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Postoperative subacute static progressive stretch does not increase the risk of distal lower limb venous thromboembolism

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Chinese Journal of Traumatology 2023年第3期26卷 178-182页

作者： Jun-Kun Zhu feng-feng Wu rui-feng yang Fen-Fen Xu Ya-Li Lin Miao-Fang Ye Orthopedics Rehabilitation Department Lishui Central HospitalLishuiZhejiang Province323020China Rehabilitation Department Huzhou Central HospitalHangzhou310020China Orthopedics and Trauma Department Lishui Central HospitalLishuiZhejiang Province323020China

Purpose:Static progressive stretch(SPS)can be applied to treat chronic joint stiffness.However,the impacts of subacute application of SPS to the distal lower limbs,where deep vein thrombosis(DVT)is common,on venous thromboembolism remain unclear.This study aims to explore the risk of venous thromboembolism events following subacute application of SPS.Methods:A retrospective cohort study was conducted on patients diagnosed with DVT following a lower extremity orthopedic surgery before being transferred to the rehabilitation ward from May 2017 to May 2022.Patients with unilateral lower limb comminuted para-articular fractures,transferred to rehabilitation ward for further treatment within 3 weeks after operation,followed up more than 12 weeks since initial manual physiotherapy,and diagnosed DVT by ultrasound before rehabilitation course were included in the study.Patients with polytrauma,without evidence of previous peripheral vascular disease or incompetence,had medication for thrombosis treatment or prophylaxis before the operation,detected with paralysis due to nervous system impairment,infected after operation during the regime,or with acute progression of DVT were excluded.The included patients were randomized to the standard physiotherapy and the SPS integrated groups for observation.Associated DVT and pulmonary embolism data were collected during the physiotherapy course to compare the groups.SSPS 28.0 and GraphPad Prism 9 were used for data processing.Ap<0.05 was set significant difference.Results:In total of 154 patients with DVT participating in this study,75 of them were treated with additional SPS for postoperative rehabilitation.The participants in the SPS group showed improved range of motion(12.3°±6.7°).However,in the SPS group,there was no difference in thrombosis volume between the start and termination(p=0.106,p=0.787,respectively),although difference was seen intra-therapy(p<0.001).Contingency analysis revealed the pulmonary embolism incidence(OR=0.703)in the SPS group compared to the mean physio

关键词： Trauma Deep vein thrombosis Rehabilitation Musculoskeletal manipulations Physical therapy modalities

HBcrAg Identifies Patients Failing to Achieve HBeAg Seroconversion Treated with Pegylated Interferon Alfa-2b

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Chinese Medical Journal 2016年第18期129卷 2212-2219页

作者： Hui Ma rui-feng yang Xiao-He Li Qian Jin Lai Wei Department of Hepatology Peking University People's Hospital Beijing 100044 China Peking University Hepatology Institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University People's Hospital Beijing 100044 China

Background： We aimed to evaluate the usefulness of serum hepatitis B virus core-related antigens （HBcrAg） for predicting hepatitis B e antigen （HBeAg） seroconversion in HBeAg-positive chronic hepatitis B patients treated with conventional interferon （IFN） alfh-2b or pegylated IFN. Methods： Fifty-eight patients were enrolled： 29 for the training group and 29 for the validating group. HBcrAg was measured at baseline, week 12, end of the treatment, and 12- and 24-week follow-ups. Sixteen patients in the training group were enrolled in the long-term follow-up （LTFU）, during which time the dynamics of the HBcrAg was monitored. Results： The serum HBcrAg level gradually declined during treatment among the HBeAg seroconversion patients of the training group （from baseline, week 12, end of the treatment, 12-week follow-up to 24-week follow-up were II0,245 kU/ml, 3760 kU/ ml, 7410 kU/ml, 715 kU/ml, 200 kU/ml, respectively）. HBcrAg 〈19,565 kU/ml at week 24, HBcrAg 〈34,225 kU/ml at 12-week follow-up, and HBcrAg decrease 〉0.565 log10 kU/ml from the baseline to the end of treatment （EOT） had negative predictive values （NPVs） of 100% for HBeAg seroconversion at the end of follow-up, whereas the positive predictive values （PPVs） were 30.77%, 26.67%, and 25.00%, respectively. The patients with HBeAg seroconversion at the end of 24-week follow-up remained in seroconversion during the LTFU, during which time their serum HBcrAg levels steadily declined or even became undetectable, ranging from 0 to 2.1 kU/ml. Conclusions： Effective antiviral treatment can decrease HBcrAg levels in the serum. The NPVs of HBcrAg for predicting HBeAg seroconversion at 24-week follow-up was 100%, but the PPVs were not satisfactory （all 〈31%）. The serum HBcrAg levels of the patients with HBeAg seroconversion at the end of the 24-week follow-up steadily declined or even became undetectable during the LTFU.

关键词： Chronic Hepatitis B HBeAg HBcrAg Pegylated Interferon Alfa-2b

Impact of hepatitis C virus genotype 3 on liver disease progression in a Chinese national cohort

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Chinese Medical Journal 2020年第3期133卷 253-261页

作者： Nan Wu Hui-Ying Rao Wei-Bo yang Zhi-Liang Gao rui-feng yang Ran Fei Ying-Hui Gao Qian Jin Lai Wei Peking University People’s Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseaseBeijing 100044China Department of Infectious Diseases The First Affiliated Hospital of Kunming Medical CollegeKunmingYunnan 650032China Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouGuangdong 510630China Hepatopancreatobiliary Center Beijing Tsinghua Changgung HospitalInstitute for Precision MedicineTsinghua UniversityBeijing 102218China

Background:Hepatitis C virus(HCV)genotype 3,particularly subtype 3b,is increasing in prevalence and distribution in China.This study evaluated the prevalence,regional distribution,clinical characteristics,host factors,treatment outcomes,and disease progression of patients with HCV genotype 3 in China.Methods:A 5-year follow-up was preceded by a cross-sectional study.Treatment choices were at the discretion of treating physicians.Estimated infection time to overall-disease-progression(defined by≥1 of:newly diagnosed cirrhosis;cirrhosis at baseline,Child-Turcotte-Pugh score increased 2 points or more;progression from compensated cirrhosis to decompensated cirrhosis;hepatocellular carcinoma;liver transplantation;or death)was calculated using the Kaplan-Meier method.Cox regression analyses were conducted to evaluate the risk factors for disease progression.Results:The cross-sectional study enrolled 997 patients,including 91 with HCV genotype 3 infection.Among them,subtype 3b(57.1%)was more dominant than subtype 3a(38.5%).Five hundred and twelve patients were included into the follow-up phase.Among patients analyzed for estimated infection time to overall-disease-progression,52/304(17.1%)patients with HCV genotype 1 and 4/41(9.8%)with HCV genotype 3(4/26 with genotype 3b,0/13 with genotype 3a,and 0/2 with undefined subtype of genotype 3)experienced overall-disease-progression.Patients with HCV genotype 3 were younger than those with genotype 1(mean age:39.5±8.7 vs.46.9±13.6 years)and demonstrated more rapid disease progression(mean estimated infection time to overall-disease-progression 27.1 vs.35.6 years).Conclusions:HCV genotype 3,specifically subtype 3b,is associated with more rapid progression of liver disease.Further analysis to compare HCV subtype 3a and 3b is needed in high prevalence regions.

关键词： Hepatitis C virus genotype 3 Chronic hepatitis C Disease progression

Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation

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Chinese Medical Sciences Journal 2013年第2期28卷 82-87页

作者： rui-feng yang Guo-wei Zhao Shu-ting Liang Hou-zao Chen De-pei Liu National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College

Objective To investigate the role of lysine-specific demethylase 1 (LSD1) in the process of THP-1 monocyte-to-macrophage differentiation.Methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting were performed to analyze the expression of LSD1 and interleukin-6 (IL-6) in THP-1 monocytes and THP-1-derived macrophages.Chromatin immunoprecipitation (ChIP) assay was applied to detect the occupancy of LSD1 and H3K4 methylation at IL-6 promoter during THP-1 monocyte-to-macrophage differentiation.IL-6 mRNA level and H3K4 methylation at IL-6 promoter were analyzed using qRT-PCR and ChIP assay in LSD1-knockdown THP-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 0,4,8,12,and 24 hours.Fluorescence activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP-1 monocytes.Results The expression of LSD1 reduced during THP-1 monocyte-to-macrophage differentiation (P<0.01).LSD1 occupancy decreased and H3K4 methylation increased at IL-6 promoter during the differentiation.With knockdown of LSD1,H3K4 methylation at IL-6 promoter was found increased after TPA treatment at different times points (all P<0.05,except 24 hours).The percentage of macrophages increased significantly in the THP-1 cells with LSD1 knockdown (P<0.05).Conclusions LSD1 is repressed during the monocyte-to-macrophage differentiation of THP-1 cells.Suppression of LSD1-mediated H3K4 demethylation may be required for THP-1 monocyte-to-macrophage differentiation.

关键词：单核细胞巨噬细胞甲基化酶细胞分化赖氨酸异性定量RT-PCR Western印迹

NF-E2:a Novel Regulator of Alpha-hemoglobin Stabilizing Protein Gene Expression

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Chinese Medical Sciences Journal 2010年第4期25卷 193-198页

作者： Guo-wei Zhao rui-feng yang Xiang Lu Mitchell J. Weiss De-pei Liu Chih-chuan Liang National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100005 China Children＇s Hospital of Philadelphia Philadelphia 19104 PA USA

Objective To investigate whether α-hemoglobin stabilizing protein (AHSP), the α-globin-specific molecular chaperone, is regulated by erythroid transcription factor NF-E2. Methods We established the stable cell line with NF-E2p45 (the larger subunit of NF-E2) short hairpin RNA to silence its expression. Western blot, real-time polymerase chain reaction, and chromatin immunoprecipitation (ChIP) analysis were performed to detect the expression of AHSP, the histone modifications at AHSP gene locus, and the binding of GATA-1 at the AHSP promoter with NF-E2p45 deficiency. ChIP was also carried out in dimethyl sulfoxide (DMSO)-induced DS19 cells and estrogen-induced G1E-ER4 cells to examine NF-E2 binding to the AHSP gene locus and its changes during cell erythroid differentiation. Finally, luciferase assay was applied in HeLa cells transfected with AHSP promoter fragments to examine AHSP promoter activity in the presence of exogenous NF-E2p45. Results We found that AHSP expression was highly dependent on NF-E2p45. NF-E2 bound to the regions across AHSP gene locus in vivo, and the transcription of AHSP was transactivated by exogenous NF-E2p45. In addition, we observed the decrease of H3K4 trimethylation and GATA-1 occupancy at the AHSP gene locus in NF-E2p45-deficient cells. Restoration of GATA-1 in G1E-ER4 cells in turn led to increased DNA binding of NF-E2p45. Conclusion NF-E2 may play an important role in AHSP gene regulation, providing new insights into the molecular mechanisms underlying the erythroid-specific expression of AHSP as well as new possibilities for β-thalassemia treatment.

关键词：血红蛋白 E2 基因表达稳定染色质免疫沉淀基因位点转录因子聚合酶链反应

脐带间充质干细胞增强无精子症模型睾丸特异性基因表达

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Asian Journal of Andrology 2014年第5期16卷 698-704,I0007页

作者： rui-feng yang Tai-Hua Liu Kai Zhao Cheng-Liang Xiong Center of Reproductive Medicine Family Planning Research Institute Tengji Medical College Huazhong University of Science and Technology Wuhan Tongji Reproductive Medicine Hospital Wuhan China

在生殖男科领域，探索人脐带间充质干细胞（HUCMSCs）对无精子症的治疗作用。HUCMSCs具有潜在的免疫抑制功能，并能够分泌多种细胞因子和生长因子，因此具有潜在的临床应用价值。作为探索，我们移植HUCMSCs进入无精子症小鼠睾丸间质，... 详细信息

在生殖男科领域，探索人脐带间充质干细胞（HUCMSCs）对无精子症的治疗作用。HUCMSCs具有潜在的免疫抑制功能，并能够分泌多种细胞因子和生长因子，因此具有潜在的临床应用价值。作为探索，我们移植HUCMSCs进入无精子症小鼠睾丸间质，检测是否能够促进精子发生过程。从不同来源的脐带中分离HUCMSCs，移植进入白消安处理的小鼠睾丸间质中，采用注射生理盐水和HEK293细胞作为对照，对侧睾丸不注射。三周之后，RT-PCR检测10个生殖细胞特异性表达的基因，并检N3个特异性蛋白的表达。结果表明，注射人脐带间充质干细胞之后的表达明显高于对照组，证实生殖细胞特异性基因的表达上调，从而表明HUCMSCs对睾丸生精功能的恢复具有促进作用，为治疗无精子症探索一条新的途径。

关键词：无精症生殖细胞特异性基因人脐带间充质干细胞男性不育

Thermal-force control ability under variation of electromagnetic stick structure parameters in RPECT

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Journal of Iron and Steel Research(International) 2024年第1期31卷 224-236页

作者： Ting-song yang Wen-quan Sun feng-shan Du An-rui He Quan yang National Engineering Research Center of Advanced Rolling and Intelligent Manufacturing University of Science and Technology BeijingBeijing100083China Institute of Engineering and Technology University of Science and Technology BeijingBeijing100083China College of Mechanical Engineering Yanshan UniversityQinhuangdao066004HebeiChina

Thermal-force driving of roll profile electromagnetic control technology(RPECT),which can be used to adjust the roll profile,can be affected by the sequential temperature rise between the electromagnetic stick(ES)and electromagnetic control roll.Due to the limited space of ES and induction coil,the cross-sectional area of induction coil can be inevitably affected by changing the size of the ES induction zone,which can further change the energy input under the same electromagnetic parameters,the temperature rising effect and the bulging ability.To investigate this phenomenon,the effects of the radius of the induction zone on the thermal-force contribution ratio,the heating ability of ES and the temperature distribution were analyzed through an electromagnetic-thermal-structural finite element model.To ensure that the results are applicable to RPECT,the thermal energy conversion ability and thermal-force roll crown control ability under different lengths of the induction zone were analyzed.It was found that whether the current density regulation mode or the current frequency regulation mode is adopted,the cases with 20 or 25 mm radius of the induction zone have the great thermal energy conversion ability and the good thermal-force roll crown control ability.The reasonable adjustment of the length of the induction zone can reduce the radius required for the maximum energy efficiency regulation.Combined with the results of the simulation analysis,the optimization of ES based on the control ability maximization requirement is achieved,which provides the base for the design and configuration of ES in RPECT.

关键词： Roll profile electromagnetic control technology Electromagnetic stick Electromagnetic control roll Induction area Thermal-force driving

Prediction of Lubricant Physicochemical Properties Based on Gaussian Copula Data Expansion

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China Petroleum Processing & Petrochemical Technology 2024年第1期26卷 161-174页

作者： feng Xin yang rui Xie Peiyuan Xia Yanqiu School of Energy Power and Mechanical Engineering North China Electric Power UniversityBeijing 102206China

The composition of base oils affects the performance of lubricants made from them.This paper proposes a hybrid model based on gradient-boosted decision tree(GBDT)to analyze the effect of different ratios of KN4010,PAO40,and PriEco3000 component in a composite base oil system on the performance of lubricants.The study was conducted under small laboratory sample conditions,and a data expansion method using the Gaussian Copula function was proposed to improve the prediction ability of the hybrid model.The study also compared four optimization algorithms,sticky mushroom algorithm(SMA),genetic algorithm(GA),whale optimization algorithm(WOA),and seagull optimization algorithm(SOA),to predict the kinematic viscosity at 40℃,kinematic viscosity at 100℃,viscosity index,and oxidation induction time performance of the lubricant.The results showed that the Gaussian Copula function data expansion method improved the prediction ability of the hybrid model in the case of small samples.The SOA-GBDT hybrid model had the fastest convergence speed for the samples and the best prediction effect,with determination coefficients(R^(2))for the four indicators of lubricants reaching 0.98,0.99,0.96 and 0.96,respectively.Thus,this model can significantly reduce the model’s prediction error and has good prediction ability.

关键词： base oil data augmentation machine learning performance prediction seagull algorithm

On the Performances of Estimating Stellar Atmospheric Parameters from CSST Broad-band Photometry

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Research in Astronomy and Astrophysics 2024年第4期24卷 161-173页

作者： rui-feng Shi yang Huang Xin-Yi Li Hua-Wei Zhang School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China South-Western Institute for Astronomy Research Yunnan UniversityKunming 650500China CAS Key Lab of Optical Astronomy Key Laboratory of Space Astronomy and TechnologyNational Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China College of Physics and Electronic Engineering Qilu Normal UniversityJinan 250200China Department of Astronomy School of PhysicsPeking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China

Deriving atmospheric parameters of a large sample of stars is of vital importance to understand the formation and evolution of the Milky Way.Photometric surveys,especially those with near-ultraviolet filters,can offer accurate measurements of stellar parameters,with the precision comparable to that from low/medium resolution spectroscopy.In this study,we explore the capability of measuring stellar atmospheric parameters from Chinese Space Station Telescope(CSST)broad-band photometry(particularly in the near-ultraviolet bands),based on synthetic colors derived from model spectra.We find that colors from the optical and near-ultraviolet filter systems adopted by CSST show significant sensitivities to the stellar atmospheric parameters,especially the metallicity.According to our mock data tests,the precision of the photometric metallicity is quite high,with typical values of0.17 and 0.20 dex for dwarf and giant stars,respectively.The precision of the effective temperature estimated from broad-band colors are within 50 K.

关键词： methods:data analysis stars:abundances surveys