD,as indicated by the bioinformatics analysis and the real-time quantitative PCR *** decreased serum level of miR-125b in LUAD patients was correlated with the poor treatment response rate and short survival ***-125b decreased the A549/DDP proliferation,and the multiple drug resistance-and autophagy-related protein expression levels,which were all reversed by the inhibition of *** addition,xenografts of human tumors in nude mice were suppressed by miR-125b,demonstrating that through autophagy regulation,miR-125b could reverse the DDP resistance in LUAD cells,both in vitro and in *** mechanistic studies indicated that miR-125b directly repressed the expression levels of RORA and its downstream BNIP3L,which in turn inhibited autophagy and reversed *** on these findings,miR-125b in combination with DDP might be an effective treatment option to overcome DDP resistance in LUAD.

关键词： Lung adenocarcinoma miRNAs Cisplatin Resistance Autophagy

重组人白细胞介素-1受体拮抗剂对眼角膜上皮细胞损伤的修复作用研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国实用医刊 2016年第17期43卷 93-95页

作者：贺瑞龙山西省临县人民医院眼科 033200

目的：研究重组人白细胞介素-1受体拮抗剂（RhIL-1ra）对眼角膜上皮细胞损伤的修复作用。方法将40只大鼠随机分为五组：对照组、模型组和 RhIL-1ra（10、50、100μg/ ml 三组）治疗组，在显微镜下用角膜环钻刻痕，以虹膜铲刮除角膜上皮... 详细信息

目的：研究重组人白细胞介素-1受体拮抗剂（RhIL-1ra）对眼角膜上皮细胞损伤的修复作用。方法将40只大鼠随机分为五组：对照组、模型组和 RhIL-1ra（10、50、100μg/ ml 三组）治疗组，在显微镜下用角膜环钻刻痕，以虹膜铲刮除角膜上皮，术后 RhIL-1ra 治疗组给予 RhIL-1ra（10、50、100μg/ ml）治疗，对照组和模型组给予氯化钠注射液治疗；治疗结束后在裂隙灯显微镜下观察角膜的损伤情况、he 染色观察角膜损伤程度和炎性细胞浸润情况。结果在裂隙灯显微镜下 RhIL-1ra 50μg/ ml 及 RhIL-1ra 100μg/ ml 治疗组着色区域较小，结膜充血、角膜水肿、房水混浊及虹膜表面新生血管等情况逐渐改善，其中以100μg/ ml 治疗组效果最为明显，且缺损面积得到一定修复。he 染色结果也表明，RhIL-1ra 治疗组可见细胞的排列以及形态等逐渐恢复，角膜损伤减轻，炎性细胞浸润也减少等现象。结论 RhIL-1ra 对损伤的眼角膜上皮细胞有恢复作用，可望成为治疗眼部炎症的新方法。

关键词：重组人白细胞介素-1 受体拮抗剂眼角膜上皮损伤修复

在线全文

学校读者我要写书评

暂无评论

杆式破片对高空气球膜体损伤影响因素分析

空天技术 2024年第3期2024卷 113-118页

作者：高燕婷张国伟何瑞龙雷文博中北大学机电工程学院

随着现代科技的发展，高空气球在民、军用领域应用极其广泛，同时也造成了颇多隐患。该研究主要通过数值模拟的方式揭示了高空气球在杆式破片冲击下的损伤规律及破坏形式，从而进一步分析了杆式破片在不同长径比和速度下对高空气球膜体... 详细信息

随着现代科技的发展，高空气球在民、军用领域应用极其广泛，同时也造成了颇多隐患。该研究主要通过数值模拟的方式揭示了高空气球在杆式破片冲击下的损伤规律及破坏形式，从而进一步分析了杆式破片在不同长径比和速度下对高空气球膜体损伤性能。结果表明：（1）气球膜体可以抵挡50 m/s以下的破片冲击，速度不足50 m/s时，只能由端面对气球膜体造成小破口；速度达到50 m/s时，方可将气球膜体穿透，且速度越高所造成的破坏面积越大。（2）杆式破片长径比增大会造成气球膜体的整体损伤面积变大，但对气球膜体被冲击的速度方向的位移量的影响不大。该研究可为民用和军用领域打击违规施放的高空气球提供一定的理论依据和技术支撑。

关键词：杆式破片高空气球膜体损伤分析低速冲击仿真模拟

放疗对人肝癌hepG2细胞凋亡及细胞周期的影响

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

陕西医学杂志 2007年第11期36卷 1481-1484页

作者：张力龚明玉何瑞龙承德医学院附属医院放疗科承德067000 承德医学院生物化学教研室

目的：探讨放疗对人肝癌hepG2细胞凋亡及细胞周期的影响。方法：采用6MV-X射线照射细胞,提取DNA进行琼脂糖凝胶电泳,观察细胞发生凋亡的情况,并采用免疫组织化学染色观察人肝癌细胞株中Bax和Bcl-2蛋白的表达。同时观察细胞周期改变情况... 详细信息

目的：探讨放疗对人肝癌hepG2细胞凋亡及细胞周期的影响。方法：采用6MV-X射线照射细胞,提取DNA进行琼脂糖凝胶电泳,观察细胞发生凋亡的情况,并采用免疫组织化学染色观察人肝癌细胞株中Bax和Bcl-2蛋白的表达。同时观察细胞周期改变情况。结果：照射后48h,2Gy、4Gy和6Gy出现明显DNA降解（“梯形结构”）,但对照组及8Gy和10Gy无“梯形结构”出现。Bax和Bcl-2蛋白的表达在时间和剂量之间存在交互作用（F=1.733,P=0.042;F=1.700,P=0.048）。4Gy照射剂量作用细胞48h后,hepG2细胞中Bax表达显著上调,而Bcl-2表达明显降低。4GyX线照射后培养6h,即可观察到G2/M期、S期阻滞,G2/M期阻滞峰值为正常的56倍,在-48h阻滞“崩溃”,细胞被释放,再度进入细胞周期或死亡。结论：6MV-X射线照射hepG2细胞,可诱使其产生凋亡,其机制可能通过促进Bax蛋白表达和抑制Bcl-2蛋白的表达来诱导肝癌细胞凋亡。4Gy照射引起的G2期阻滞释放可能与凋亡水平上调有关。

关键词：肝肿瘤,实验性/免疫学细胞凋亡放射疗法细胞周期 @Bax蛋白 @Bcl-2蛋白

Positive Correlation between PMS2 Deficiency and PD-L1 Expression in Pancreatic Cancer

在线全文

学校读者我要写书评

暂无评论

Advances in Bioscience and Biotechnology 2023年第2期14卷 74-90页

作者： Cailing Jiang Chunyan Dang ruilong he Limin Cheng Yiying Bai Xinyu Bai Xin Wang Qianhui Chen Hongbin Yang Zhengxin Zhang Xiaotong Zhang Yan Chen Qian Xu Lei Liu Li Zhang Department of Oncology The Affiliated Hospital of Chengde Medical University Chengde China Department of hepatobiliary Surgery The Affiliated Hospital of Chengde Medical University Chengde China Department of Immunology Chengde Medical University Chengde China Department of Pathology Chengde Medical University Chengde China

Background: Pancreatic cancer is one of the most lethal types of cancer, and immunotherapy has become a promising remedy with advancements in tumor immunology. However, predicting the clinical response to immunotherapy in pancreatic cancer remains a dilemma for clinicians. Methods: GEPIA database was used to analyze the differential expression of MMR and PD-L1 genes in 33 common cancer types including pancreatic cancer. The expression levels of MMR and PD-L1 genes were downloaded from the GEPIA and GEO databases to analyze the correlation between MMR genes and PD-L1, and the clinicopathological and survival information were downloaded from the TCGA databases to analyze the relationship between the expression of MMR, PD-L1 and clinicopathological characteristics, prognosis. Meanwhile, the tumor tissue samples of 41 patients with pancreatic cancer were collected, and the protein expression levels of MMR and PD-L1 were detected by immunohistochemical assay. Furthermore, we analyzed the correlation between MMR and PD-L1, and the correlation between the expression of MMR, PD-L1 and clinicopathological characteristics, prognosis of pancreatic cancer patients. Results: Bioinformatics analysis showed that MLH1, MLH3, MSH2, MSH3, and PMS2 were highly expressed in most cancer types including pancreatic cancer (P P = 0.012), clinical stage (I vs II: P = 0.016), MSH2 expression was related to clinical stage (P he correlation analysis revealed that the expressions MSH2, MLH1, PMS2 had strong correlations with PD-L1 both in GEPIA and GEO databases. Real-world data indicated that of the 41 pancreatic cancer patients, 5 cases had MLH1 deletion, 5 cases had MSH2 deletion, 4 cases had PMS2 deletion, and 12 cases had PD-L

关键词： Pancreatic Cancer PD-L1 PMS2 Mismatch Repair Protein Correlation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

X波段接收机强电磁脉冲的毁伤效应

火力与指挥控制 2024年第4期49卷 164-169页

作者：雷文博张国伟何瑞龙高燕婷中北大学机电工程学院太原030051

为解决低轨道侦察卫星的侦察行动使战场丧失隐蔽性的问题,采用电磁脉冲攻击卫星接收机的毁伤方法,利用电磁仿真软件CST、电路仿真软件ADS对电子目标进行建模,进行电磁脉冲辐照模拟试验。结果表明,高功率微波发射源以30 GW的发射功率、70... 详细信息

为解决低轨道侦察卫星的侦察行动使战场丧失隐蔽性的问题,采用电磁脉冲攻击卫星接收机的毁伤方法,利用电磁仿真软件CST、电路仿真软件ADS对电子目标进行建模,进行电磁脉冲辐照模拟试验。结果表明,高功率微波发射源以30 GW的发射功率、70 dB的发射增益,在距离目标400 km处,可造成接收机设备部分器件损毁;若以30 GW的发射功率、90 dB的发射增益、在距离目标400 km处,可造成接收机设备完全损毁。结果验证了电磁脉冲武器攻击低轨道微小卫星接收机方案的可行性。

关键词：侦察卫星接收天线高功率微波前门耦合毁伤效应数值仿真

在线全文

学校读者我要写书评

暂无评论

浅埋煤层地表动态变形规律实测研究

黄河水利职业技术学院学报 2023年第3期35卷 29-35页

作者：齐建伟查剑锋贺芮龙黄河水利职业技术学院河南开封475004 中国矿业大学环境与测绘学院江苏徐州221116

浅埋煤层高强度开采易诱发严重的开采沉陷灾害。为了分析浅埋煤层开采后地表沉陷特点,防治开采沉陷灾害,在马营煤矿9102综放工作面布置地表移动观测站,采用测回法,按照闭合测量路线进行动态变形监测,探讨了浅埋煤层地表移动动态特征。

关键词：浅埋煤层开采沉陷综放工作面地表移动观测站连续变形监测动态变形规律测回法闭合测量

Emerging direct current triboelectric nanogenerator for high-entropy mechanical energy harvesting

在线全文

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2024年第5期67卷 1297-1316页

作者： CheN Jie GUO ruilong GUO hengYu College of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China Department of Applied Physics Chongqing UniversityChongqing 401331China

In the era of the Internet of Things(IoT),the provision of sustainable power to distributed,mobile,and low-power-consumption electronic devices is a critical *** overcome this challenge,the triboelectric nanogenerator(TENG),a highly efficient high-entropy mechanical energy harvesting device,was developed in *** device enables the direct conversion of irregular and low-frequency mechanical energy into pulsed alternating current(AC)***,the incompatibility of most electronic devices with AC signals necessitates rectifier circuits or generators that deliver direct current(DC)*** recent years,DC-TENGs have undergone extensive development,achieving significant milestones in various application fields while also facing crucial challenges that require *** this review,three categories of DC-TENG devices with distinct operating mechanisms are comprehensively explored:multiphase coupling,mechanical rectification,and air *** typical structures and working mechanisms are thoroughly discussed,and specific output performance limitations,along with corresponding optimization strategies,are ***,the applications of DC-TENGs in various scenarios are ***,the challenges faced by DC-TENGs and potential solutions are analyzed to guide further advancements in this technology.

关键词： TENG direct current working mechanisms output performance limitations optimization strategies

Impact Analysis of MTD on the Frequency Stability in Smart Grid

在线全文

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2023年第1期10卷 275-277页

作者： Zhenyong Zhang ruilong Deng the State Key Laboratory of Public Big Data and the College of Computer Science and Technology Guizhou UniversityGuiyang 550025China IEEE the State Key Laboratory of Industrial Control Technology Zhejiang UniversityHangzhou 310027China

Dear Editor, In order to accommodate the effects of false data injection attacks(FDIAs), the moving target defense(MTD) strategy is recently proposed to enhance the security of the smart grid by perturbing branch susceptances. However, most pioneer work only focus on the defending performance of MTD in terms of detecting FDIAs and the impact of MTD on the static factors such as the power and economic losses.

关键词： Smart MTD Impact

L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2018年第3期4卷 329-338页

作者： Tongxin Wang Weilei Yao Qiongyu he Yafei Shao ruilong Zheng Feiruo Huang Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University

The liver is the most essential organ for the metabolism of ammonia, in where most of ammonia is removed by urea and glutamine synthesis. Regulated by leucine, glutamate dehydrogenase(GDH) catalyzes the reversible inter-conversion of glutamate to ammonia. To determine the mechanism of leucine regulating GDH, pigs weighing 20 ± 1 kg were infused for 80 min with ammonium chloride or alanine in the presence or absence of leucine. Primary pig hepatocytes were incubated with or without leucine. In the in vivo experiments with either ammonium or alanine as the nitrogen source, addition of leucine significantly inhibited ureagenesis and promoted the production of glutamate and glutamine in the perfused pig liver(P he increased phosphorylation levels of ribosomal protein S6 kinase 1(S6 K1) and ribosomal protein S6(S6). Interestingly, the leucine-induced m TORC1 pathway activation suitably correlated with increased GDH activity and decreased expression of *** results were observed in primary cultured hepatocytes. Notably, leucine exerted no significant change in GDH activity in SIRT4-deficient hepatocytes(P > 0.05), while m TORC1 signaling was *** exerted no significant changes in both GDH activity and SIRT4 gene expression in rapamycin treated hepatocytes(P > 0.05). In conclusion, L-leucine increases GDH activity and stimulates glutamate synthesis from different nitrogen sources by regulating m TORC1/SIRT4 pathway in the liver of pigs.

关键词： Glutamate dehydrogenase activity Glutamate synthesis L-leucine mTORC1/SIRT4 pathway Pig liver