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Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor

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Food Science and Human Wellness 2024年第2期13卷 823-829页

作者： Sai Wang rui ma Chengqiang Li Ling Zhang Haiyang Zhang Xuehan Li Xiangzhao mao Qingdao Key Laboratory of Food Biotechnology College of Food Science and EngineeringOcean University of ChinaQingdao 266404China Key Laboratory of Biological Processing of Aquatic Products China National Light IndustryQingdao 266404China Laboratory for marine Drugs and Bioproducts of Qingdao National Laboratory for marine Science and Technology Qingdao 266237China

Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.

关键词： Phosphatidylserine Chimeric aptamer AuNPs nanozyme Colorimetric aptasensor

Efficient second and third harmonic generation in dual-layer lithium niobate microdisk resonator

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Science China(Physics,Mechanics & Astronomy) 2024年第5期67卷 33-38页

作者： Zhenzhong Hao Xiao Wu Qiang Luo Zhang Li rui ma Fang Bo Feng Gao Guoquan Zhang Jingjun Xu MOE Key Laboratory of Weak-Light Nonlinear Photonics TEDA Institute of Applied Physics and School of PhysicsNankai UniversityTianjin 300457China

Lithium niobate thin film frequency doubler has extensive applications in the preparation of classical and quantum sources.In this study,we successfully fabricated microdisk resonators with a quality factor of 2.2×10^(5) in reverse-polarization dual-layer x-cut lithium niobate for the first time.Based on the modal phase matching condition,efficient second harmonic generation with a record normalized conversion efficiency of~56000%W-1 and cascaded third harmonic generation with an efficiency of~6500%W-2 were obtained in the microdisk resonator.Compared with the periodically poled lithium niobate microcavity,the complex domain structure preparation processes are avoided.Our work provides a scheme for achieving highly efficient second-order nonlinear effects in non-periodically poled microcavities.

关键词： harmonic lithium niobate

digital micromirror device;flat-top beam;orbital angular momentum;perfect vortex beam;random fiber laser

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High Power Laser Science and Engineering 2024年第1期12卷 42-51页

作者： rui ma Ke Hai Luo Jing Song He Wei Li Zhang Dian Yuan Fan Anderson S.L.Gomes Jun Liu International Collaborative Laboratory of 2D materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhenChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Fiber Optics Research Centre School of Information and Communication EngineeringUniversity of Electronic Science and Technology of ChinaChengduChina Departamento de Física Universidade Federal de PernambucoRecife-PEBrazil

High-intensity vortex beams with tunable topological charges and low coherence are highly demanded in applications such as inertial confinement fusion(ICF) and optical communication. However, traditional optical vortices featuring nonuniform intensity distributions are dramatically restricted in application scenarios that require a high-intensity vortex beam owing to their ineffective amplification resulting from the intensity-dependent nonlinear effect. Here, a low-coherence perfect vortex beam(PVB) with a topological charge as high as 140 is realized based on the super-pixel wavefront-shaping technique. More importantly, a globally adaptive feedback algorithm(GAFA) is proposed to efficiently suppress the original intensity fluctuation and achieve a flat-top PVB with dramatically reduced beam speckle contrast. The GAFA-based flat-top PVB generation method can pave the way for high-intensity vortex beam generation,which is crucial for potential applications in ICF, laser processing, optical communication and optical trapping.

关键词： digital micromirror device flat-top beam orbital angular momentum perfect vortex beam random fiber laser

Information‑entropy enabled identifying topological photonic phase in real space

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Frontiers of Optoelectronics 2024年第2期17卷 1-9页

作者： rui ma Qiuchen Yan Yihao Luo Yandong Li Xingyuan Wang Cuicui Lu Xiaoyong Hu Qihuang Gong State Key Laboratory for Mesoscopic Physics&Department of Physics Collaborative Innovation Center of Quantum Matter&Frontiers Science Center for Nano‑OptoelectronicsPeking UniversityBeijing 100871China The MOE Key Laboratory of Weak‑Light Nonlinear Photonics TEDA Applied Physics Institute and School of PhysicsNankai UniversityTianjin 300457China College of mathematics and Physics Beijing University of Chemical TechnologyBeijing 100029China Laboratory of Advanced Optoelectronic Quantum Architecture and Measurements of Ministry of Education Beijing Key Laboratory of Nanophotonics and Ultrafine Optoelectronic SystemsSchool of PhysicsBeijing Institute of TechnologyBeijing 100081China Peking University Yangtze Delta Institute of Optoelectronics Nantong 226010China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Hefei National Laboratory Hefei 230088China Beijing Academy of Quantum Information Sciences Beijing 100193China

The topological photonics plays an important role in the fields of fundamental physics and photonic devices.The traditional method of designing topological system is based on the momentum space,which is not a direct and convenient way to grasp the topological properties,especially for the perturbative structures or coupled systems.Here,we propose an interdisciplinary approach to study the topological systems in real space through combining the information entropy and topological photonics.As a proof of concept,the Kagome model has been analyzed with information entropy.We reveal that the bandgap closing does not correspond to the topological edge state disappearing.This method can be used to identify the topological phase conveniently and directly,even the systems with perturbations or couplings.As a promotional validation,Su-Schrieffer-Heeger model and the valley-Hall photonic crystal have also been studied based on the information entropy method.This work provides a method to study topological photonic phase based on information theory,and brings inspiration to analyze the physical properties by taking advantage of interdisciplinarity.

关键词： Information entropy Kagome model Topological photonic phase

Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy

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Light(Science & Applications) 2024年第5期13卷 894-907页

作者： Fei Yang Zhiyang Wang Wenbin Shi Miao Wang rui ma Wuyu Zhang Xipeng Li Erqi Wang Wenjie Xie Zhan Zhang Qi Shen Feifan Zhou Sihua Yang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China. Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China. Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical EngineeringHainan UniversityHaikou 570100China. Department of Neurology Sun Yatsen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China. Guangdong Province Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510120China. Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of matter South China Normal UniversityGuangzhou 510006China

Meningeal lymphatic vessels(mLVs)play a pivotal role in regulating metabolic waste from cerebrospinal fluid(CSF).However,the current limitations in field of view and resolution of existing imaging techniques impede understanding the stereoscopic morphology and dynamic behavior of mLVs in vivo.Here,we utilized dual-contrast functional photoacoustic microscopy to achieve wide-field intravital imaging of the lymphatic system,including mLVs and glymphatic pathways.The stereoscopic photoacoustic microscopy based on opto-acoustic confocal features has a depth imaging capability of 3.75 mm,facilitating differentiation between mLVs on the meninges and glymphatic pathways within the brain parenchyma.Subsequently,using this imaging technique,we were able to visualize the dynamic drainage of mLVs and identify a peak drainage period occurring around 20–40 min after injection,along with determining the flow direction from CSF to lymph nodes.Inspiringly,in the Alzheimer’s disease(AD)mouse model,we observed that AD mice exhibit a~70%reduction in drainage volume of mLVs compared to wild-type mice.With the development of AD,there is be continued decline in mLVs drainage volume.This finding clearly demonstrates that the AD mouse model has impaired CSF drainage.Our study opens up a horizon for understanding the brain’s drainage mechanism and dissecting mLVs-associated neurological disorders.

关键词： lymphatic impaired field

Contemporary Asymmetric Genetic Introgression Between Two Pelophylax Species in Shanghai

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Asian Herpetological Research 2024年第1期15卷 1-11页

作者： Xu WEI Wenli LIU Gang WANG rui ma Meiling HUANG Jiaxin ZHENG Tingwei DONG Changqing YUAN Shunqi BO Xiao YUAN Ben LI Shuo ma Qu YUE Youzhong DING Zhenghuan WANG School of Life Sciences East China Normal UniversityShanghai 200241China Shanghai Landscaping and City Appearance Administrative Bureau Shanghai 200041China State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200241China Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration East China Normal UniversityShanghai 200241China Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station Ministry of Education&Shanghai Science and Technology CommitteeShanghai 202162China

Human disturbances are considered to break reproduction barriers among species.Significant increases in hybridization events have been reported among a large number of taxonomic groups in anthropogenic environments,providing novel insights into species evolution mechanisms and conservation management in the Anthropocene.The Eastern Golden Frog(Pelophylax plancyi)and BlackSpotted Frog(P.nigromaculatus)are two sympatric anuran species with a long history of mitochondrial genome introgression in highly urbanized continental East Asia.However,there is only limited understanding of the pattern of their contemporary hybridization and factors influencing their interspecific relationship under anthropogenic disturbances.Here,interspecific hybridization between P.plancyi and P.nigromaculatus at the population level was investigated in Shanghai.All except two haplotypes obtained from both species in Shanghai were mixed together,and located in the introgression clade,implying multiple ancient mitochondrial introgression events occurred in the populations of our study area.Asymmetric genetic introgression was detected by microsatellite markers,with 0.7%of P.plancyi and 14.6%of P.nigromaculatus identified as contemporary admixed individuals.Consistent with the trend of population density,higher genetic diversity of neutral microsatellite loci was found in the more abundant P.plancyi;however,variation in mitochondrial(Cyt-b)and nuclear(POMC)genes was higher in relatively rare P.nigromaculatus.The population density of P.plancyi and number of water patches within local habitats were significantly positively correlated with both occurrences and proportions of admixed individuals in the populations of P.plancyi and P.nigromaculatus.Considering the prevalent transformation of habitats in urbanized areas,these results imply that a high population density in isolated artificially altered habitats is likely to increase interspecific hybridization.Thus,population monitoring and improvement of landscape connectivity between habitats wo

关键词： anthropogenic disturbances interspecific hybridization Pelophylax species abundance urban landscape urbanization

Confined Mn^(2+) enables effective aerobic oxidation catalysis

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Science China Chemistry 2024年第5期67卷 1545-1553页

作者： Desheng Yuan Sicong ma Xiao Kong Chi Zhang Lin Chen Chengsheng Yang Lihua Wang Zhen Liu Lin Ye Yongmei Liu rui ma Zhi-Pan Liu Yifeng Zhu Yong Cao Xinhe Bao Department of Chemical Physics University of Science and Technology of ChinaHefei 230026China Department of Chemistry Shanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsCollaborative Innovation Center of Chemistry for Energy MaterialsFudan UniversityShanghai 200438China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China School of materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China Institute of Advanced Fluorine-Containing materials Zhejiang Normal UniversityJinhua 321004China State Key Laboratory of Catalysis National Laboratory for Clean EnergyCollaborative Innovation Center of Chemistry for Energy MaterialsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China

Effective and mild activation of O_(2) is essential but challenging for aerobic oxidation. In heterogeneous catalysis, high-valence manganese oxide(e.g., +4) is known to be active for the oxidation, whereas divalent MnO is ineffective due to its limited capacity to supply surface oxygen and its thermodynamically unstable structure when binding O_(2) in reaction conditions. Inspired by natural enzymes that rely on divalent Mn^(2+), we discovered that confining Mn^(2+) onto the Mn_(2)O_(3) surface through a dedicated calcination process creates highly active catalysts for the aerobic oxidation of 5-hydroxymethylfurfural, benzyl alcohol, and CO.The Mn_(2)O_(3)-confined Mn^(2+) is undercoordinated and efficiently mediates O_(2) activation, resulting in 2–3 orders of magnitude higher activity than Mn_(2)O_(3) alone. Through low-temperature infrared spectroscopy, we distinguished low-content Mn^(2+) sites at Mn_(2)O_(3) surface, which are difficult to be differentiated by X-ray photoelectron spectroscopy. The combination of in-situ energydispersive X-ray absorption spectroscopy and X-ray diffraction further provides insights into the formation of the newly identified active Mn^(2+) sites. By optimizing the calcination step, we were able to increase the catalytic activity threefold further.The finding offers promising frontiers for exploring active oxidation catalysts by utilizing the confinement of Mn^(2+)and oftenignored calcination skills.

关键词： confinement catalysis manganese oxide aerobic oxidation divalent Mn^(2+) operando spectroscopies

Countermeasure against blinding attack for single-photon detectors in quantum key distribution

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Journal of Semiconductors 2024年第4期45卷 76-81页

作者： Lianjun Jiang Dongdong Li Yuqiang Fang Meisheng Zhao Ming Liu Zhilin Xie Yukang Zhao Yanlin Tang Wei Jiang Houlin Fang rui ma Lei Cheng Weifeng Yang Songtao Han Shibiao Tang QuantumCTek Co. Ltd.Hefei 230088China Shandong Institute of Quantum Science and Technology Co. Ltd.Jinan 250101China QuantumCTek(Beijing)Co. Ltd.Beijing 100193China School of Cyber Science and Technology University of Science and Technology of ChinaHefei 230027China Anhui Armed Police Force Hefei 230026China School of Electrical Engineering and Automation Anhui UniversityHefei 230601China

Quantum key distribution(QKD),rooted in quantum mechanics,offers information-theoretic security.However,practi-cal systems open security threats due to imperfections,notably bright-light blinding attacks targeting single-photon detectors.Here,we propose a concise,robust defense strategy for protecting single-photon detectors in QKD systems against blinding attacks.Our strategy uses a dual approach:detecting the bias current of the avalanche photodiode(APD)to defend against con-tinuous-wave blinding attacks,and monitoring the avalanche amplitude to protect against pulsed blinding attacks.By integrat-ing these two branches,the proposed solution effectively identifies and mitigates a wide range of bright light injection attempts,significantly enhancing the resilience of QKD systems against various bright-light blinding attacks.This method forti-fies the safeguards of quantum communications and offers a crucial contribution to the field of quantum information security.

关键词： quantum key distribution single photon detector blinding attack pulsed blinding attack countermeasure quan-tum communication

Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review

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Infectious Diseases of Poverty 2024年第1期13卷 92-92页

作者： rui ma Chunfu Li Ai Gao Na Jiang Xinyu Feng Jian Li Wei Hu School of Life Sciences Inner Mongolia University Hohhot China School of Global Health Chinese Center for Tropical Diseases Research Shanghai Jiao Tong University School of Medicine Shanghai China One Health Center Shanghai Jiao Tong University-The University of Edinburgh Shanghai China Basic Medical College Guangxi University of Chinese Medical Nanning China Department of Infectious Diseases Huashan Hospital State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Ministry of Education Key Laboratory of Contemporary Anthropology School of Life Sciences Fudan University Shanghai China

Background Brucellosis is a zoonotic affiction instigated by bacteria belonging to the genus Brucella and is charac-terized by a diverse range of pervasiveness,multiple transmission routes,and serious hazards.It is imperative to amal-gamate the current knowledge and identify gaps pertaining to the role of ticks in brucellosis transmission.Methods We systematically searched China National Knowledge Infrastructure(CNKi),WanFang,Google Scholar,and PubMed on the topic published until April 23,2022.The procedure was performed in accordance with the Sys-tematic Reviews and Meta-Analyses extension for Scoping Reviews(PRISma-ScR)guidelines.The selected articles were categorized across three major topic areas,and the potential data was extracted to describe evidence-practice gaps by two reviewers.Results The search identifed 83 eligible studies for the final analyses.The results highlighted the potential capacity of ticks in brucellosis transmission as evidenced by the detection of Brucella in 16 different tick species.The pooled overall prevalence of Brucella in ticks was 33.87%(range:0.00-87.80%).The review also revealed the capability of Bru-cella to circulate in parasitic ticks'different developmental stages,thus posing a potential threat to animal and human health.Empirical evidence from in vitro rodent infection experiments has revealed that ticks possess the capability to transmit Brucella to uninfected animals(range:45.00-80.00%).Moreover,significant epidemiological associations have been found between the occurrence of brucellosis in animals and tick control in rangelands,which further suggests that ticks may serve as potential vectors for brucellosis transmission in ruminants.Notably,a mere three cases of human brucellosis resulting from potential tick bites were identified in search of global clinical case reports from 1963to 2019.Conclusions It is imperative to improve the techniques used to identify Brucella in ticks,particularly by developing a novel,efficient,precise approach that can be applied in a field

关键词： Tick Brucella Detection Human infection Risk evaluation