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Facile and diverse logic circuits based on dumbbell DNA-templated fluorescent copper nanoclusters and S1 nuclease detection

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Chinese Chemical Letters 2022年第5期33卷 2711-2714页

作者： Zefeng Gu Anchen Fu ru qiu ru Sun Zhijuan Cao Shanghai Key Laboratory of Bioactive Small Molecules&Department of Pharmaceutical Analysis School of PharmacyFudan UniversityShanghai 201203China

DNA-based logic gates promote the development of molecular computing and show enormous potential in the fields of nanotechnology and biotechnology. Dumbbell oligonucleotides(DNA) with poly-thymine(poly-T) loops and a nicked random double strand have been demonstrated to be an efficient template for the formation of fluorescent copper nanoclusters(Cu NCs) in our previous work. Herein, a new platform technology is presented with which to construct molecular logic gates by employing Cu NCs probe as a basic output generator, coupling of functional nucleases as the inputs. Two dumbbell DNAs are used with the difference in stem length(8 bp and 16 bp, respectively). The degradation of DNA templates can be tuned by various nucleic acid enzymes, single-stranded nuclease(S1), double-stranded specific nuclease(DSN), E. coli DNA ligase, exonucleases Ⅰ and Ⅲ. Briefly, S1 can digest both DNA templates, while the cleavage ability of DSN will be resistant by the short stem of SS-DNA(short-stem DNA). Exonuclease Ⅰ and Ⅲ can degrade these two nicked DNA templates, which are inhibited due to the ligation of E. coli DNA ligase. With this novel strategy, a set of logic gates is successfully constructed at the molecular level,including “YES”, “PASS 0”, “OR”, “INHIBIT”, which take the advantages of no label, easy operation, fast speed, high efficiency and low cost. Furthermore, S1 nuclease, as the biomarker of numerous carcinogens,is selectively detected in the range of 0.05–50 U/m L with the detection limit of 0.005 U/m L(1×10^(−6)U)based on this platform.

关键词： Logic gates Dumbbell DNA templates Poly-thymine loops Copper nanoclusters Nucleic acid enzymes

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Synthesis, Structures, Fluorescence and Magnetism of Two Lanthanide Metal-organic Frameworks with CaF2 Topology Based on Silicon-centered Tetrahedral Ligand

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Chemical Research in Chinese Universities 2013年第4期29卷 611-616页

作者： LI Yang-xue XUE Ming HUANG Lin CHEN Si-ru qiu Shi-lun State Key Laboratory of Inorganic Synthesis and Preparative Chemistry Jilin University Changchun 130012 P. R. China

Two 3D multifunctional lanthanide metal-organic frameworks（MOFs）, Pr（HTCPS）（H20）·2DMF·C2H2OH·5H2O（JUC-93） and Pr3（TCPS）2（NO3）（H2O）4（DMA）2.2DMA.C2H5OH.3H2O（JUC-94）[H4TCPS=tetrakis（4-carboxyphenyl）- silane, DMF=N,N＇-dimethylformamide, DMA=N,N＇-dimethylacetamide and JUC=Jilin University China] were synthe- sized by the self-assembly of a rigid silicon-centered tetrahedral carboxylate ligand H4TCPS and Pr（III） ions in different solvothermal reactions. X-Ray crystallography revealed that they exhibited a rare CaFE topology framework, constructed from the 4-connected tetrahedral TCPS unit with the 8-connected dinuclear praseodymium cluster unit and trinuclear praseodymium cluster unit, respectively. In addition, the luminescent and magnetic properties of the two compounds were investigated.

关键词： Metal-organic framework Tetrakis(4-carboxyphenyl)silane CaF2 topology Magnetic property Fluores- cence

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Possible PKHD1 Hot-spot Mutations Related to Early Kidney Function Failure or Hepatofibrosis in Chinese Children with ARPKD:A Retrospective Single Center Cohort Study and Literature Review

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Current Medical Science 2020年第5期40卷 835-844页

作者： Li-ru qiu Rong-rong XU Jin-hui TANG Jian-hua ZHOU Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

PKHD1 mutations are generally considered to cause autosomal recessive polycystic kidney disease(ARPKD).ARPKD is a rare disorder and one o f the most severe conditions leading to end-stage renal disease in *** the biallelic deletion mutation,patients have difficulty in surviving the perinatal period,resulting in perinatal or neonatal *** study retrospectively analyzed patient characteristics,imaging characteristics,laboratory examinations and family surveys from 7 Chinese children with different PKHD1 gene mutations diagnosed by high-throughput sequencing from January 2014 to February 2018.O f the 7 children,there were 3 males and 4 *** missense mutations,two frameshift mutations,two deletion mutations,and two intronic slicing mutations were *** of the mutations have not previously been *** the literature search,we identified a total of 29 Chinese children with PKHD1 *** missense mutation c.2507T>C in exon 24 was found in one patient in our study,and five patients with liver fibrosis but normal renal function were reported in the *** missense mutation c.5935G>A in exon 37 was found in two patients in our study and three cases in the *** patients had renal failure at an age as young as 1 year of those five patients with the missense mutation c.5935G>A in exon *** was concluded that:(1)Kidney length more than 2-3 SDs above the mean and early-onset hypertension might be associated with PKHDI-associated ARPICD;(2)The more enlarged the kidney size is,the lower the renal function is likely to be;(3)c.5935G>A may be a hot spot that leads to early renal failure in Chinese children with PKHD1 mutations;(4)c.2507T>C may be a hot-spot mutation associated with hepatic lesions in Chinese children with PKHD1.

关键词： PKHD1 mutations autosomal recessive polycystic kidney disease clinical phenotype genotypic characteristics Chinese children

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Syntheses, Structures, Fluorescence and Magnetism of Six Lanthanide Metal-organic Frameworks Based on Silicon-centered Tetrahedral Ligand

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Chemical Research in Chinese Universities 2013年第2期29卷 196-200页

作者： LI Yang-xue XUE Ming GUO Li-jia HUANG Lin CHEN Si-ru qiu Shi-lun State Key Laboratory of Inorganic Synthesis and Preparative Chemistry Jilin University Changchun 130012 P. R. China

Multifunctional lanthanide metal-organic frameworks（MOFs）, M（H4TCPS）（H20）l.5[M=Tb（JUC-95a）, Er（JUC-95b）, Dy（JUC-95c）, Tm（JUC-95d）, Y（JUC-95e） and Pr（JUC-95f）; H4TCPS=tetrakis（4-carboxyphenyl）silane] were synthesized via the reaction of the lanthanide metal ions（Ln3） with a rigid silicon-centered tetrahedral carbo- xylate ligand H4TCPS via a hydrothermal synthesis method. X-Ray diffraction（XRD） analyses reveal that they are extremely similar in structure and crystallized in a monoclinic system with space group C2/c. Two eight-coordinated metal centers and four tetrahedral H4TCPS groups constructed a paddle-wheel building block. The paddle-wheel buil- ding blocks assembled with each other via one oxygen bridge from a water molecule to lead to a 1D infinite inorganic rod-shaped chain, --Y--O--C--O--Y--, along the [001] direction. These 1D inorganic rod-shaped chains linked with the phenyl groups of the tetrahedral HaTCPS ligand to form a 3D framework. In addition, the luminescent and magnetic properties of these compounds show that they could be potential antiferromagnetic and fluorescent mate- rials.

关键词： Metal-organic framework Tetrakis(4-carboxyphenyl)silane Magnetic property Fluorescence

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Relationship between Vesicoureteral Reflux and Glomerular Filtration Rate in Children

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Current Medical Science 2020年第5期40卷 845-850页

作者： Wei ZHANG Hui-ming YI Xiao-le ZHANG Yong-hong YI Jian-hua ZHOU Li-ru qiu Department of Ultrasound Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Vesicoureteral reflux(VUR)is one of the most common urinary tract anomalies in children and causes renal damage and studies focusing on the effect of VUR on renal function are *** recruited 35 primary VUR patients with recurrent urinary tract infection(UTI)and 10 non-VUR patients with recurrent ***-enhanced voiding urosonography(ceVUS)was performed for VUR grading,and renal dynamic imaging was used for evaluating glomerular filtration rate(GFR,mL/min).Standardized GFR(sGFR),namely GFR/BSA(mL·min-1·m-2),was calculated based on the body surface area(BSA).Total sGFR(tsGFR,mL·min-1·m-2)was obtained from the sum of sGFR on the left and right sides of all the *** risk of renal regurgitation was equal in the unilateral reflux *** sGFR of children with grade Ⅳ(45.74±18.05mL·min-1·m-2)and grade V(49.67±23.63mL·min-1·m-2)reflux was significantly lower than that in children with grade Ⅱ(77.69±22.21 mL·min-1·m-2).The renal function compensation of contralateral non-reflux kidney increased in unilateral reflux group,which was higher than that in the control group and level Ⅱ,Ⅳ and Ⅴ of reflux group *** VUR group of the same grade,sGFR decreased with the age at *** unilateral grade V refux group,the tsGFR was lower than that in the unilateral grade I reflux group(133.51±48.21 vs.186.87+53.49mL·min-1·m-2).The patients with VUR of unilateral grade Ⅱ were significantly older than those with VUR of unilateral grades Ⅱ and Ⅳ.This study indicates that severe VUR is significantly associated with decreased renal ***,VUR should be diagnosed early and managed individually.

关键词： vesicoureteral reflux renal dynamic imaging glomerular filtration rate renal damage children

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Overview of ectopic pancreas

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World Journal of Gastrointestinal Surgery 2024年第2期16卷 284-288页

作者： Chang-Fei Li qiu-ru Li Miao Bai Yuan-Shi Lv Yan Jiao Patient Service Center The Second Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina Department of Neurology The Second Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina Department of Fundus Disease The Second Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina Department of Thyroid Head and Neck Surgery Jilin Cancer HospitalChangchun 130000Jilin ProvinceChina Department of Hepatobiliary and Pancreatic Surgery General Surgery CenterThe First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

This editorial discusses the article written by Zheng et al that was published in the latest edition of the World Journal of Gastrointestinal *** primary focus is on the causes,location,diagnosis,histological classification,and therapy of ectopic *** pancreas refers to the presence of pancreatic tissue that is situated in a location outside its usual anatomical placement,and is not connected to the normal pancreas in terms of blood supply or anatomical ***,the embryological origin of ectopic pancreas remains *** most prevalent form of ectopic pancreatic is gastric ectopic *** ultrasonography examination can visualize the morphological charac-teristics of the ectopic pancreatic lesion and pinpoint its anatomical *** histological categorization of ectopic pancreas *** treatment has been widely advocated in ectopic pancreas.

关键词： Pancreas Gastric ectopic pancreas Diagnosis Treatment Anatomy

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Hoist the sails,promote the hopes

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Chinese Medical Journal 2021年第24期134卷 2899-2900页

作者： Li-ru qiu Xiao-Ping Luo Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China

With the combined efforts of the pediatric medical practitioners,children’s health level and pediatric disease treatment standards in China have greatly *** mortality of infants and children under the age of 5 has decreased ***-supportive technology,including newborn care and management of difficult and critical cases,has developed by leaps and *** diagnosis,treatment,and scientific research levels of pediatric subspecialties have become comparable to developed *** have cooperated with international colleagues to write guidelines and expert consensus on a number of pediatric conditions for global *** expertise has a great influence in the field of international pediatrics and has become indispensable and *** Medical Journal,a high-ranking medical journal in China,sends this special issue to compile hot and key issues of pediatrics and publishes 9 articles on basic and clinical research,which are of great significance for the development of pediatrics.

关键词： diagnosis mortality journal

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Palmprint Recognition Based on Statistical Local Binary Orientation Code

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Journal of Electronic Science and Technology 2010年第3期8卷 230-236页

作者： Mei-ru Mu qiu-Qi ruan the Institute of Information Science Beijing Jiaotong University Beijing 100044 China

A novel coding based method named as local binary orientation code （LBOCode） for palmprint recognition is proposed. The palmprint image is firstly convolved with a bank of Gabor filters, and then the orientation information is attained with a winner-take-all rule. Subsequently, the resulting orientation mapping array is operated by uniform local binary pattern. Accordingly, LBOCode image is achieved which contains palmprint orientation information in pixel level. Further we divide the LBOCode image into several equal-size and nonoverlapping regions, and extract the statistical code histogram from each region independently, which builds a global description of palmprint in regional level. In matching stage, the matching score between two palmprints is achieved by calculating the two spatial enhanced histograms＇ dissimilarity, which brings the benefit of computational simplicity. Experimental results demonstrate that the proposed method achieves more promising recognition performance compared with that of several state-of-the-art methods.

关键词： Gabor filter local binary pattern orientation code palmprint recognition.

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Kindergartens Reopening in the Period of Regular Epidemic Prevention and Control, Benefitial or Harmful?

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Current Medical Science 2020年第5期40卷 817-821页

作者： Qiao-qiao DONG Li-ru qiu Li-ming CHENG Sai-nan SHU Yu CHEN Yue ZHAO Yan HAO Huan SHI Xiao-ping LUO Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Laboratory Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

In the period of regular epidemic prevention and control of coronavirus disease 2019(COVID-19)in our country,work resumption has been fully *** there are still new sporadic local cases and imported cases across the *** this situation,whether kindergartens reopening will increase the risk of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spread still remains *** reviewed two pediatric patients with moderate COVID-19,collected the epidemiologic information and monitored the cycle threshold value of rectal specimen and the viral loads,and discussed the transmission of SARS-CoV-2 in pediatric patients and the virulence of feces in children with moderate COVID-19,in order to analyze the risk of kindergartens reopening.

关键词： COVID-19 SARS-CoV-2 transmission children viral load

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引黄工程运行控制系统仿真

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系统仿真学报 2001年第1期13卷 56-59页

作者：何秋茹航天机电集团北京仿真中心北京100854

引黄工程运行控制系统仿真是引黄工程全系统运行计算机仿真项目的重要组成部分之一。本文简要描述了引黄工程运行控制问题的特点和运行控制系统设计的目标，在此基础上，介绍了该控制系统分析方法、控制方案、仿真模型、以及典型工况仿... 详细信息

引黄工程运行控制系统仿真是引黄工程全系统运行计算机仿真项目的重要组成部分之一。本文简要描述了引黄工程运行控制问题的特点和运行控制系统设计的目标，在此基础上，介绍了该控制系统分析方法、控制方案、仿真模型、以及典型工况仿真的基本结论。

关键词：引黄工程运行控制系统系统仿真计算机仿真

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