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Implantable Polyurethane Scaffolds Loading with PEG-Paclitaxel Conjugates for the Treatment of Glioblastoma Multiforme

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Chinese Journal of Polymer Science 2022年第5期40卷 491-503页

作者： He-cheng Wu Yuan Feng Xing-ying Song Chun-Yang Song Jin-lin chen Yan-Chao wang Xue-ling He rui-Chao liang Jie-Hua li Hong Tan College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China Department of Neurosurgery West China HospitalSichuan UniversityChengdu 610041China Laboratory Animal Center of Sichuan University Chengdu 610041China

Improvement of the treatment for Glioblastoma multiforme(GBM)especially the development of in situ controllable drug release is still a major concern.In this study,we developed waterborne biodegradable polyurethane(WBPU)scaffolds incorporated with redox-sensitive and RGD-decorated paclitaxel(PTX)polymer-drug conjugates(PDCs)for targeted GBM therapy in situ.The drug scaffolds could be implanted at residual GBM site post-operation.Dual-targeting PTX-PDCs were obtained through step-by-step conjugation of disulfide linked PTX,poly(ethylene glycol)(PEG),and arginine-glycine-aspartic acid(RGD).The RGD-modified PTX-PDCs were spherical nanoparticles(NPs)that would be released from scaffolds and identified GBM cells actively.Internalized redox-sensitive PTX-PDCs would be decomposed and release PTX inside GBM cells under the circumstances of glutathione(GSH).The release profiles of PTX from the scaffolds with/without GSH were investigated.In vitro cytotoxicity assay revealed that the dual-targeting PTX-PDCs from scaffolds could specifically kill GBM cells and protect normal cells,suggesting that dual-targeting PTX-PDC-loaded scaffolds may have the potential to repair tumor-induced brain injury.In vivo anti-recurrence assay indicated that the PTX-PDC-scaffolds could deliver PTX-PDCs to the GBM cells followed by inhibiting tumor growth and inducing apoptosis.In general,the PTX prodrug-loaded devices exhibited significant anti-GBM effects and normal tissue protection simultaneously,indicating that the WBPU scaffolds incorporated with dual-targeting PTX-PDCs may be a promising strategy for local therapy of GBM.

关键词： GBM PDCs WBPU Scaffolds Controlled release

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Circulating exosome-like vesicles of humans with nondiabetic obesity impaired isletβ-cell proliferation,which was associated with decreased Omentin-1 protein cargo

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Genes & Diseases 2022年第4期9卷 1099-1113页

作者： Qian Ge Xinxin Xie Xiangjun chen Rongfeng Huang cheng-Xue rui Qianna Zhen Renzhi Hu min Wu Xiaoqiu Xiao Xi li Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China The Biology Science Institutes Chongqing Medical UniversityChongqing 400016PR China The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China de Duve Institute Catholic University of LouvainBrussels 1200Belgium

The regulation ofβ-cell mass in the status of nondiabetic obesity remains not well understood.We aimed to investigate the role of circulating exosome-like vesicles(ELVs)isolated from humans with simple obesity in the regulation of isletβ-cell mass.Between June 2017 and July 2019,81 subjects with simple obesity and 102 healthy volunteers with normal weight were recruited.ELVs were isolated by ultra-centrifugation.The proliferations ofβ-cells and islets were measured by 5-ethynl-2′-deoxyuridine(EdU).Protein components in ELVs were identified by Quantitative Proteomic Analysis and verified by Western blot and Elisa.The role of specific exosomal protein was analyzed by gain-of-function approach in ELVs released by 3T3-L1 preadipocytes.Circulating ELVs from subjects with simple obesity inhibitedβ-cell proliferation in vitro without affecting its apoptosis,secretion,and inflammation.The protein levels of Rictor and Omentin-1 were downregulated in circulating ELVs from subjects with simple obesity and associated with the obesity-linked pathologic conditions.The ELV-carried Omentin-1 and Omentin-1 protein per se were validated to increaseβ-cell proliferation and activate Akt signaling pathway.Moreover,Omentin-1 in ELVs was downregulated by insulin.The circulating ELVs may act as a negative regulator forβ-cell mass in nondiabetic obesity through inhibitingβ-cell proliferation.This effect was associated with downregulated Omentin-1 protein in ELVs.This newly identified ELV-carried protein could be a mediator linking insulin resistance to impairedβ-cell proliferation and a new potential target for increasingβ-cell mass in obesity and T2DM.

关键词： β-Cell Exosome Obesity Proliferation Type 2 diabetes mellitus

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The application of internal suspension technique in retroperitoneal robot-assisted laparoscopic partial nephrectomy with a new robotic system KangDuo Surgical Robot-01:Initial experience

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Asian Journal of Urology 2023年第4期10卷 482-487页

作者： Silu chen Shubo Fan Hua Guan Kunlin Yang Zhihua li Shengwei Xiong Xiang wang Zhenyu li cheng Shen liqun zhou Xuesong li Department of Urology Peking University First HospitalInstitute of UrologyPeking UniversityNational Urological Cancer CenterXicheng DistrictBeijingChina

Objective To assess the feasibility of internal suspension technique in retroperitoneal robot-assisted laparoscopic partial nephrectomy(rRAPN)with a new robotic platform called KangDuo Surgical Robot-01(KD-SR-01)system(Suzhou KangDuo Robot Co.,Ltd.,Suzhou,China)and discuss its surgical technique.Methods A 44-year-old male patient was admitted with a 2.5 cm tumor on dorsolateral upper pole of the left kidney.The R.E.N.A.L.nephrometry score of this patient was 4x.This patient underwent rRAPN with KD-SR-01.The perinephric fat between the tumor and Gerota's fascia was preserved,which was used for internal suspension traction during tumor resection.Postoperative follow-up data were collected.Results The surgery was successfully carried out with a duration of 127 min,in which the docking time was 6 min 25 s and console time was 60 min.The warm ischemia time was 19 min 53 s,and the estimated blood loss was 0 mL.The pathological histology showed a pathological tumor stage 1a clear cell renal cell carcinoma,with a negative surgical margin.The World Health Organization/International Society of Urological Pathology(WHO/ISUP)grade of this patient was Grade 2.No recurrence was observed during the 6-month follow-up.Conclusion Internal suspension in rRAPN is feasible and effective with use of the new robotic system KD-SR-01.

关键词： KangDuo Surgical Robot-01 Internal suspension Partial nephrectomy Retroperitoneal approach

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Tenofovir alafenamide significantly increased serum lipid levels compared with entecavir therapy in chronic hepatitis B virus patients

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世界肝病学杂志:英文版(电子版) 2023年第8期15卷 964-972页

作者： rui-min Lai Shan lin Miao-Miao wang Na li Jia-Hui zhou Xiao-Yu lin Tian-Bin chen Yue-Yong Zhu Qi Zheng Department of Hepatology Hepatology Research InstituteThe First Affiliated HospitalFujian Medical UniversityFujian Clinical Research Center for Hepatopathy and Intestinal DiseasesFuzhou 350005Fujian ProvinceChina Department of Hepatology National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhou 350212Fujian ProvinceChina Department of Endocrinology The 910th Hospital of The Joint Service Support ForceQuanzhou 362000Fujian ProvinceChina Department of Laboratory Medicine The First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina

BACKGROUND Tenofovir alafenamide(TAF)has a serum lipid-raising effect in patients with HIV;however,its effect on serum lipids and nonalcoholic fatty liver disease(NAFLD)risk in patients with chronic hepatitis B(CHB)is unclear.AIM To compare the effects of TAF and entecavir(ETV)on serum lipid levels in patients with CHB.METHODS In this retrospective cohort study,the data including the clinical features,serum lipids,and metabolic factors of patients with CHB at baseline and approximately 1 year after TAF or ETV treatment were collected and analyzed.We used propensity score-matched models to assess the effects on high-density lipoprotein,lowdensity lipoprotein,triglycerides,and total cholesterol(TCHO).RESULTS A total of 336 patients(75.60%male)were included;63.69%received TAF and 36.31%received ETV.Compared with the ETV group,the TAF group had significantly higher TCHO levels after treatment(4.67±0.90 vs 4.36±1.05,P=0.006).In a propensity score-matched model for body mass index,age,sex,smoking,drinking,presence of comorbidities such as NAFLD,cirrhosis,diabetes mellitus,and hypertension,TAF-treated patients had significantly increased TCHO levels compared to that at baseline(P=0.019).There was no difference for the ETV group.Body mass index,sex,hypertension,baseline TCHO,and creatine kinase-MB isoenzyme levels were significantly associated with elevated TCHO levels in logistic regression analysis.However,1-year TAF treatment did not increase the incidence of NAFLD.CONCLUSION A greater increase in TCHO was observed in patients with CHB receiving TAF compared to those receiving ETV.However,TAF-induced dyslipidemia did not increase the incidence of NAFLD.

关键词： Tenofovir alafenamide Entecavir Hepatitis B virus Serum lipid Metabolic factor

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采用指示剂氨基酸氧化法评估老年人5年间蛋白质需要量的变化

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卫生研究 2024年第2期53卷 282-287页

作者：吴文璇马辉卢佳希聂淑慧杨云淇陈凤格张艳红王睿李敏中国疾病预防控制中心营养与健康所国家卫生健康委微量元素与营养重点实验室北京100050 石家庄市疾病预防控制中心石家庄050012 正定县疾病预防控制中心石家庄050800

目的评估老年人5年间蛋白质需要量的变化情况。方法在2017年14名老年人蛋白质需要量研究的前期基础上,纳入其中4名老年人(70~80岁)作为研究对象,采用指示剂氨基酸氧化法再次评估老年人蛋白质需要量。设7个蛋白质水平,分别为0.1、0.3、0.... 详细信息

目的评估老年人5年间蛋白质需要量的变化情况。方法在2017年14名老年人蛋白质需要量研究的前期基础上,纳入其中4名老年人(70~80岁)作为研究对象,采用指示剂氨基酸氧化法再次评估老年人蛋白质需要量。设7个蛋白质水平,分别为0.1、0.3、0.6、0.9、1.2、1.5和1.8 g/(kg·d)。每个蛋白质水平前2天均给予维持膳食。第3天进行稳定同位素研究,在氨基酸配比膳食的基础上,采用L-13C-苯丙氨酸作为指示剂,伴随氨基酸配比膳食口服摄入体内,当L-13C-苯丙氨酸的代谢在体内达到稳态时,收集呼出气、尿液样本。通过测定样本中标记氨基酸动力学参数,构建待测蛋白质摄入量和标记氨基酸氧化率的非线性混合效应模型,曲线拐点对应的蛋白质摄入量即为该研究人群的蛋白质平均需要量。结果根据4名老年人不同蛋白水平下呼气中^(13)CO_(2)的产生率可以获得老年人蛋白质平均需要量为1.05(95%CI 0.51~1.60)g/(kg·d)。采用蛋白质平均需要量推算推荐摄入量的变异系数进行推算后获得蛋白质推荐摄入量为1.31(95%CI 0.64~2.00)g/(kg·d)。结论老年人的蛋白质需要量在5年间有所提高,肌少症可能是导致老年人蛋白质需要量增加的主要原因。

关键词：蛋白质需要量老年人指示剂氨基酸氧化

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不同产地木瓜中齐墩果酸和熊果酸的含量测定

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食品安全导刊 2024年第10期 80-83页

作者：冉艳瑞张丽王灿陈艳王蕊昆明市食品药品检验所云南昆明650000

目的:测定不同产地木瓜中齐墩果酸和熊果酸的含量。方法:以云南保山、大理两产地的木瓜为试验材料,通过正交试验对提取方法进行优化,采用高效液相色谱法测定木瓜中齐墩果酸和熊果酸含量。结果:当提取试剂为甲醇、浸泡时间为1 h、超声时... 详细信息

目的:测定不同产地木瓜中齐墩果酸和熊果酸的含量。方法:以云南保山、大理两产地的木瓜为试验材料,通过正交试验对提取方法进行优化,采用高效液相色谱法测定木瓜中齐墩果酸和熊果酸含量。结果:当提取试剂为甲醇、浸泡时间为1 h、超声时间为30 min时,提取效果最佳。结论:不同产地木瓜中齐墩果酸和熊果酸的含量有差异,为木瓜中齐墩果酸和熊果酸的含量测定提供理论依据。

关键词：木瓜高效液相色谱法(HPLC) 齐墩果酸熊果酸含量测定

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Genome-wide identification,evolutionary selection,and genetic variation of DNA methylation-related genes in Brassica rapa and Brassica oleracea

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Journal of Integrative Agriculture 2022年第6期21卷 1620-1632页

作者： AN Feng ZHANG Kang ZHANG ling-kui li Xing chen Shu-min wang Hua-sen cheng Feng Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Mountainous Areas College of Horticulture ScienceZhejiang A&F UniversityHangzhou 311300P.R.China Institute of Vegetables and Flowers Chinese Academy of Agricul tural SciencesBeijing 100081P.R.China

DNA methylation plays an important role in plant growth and development,and in regulating the activity of transposable elements(TEs).Research on DNA methylation-related(DMR)genes has been reported in Arabidopsis,but little research on DMR genes has been reported in Brassica rapa and Brassica oleracea,the genomes of which exhibit significant differences in TE content.In this study,we identified 78 and 77 DMR genes in Brassica rapa and Brassica oleracea,respectively.Detailed analysis revealed that the numbers of DMR genes in different DMR pathways varied in B.rapa and B.oleracea.The evolutionary selection pressure of DMR genes in B.rapa and B.oleracea was compared,and the DMR genes showed differential evolution between these two species.The nucleotide diversity(π)and selective sweep(Tajima’s D)revealed footprints of selection in the B.rapa and B.oleracea populations.Transcriptome analysis showed that most DMR genes exhibited similar expression characteristics in B.rapa and B.oleracea.This study dissects the evolutionary differences and genetic variations of the DMR genes in B.rapa and B.oleracea,and will provide valuable resources for future research on the divergent evolution of DNA methylation between B.rapa and B.oleracea.

关键词： DNA methylation Brassica rapa Brassica oleracea evolutionary selection genetic variation gene expression

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含碳铁泥基催化剂活化H_(2)O_(2)处理亚甲基蓝废水

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工业水处理 2024年第2期44卷 125-132页

作者：王俩吴霜程小双梁澜李宁陈冠益侯立安天津大学环境科学与工程学院天津300350 中环保水务投资有限公司北京100082 天津商业大学机械工程学院天津300134

利用铁泥基催化剂构建类Fenton体系可实现污水厂废弃铁泥的资源化利用。热解温度可调控催化剂表面金属和非金属位点组成,影响其对过氧化氢(H_(2)O_(2))的活化效果。对不同热解温度(400~700℃)下制备的催化剂进行形貌、组成、结构和活性... 详细信息

利用铁泥基催化剂构建类Fenton体系可实现污水厂废弃铁泥的资源化利用。热解温度可调控催化剂表面金属和非金属位点组成,影响其对过氧化氢(H_(2)O_(2))的活化效果。对不同热解温度(400~700℃)下制备的催化剂进行形貌、组成、结构和活性等方面的测试。研究发现,700℃下制备的催化剂活化H_(2)O_(2),在100 min内对亚甲基蓝(MB)降解率达92.0%。当热解温度在400~700℃范围内增大时,催化剂比表面积和C含量均增大。机理探究表明,催化剂表面的Fe0、C==C/C—C和C==O活性位点对活化H_(2)O_(2)发挥重要作用,有利于促进亚甲基蓝降解。此外,铁泥基催化剂/H_(2)O_(2)体系中羟基自由基(·OH)和单线态氧(^(1)O_(2))是主要活性物种,超氧自由基(O_(2)^(·-))发挥作用较小。

关键词：废弃铁泥类Fenton 染料废水

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大气压固体分析探针结合单四极杆质谱仪快速检测18种合成大麻素

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质谱学报 2024年第2期45卷 292-300页

作者：许中石陈涛江柯成姚伟宣王继业吴元钊王斌杰李国军浙江警察学院浙江杭州310000 杭州市公安局交通警察支队西湖风景名胜区大队浙江杭州310000

本研究建立了一种大气压固体分析探针结合单四极杆质谱仪(AsaP-MS)快速检测18种合成大麻素(SCs)的方法。通过优化AsaP-MS锥孔电压,确定4种优化电压为15、25、35和50 V,以提供化合物全面的质谱信息。在优化条件下对一系列SCs标准溶液进... 详细信息

本研究建立了一种大气压固体分析探针结合单四极杆质谱仪(AsaP-MS)快速检测18种合成大麻素(SCs)的方法。通过优化AsaP-MS锥孔电压,确定4种优化电压为15、25、35和50 V,以提供化合物全面的质谱信息。在优化条件下对一系列SCs标准溶液进行分析,建立了18种SCs的质谱库,并对本方法进行方法学验证,得到18种SCs的检出限为10~20 mg/L。应用该方法对缴获的15批次未知样品进行检测,9批次呈阳性。该方法具有前处理简单、分析速度快、匹配高效精准、定性准确的优势,可用于基层公安禁毒部门的分析检测。

关键词：合成大麻素大气压固体分析探针结合单四极杆质谱(AsaP-MS) 快速检测自建库

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10μm 1280×1024 HgCdTe中波红外焦平面阵列探测性能提升

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红外与毫米波学报 2024年第1期43卷 36-43页

作者：谭必松毛剑宏陈殊璇李伟伟陈世锐陈天晴杜宇彭成盼熊雄周永强余波王舒浙江珏芯微电子有限公司浙江丽水323000

对像元尺寸为10μm的1280×1024碲镉汞(HgCdTe)中波红外焦平面阵列的制备技术和性能进行了研究。通过B+注入制备小尺寸n-on-p平面结;采用高平整度HgCdTe外延材料和高精度的倒焊互连技术,实现高的电学连通率;采用多段温度填胶固化和边缘... 详细信息

对像元尺寸为10μm的1280×1024碲镉汞(HgCdTe)中波红外焦平面阵列的制备技术和性能进行了研究。通过B+注入制备小尺寸n-on-p平面结;采用高平整度HgCdTe外延材料和高精度的倒焊互连技术,实现高的电学连通率;采用多段温度填胶固化和边缘刻蚀工艺减轻HgCdTe器件和读出集成电路(ROICs)之间的热失配,从而降低焦平面器件失效率。在85 K焦平面工作温度下,研制探测器的光谱响应范围为3.67μm至4.88μm,有效像元率高达99.95%,并且探测器组件像元的平均噪声等效温差(NETD)和暗电流密度的平均值分别小于16 mK和2.1×10^(-8)A/cm^(2)。与像元尺寸为15μm的探测器相比,10μm的1280×1024中波红外探测器可获取更加精细的图像,具有更远的识别距离。目前,该技术已成功转移到浙江珏芯微电子有限公司(ZJM)的HgCdTe红外探测器产线。

关键词：红外探测器碲镉汞 1 K×1 K红外焦平面阵列 n-on-p

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