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Single-cell transcriptomic landscape of peripheral blood cells provides insights into adaptation of red-eared sliders(Trachemys scripta elegans)

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Integrative Zoology 2024年第3期19卷 468-479页

作者： rui GUO Guangwei MA Xiaofei ZHAI Haitao shi Jichao WANG Ministry of Education Key Laboratory for Ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikouChina

Red-eared sliders(Trachemys scripta elegans),as one of the 100 most threatening aliens,have stronger immunity than the native species in response to environmental *** cells are an important component of immunity in the ***,the blood cell researches of turtle are still in the traditional blood cell classification and morphological structure ***,turtle granulocytes cannot be accurately identified using traditional ***-cell RNA sequencing techniques have been successfully implemented to study cells based on the mRNA expression patterns of each *** present study profiled the transcriptomes of peripheral blood cells in red-eared sliders to construct a single-cell transcriptional landscape of the different cell types and explored environmental adaptation mechanism from the perspective of *** 14 transcriptionally distinct clusters(platelets,erythrocytes1,erythrocytes2,CSF1R monocytes,POF1B monocytes,neutrophils,GATA2high basophils,GATA2low basophils,CD4 T cells,CD7 T cells,B cells,ACKR4 cells,serotriflin cells,and ficolin cells)were identified in the peripheral blood cells of the red-eared *** particular,a subtype of erythrocytes(erythrocytes1)that expressed immune signals was *** blood cells were grouped into three lineages:platelet,erythroid/lymphoid,and myeloid cell ***,based on differentiation trajectory and up-regulated gene expression,ACKR4 cells were newly identified as lymphocytes,and serotriflin and ficolin cells as *** single-cell transcriptional atlas of the peripheral blood cells in red-eared sliders provided in the present study will offer a comprehensive transcriptome reference for the exploration of physiological and pathological hematology in this species.

关键词： blood cells single-cell RNA-seq Trachemys scripta elegans

On the Performances of Estimating Stellar Atmospheric Parameters from CSST Broad-band Photometry

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Research in Astronomy and Astrophysics 2024年第4期24卷 161-173页

作者： rui-Feng shi Yang Huang Xin-Yi Li Hua-Wei Zhang School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China South-Western Institute for Astronomy Research Yunnan UniversityKunming 650500China CAS Key Lab of Optical Astronomy Key Laboratory of Space Astronomy and TechnologyNational Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China College of Physics and Electronic Engineering Qilu Normal UniversityJinan 250200China Department of Astronomy School of PhysicsPeking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China

Deriving atmospheric parameters of a large sample of stars is of vital importance to understand the formation and evolution of the Milky *** surveys,especially those with near-ultraviolet filters,can offer accurate measurements of stellar parameters,with the precision comparable to that from low/medium resolution *** this study,we explore the capability of measuring stellar atmospheric parameters from Chinese Space Station Telescope(CSST)broad-band photometry(particularly in the near-ultraviolet bands),based on synthetic colors derived from model *** find that colors from the optical and near-ultraviolet filter systems adopted by CSST show significant sensitivities to the stellar atmospheric parameters,especially the *** to our mock data tests,the precision of the photometric metallicity is quite high,with typical values of0.17 and 0.20 dex for dwarf and giant stars,*** precision of the effective temperature estimated from broad-band colors are within 50 K.

关键词： methods:data analysis stars:abundances surveys

Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform

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Chinese Chemical Letters 2024年第2期35卷 441-445页

作者： shi Hu rui Hao Zitong Yu Huitao Zhang Hui Yang Bionic Sensing and Intelligence Center Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and ***,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,***,we present a microfluidic platform to enhance EVs’labelling efficiency and improve their *** platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing *** cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1%relative improvement than conventional co-incubation method and a lower background ***,this technique maintains EVs’size,morphology and biological *** the recipient cells uptake the EVs treated by the microfluidic platform,the spatial and temporal distribution of EVs in the cells are clearly *** results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantification and analysis.

关键词： Microfluidic platform Extracellular vesicles Labelling PKH dye Flow cytometry

A real-time intelligent lithology identification method based on a dynamic felling strategy weighted random forest algorithm

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Petroleum Science 2024年第2期21卷 1135-1148页

作者： Tie Yan rui Xu shi-Hui Sun Zhao-Kai Hou Jin-Yu Feng School of Petroleum Engineering Northeast Petroleum UniversityDaqing163318HeilongjiangChina Sanya Offshore Oil&Gas Research Institute Northeast Petroleum UniversitySanya572025HainanChina

Real-time intelligent lithology identification while drilling is vital to realizing downhole closed-loop drilling. The complex and changeable geological environment in the drilling makes lithology identification face many challenges. This paper studies the problems of difficult feature information extraction,low precision of thin-layer identification and limited applicability of the model in intelligent lithologic identification. The author tries to improve the comprehensive performance of the lithology identification model from three aspects: data feature extraction, class balance, and model design. A new real-time intelligent lithology identification model of dynamic felling strategy weighted random forest algorithm(DFW-RF) is proposed. According to the feature selection results, gamma ray and 2 MHz phase resistivity are the logging while drilling(LWD) parameters that significantly influence lithology identification. The comprehensive performance of the DFW-RF lithology identification model has been verified in the application of 3 wells in different areas. By comparing the prediction results of five typical lithology identification algorithms, the DFW-RF model has a higher lithology identification accuracy rate and F1 score. This model improves the identification accuracy of thin-layer lithology and is effective and feasible in different geological environments. The DFW-RF model plays a truly efficient role in the realtime intelligent identification of lithologic information in closed-loop drilling and has greater applicability, which is worthy of being widely used in logging interpretation.

关键词： Intelligent drilling Closed-loop drilling Lithology identification Random forest algorithm Feature extraction

Suppression of low-frequency ultrasound broadband vibration using star-shaped single-phase metamaterials

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Defence Technology（防务技术） 2024年第4期34卷 217-224页

作者： rui Zhao Jian Zheng Jin Guo Yunbo shi Hengzhen Feng jun Tang jun Liu Science and Technology on Electronic Test&Measurement Laboratory North University of ChinaTaiyuan 030051ShanxiChina School of Mechatronical Engineering Beijing Institute of TechnologyBeijing 100081China

In order to suppress the low-frequency ultrasound vibration in the broadband range of 20 k Hz—100 k Hz,this paper proposes and discusses an acoustic metamaterial with low-frequency ultrasound vibration attenuation properties,which is configured by hybrid arc and sharp-angle convergent star-shaped *** effect of the dispersion relation and the bandgap characteristic for the scatterers in star-shaped are simulated and *** target bandgap width is extended by optimizing the geometry parameters of arc and sharp-angle convergent *** proposed metamaterial configured by optimized hybrid lattices exhibits remarkable broad bandgap characteristics by bandgap complementarity,and the simulation results verify a 99%vibration attenuation amplitude can be obtained in the frequency of20 k Hz—100 k *** the fabrication of the proposed hybrid configurational star-shaped metamaterial by 3D printing technique,the transmission loss experiments are performed,and the experimental results indicate that the fabricated metamaterial has the characteristics of broadband vibration attenuation and an amplitude greater than 85%attenuation for the target *** results demonstrate that the hybrid configurational star-shaped metamaterials can effectively widen the bandgap and realize high efficiency attenuation,which has capability for the vibration attenuation in the application of highprecise equipment.

关键词： Star-shaped metamaterials Broadband Vibration attenuation Low-frequency ultrasound vibration Transmission loss

Process and performance of DAAF microspheres prepared by continuous integration from synthesis to spherical coating based on microfluidic system

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Defence Technology（防务技术） 2024年第2期32卷 629-643页

作者： Bidong Wu Jiahui shi Mengsen Wei rui Zhu Yi Liu Jinqiang Zhou Chongwei An Jingyu Wang School of Environment and Safety Engineering North University of ChinaTaiyuan 030051China Shanxi Engineering Technology Research Centre for Ultrafine Powder North University of ChinaTaiyuan 030051China School of Mechatronical Engineering Beijing Institute of TechnologyBeijing 100081China

In order to improve the energy output consistency of 3, 3’-diamino-4, 4’-azoxyfurazan(DAAF) in the new insensitive booster and the safety and efficiency in the preparation process, a continuous preparation system of DAAF from synthesis to spherical coating was designed and established in this paper, which combined ultrasonic micromixing reaction with microdroplet globular template. In the rapid micromixing stage, the microfluidic mixing technology with ultrasonic was used to synergistically strengthen the uniform and rapid mass transfer mixing reaction between raw materials to ensure the uniformity of DAAF particle nucleation-growth, and to prepare high-quality DAAF crystals with uniform structure and morphology and concentrated particle size distribution. In the microdroplet globular template stage, the microfluidic droplet technology was used to form a droplet globular template with uniform size under the shear action of the continuous phase of the dispersed phase solution containing DAAF particles and binder. The size of the droplet template was controlled by adjusting the flow rate ratio between the continuous phase and the dispersed phase. In the droplet globular template, with the diffusion of the solvent in the dispersed phase droplets, the binder precipitates to coat the DAAF into a ball, forming a DAAF microsphere with high sphericity, narrow particle size distribution and good monodispersity. The problem of discontinuity and DAAF particle suspension in the process was solved, and the coating theory under this process was studied. DAAF was coated with different binder formulations of fluororubber(F2604), nitrocellulose(NC) and NC/glycidyl azide polymer(GAP), and the process verification and evaluation of the system were carried out. The balling effects of large, medium and small droplet templates under different binder formulations were studied. The scanning electron microscope(SEM) results show that the three droplet templates under the three binder formul

关键词： DAAF Micromixing technology Microdroplet technology Synthesis Spherical coating Continuousization

Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment

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Nano Research 2024年第8期17卷 7376-7393页

作者： Aoao Wang Xinbo Ma Yafeng Yang Guoliang shi Liwei Han Xiantong Hu rui shi jun Yan Quanyi Guo Yantao Zhao Senior Department of Orthopedics the Fourth Medical Center of PLA General HospitalBeijing 100048China Department of Chemistry Capital Normal UniversityBeijing 100048China National Center for Orthopaedics Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China Xijing 986 Hospital Department The Fourth Military Medical UniversityXi'an 710032China Institute of Orthopedics Chinese PLA General HospitalBeijing Key Laboratory of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma&War Injuries PLABeijing 100853China

The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and *** this study,we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial,immunomodulatory,and osteogenic effects using electrospinning *** scaffold’s piezoelectric signal output under ultrasound(US)control can be similar to the physiological electrical signals of healthy bone tissue,creating a truly biomimetic electrical microenvironment in the bone *** vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation,migration,and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ***,the scaffold exhibits inhibitory effects on the growth of *** and ***,as well as the ability to induce M2 macrophage polarization,indicating potent antibacterial and immunomodulatory *** vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats,effectively inhibit bacterial colonization,and reduce inflammatory ***,this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing,thus providing a simple and effective solution for bone defects.

关键词： piezoelectricity bone defect bacterial infection ZnO nanoparticles immunomodulation osteogenesis

ShCTR1 Interacts with ShRBOH1 to Positively Regulate Aerenchyma Formation in Saussurea inversa through ROS Mediation

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Phyton-International Journal of Experimental Botany 2024年第5期93卷 1023-1042页

作者： Wubin Dai Xiuting ju Guomin shi Jialei Guo Tao He School of Agriculture and Animal Husbandry Qinghai UniversityXining810016China Key Laboratory of Landscape Plants of Qinghai Province Qinghai UniversityXining810016China School of Ecol-Environmental Engineering Qinghai UniversityXining810016China State Key Laboratory of Plateau Ecology and Agriculture Qinghai UniversityXining810016China

Saussurea inversa is one of the Tibetan medicine with developed *** this study,we investigated the function of the ShCTR1 gene related to the formation of *** study of the occurrence and formation of aerenchyma in *** has certain theoretical significance in revealing the special mechanism of alpine plants adapting to the *** results of yeast two-hybrid experiments showed that *** ShCTR1 interacted with the ShRBOH1,which is a key producer of reactive oxygen species(ROS),and it was hypothesized that ShCTR1 regulation of aerenchyma formation in *** mainly mediated by *** induction and inhibition experiments were carried out on *** seedlings,it was shown that ROS inducer(AT)and Ethene(ETH)induced the formation of aerenchyma in *** well in roots,stems,and leaves,and Ethylene inhibitor(1-MCP)and ROS inhibitor DPI(diphenylene iodonium)inhibited the formation of aerenchyma of *** in different degrees,which proved that ROS and ethylene(ET)were positively correlated with the formation of ***,AT and ETH well induced the expression of the ShCTR1 gene,and 1-MCP and DPI inhibited the expression of the ShCTR1 gene to different degrees,which proved that ROS and ET were also positively correlated with the expression of the ShCTR1 ***1 was transferred into tobacco(Nicotiana tabacum L.),then repeat the above *** was shown that positive rate and porosity were lowest and highest after DPI and AT treatments,respectively,it showed a positive correlation,which proved that the ShCTR1 gene was positively regulating the formation of *** of the ShCTR1 gene increased the content of ROS,consequently,the regulation of aerenchyma formation by the ShCTR1 gene is mainly mediated by *** suggested that the formation of aerenchyma in *** was related to hypoxia in the alpine environment,and ShCTR1 interacts with ShRBOH1 to positively regulate the formation of a

关键词： Saussurea inversa aerenchyma ROS gene function

Washed microbiota transplantation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabolomic-based study

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World Journal of Gastroenterology 2024年第11期30卷 1572-1587页

作者： shi-ju Chen Da-Ya Zhang Xia Wu Fa-Ming Zhang Bo-Ta Cui Yi-Hao Huang Zu-Lun Zhang rui Wang Fei-Hu Bai Graduate School Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Microbiota Medicine&Medical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of Gastroenterology The Second Affiliated Hospital of Hainan Medical UniversityHaikou 570216Hainan ProvinceChina

BACKGROUND Fecal microbiota transplantation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transplantation(WMT).Most existing studies have focused on observing the clinical ***,the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome,metatranscriptome,and metabolome-has not yet been *** To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to *** We conducted a prospective,open-label,single-center clinical *** CD patients underwent *** clinical responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabolome profiles were evaluated three months after the *** Seven of the 11 patients(63.6%)showed an optimal clinical response three months *** microbiome diversity significantly increased after WMT,consistent with improved clinical *** of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains,such as Faecalibac-terium prausnitzii,Roseburia intestinalis,and Escherichia *** addition,metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment compared to the ***,levels of vital amino acids that may be associated with disease progression(e.g.,L-glutamic acid,gamma-glutamyl-leucine,and prolyl-glutamine)were reduced after *** WMT demonstrated therapeutic efficacy in CD treatment,likely due to the effective reconstruction of the patient’s ***-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

关键词： Crohn’s disease Clinical trials Fecal microbiota transplant Metagenome Metatranscriptome Metabolome