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n-octyl acrylate is a candidate sex pheromone component involved in courtship in parasitoid wasp Microplitis mediator

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Insect Science 2024年第4期31卷 1200-1210页

作者： Yu-Xiao Tao Shuang Shan Youssef Dewer Shan-Ning Wang Adel Khashaveh rui-jun li Yong-jun Zhang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina College of Plant Protection Agricultural University of HebeiBaodingHebei ProvinceChina Bioassay Research Department Central Agricultural Pesticide LaboratorySabahia Plant Protection Research StationAgricultural Research CenterAlexandriaEgypt Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijingChina

Sex pheromones are considered to play critical roles in partner communication of most parasitic ***,the identification of sex pheromone components remains limited to a few families of parasitoid *** this study,we functionally characterized a candidate sex pheromone component in Microplitis mediator(Hymenoptera:Braconidae),a solitary parasitoid of Noctuidae *** found that the body surface extract from female wasps could significantly stimulate courtship behavior of *** chromatography-electroantennographic detection(GC-EAD)analysis revealed that a candidate semiochemical from extract triggered significant electrophysiological response of antennae of *** performing gas chromatography-mass spectrometer(GC-MS)measurement,GC-EAD active compound was identified as n-octyl acrylate,a candidate sex pheromone component in female *** electroantennogram(EAG)tests,antennae of male wasps showed significantly higher electrophysiological responses to n-octyl acrylate than those of females.Y-tube olfactometer assays indicated that male wasps significantly chose n-octyl acrylate compared with the ***,male wasps showed a remarkable preference for n-octyl acrylate in a simulated field condition behavioral trial;simultaneously,n-octyl acrylate standard could also trigger significant courtship behavior in *** propose that n-octyl acrylate,as a candidate vital sex pheromone component,could be utilized to design behavioral regulators of *** to implement the protection and utilization of natural enemies.

关键词： behavioral assay courtship behavior GC-EAD analysis Microplitis mediator sex pheromone

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HPLC法同时测定知黄抗癌方中7种成分的含量

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中成药 2024年第7期46卷 2153-2156页

作者：芮黎珺杨喜梅王瑶刘梅徐嘉复旦大学附属肿瘤医院药剂科复旦大学上海医学院肿瘤学系上海200032 上海中医药大学中医健康服务协同创新中心上海201203 上海交通大学医学院附属第九人民医院辅助生殖科上海200011 上海中医药大学技术转移中心上海201203

目的建立HPLC法同时测定知黄抗癌方中红景天苷、绿原酸、芒果苷、虎杖苷、盐酸小檗碱、异牡荆素、白藜芦醇的含量。方法分析采用SPOLARC_(18)色谱柱(4.6mm×250mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0mL/min;柱温30℃;检测... 详细信息

目的建立HPLC法同时测定知黄抗癌方中红景天苷、绿原酸、芒果苷、虎杖苷、盐酸小檗碱、异牡荆素、白藜芦醇的含量。方法分析采用SPOLARC_(18)色谱柱(4.6mm×250mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0mL/min;柱温30℃;检测波长275nm。结果7种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率91.32%~106.95%,RSD0.4%~2.9%。结论该方法简便快速,专属性好,可用于知黄抗癌方的质量控制。

关键词：知黄抗癌方化学成分含量测定 HPLC

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Theoretical analysis of the double-differential cross-sections of neutron,proton,deuteron,^(3)He,andαfor the p+^(6) li reaction

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Nuclear Science and Techniques 2024年第3期35卷 181-197页

作者： Fang-Lei Zou Xiao-jun Sun Jing-Shang Zhang Hai-rui Guo Yin-Lu Han rui-rui Xu Xi Tao Ji-Min Wang Xiao-Dong Sun Yuan Tian Tao Ye Yong-li Xu Chun-Tian liang College of Physics Guangxi Normal University541004 GuilinChina Guangxi Key Laboratory of Nuclear Physics and Nuclear Technology 541004 GuilinChina China Institute of Atomic Energy P.O.Box 275(41)102413 BeijingChina Institute of Applied Physics and Computational Mathematics 10094 BeijingChina College of Physics and Electronic Shanxi Datong University037009 DatongChina School of Science Tianjin Chengjian University300384 TianjinChina

Based on the unified Hauser–Feshbach and exciton model,which can describe the particle emission processes between discrete energy levels with energy,angular momentum,and parity conservations,a statistical theory of light nucleus reaction(STLN)is developed to calculate the double-differential cross-sections of the outgoing neutron and light charged particles for the proton-induced^(6) li reaction.A significant difference is observed between the p+^(6) li and p+^(7) li reactions owing to the discrepancies in the energy-level structures of the *** reaction channels,including sequential and simultaneous emission processes,are analyzed in *** the double-differential cross-sections of the outgoing proton as an example,the influence of contaminations(such as^(1) H,^(7)li,^(12)C,and^(16)O)on the target is identified in terms of the kinetic energy of the first emitted *** optical potential parameters of the proton are obtained by fitting the elastic scattering differential *** calculated total double-differential cross-sections of the outgoing proton and deuteron at E_(p)=14 MeV agree well with the experimental data for different outgoing ***,the mixed double differential cross-sections of^(3) He andαare in good agreement with the *** agreement between the measured data and calculated results indicates that the two-body and three-body breakup reactions need to be considered,and the pre-equilibrium reaction mechanism dominates the reaction *** on the STLN model,a PLUNF code for the p+^(6) li reaction is developed to obtain an ENDF-6-formatted file of the double-differential cross-sections of the nucleon and light composite charged particles.

关键词： Statistical theory of light nucleus reaction p+^(6)li reaction light composite charged particle Double-differential cross-sections Two-body breakup Three-body breakup

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Neutrophil-to-lymphocyte ratio compared to N-terminal pro-brain natriuretic peptide as a prognostic marker of adverse events in elderly patients with chronic heart failure

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Journal of Geriatric Cardiology 2017年第2期14卷 127-134页

作者： Wei YAN rui-jun li Qian JIA Yang MU Chun-Lei liU Kun-Lun HE Department of Cardiology Chinese PLA General Hospital Beijing China School of Medicine Nankai University Tianjin China

Background The neutrophil-to-lymphocyte （N/L） ratio has been associated with poor prognosis in patients with heart failure, but it has not been compared with N-terminal pro-brain natriuretic peptide （NT-proBNP） in elderly patients with chronic heart failure （CHF）. We sought to make this comparison. Methods A total of 1355 elderly patients with CHF were analyzed. A multivariate logistic regression model was used to analyze the variables associated with atrial fibrillation （AF）. Cox regression analysis was used to assess the multivariable rela- tionship between the N/L ratio, NT-proBNP level, and subsequent major cardiovascular events （MCE）. Results In the multiple logistic regression analysis, the N/L ratio was demonstrated as a risk factor for AF in elderly patients with CHF [odds ratio （OR）： 1.079, 95% confi- dence interval （CI）： 1.027-1.134, P = 0.003]. The median follow-up period was 18 months. In a multivariable model using tertiles of both variables, the highest tertile of the N/L ratio was significantly associated with MCE [hazard ratio （HR）： 1.407, 95% CI： 1.098-1.802, P = 0.007] compared with the lowest tertile. Similarly, the highest NT-proBNP tertile was also significantly associated with MCE （HR： 1.461, 95% CI： 1.104-1.934, P- 0.008）. Conclusions In elderly patients with CHF, the N/L ratio is one of the important risk factors for AF and it is an inexpensive and readily available marker with similar independent prognostic power to NT-proBNP. The risk of MCE increases 1.407-fold when the N/L ratio is elevated to the highest tertile.

关键词： Atrial fibrillation Chronic heart failure Elderly patients Neutrophil-to-lymphocyte ratio N-terminal pro-brain natriureticpeptide

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Salubrinal protects against tunicamycin and hypoxia induced cardiomyocyte apoptosis via the PERK-eIF2a signaling pathway

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Journal of Geriatric Cardiology 2012年第3期9卷 258-268页

作者： Chun-Lei liu Xin li Guo-liang Hu rui-jun li Yun-Yun He Wu Zhong Song li Kun-Lun He li-li Wang Department of Cardiology Chinese PLA General Hospital28 Fuxing RoadBeijing 100853China Medical School of Nankai University 74 Weijin RoadTianjin 300074China Pharmacy Institute of Military Medical Sciences 27 Taiping RoadBeijing 100850China

Objectives This study examined the protective effect of salubrinal and the mechanism underlying this protection against tunicamycin （TM）- and hypoxia-induced apoptosis in rat cardiomyocytes. Methods Neonatal rat cardiomyocytes were cultured from the ventricles of l-day-old Wistar rats. Cells were exposed to different concentrations of salubrinal （10, 20, and 40 gmol/L） for 30 min followed by TM treatment or hypoxia for 36 h. Apoptosis was measured by a multiparameter HCS （high content screening） apoptosis assay, TUNEL assay and flow cytometry. The phosphorylation of eukaryotic translation initiation factor 2 subunit alpha （eIF2c0 and the expression of cleaved caspase-12 were determined by Western blotting. C/EBP homologous protein （CHOP） was detected by immunocytochemistry. Results HCS, TUNEL assays and flow cytometry showed that salubrinal protected cardiomyocytes against apoptosis induced by TM or hypoxia. Western blotting showed that salubrinal protected cardiomyocytes against apoptosis by inducing eIF2ct phosphorylation and down-regulating the expression of the endoplasmic reticulum stress-mediated apoptotic proteins, CHOP and cleaved caspase-12. Conclusions Our study suggests that salubrinal protects rat cardiomyocytes against TM- or hypoxia-associated apoptosis via a mechanism involving the inhibition of ER stress-mediated apoptosis.

关键词： Endoplasmic reticulum stress Rat cardiomyocytes Apoptosis Salubrinal Cell protection

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Research on high energy efficiency and low bit-width floating-point type data for abnormal object detection of transmission lines

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Global Energy Interconnection 2024年第3期7卷 324-335页

作者： Chen Wang Guozheng Peng rui Song jun Zhang li Yan China Electric Power Research Institute Beijing 100192P.R.China Information&Telecommunications Company State Grid Shandong Electric Power CompanyJinan 250001P.R.China

Achieving a balance between accuracy and efficiency in target detection applications is an important research *** detect abnormal targets on power transmission lines at the power edge,this paper proposes an effective method for reducing the data bit width of the network for floating-point *** performing exponent prealignment and mantissa shifting operations,this method avoids the frequent alignment operations of standard floating-point data,thereby further reducing the exponent and mantissa bit width input into the training *** enables training low-data-bit width models with low hardware-resource consumption while maintaining *** tests were conducted on a dataset of real-world images of abnormal targets on transmission *** results indicate that while maintaining accuracy at a basic level,the proposed method can significantly reduce the data bit width compared with single-precision *** suggests that the proposed method has a marked ability to enhance the real-time detection of abnormal targets in transmission ***,a qualitative analysis indicated that the proposed quantization method is particularly suitable for hardware architectures that integrate storage and computation and exhibit good transferability.

关键词： Power edge Data format Quantification Compute-in-memory

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Unraveling the complex roles of macrophages in obese adipose tissue:an overview

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Frontiers of Medicine 2024年第2期18卷 205-236页

作者： Chang Peng jun Chen rui Wu Haowen Jiang Jia li Hangzhou Institute for Advanced Study University of Chinese Academy of SciencesHangzhou310000China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China University of Chinese Academy of Sciences Beijing100049China Department of Prosthodontics Shanghai Engineering Research Center of Advanced Dental Technology and MaterialsShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyNational Clinical Research Center for Oral DiseasesShanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineShanghai200011China

Macrophages,a heterogeneous population of innate immune cells,exhibit remarkable plasticity and play pivotal roles in coordinating immune responses and maintaining tissue homeostasis within the context of metabolic *** activation of inflammatory macrophages in obese adipose tissue leads to detrimental effects,inducing insulin resistance through increased inflammation,impaired thermogenesis,and adipose tissue ***,adipose tissue macrophages also play a beneficial role in maintaining adipose tissue homeostasis by regulating angiogenesis,facilitating the clearance of dead adipocytes,and promoting mitochondrial *** the heterogeneity of macrophages in obese adipose tissue is crucial for unraveling the pathogenesis of obesity and holds significant potential for targeted therapeutic ***,the dual effects and some potential regulatory mechanisms of macrophages in adipose tissue have been elucidated using single-cell *** this review,we present a comprehensive overview of the intricate activation mechanisms and diverse functions of macrophages in adipose tissue during obesity,as well as explore the potential of drug delivery systems targeting macrophages,aiming to enhance the understanding of current regulatory mechanisms that may be potentially targeted for treating obesity or metabolic diseases.

关键词： obesity inflammation adipose tissue macrophages adipose tissue homeostasis

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CO_(2) hydrogenation to methanol over the copper promoted In_(2)O_(3) catalyst

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Journal of Energy Chemistry 2024年第6期93卷 135-145,I0004页

作者： rui Zou Chenyang Shen Kaihang Sun Xinbin Ma Zhuoshi li Maoshuai li Chang-jun liu School of Chemical Engineering and Technology Tianjin UniversityTianjin 300350China Collaborative Innovation Center of Chemical Science&Engineering Tianjin UniversityTianjin 300072China

The metal promoted In_(2)O_(3) catalysts for CO_(2) hydrogenation to methanol have attracted wide attention because of their high activity with high methanol ***,there was still no experimental confirmation if copper could be a good promoter for In_(2)O_(3).Herein,the Cu promoted In_(2)O_(3) catalyst was prepared using a deposition-precipitation *** prepared Cu/In_(2)O_(3) catalyst shows significantly higher CO_(2) conversion and space time yield(STY)of methanol,compared to the un-promoted In_(2)O_(3) *** loading of Cu facilitates the activation of both H_(2) and CO_(2) with the interface between the Cu cluster and defective In_(2)O_(3) as the active *** Cu/In_(2)O_(3) catalyst takes the CO hydrogenation pathway for methanol synthesis from CO_(2) *** exhibits a unique size effect on the CO *** temperatures below 250℃,CO adsorption on Cu/In_(2)O_(3) is stronger than that on In_(2)O_(3),causing higher methanol *** increasing temperatu res,the Cu catalyst aggregates,which leads to the formation of weak CO adsorption site and causes a decrease in the methanol *** with other metal promoted In_(2)O_(3) catalysts,it can be concluded that the catalyst with stronger CO adsorption possesses higher methanol selectivity.

关键词： CO_(2)hydrogenation Methanol Cu In_(2)O_(3) CO Selectivity DFT

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Research on a Monte Carlo global variance reduction method based on an automatic importance sampling method

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Nuclear Science and Techniques 2024年第5期35卷 200-215页

作者： Yi-Sheng Hao Zhen Wu Shen-Shen Gao rui Qiu Hui Zhang jun-li li Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging of Ministry of Education Beijing 100084China Nuctech Company limited Beijing 100084China

Global variance reduction is a bottleneck in Monte Carlo shielding *** global variance reduction problem requires that the statistical error of the entire space is *** study proposed a grid-AIS method for the global variance reduction problem based on the AIS method,which was implemented in the Monte Carlo program *** proposed method was validated using the VENUS-Ⅲ international benchmark problem and a self-shielding calculation *** results from the VENUS-Ⅲ benchmark problem showed that the grid-AIS method achieved a significant reduction in the variance of the statistical errors of the MESH grids,decreasing from 1.08×10^(-2) to 3.84×10^(-3),representing a 64.00% *** demonstrates that the grid-AIS method is effective in addressing global *** results of the selfshielding calculation demonstrate that the grid-AIS method produced accurate computational ***,the grid-AIS method exhibited a computational efficiency approximately one order of magnitude higher than that of the AIS method and approximately two orders of magnitude higher than that of the conventional Monte Carlo method.

关键词： Monte Carlo Global variance reduction Reactor shielding Automatic importance sampling

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Characterization and target gene analysis of microRNAs in the antennae of the parasitoid wasp Microplitis mediator

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Insect Science 2021年第4期28卷 1033-1048页

作者： Shuang Shan Shan-Ning Wang Xuan Song Adel Khashaveh Zi-Yun Lu Khalid Hussain Dhiloo rui-jun li Xi-Wu Gao Yong-jun Zhang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina College of Plant Protection China Agricultural UniversityBeijingChina Institute of Plant and Environment Protection Beijing Academy of Agricultural and Forestry SciencesBeijingChina College of Plant Protection Agricultural University of HebeiBaodingHebeiChina IPM Center of Hebei Province Key Laboratory of Integrated Pest Management on Crops in Northern Region of North ChinaMinistry of AgriculturePlant Protection InstituteHebei Academy of Agricultural and Forestry SciencesBaodingHebeiChina Department of Entomology Faculty of Crop ProtectionSindh Agriculture UniversityTandojamPakistan

MicroRNAs(miRNAs),a class of non-coding single-strand RNA molecules encoded by endogenous genes,are about 21–24 nucleotides long and are involved in the post-transcriptional regulation of gene expression in plants and ***,the types and quantities of miRNAs in the different tissues of an organism are diverse,and these divergences may be related to their specific *** we have identified 296 known miRNAs and 46 novel miRNAs in the antennae of the parasitoid wasp Microplitis mediator by high-throughput ***-three miRNAs were predicted to target olfactory-associated genes,including odorant binding proteins(OBPs),chemosensory proteins,odorant receptors(ORs),ionotropic receptors(IRs)and gustatory *** these,17 miRNAs were significantly highly expressed in the antennae,four miRNAs were highly expressed both in the antennae and head or wings,while the remaining 12 miRNAs were mainly expressed in the head,thorax,abdomen,legs and ***,miR-9a-5p and miR-2525-3p were highly expressed in male antennae,whereas miR-1000-5p and novel-miR-13 were enriched in female *** 17 miRNAs highly expressed in antennae are likely to be associated with olfaction,and were predicted to target one OBP(targeted by miR-3751-3p),one IR(targeted by miR-7-5p)and 14 ORs(targeted by 15 miRNAs including miR-6-3p,miR-9a-5p,miR-9b-5p,miR-29-5p,miR-71-5p,miR-275-3p,miR-1000-5p,miR-1000-3p,miR-2525-3p,miR-6012-3p,miR-9719-3p,novel-miR-10,novel-miR-13,novel-miR-14 and novel-miR-28).These candidate olfactory-associated miRNAs are all likely to be involved in chemoreception through the regulation of chemosensory gene expression in the antennae of ***.

关键词： expression profile miRNA Microplitis mediator,olfactory-associated gene target gene

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