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基于小RNA调控工具在蓝藻合成生物学中的应用

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中国生物工程杂志 2024年第2期44卷 176-189页

作者：王瑞彬董世瑞天津商业大学生物技术与食品科学学院、天津市食品生物技术重点实验室天津300134

基因调控工具在蓝藻合成生物学领域的应用尤为重要,可实现基因表达调控、工程株开发以及附加经济产物的生产。小RNA调控工具是基于小RNA的靶向特异性调控原理,并与合成生物学相结合,进行靶基因特异性调控的定量、全局调控工具。它将小RN... 详细信息

基因调控工具在蓝藻合成生物学领域的应用尤为重要,可实现基因表达调控、工程株开发以及附加经济产物的生产。小RNA调控工具是基于小RNA的靶向特异性调控原理,并与合成生物学相结合,进行靶基因特异性调控的定量、全局调控工具。它将小RNA与其靶标作为互作反应模块,并以诱导表达开关、支架序列与伴侣蛋白等作为辅助模块。这类工具已被应用于高附加经济产物合成与藻株对燃料与化学品的耐受性修饰。根据小RNA工具的不同调控特性,对近年来小RNA调控工具的种类、调控原理、辅助模块的选择与改造,以及在合成附加经济产物、提高蓝藻耐受性实验中获得的研究进展进行综述,探讨小RNA工具在应用中可能存在的问题及未来发展前景,为设计高效、精确的分子编辑工具提供参考。

关键词：合成生物学调控基因表达小RNA工具

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融合残差网络与注意力机制的草莓检测

中国农机化学报 2024年第1期45卷 266-273页

作者：王瑞彬杨世忠高升青岛理工大学信息与控制工程学院山东青岛266520

针对草莓果实因受到自然光光照、枝叶遮挡、果实间存在遮挡等因素,较难实现成熟草莓果实识别的现状,提出融合深度残差网络与注意力机制的成熟草莓目标检测算法。引用信息表达能力更强的深度残差网络Resnet50对SSD目标检测算法模型基础... 详细信息

针对草莓果实因受到自然光光照、枝叶遮挡、果实间存在遮挡等因素,较难实现成熟草莓果实识别的现状,提出融合深度残差网络与注意力机制的成熟草莓目标检测算法。引用信息表达能力更强的深度残差网络Resnet50对SSD目标检测算法模型基础骨干网络进行替换,对经过残差网络结构和新增卷积特征提取层得到信息特征提取图进行通道和空间方向的注意力机制方法处理,建立能准确实现成熟草莓目标检测的RC-SSD目标检测模型。试验结果表明,本文的RC-SSD算法模型对比Faster R-CNN、YOLOv3、SSD-VGG模型拥有较少的参数量,平均精度均值mAP分别提升46.05%、10.16%、5.77%,其中成熟草莓的识别精度达到99.04%。对比轻量化网络结构模型SSD-Mobilenetv2,RC-SSD算法模型在FPS相对于轻量化网络模型降低25帧的情况下,精度提升20.20%,FPS在GPU运行设备上达到86帧。

关键词：残差网络注意力机制损失函数目标检测草莓图像识别

全球气候治理达成“共识” 中国能源转型蹄疾步稳

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国际石油经济 2024年第1期32卷 46-47页

作者：王瑞彬中国国际问题研究院世界经济与发展研究所

2023年11月30日至12月12日,联合国第28次气候变化大会在阿拉伯联合酋长国迪拜举行,154个国家和政府的首脑参加了会议,198个缔约国最终达成具有里程碑意义的“阿联酋共识”。与会各国一致表示,将坚定发展可再生能源、降低化石能源消费、... 详细信息

2023年11月30日至12月12日,联合国第28次气候变化大会在阿拉伯联合酋长国迪拜举行,154个国家和政府的首脑参加了会议,198个缔约国最终达成具有里程碑意义的“阿联酋共识”。与会各国一致表示,将坚定发展可再生能源、降低化石能源消费、运用技术创新实现清洁转型、全面管控各类温室气体排放等,以“大幅、快速和持续地”减少温室气体排放,实现《巴黎协定》1.5℃温控目标要求。

关键词：全球气候治理可再生能源温室气体排放能源转型技术创新气候变化大会阿联酋《巴黎协定》

一种通用型牙种植导板在牙模上模拟后牙区种植的精度评价

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华西口腔医学杂志 2024年第3期42卷 365-371页

作者：王瑞斌胥铭章王兰郑紫阳邓云艺曾茂云袁玲玲彭培钊刘奇奇余科西南医科大学附属口腔医院种植科泸州646000

目的比较自主研制的通用型牙种植导板、3D打印数字化导板和自由手在牙模后牙区模拟种植的精度。方法选择10位初级种植医生分别以通用型自研导板、数字化导板和自由手3种方式在下颌模型(35、36、37及46牙缺失)的35、37和46牙位植入3枚种... 详细信息

目的比较自主研制的通用型牙种植导板、3D打印数字化导板和自由手在牙模后牙区模拟种植的精度。方法选择10位初级种植医生分别以通用型自研导板、数字化导板和自由手3种方式在下颌模型(35、36、37及46牙缺失)的35、37和46牙位植入3枚种植体,操作2次取平均值。利用锥形束CT评价种植体的实际位置与术前规划位置之间的顶端距离差、根尖距离差、深度差及角度差。结果自研导板组3个种植位点的顶端距离差、根尖距离差和其余两组无差异(P>0.05),深度差小于数字化导板组(P0.05),而数字化导板组37位点的深度差和角度差大于35位点(P<0.05)。结论自研的通用型牙种植导板的精度能满足临床后牙区种植的要求。

关键词：种植导板 3D打印偏差通用型导板数字化导板

A Gel-Based Solidification Technology for Large Fracture Plugging

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Fluid Dynamics & Materials Processing 2024年第3期20卷 563-578页

作者： Kunjian wang ruibin He Qianhua Liao Kun Xu Wen wang Kan Chen Tianjin Branch of CNOOC(China)Co. Ltd.Tianjin300450China Jingzhou Jiahua Technology Co. Ltd.Jingzhou434001China

Fault fractures usually have large openings and considerable extension. Accordingly, cross-linked gel materials aregenerally considered more suitable plugging agents than water-based gels because the latter often undergo contaminationvia formation water, which prevents them from being effective over long times. Hence, in this study, aset of oil-based composite gels based on waste grease and epoxy resin has been developed. These materials havebeen observed to possess high compressive strength and resistance to the aforementioned contamination, therebyleading to notable increase in plugging success rate. The compressive strength, thickening time, and resistance toformation water pollution of these gels have been evaluated indoors. The results show that the compressivestrength of the gel can reach 11 MPa;additionally, the related gelation time can be controlled to be more than3 h, thereby providing a safe construction time;Invasion of formation water has a small effect on the gel strengthand does not shorten the thickening time. All considered performance indicators of the oil-based gel confirm itssuitability as a plugging agent for fault fractures.

关键词： Drilling loss fault fracture oil-based gel compressive strength gel plugging

A new portable open-path instrument for ambient NH_(3) and on-road emission measurements

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Journal of Environmental Sciences 2024年第2期136卷 606-614页

作者： Wenhao Dai Shanshan wang Sanbao Zhang Jian Zhu Chuanqi Gu Zhibin Sun ruibin Xue Bin Zhou Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Department of Environmental Science and EngineeringFudan UniversityShanghai 200438China Institute of Eco-Chongming(IEC) Shanghai 202162China Institute of Atmospheric Sciences Fudan UniversityShanghai 200433China

Increased attentions to vehicle emission of NH_(3)have been paid since it is generally regarded as an important source in urban areas.Here,we developed a movable instrument based on Differential Optical Absorption Spectroscopy(DOAS)principle for detecting on-road NH_(3),which can avoid the losses in the sampling process attributed to the non-sampling methods.For this mobile DOAS,the temporal resolution,detection limit and relative error for NH_(3)were 1 min,2.29 ppbv and 4.57%±2.44%,respectively.By employed to the on-road measurements along the arterial highway in Shanghai,the spatial distributions of NH_(3)and NO were obtained,and their dependence of traffic and road conditions were studied.The slopes of linear regression between NH_(3)and NO were 0.40,0.02 and 0.07 on the Middle Ring Road,Outer Ring Road and Chongming Island Ring Road.It indicates that light gasoline vehicles(LGVs)were found to be the main contributor to NH_(3)emissions,while heavy-duty diesel vehicles(HDVs)mainly emitted NO.Based on the measured NH_(3)in the tunnel,the mileagebased NH_(3)emission factor per vehicle was estimated to be 17.9±6.3 mg/km.The reported open-path instrument can be broadly used in on-road pollutant monitoring or vehicle emissions,and the measurements can reveal the real situation of emission characteristics,even find the abnormal operations of vehicle catalyst system.

关键词： Ammonia Traffic emissions DOAS Emission factor Mobile measurements

A unified pre-training and adaptation framework for combinatorial optimization on graphs

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Science China Mathematics 2024年第6期67卷 1439-1456页

作者： ruibin Zeng Minglong Lei Lingfeng Niu Lan Cheng School of Computer Science and Technology University of Chinese Academy of SciencesBeijing100049China Faculty of Information Technology Beijing University of TechnologyBeijing100124China School of Economics and Management University of Chinese Academy of SciencesBeijing100190China School of Mathematics and Statistics Central South UniversityChangsha410075China

Combinatorial optimization(CO)on graphs is a classic topic that has been extensively studied across many scientific and industrial fields.Recently,solving CO problems on graphs through learning methods has attracted great attention.Advanced deep learning methods,e.g.,graph neural networks(GNNs),have been used to effectively assist the process of solving COs.However,current frameworks based on GNNs are mainly designed for certain CO problems,thereby failing to consider their transferable and generalizable abilities among different COs on graphs.Moreover,simply using original graphs to model COs only captures the direct correlations among objects,which does not consider the mathematical logicality and properties of COs.In this paper,we propose a unified pre-training and adaptation framework for COs on graphs with the help of the maximum satisfiability(Max-SAT)problem.We first use Max-SAT to bridge different COs on graphs since they can be converted to Max-SAT problems represented by standard formulas and clauses with logical information.Then we further design a pre-training and domain adaptation framework to extract the transferable and generalizable features so that different COs can benefit from them.In the pre-training stage,Max-SAT instances are generated to initialize the parameters of the model.In the fine-tuning stage,instances from CO and Max-SAT problems are used for adaptation so that the transferable ability can be further improved.Numerical experiments on several datasets show that features extracted by our framework exhibit superior transferability and Max-SAT can boost the ability to solve COs on graphs.

关键词： combinatorial optimization graph neural networks domain adaptation maximum satisfiability problem

Therapeutic effect of naloxone on radiation optic neurapathy Based on rabbit models

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Neural Regeneration Research 2007年第8期2卷 475-478页

作者： ruibin Liu Chuanfu wang Ophthalmology Center Union Hospital Changzhi Medical College Changzhi 046000 Shanxi Province China

BACKGROUND： Radiation therapy is widely used to treat tumor of brain; however, irradiation of radiation into eye tissues may easily cause ischemia and hypoxia in retina and optic nerve tissue so as to induce radiation optic neurapathy. Noloxone is a specific antagonist of opiate receptor, and it can change injured effect of f3 -endorphin. OBJECTIVE： To observe the axoplasmic transport of optic nerve at various phases after radiation injury so as to investigate the mechanism and regularity of optic nerve injury; meanwhile, to verify the therapeutic effects of naloxone on radiation optic neurapathy. DESIGN： Randomized controlled animal study. SETTINGS： Medical College of Qingdao University, Changzhi Medical College. MATERIALS： A total of 40 healthy adult New Zealand rabbits, weighing 2 - 2.5 kg, of either gender, were checked by using slit lamp and ophthalmoscop before radiation in order to exclude eye diseases. FCC-7000 vertical kilocurie ^60Co therapeutic machine was made in Yantai, China; in addition, naloxone was provided by Beijing Sihuan Pharmaceutical Factory. METHODS： The experiment was carried out in the Animal Experimental Laboratory, Medical College of Qingdao University from January 2005 to December 2006. The experimental rabbits were randomly divided into radiation group （n =16）, treatment group （n =16）, blank control group （n=4） and injured control group （n=4）. Except blank control group, rabbits in other three groups were irradiated in as the center of optic chiasma including the area of optic nerve by using ^60Co therapeutic machine. Radioactive source was 85 cm away from head, and the size of operative field was 5 cm × 5 cm. The radiation was performed once a day with the dosage of 3 Gy for 8 days in total. The total dosage was 24 Gy. When radioactive dosage reached 24 Gy, 2 mg/kg naloxone was dropped into ear vein of rabbits in the treatment group once a day for 10 days in total. Rabbits in the injured control group were only irradiated but not given any drugs. MAIN OUTCOME MEASURES： At 1, 10, 30

关键词： naloxone optic nerve injuries radioactivity

Typhoon-and pollution-driven enhancement of reactive bromine in the mid-latitude marine boundary layer

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National Science Review 2024年第4期11卷 93-101页

作者： Shanshan wang Qinyi Li Ruifeng Zhang Anoop Sharad Mahajan Swaleha Inamdar Nuria Benavent Sanbao Zhang ruibin Xue Jian Zhu Chenji Jin Yan Zhang Xiao Fu Alba Badia Rafael P.Fernandez Carlos A.Cuevas Tao wang Bin Zhou Alfonso Saiz-Lopez Institute for Interdisciplinary Science (ICB) National Research Council (CONICET) Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Institute of Eco-Chongming(IEC) Department of Atmospheric Chemistry and Climate Institute of Physical Chemistry Blas CabreraCSIC Department of Civil and Environmental Engineering The Hong Kong Polytechnic University Environment Research Institute Shandong University Centre for Climate Change Research Indian Institute of Tropical MeteorologyMinistry of Earth Sciences Department of Chemistry University of Colorado Boulder Institute of Environment and Ecology Tsinghua Shenzhen International Graduate SchoolTsinghua University Sostenipra Research Group Institute of Environmental Science and Technology(ICTA)Universitat Autonoma de Barcelona(UAB)

Tropospheric reactive bromine is important for atmospheric chemistry,regional air pollution,and global climate.Previous studies have reported measurements of atmospheric reactive bromine species in different environments,and proposed their main sources,e.***.sea-salt aerosol（SSA）,oceanic biogenic activity,polar snow/ice,and volcanoes.Typhoons and other strong cyclonic activities（e.***.hurricanes） induce abrupt changes in different earth system processes,causing widespread destructive effects.However,the role of typhoons in regulating reactive bromine abundance and sources remains unexplored.Here,we report field observations of bromine oxide（BrO）,a critical indicator of reactive bromine,on the Huaniao Island（HNI）in the East China Sea in July 2018.We observed high levels of BrO below 500 m with a daytime average of9.7±4.2 pptv and a peak value of ～26 pptv under the influence of a typhoon.Our field measurements,supported by model simulations,suggest that the typhoon-induced drastic increase in wind speed amplifies the emission of SSA,significantly enhancing the activation of reactive bromine from SSA debromination.We also detected enhanced BrO mixing ratios under high NOxconditions（ppbv level） suggesting a potential pollution-induced mechanism of bromine release from SSA.Such elevated levels of atmospheric bromine noticeably increase ozone destruction by as much as ～40% across the East China Sea.Considering the high frequency of cyclonic activity in the northern hemisphere,reactive bromine chemistry is expected to play a more important role than previously thought in affecting coastal air quality and atmospheric oxidation capacity.We suggest that models need to consider the hitherto overlooked typhoon-and pollution-mediated increase in reactive bromine levels when assessing the synergic effects of cyclonic activities on the earth system.

关键词： reactive bromine atmospheric chemistry marine boundary layer atmospheric oxidation capacity marine emission