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Deciphering mycorrhizal fungi in cultivated Phalaenopsis microbiome with next-generation sequencing of multiple barcodes

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Fungal Diversity 2014年第3期66卷 77-88页

作者： Chao-Li huang Feng-Yin Jian Hao-Jen huang Wen-Chi Chang Wen-Luan Wu Chi-Chuan Hwang ruey-hua lee Tzen-Yuh Chiang Department of Life Sciences National Cheng Kung UniversityTainanTaiwan 701 Institute of Tropical Plant Sciences National Cheng Kung UniversityTainanTaiwan 701 Department of Engineering Science and Supercomputing Research Center National Cheng Kung UniversityTainanTaiwan 701

Identifying the species composition of a microbial ecosystem is often hampered by difficulties in culturing the organisms and in the low sequencing depth of traditional DNA *** analysis,a huge-scale nucleotide-sequence-based tool,can overcome such *** this study,Sanger sequencing of 500 nrITS clones uncovered 29 taxa of 19 fungal genera,whereas metagenomics with next-generation sequencing identified 512 operational taxonomic units(OTUs)for ITS1/2 and 364 for ITS3/***,high throughput sequencing of PCR amplicons of ITS1/2,ITS3/4,nrLSU-LR,nrLSU-U,mtLSU,and mtATP6,all with at least 1,300×coverage and about 21 million reads in total,yielded a very diverse fungal *** fact that 74%of the OTUs were exclusively uncovered with single barcodes indicated that each marker provided its own insights into the fungal *** deal with the high heterogeneity in the data and to integrate the information on species composition across barcodes,a rank-scoring strategy was ***,205 genera among 64 orders of fungi were identified in healthy Phalaenopsis *** the barcodes utilized,ITS1/2,ITS3/4,and nrLSU-U were the most competent in uncovering the fungal *** barcodes,though detecting different compositions likely due to primer preference,provided complementary and comprehensive power in deciphering the microbial diversity,especially in revealing rare species.

关键词： DNA barcode Metagenomics Mycorrhizal fungi Next-generation sequencing Phalaenopsis orchid Rank scoring

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Surface-phonon-polariton-enhanced photoinduced dipole force for nanoscale infrared imaging

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National Science Review 2024年第5期11卷 227-235页

作者： Jian Li Junghoon Jahng Xuezhi Ma Jing Liang Xue Zhang Qianhao Min Xiao-Liang Wang Shuangjun Chen Eun Seong lee Xing-hua Xia State Key Lab of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Hyperspectral Nano-Imaging Team Korea Research Institute of Standards and Science Institute of Materials Research and Engineering Agency for ScienceTechnology and Research College of Materials Science and Engineering Nanjing Tech University

The photoinduced dipole force(Pi DF) is an attractive force arising from the Coulombic interaction between the light-induced dipoles on the illuminated tip and the sample. It shows extreme sample-tip distance and refractive index dependence, which is promising for nanoscale infrared(IR) imaging of ultrathin samples. However, the existence of Pi DF in the mid-IR region has not been experimentally demonstrated due to the coexistence of photoinduced thermal force(Pi TF), typically one to two orders of magnitude higher than Pi DF. In this study, we demonstrate that, with the assistance of surface phonon polaritons, the Pi DF of c-quartz can be enhanced to surpass its Pi TF, enabling a clear observation of Pi DF spectra reflecting the properties of the real part of permittivity. Leveraging the detection of the Pi DF of phonon polaritonic substrate, we propose a strategy to enhance the sensitivity and contrast of photoinduced force responses in transmission images, facilitating the precise differentiation of the heterogeneous distribution of ultrathin samples.

关键词： surface phonon polariton photoinduced dipole force nanoscale infrared imaging ultrathin sample

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Improving lung allograft function in the early post-operative period through the inhibition of pyroptosis

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Medical Review 2024年第5期4卷 384-394页

作者： Doug A.Gouchoe Zhentao Zhang Jung-Lye Kim Yong Gyu lee Bryan A.Whitson hua Zhu Department of Surgery Division of Cardiac SurgeryThe Ohio State University Wexner Medical CenterColumbusOHUSA COPPER Laboratory The Ohio State University Wexner Medical CenterColumbusOHUSA Cardiac Surgery The Ohio State University Wexner Medical CenterColumbusOH 43210USA

Lung transplantation is the only deﬁnitive ther-apy for end-stage pulmonary *** than 20%of offered lungs are successfully transplanted due to a limited ischemic time window and poor donor lung quality man-ifested by pulmonary edema,hypoxia,or ***,poor donor organ recovery and utilization are signiﬁcant barriers to wider implementation of the life-saving therapy of *** ischemia reperfusion injury(IRI)is often identiﬁed as the underlying molecular insult leading to immediate poor lung function in the post-operative period,this injury encompasses several pathways of cellular injury in addition to the recruitment of the innate immune system to the site of injury to propagate this inflammatory *** is a central molecular inflammatory pathway that is the most signiﬁcant contributor to injury in this early post-operative *** is another form of pro-grammed cell death and is often associated with *** mitigation of pyroptosis in the early post-operative period following lung transplantation is a potential novel way to prevent poor allograft function and improve outcomes for all *** we detail the pyroptotic pathway,its importance in lung transplantation,and several therapeutic modalities that can mitigate this harmful inflammatory pathway.

关键词： lung transplantation pyroptosis ischemia reperfusion injury IRI ex vivo lung perfusion EVLP

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Comparative associations of non-alcoholic fatty liver disease and metabolic dysfunction-associated steatotic liver disease with risk of incident chronic kidney disease:a cohort study

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Hepatobiliary Surgery and Nutrition 2024年第5期13卷 801-813,I0003-I0005页

作者： Ji Hye Heo Mi Yeon lee Seong Hwan Kim Ming-hua Zheng Christopher D.Byrne Giovanni Targher Ki-Chul Sung Department of Internal Medicine Hallym University College of MedicineChuncheonRepublic of Korea Division of Biostatistics Department of R&D ManagementKangbuk Samsung HospitalSungkyunkwan University School of MedicineSeoulRepublic of Korea Division of Cardiology Department of Internal MedicineKorea University Ansan HospitalAnsanRepublic of Korea MAFLD Research Center Department of HepatologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Wenzhou Key Laboratory of Hepatology WenzhouChina Institute of Hepatology Wenzhou Medical UniversityWenzhouChina Key Laboratory of Diagnosis and Treatment for the Development of Chronic Liver Disease in Zhejiang Province WenzhouChina Nutrition and Metabolism Faculty of MedicineUniversity of SouthamptonSouthamptonUK Southampton National Institute for Health and Care Research Biomedical Research CentreUniversity Hospital SouthamptonSouthamptonUK Metabolic Diseases Research Unit IRCCS Sacro Cuore-Don Calabria HospitalNegrar di ValpolicelleItaly Division of Cardiology Department of Internal MedicineKangbuk Samsung HospitalSungkyunkwan University School of MedicineSeoulRepublic of Korea

Background:We examined the comparative associations between non-alcoholic fatty liver disease(NAFLD)and metabolic dysfunction-associated steatotic liver disease(MASLD)definitions with risk of developing chronic kidney disease(CKD)and abnormal ***:We conducted a cohort study of 214,145 Korean adults with normal kidney function at baseline who underwent liver *** were further subdivided into no steatotic liver disease(no-SLD),NAFLD-only,MASLD-only,both NAFLD and MASLD,and SLD not categorized as NAFLD or MASLD *** proportional hazards models were used to analyze the risk of incident CKD and ***:Compared with either the no-NAFLD or no-MASLD groups,the NAFLD and MASLD groups were associated with a higher risk of incident CKD(NAFLD:adjusted hazard ratio(HR),1.18[95%CI,1.01-1.38];MASLD:adjusted HR,1.21[95%CI,1.04-1.39]).Among the five subgroups,both NAFLD and MASLD group had the strongest association with risk of incident CKD(adjusted HR,1.21[95%CI,1.04-1.42]).The MASLD-only group had the strongest association with incident abnormal albuminuria,with an adjusted HR comparable to that of the both NAFLD and MASLD group(adjusted HR 1.96[95%CI,1.44-2.67]for the MASLD-only,and adjusted HR 1.98[95%CI,1.58-2.49]for the both NAFLD and MASLD group versus the no-SLD group).The NAFLD-only group was not independently associated with risk of CKD or abnormal ***:These findings suggest that MASLD definition identifies individuals at high risk of developing incident CKD or abnormal albuminuria better than NAFLD definition.

关键词： Metabolic dysregulation non-alcoholic fatty liver disease(NAFLD) metabolic syndrome chronic kidney disease(CKD) albuminuria

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Breeding Asian seabass to increase survival against big belly disease and growth

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Aquaculture and Fisheries 2024年第5期9卷 731-738页

作者： Peng Liu Jun Hong Xia Fei Sun Le Wang Zituo Yang May lee Hong Yan Pang Yan Fei Wen Gen hua Yue Temasek Life Sciences Laboratory National University of Singapore1 Research Link117604Singapore Department of Biological Sciences National University of Singapore14 Science Drive117543Singapore

Asian seabass(Lates calcarifer)is an important foodfish *** has been cultured in Singapore since the 1980s.A selective breeding program to improve growth,meat quality and disease resistance has been ongoing in Singapore since *** 2012,outbreaks of the big belly disease(BBD)occurred in two batches of offspring generated by a mass-cross among F1 brooders in a *** the outbreaks,only 437 survived in 6000 F2 *** parentage analysis with 10 microsatellites revealed that four parents(F1)produced high percentage of offspring that survived the BBD ***-two BBD survivor offspring(F2)were selected based on their growth performance at 90 days post hatch(dph),family origin and allelic *** were used as brooders to generate *** a period of five years,these F2 brooders produced six batches of offspring,where no outbreak of BBD *** the same period,outbreaks of BBD occurred in 4 out of 18 batches produced by F2 brooders selected for growth,with mortality rates ranging from 85.1%to 95.4%.In F3,the average body weight(57.1±1.09 g,n=3068)of the line for BBD survival was slightly lower than that(60.6±2.29 g,n=4280)of the line for growth at 90 *** both lines,the body weight of F3 was much higher than that(46.90±0.19 g,n=12117)of *** genetic diversity in the line for BBD survival(F3)was much lower than that in F3 line for growth due to small founder *** data indicate that the selection of BBD survival during BBD outbreaks using molecular parentage analysis is effective to reduce BBD *** further reduction of genetic diversity is essential in the line selected for increased survival against BBD.

关键词： Barramundi Infection Resistance Growth Marker Diversity

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Characteristics of Biopellets Manufactured from Various Lignocellulosic Feedstocks as Alternative Renewable Energy Sources

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Journal of Renewable Materials 2024年第6期12卷 1103-1123页

作者： Anggara Ridho Putra Apri Heri Iswanto Arif Nuryawan Saptadi Darmawan ElvaraWindra Madyaratri Widya Fatriasari lee Seng hua Petar Antov Harisyah Manurung Ade Pera Amydha Sudrajat Herawati Pendi Department of Forest Products Faculty of ForestryUniversitas Sumatera Utara.Kampus USU Padang BulanMedan20155Indonesia Research Center for Biomass and Bioproducts National Research and Innovation Agency(BRIN)Jl Raya Bogor KM 46 CibinongBogor16911Indonesia Department of Wood Industry Faculty of Applied SciencesUniversiti Teknologi MARA(UiTM)Cawangan Pahang Kampus JengkaShah Alam26400Malaysia Faculty of Forest Industry University of ForestrySofia1797Bulgaria

The increased valorization of renewable and cost-effective lignocellulosic feedstocks represents a viable,sustainable,and eco-friendly approach toward the production of biopellets as alternative energy *** aim of this research work was to investigate and evaluate the feasibility of using various lignocellulosic raw materials,i.e.,raru(Cotylelobium melanoxylon),mangrove(Rhizophora spp.),sengon(Paraserianthes falcataria),kemenyan toba(Styrax sumatrana),oil palm(Elaeis guineensis),manau rattan(Calamus manan),and belangke bamboo(Gigantochloa pruriens)for manufacturing biopellets with different particle *** raw materials used were tested for their moisture content,specific gravity,ash,cellulose,and lignin *** addition,thermal analyses,i.e.,calorific values,thermogravimetric analysis(TGA),and differential scanning calorimetry(DSC),were *** following properties of the biopellets produced were investigated:moisture content,volatile matter,ash content,fixed carbon,density,and thermal *** on an analysis of the raw materials,raru had the lowest moisture content(12%)and ash content(1.5%)and the highest specific gravity(1.2).Markedly,palm oil stem had the highestα-cellulose(55%)and lignin(37%)*** accordance with the SNI 8675:2018 standard requirements,biopellets with optimal properties(moisture content of 1.4%,ash content of 0.79%,density of 1.09 g/m^(3),calorific value of 4672 cal/g,and TGA residue of 13.9%),were manufactured from raru wood.

关键词： Biomass biopellet renewable energy particle size

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Color liquid crystal grating based color holographic 3D display system with large viewing angle

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Light(Science & Applications) 2024年第1期13卷 103-112页

作者： Di Wang Yi-Long Li Fan Chu Nan-Nan Li Zhao-Song Li Sin-Doo lee Zhong-Quan Nie Chao Liu Qiong-hua Wang School of Instrumentation and Optoelectronic Engineering Beihang UniversityBeijing 100191China State Key Laboratory of Virtual Reality Technology and Systems Beihang UniversityBeijing 100191China Display Technology Research Center Seoul National UniversityGwanak-ro 1Gwanak-guSeoul 08826Republic of Korea Key Lab of Advanced Transducers and Intelligent Control System Ministry of EducationTaiyuan University of TechnologyTaiyuan 030024China

Holographic 3D display is highly desirable for numerous applications ranging from medical treatments to military ***,it is challenging to simultaneously achieve large viewing angle and high-fidelity color reconstruction due to the intractable constraints of existing ***,we conceptually propose and experimentally demonstrate a simple and feasible pathway of using a well-designed color liquid crystal grating to overcome the inevitable chromatic aberration and enlarge the holographic viewing angle,thus enabling large-viewing-angle and color holographic 3D *** use of color liquid crystal grating allows performing secondary diffraction modulation on red,green and blue reproduced images simultaneously and extending the viewing angle in the holographic 3D display *** principle,a chromatic aberration-free hologram generation mechanism in combination with the color liquid crystal grating is proposed to pave the way for on such a superior holographic 3D *** proposed system shows a color viewing angle of~50.12°,which is about 7 times that of the traditional system with a single spatial light *** work presents a paradigm for achieving desirable holographic 3D display,and is expected to provide a new way for the wide application of holographic display.

关键词： holographic angle crystal

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Thermo-Mechanical, Physico-Chemical, Morphological, and Fire Characteristics of Eco-Friendly Particleboard Manufactured with Phosphorylated Lignin Addition

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Journal of Renewable Materials 2024年第7期12卷 1311-1341页

作者： Apri Heri Iswanto Harisyah Manurung Asma Sohail lee Seng hua Petar Antov Deded Sarip Nawawi Sarah Latifah Dewi Shafa Kayla Sukma Surya Kusumah Muhammad Adly Rahandi Lubis Linda MakovickáOsvaldová MohdHazwan Hussin Rangabhashiyam Selvasembian Lum Wei Chen Puji Rahmawati Nurcahyani Nam Hun Kim Widya Fatriasari Department of Forest Products Faculty of ForestryUniversitas Sumatera UtaraKampus USU Padang BulanMedanNorth Sumatra20155Indonesia Department of Chemistry Lahore College for Women UniversityLahore4444Pakistan Department of Wood Industry Faculty of Applied SciencesUniversiti Teknologi MARA(UiTM)Cawangan Pahang Kampus JengkaShah Alam26400Malaysia Faculty of Forest Industry University of ForestrySofia1797Bulgaria Department of Forest Product Faculty of Forestry and EnvironmentIPB UniversityBogor16680Indonesia Research Center for Biomass and Bioproducts National Research and Innovation Agency(BRIN)Cibinong16911Indonesia Department of Forest Engineering Faculty of Security EngineeringUniversity of ZilinaZilina01001Slovakia Materials Technology Research Group(MaTReC) School of Chemical SciencesUniversiti SainsMalaysiaMindenPenang11800Malaysia Department of Environmental Science and Engineering School of Engineering and SciencesSRM University-APAmaravatiAndhra Pradesh522240India Department of Bio and Natural Resources Faculty of Bioengineering and TechnologyUniversitiMalaysia KelantanJeliKelantan17600Malaysia Department of Forest Biomaterials Engineering College of Forest and Environmental SciencesKangwon National UniversityChuncheon24341Republic of Korea

Lignin,lignosulfonate,and synthesized phosphorylated lignosulfonate were introduced as greenﬁllers in citric acid-sucrose adhesives for bonding particleboard fabricated from areca leaf sheath(ALS).The characteristics of particleboards were compared to that of ultralow emitting formaldehyde(ULEF-UF).Theﬁllers derived from Eucalyptus ***-lignin were added forﬂame retardancy enhancement.10%of each lignin and modiﬁed lig-nin was added into the ULEF-UF and citric acid-sucrose bonded *** applied to particle-boards included thermal characteristics,X-ray diffraction analysis(XRD),morphological properties,Fourier transform infrared spectroscopy(FTIR),as well as physical,mechanical,andﬁre resistance characteristics of the laboratory-fabricated *** and modiﬁed lignin resulted in improved thermal stability of the composites bonded with ULEF-UF while the improvement in the particleboard bonded with citric acid-sucrose was not signiﬁ*** introduction ofﬁller exerted a higher inﬂuence on the UF-bonded particleboards compared to composites fabricated with citric ***,the presence of lignin,lignosulfonate,and phosphorylated lignosulfonate enhanced the mechanical strength of the ULEF-bonded particleboards,although their dimensional stability has ***,the use of lignin and lignosulfonate enhanced theﬁre resis-tance of the particleboards produced with lower observed weight *** laboratory particleboards exhibited satisfactoryﬁre resistance,attaining a V-0 rating in according to the UL-94 standard.

关键词： Modiﬁed lignin eco-friendly particleboard ﬁre and thermal properties citric acid-sucrose ultralow-emitting urea-formaldehyde resin

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Scintillation Arc from FRB 20220912A

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 134-142页

作者： Zi-Wei Wu Robert AMain Wei-Wei Zhu Bing Zhang Peng Jiang Jia-Rui Niu Jin-Lin Han Di Li Ke-Jia lee Dong-Zi Li Yuan-Pei Yang Fa-Yin Wang Rui Luo Pei Wang Chen-Hui Niu Heng Xu Bo-Jun Wang Wei-Yang Wang Yong-Kun Zhang Yi Feng De-Jiang Zhou Yong-hua Xu Can-Min Deng Yu-Hao Zhu National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Max-Planck-Institut für Radioastronomie Bonn 53121Germany Institute for Frontier in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China Nevada Center for Astrophysics and Department of Physics and Astronomy University of NevadaLas VegasNV 89154USA CAS Key Laboratory of FAST National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China School of Astronomy University of Chinese Academy of SciencesBeijing 100049China NAOC-UKZN Computational Astrophysics Centre University of KwaZulu-NatalDurban 4000South Africa Department of Astronomy Peking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Cahill Center for Astronomy and Astrophysics California Institute of TechnologyPasadena 91125USA South-Western Institute For Astronomy Research Yunnan UniversityKunming 650504China School of Astronomy and Space Science Nanjing UniversityNanjing 210093China Key Laboratory of Modern Astronomy and Astrophysics(Nanjing University) Ministry of EducationNanjing 210023China CSIRO Space and Astronomy Epping 1710Australia Department of Astronomy School of Physics and Materials ScienceGuangzhou UniversityGuangzhou 510006China Research Center for Intelligent Computing Platforms Zhejiang LaboratoryHangzhou 311100China Yunnan Observatories Chinese Academy of SciencesKunming 650011China Guangxi Key Laboratory for Relativistic Astrophysics Department of PhysicsGuangxi UniversityNanning 530004China

We present the interstellar scintillation analysis of fast radio burst(FRB)20220912A during its extremely active episode in 2022using data from the Five-hundred-meter Aperture Spherical Radio Telescope(FAST).We detect a scintillation arc in the FRB’s secondary spectrum,which describes the power in terms of the scattered FRB signals’time delay and Doppler *** arc indicates that the scintillation is caused by a highly localized *** analysis favors a Milky Way origin of the ionized interstellar medium(IISM)for the localized scattering medium but cannot rule out a host galaxy *** present our method for detecting the scintillation arc,which can be applied generally to sources with irregularly spaced bursts or *** methods could help shed light on the complex interstellar environment surrounding the FRBs and in our Galaxy.

关键词： fast radio burst scintillation FRB 20220912A

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超导能隙振荡: 到底来自配对密度波还是拆对散射?

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物理学报 2024年第15期73卷 5-7页

作者：殷嘉鑫王强华南方科技大学物理系深圳518055 南京大学物理学院固体微结构物理国家重点实验室南京210093

早在1965年,于渌先生发表在《物理学报》的经典理论工作就指出:超导能隙在磁性杂质周围会产生微弱的周期性振荡.近十年来,一系列高分辨率的扫描隧道显微实验观测到超导体内能隙在空间中的微弱振荡行为并指认为配对密度波.据此情况,李东... 详细信息

早在1965年,于渌先生发表在《物理学报》的经典理论工作就指出:超导能隙在磁性杂质周围会产生微弱的周期性振荡.近十年来,一系列高分辨率的扫描隧道显微实验观测到超导体内能隙在空间中的微弱振荡行为并指认为配对密度波.据此情况,李东海等理论学者近期指出,在多种情况下,杂质拆对散射构成的干涉效应也会导致能隙在空间形成周期性振荡.我们讨论这两种观点的对立与统一,以及它们和实验的联系.

关键词：超导配对密度波扫描隧道显微镜

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