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Overexpressed miRNA-155 dysregulates intestinal epithelial apical junctional complex in severe acute pancreatitis

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World Journal of Gastroenterology 2013年第45期19卷 8282-8291页

作者： rui Tian rui-lan wang Hui Xie Wei Jin Kang-Long Yu Intensive Care Unit First People's Hospital Shanghai Jiaotong University

AIM:To investigate whether miRNA-155(miR-155)dysregulates apical junctional complex(AJC)protein expression in experimental severe acute pancreatitis(SAP).METHODS:Twenty-four male BALB/c mice were randomly assigned to two groups:the SAP group(n=12)receiving sequential intraperitoneal injection of 50μg/kg caerulein and 10 mg/kg lipopolysaccharide over 6h,and the control group(n=12)receiving intraperitoneal injection of normal *** were sacrificed3 h following the last injection for collection of blood samples and pancreas and distal ileal segment *** pancreas and intestine histology was used to assess SAP pathology and intestinal epithelial barrier *** of serum amylase,diamine oxidase(DAO),and tumor necrosis factor(TNF)-αwere determined using commercial *** RNA samples were isolated from intestinal epithelial specimens and reversely transcribed into ***-155 and RhoA mRNA expression profiles were determined using quantitative real-time polymerase chain *** genes for miR-155 were predicted using the miRTarBase database,RNA22 and PicTar computational *** blotting was performed to quantitate the protein expression levels of the target gene RhoA,as well as zonula occludens(ZO)-1 and E-cadherin,two AJC component ***:Intraperitoneal injection of caerulein and lipopolysaccharide successfully induced experimental acute pancreatic damage(SAP vs control,10.0±2.0vs 3.2±1.2,P<0.01)and intestinal epithelial barrier damage(3.2±0.7 vs 1.4±0.7,P<0.01).Levels of serum amylase(21.6±5.1 U/mL vs 14.3±4.2 U/mL,P<0.01),DAO(21.4±4.1 mg/mL vs 2.6±0.8 mg/mL,P<0.01),and TNF-α(61.0±15.1 ng/mL vs 42.9±13.9 ng/mL,P<0.01)increased significantly in SAP mice compared to those in control ***-155 was significantly overexpressed in SAP intestinal epithelia(1.94±0.50 fold vs 1.03±0.23 fold,P<0.01),and RhoA gene containing three miR-155-specific binding sites in the three prime untranslated regions was one of the targe

关键词： miRNA-155 Severe acute pancreatitis Intestinal barrier dysfunction Apical junctional complex

Association between autoimmune gastritis and gastric polyps:Clinical characteristics and risk factors

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 73-87页

作者： Jing-Zheng Jin Xiao Liang Shu-Peng Liu rui-lan wang Qing-Wei Zhang Yu-Feng Shen Xiao-Bo Li Division of Gastroenterology and Hepatology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Shanghai Institute of Digestive DiseasesShanghai 200001China Division of Gastroenterology and Hepatology Renji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Sichuan Armed Police Corps HospitalLeshan 610041Sichuan ProvinceChina Division of Gastroenterology and Hepatology NHC Key Laboratory of Digestive DiseasesRenji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Gastroenterology Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai Institute of Digestive DiseasesShanghai 200127China

BACKGROUND The relationship between autoimmune gastritis(AIG)and gastric polyps(GPs)is not well *** To explore the clinical characteristics and risk factors of AIG with GPs in *** This double center retrospective study included 530 patients diagnosed with AIG from July 2019 to July *** collected clinical,biochemical,serological,and demographic data were of each *** regression analyses,both multivariate and univariate,were conducted to pinpoint independent risk factors for GPs in patients with AIG *** operating characteristic curves were utilized to establish the optimal cutoff values,sensitivity,and specificity of these risk factors for predicting GPs in patients with *** Patients with GPs had a higher median age than those without GPs[61(52.25-69)years vs 58(47-66)years,P=0.006].The gastrin-17 levels were significantly elevated in patients with GPs compared with those without GPs[91.9(34.2-138.9)pmol/mL vs 60.9(12.6-98.4)pmol/mL,P<0.001].Additionally,the positive rate of parietal cell antibody(PCA)antibody was higher in these patients than in those without GPs(88.6%vs 73.6%,P<0.001).Multivariate and univariate analyses revealed that PCA positivity[odds ratio(OR)=2.003,P=0.017],pepsinogen II(OR=1.053,P=0.015),and enterochromaffin like cells hyperplasia(OR=3.116,P<0.001)were significant risk factors for GPs,while pepsinogen I was identified as a protective *** PCA positivity and enterochromaffin like cells hyperplasia are significant risk factor for the development of GPs in patients with *** gastrin-17 levels may also play a role in this *** findings suggest potential targets for further research and therapeutic intervention in managing GPs in patients with AIG.

关键词： Autoimmune gastritis Gastric polyps Neuroendocrine tumor Risk factors Nomogram

Ventilator management for acute respiratory distress syndrome associated with avian infl uenza A(H7N9) virus infection: A case series

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World Journal of Emergency Medicine 2018年第2期9卷 118-124页

作者： Hui Xie Zhi-gang Zhou Wei Jin Cheng-bin Yuan Jiang Du Jian Lu rui-lan wang Department of Critical Care Medicine Shanghai General Hospital Shanghai Jiaotong University School of Medicine

BACKGROUND: Data on the mechanical ventilation(MV) characteristics and radiologic features for the cases with H7 N9-induced ARDS were still ***: We describe the MV characteristics and radiologic features of adult patients with ARDS due to microbiologically confirmed H7 N9 admitted to our ICU over a 3-month ***: Eight patients(mean age 57.38±16.75; 5 male) were diagnosed with H7 N9 in the first quarter of 2014. All developed respiratory failure complicated by acute respiratory distress syndrome(ARDS), which required MV in ICU. The baseline APACHE II and SOFA score was 11.77±6.32 and 7.71±3.12. The overall CT scores of the patients was 247.68±34.28 and the range of CT scores was 196.3–294.7. The average MV days was 14.63±6.14, and 4 patients required additional rescue therapies for refractory hypoxemia. Despite these measures, 3 patients ***: In H7 N9-infected patients with ARDS, low tidal volume strategy was the conventional mode. RM as one of rescue therapies to refractory hypoxemia in these patients with serious architectural distortion and high CT scores, which could cause further lung damage, may induce bad outcomes and requires serious consideration. Prone ventilation may improve mortality, and should be performed at the early stage of the disease, not as a rescue therapy.

关键词： Acute respiratory distress syndrome Influenza A virus H7N9 Viral pneumonia Mechanical ventilation Recruitment maneuvers Prone positioning

Salvage of venoarterial extracorporeal membrane oxygenation following prolonged cardiopulmonary resuscitation and failure of catheter-directed thrombectomy for massive pulmonary embolism in a young patient

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World Journal of Emergency Medicine 2023年第2期14卷 161-164页

作者： Ge Yu Dong-po Wei Hui Xie Jian Lu Jun-li Zhao Shuang Li Wei Chen rui-lan wang Department of Emergency and Critical Care Medicine Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghai 200080China Department of Cardiology Shanghai Municipal Hospital of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghai 200071China Department of Cardiology Shanghai Fourth People’s Hospital Affiliated to Tongji UniversityShanghai 200434China

Dear editor,Acute massive pulmonary embolism(PE) is a common life-threatening disease with high mortality of up to 30%–50%.[1,2]Potential heterogeneous reasons for PE remain controversial, and its treatment strategie... 详细信息

关键词： Acute mortality massive

Effect of Platelet-derived P-selectin on Neutrophil Recruitment in a Mouse Model of Sepsis-induced Acute Kidney Injury

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Chinese Medical Journal 2017年第14期130卷 1694-1699页

作者： Xiu-Hua Li Yong-Bing Qian Xiao-Xiao Meng rui-lan wang Department of Critical Care Medicine Shanghai General Hospital of Nanjing Medical University Shanghai 201620 China Emergency Center Shanghai East Hospital Tongji University School of Medicine Shanghai 200120 China

Background：Acute kidney injury （AKI） is a severe disease in critically ill *** infiltration into kidney was associated with the development of AKI,and P-selectin may be involved in the process of neutrophil recruitment in *** study aimed to explore the potential effect of platelet-derived P-selectin on neutrophil recruitment in a mouse model of sepsis-induced ***：A total of 30 C57BL/6 male mice were divided into five groups （n =6 in each）：sham group,sepsis group,anti-Ly6G group,anti-P-selectin group,and platelet depletion *** was induced by cecal ligation and *** creatinine concentration and platelet activity were measured by biochemical detector and flow cytometry,*** and pathological features were analyzed using hematoxylin-eosin （HE） and immunohistochemi stry （IHC） staining,*** （M PO） activity was detected with MPO *** t-test was used for data ***：Serum creatinine increased significantly in septic group compared to sham group （2.68 ± 0.27 mg/dl vs.0.82 ± 0.19 mg/dl,t =12.06,P =0.0000） but attenuated in antibodies-treated animals compared to septic group （anti-Ly6G：1.62 ± 0.30 mg/dl vs.2.68 ± 0.27 mg/dl,t =5.76,P=0.0004;anti-P-selectin：1.76 ± 0.31 mg/dl vs.2.68 ± 0.27 mg/dl,t =4.92,P =0.0012;and platelet depletion：1.93 ± 0.29 mg/dl vs.2.68 ± 0.27 mg/dl,t =4.14,P =0.0032）.Platelet amount significantly decreased compared to sham group （658.20 ± 60.64 × 109/L vs.822.00 ± 48.60 × 109/L,t =4.71,P =0.0015） in septic mice,especially in platelet depletion group （240.80 ± 44.98 × 109/L vs.822.00 ± 48.60 × 109/L,t =19.63,P =0.0000）.P-selectin activity was significantly increased in septic group compared to sham group （16.54 ± 1.60% vs.1.90 ± 0.29%,t =15.64,P =0.0000） but decreased significantly in platelet depletion group compared to septic group （3.62 ± 0.68% vs.16.54 ± 1.60%,t =12.89,P =0.0002）.IHC analysis shown that neutrophil infiltration increased

关键词： Acute Kidney Injury Neutrophils P-Selectin Sepsis

Effects of dynamic ventilatory factors on ventilatorinduced lung injury in acute respiratory distress syndrome dogs

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World Journal of Emergency Medicine 2012年第4期3卷 287-293页

作者： rui-lan wang Kan Xu Kang-long Yu Xue Tang Hui Xie Intensive Care Unit First People＇s Hospital Affiliated to Shanghai Jiao Tong University Shanghai 201620 China

BACKGROUND： Mechanical ventilation is a double-edged sword to acute respiratory distress syndrome （ARDS） including lung injury, and systemic inflammatory response high tidal volumes are thought to increase mortality. The objective of this study is to evaluate the effects of dynamic ventilatory factors on ventilator induced lung injury in a dog model of ARDS induced by hydrochloric acid instillation under volume controlled ventilation and to investigate the relationship between the dynamic factors and ventilator-induced lung injuries （VILI） and to explore its potential ***： Thirty-six healthy dogs were randomly divided into a control group and an experimental group. Subjects in the experimental group were then further divided into four groups by different inspiratory stages of flow. Two mL of alveolar fluid was aspirated for detection of IL-8 and TNF-α. Lung tissue specimens were also extracted for total RNA, IL-8 by western blot and observed under an electronic ***： IL-8 protein expression was significantly higher in group B than in groups A and D. Although the IL-8 protein expression was decreased in group C compared with group B, the difference was not statistically significant. The TNF-a ray degree of group B was significantly higher than that in the other groups （P〈0.01）, especially in group C （P〉0.05）. The alveolar volume of subjects in group B was significantly smaller, and cavity infiltration and cell autolysis were marked with a significant thicker alveolar septa, disorder of interval structures, and blurring of collagenous and elastic fiber structures. A large number of necrotic debris tissue was observed in group ***： Mechanical ventilation with a large tidal volume, a high inspiratory flow and a high ventilation frequency can cause significant damage to lung tissue structure. It can significantly increase the expression of TNF-α and IL-8 as well as their mRNA expression. Furthermore, the results of our study showed t

关键词： Acute respiratory distress syndrome Dynamic factors Inspiratory flow Ventilator-induced lung injury

Effect of ulinastatin on paraquat-induced-oxidative stress in human type Ⅱ alveolar epithelial cells

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World Journal of Emergency Medicine 2013年第2期4卷 133-137页

作者： Xiao-xiao Meng rui-lan wang Shan Gao Hui Xie Jiu-ting Tan Yong-bin Qian Department of Critical Care Medicine Shanghai First People's HospitalSchool of MedicineShanghai Jiao Tong University

BACKGROUND： Ulinastatin （UTI） is a urinary trypsin inhibitor extracted and purified from urine of males. This study aimed to explore the effects of UTI on paraquat-induced-oxidative stress in human type II alveolar epithelial cells. METHODS： The human type II alveolar epithelial cells, A549 cells, were cultured in vitro. The A549 cells were treated with different concentrations of paraquat （200, 400, 600, 800, 1 000, 1 200 pmol/L） and ulinastatin（0, 2 000, 4 000, 6 000, 8 000 U/mL） for 24 hours, the cell viability was measured by cell counting kit-8 and the median lethal concentration was selected. In order to establish an in vitro model of paraquat intoxication and to determine the safe dose of ulinastatin, we calculated LD50 using cell counting kit-8 to determine the survival rate of the cells. A549 cells were divided into normal control group, paraquat group and paraquat＋ulinastatin group. The levels of malondialdehyde （MDA） and myeloperoxidase （MPO） were detected by biochemistry colorimetry, while the level of reactive oxygen spies （ROS） was detected by DCFH-DA assay. RESULTS： The survival rate of A549 cells treated with different concentrations of paraquat decreased in a concentration-dependent manner. Whereas there was no decrease in the survival rate of cells treated with 0-4 000 U/mL ulinastatin. The levels of MDA, MPO, and ROS were significantly higher in the paraquat group than in the normal control group after 24-hour-exposure. And the survival rate of the paraquat＋ulinastatin group was higher than that of the paraquat group, but lower than that of the normal control group. The levels of MDA, MPO, and ROS were lower than those of the paraquat group. CONCLUSION： Ulinastatin can alleviate the paraquat-induced A549 cell damage by reducing oxidative stress.

关键词： Ulinastatin Paraquat Oxidative stress A549 ce

Expert consensus on the use of human serum albumin in critically ill patients

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Chinese Medical Journal 2021年第14期134卷 1639-1654页

作者： Yue-Tian Yu Jiao Liu Bo Hu rui-lan wang Xiang-Hong Yang Xiu-Ling Shang Gang wang Chang-Song wang Bai-Ling Li Ye Gong Sheng Zhang Xin Li Lu wang Min Shao Mei Meng Feng Zhu You Shang Qiang-Hong Xu Zhi-Xiong Wu De-Chang Chen Department of Critical Care Medicine Ren Ji HospitalShanghai Jiao Tong University School of MedicineShanghai 200001China Department of Critical Care Medicine Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Critical Care Medicine Zhongnan Hospital of Wuhan UniversityWuhanHubei 430071China Department of Critical Care Medicine Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 201620China Department of Critical Care Medicine Zhejiang Provincial People’s Hospital(People’s Hospital of Hangzhou Medical College)HangzhouZhejiang 310014China Department of Critical Care Medicine Fujian Provincial HospitalShengli Clinical Medical College of Fujian Medical UniversityFuzhouFujian 350001China Department of Critical Care Medicine The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi 710004China Department of Critical Care Medicine Harbin Medical University Cancer HospitalHarbinHeilongjiang 150081China Department of Cardiovascular Surgery Changhai HospitalNaval Military Medical University(The Second Military Medical University)Shanghai 200433China Department of Critical Care Medicine Department of NeurosurgeryHuaShan HospitalFudan UniversityShanghai 200040China Department of Cardiovascular Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Critical Care Medicine The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China Department of Burn and Trauma Intensive Care Unit The First Affiliated HospitalNaval Medical UniversityShanghai 200433China Department of Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Critical Care Medicine Zhejiang HospitalHangzhouZhejiang 310013China Department of Critical Care Medicine Huadong HospitalFudan UniversityShanghai 20

Introduction Human serum albumin(HSA)is a non-glycosylated,negatively charged,single-chain polypeptide composed of 585 amino acid residues with a relative molecular mass of 66.438 *** is synthesized by the liver at a rate of approximately 200 mg·kg^(-1)·day^(-1),with a half-life of 21 days,and subjected to catabolism in the muscles,liver,and kidneys at a rate of 4%per day.[1]Albumin,accounting for 60%of the total plasma protein,has various physiological functions,[2]such as maintaining 70%to 80%of effective plasma colloid osmotic pressure,coordinating vascular endothelial integrity,anti-oxidant and anti-inflammatory activities,maintaining the acidbase balance,and participating in the transport,distribution,and metabolism of a variety of endogenous and exogenous substances.

关键词： metabolism endogenous critically

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu rui wang Hanyue Zhang Xiou wang Sijia Zhou Bo Ma Yan Luan Xin wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells