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Cytotoxicity and Apoptosis Induction in Human HepG2 Hepatoma Cells by Decabromodiphenyl Ethane

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Biomedical and Environmental Sciences 2012年第5期25卷 495-501页

作者： SUN ru bao XI Zhu Ge YAN Jun YANG Hong Lian Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety Tianjin Institute of Health and Environmental MedicineTianjin 300050China Beijing Institute of Disease Control and Prevention Beijing 100071China

Abstract Objective To investigate the toxic effects of decabromodiphenyl ethane （DBDPE）, used as an alternative to decabromodiphenyl ether in vitro. Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations （3.125-100.0 mg/L） for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied. Results As evaluated by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a time- and dose-dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5-100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species （ROS）. Furthermore, N-acetyI-L-cysteine, a widely used ROS scavenger, significantly reduced DBDPE-induced ROS levels and increased HepG2 cells viability. Conclusion DBDPE has cytotoxic and anti-proliferation effect and can induce apoptosis in which ROS plays an important role

关键词： Apoptosis Cytotoxicity Decabromodiphenyl ethane Flame retardants Reactive oxygenspecies

The m6A reader MhYTP2 regulates the stability of its target mRNAs contributing to low nitrogen tolerance in apple (Malus domestica)

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Horticulture Research 2023年第6期10卷 287-298页

作者： Tianli Guo Zehua Yang ru bao Xiaomin Fu Na Wang Changhai Liu Fengwang Ma State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China

Studies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization,splicing,degradation,translation,and other metabolic ***,we discovered that the apple(Malus domestica)m6A reader MhYTP2 bound with and destabilized m6A-modified MdMLO19 *** addition,it enhanced the translation efficiency of m6A-modified mRNA of MdGDH1L,encoding a glutamate dehydrogenase,which confers resistance to powdery *** this study,we report the function of MhYTP2 in the regulation of resistance to low nitrogen(N).The overexpression of MhYTP2 enhances the resistance of apple to low *** show that MhYTP2 binds with and stabilizes the mRNAs of MdALN,which participates in the allantoin catabolic process and cellular response to N starvation in apple;MdPIDL,which participates in root hair elongation;MdTTG1,which is involved in the differentiation process of trichomes;and MdATG8A,which is a core participant in the regulation of *** addition,MhYTP2 accelerates the degradation of MdRHD3 mRNA,which regulates root *** immunoprecipitation-seq and electrophoretic mobility shift assays show that the mRNAs of MdALN,MdATG8A,MdPIDL,MdTTG1,and MdRHD3 are the direct targets of *** or knocking down the above genes in MhYTP2 overexpressing plants dismisses the function of MhYTP2 under low N,suggesting the role of MhYTP2 is dependent on those ***,these results demonstrate that MhYTP2 enhances the resistance of apple to N deficiency by affecting the stability of the bound mRNAs.

关键词： dis translation expressing

Subacute Effect of Decabromodiphenyl Ethane on Hepatotoxicity and Hepatic Enzyme Activity in Rats

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Biomedical and Environmental Sciences 2014年第2期27卷 122-125页

作者： SUN ru bao XI Zhu Ge ZHANG Hua Shan ZHANG Wei Beijing Institute of Disease Control and Prevention Tianjin Institute of Health and Environmental Medicine Tianjin Key Laboratory of Risk Assessment and Control for Environmental and Food Safety

Information regarding decabromodiphenyl ethane （DBDPE） effects on hepatotoxicity and metabolism is limited. In the present study, Wistar rats were given oral DBDPE at different doses. DBDPE induced oxidative stress, elevated blood glucose levels, increased CYP2B2 mRNA, CYP2B1/2 protein, 7-pentoxyresorufin O-depentylase （PROD） activity, and induced CYP3A2 mRNA, CYP3A2 protein, and luciferin benzylether debenzylase （LBD） activity. UDPGT activity increased with its increasing exposure levels, suggesting that oral DBDPE exposure induces drug-metabolizing enzymes in rats via the CAR/PXR signaling pathway. The induction of CYPs and co-regulated enzymes of phase II biotransformation may affect the homeostasis of endogenous substrates, including thyroid hormones, which may, in turn, alter glucose metabolism.

关键词： DBDPE Subacute Effect of Decabromodiphenyl Ethane on Hepatotoxicity and Hepatic Enzyme Activity in Rats CYP PROD LBD Figure

Exposure Assessment of Sb2O3 in PET Food Contact Materials

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Biomedical and Environmental Sciences 2016年第4期29卷 305-313页

作者： ZHU Lei WANG Zhu Tian XU Hai Bin SUN ru bao ZHANG Hong ZHANG Jian Bo China National Centre for Food Safety Risk Assessment Beijing 100022 China Institute of Disease Control and Prevention Academy of Military Medical Science Beijing 100071 China

This study was conducted to do exposure assessment of the possible migration of antimony trioxide （Sb203） from Polyethylene terephthalate （PET） food contact materials （FCM）. Consumption Factor （CF） and Food-type Distribution Factor （fT） were calculated from survey data with reference to the US FDA method. The most conservative migration conditions were obtained by testing Sb migration from PET FCM based on the Chinese national standard of GB/T 5009.101-2003[1].

关键词： PET Exposure Assessment of Sb2O3 in PET Food Contact Materials FCM Sb

Fast rate （≥ 250 beats/min） right ventricular burst stimulation is useful for ventricular tachycardia induction in arrhythmogenic right ventricular cardiomyopathy

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Journal of Geriatric Cardiology 2016年第1期13卷 70-74页

作者： Ling-Min WU Jing-ru bao Yan YAO Bing-Bo HOU Li-Hui ZHENG Shu ZHANG State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Cardiovascular Center PLA Navy General Hospital Beijing China

Background One of the major challenges in arrhythmogenic right ventricular cardiomyopathy （ARVC） ablation is ventricular tachy-cardia （VT） non-inducibility. The study aimed to assess whether fast rate （≥ 250 beats/min） right ventricular burst stimulation was useful for VT induction in patients with *** Ninety-one consecutive ARVC patients with clinical sustained VT that underwent electro-physiological study were enrolled. The stimulation protocol was implemented at both right ventricular apex and outflow tract as follows： Step A, up to double extra-stimuli; Step B, incremental stimulation with low rate （＆lt; 250 beats/min）; Step C, burst stimulation with fast rate （≥ 250 beats/min）; Step D, repeated all steps above with intravenous infusion of *** A total of 76 patients had inducible VT （83.5%）, among which 49 were induced by Step C, 15 were induced by Step B, 8 and 4 by Step A and D, respectively. Clinical VTs were induced in 60 patients （65.9%）. Only two spontaneously ceased ventricular fibrillations were induced by Step C. Multivariate analysis showed that a narrower baseline QRS duration under sinus rhythm was independently associated with VT non-inducibility （OR： 1.1; 95% CI： 1.0-1.1;P = 0.019）.ConclusionFast rate （≥ 250 beats/min） right ventricular burst stimulation provides a useful supplemental method for VT induction in ARVC patients.

关键词： Burst stimulation Cardiomyopathy Right ventricular Ventricular tachycardia

Light Emission and Dynamic Failure Mechanism of Hypervelocity Impact on Zr-Ti-Ni-Cu-Be Bulk Metallic Glass

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Chinese Physics Letters 2011年第9期28卷 201-204页

作者： SUN bao-ru ZHAN Zai-Ji LIANG Bo ZHANG rui-Jun WANG Wen-Kui State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004

Hypervelocity impact on rectangular plate-shaped Zr41Ti14Cu12.5Ni10Be22.5 bulk metallic glass(BMG)is performed by a two−stage light gas *** targets used in the experiment are BMG plates with a thickness of 5 *** projectile,spherical aluminum(3.1 mm in diameter),is accelerated up to various velocities;the light is detected with a *** emission lasts from 200µs up to 1500µs and the intensity increases from 44 to 900 W/(Sr⋅µm).The duration and intensity of a light emission seem to depend on the impact velocity and the extent of target destruction through the formation of impact craters or penetration.

关键词： Impact impact shaped

Influence of Iron Supplementation on DMT1(IRE)-induced Transport of Lead by Brain Barrier Systems in vivo

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Biomedical and Environmental Sciences 2015年第9期28卷 651-659页

作者： AN Dai Zhi AI Jun Tao FANG Hong Juan SUN ru bao SHI Yun WANG Li Li WANG Qiang Institute of Disease Control and Prevention Academy of Military Medical Sciences Beijing Vocational College of Agriculture Beijing Tian Tan Hospital Capital Medical University

Objective To investigate the potential involvement of DMT1（IRE） protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1（IRE） protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1（IRE） m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1（IRE）. Conclusion These results suggest that Pb is transported into the brain through DMT1（IRE）, and the ERK MAPK pathway is involved in DMT1（IRE）-mediated transport regulation in brain vascular system in vivo.

关键词： Lead Iron Blood-brain barrier Divalent metal transporter 1 MAPK pathway

Research on Overall Planning and Implementation Evaluation of Land Use of Xinjiang Production and Construction Corps Agriculture-seven Division

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Asian Agricultural Research 2013年第2期5卷 39-44页

作者： ru bao Minhua GAO Xiaolong LI The Oasis Ecological Key Laboratory School of Resource and Environmental Science Xinjiang University

Based on methods for evaluating the overall planning and implementation of land use of domestic scholars, a comprehensive and highly-operable index system and evaluating method for land use planning multi-type implementation has been established through selecting multi-type evaluating indicators and applied to the last-round overall planning of land use in Xinjiang Production and Construction Corps Agriculture-seven Division. The obtained comprehensive score was 86.40, indicating that land use overall planning has been excellently coordinated with land, society, economy as well as ecology and has exerted outstanding effect with the rank as "good". The evaluation results are in accordance with actual conditions, which displays that the established multi-type implementation evaluating system of land use overall planning is reasonable with scientific evaluating methods and can be the reference for evaluation of land use overall planning in other regiments and companies of Xinjiang Production and Construction Corps.

关键词： Xinjiang Production and Construction Corps Agricul

Oxidative Damage to Lung Tissue and Peripheral Blood in Endotracheal PM_(2.5)-treated Rats

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Biomedical and Environmental Sciences 2009年第3期22卷 223-228页

作者： ZHI-QING LIN ZHU-GE XI DAN-FENG YANG Fu-HUAN CHAO HUA-SHAN ZHANG WEI ZHANG HUANG-LIANG LIU ZAI-MING YANG ru-bao SUN Institute of Health and Environmental Medicine Tianjin 300050 China State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control Tianjin 300071 China

Objective To investigate the oxidative damage to lung tissue and peripherial blood in PM2.5-treated rats. Methods PM2.5 samples were collected using an auto-sampling instrument in summer and winter. Treated samples were endotracheally instilled into rats. Activity of reduced glutathione peroxidase （GSH-Px） and concentration of malondialdehyde （MDA） were used as oxidative damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. DNA migration length （μm） and rate of tail were used as DNA damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. Results The activity of GSH-Px and the concentration of MDA in lung tissue significantly decreased after exposure to PM2.5 for 7-14 days. In peripheral blood, the concentration of MDA decreased, but the activity of GSH-Px increased 7 and 14 days after experiments. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. The DNA migration length （μm） and rate of tail in lung tissue and peripheral blood significantly increased 7 and 14 days after exposure to PM2.5. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. Conclusion PM2.5 has a definite oxidative effect on lung tissue and peripheral blood. The activity of GSH-Px and the concentration of MDA are valuable biomarkers of oxidative lung tissue damage induced by PM2.5. The DNA migration length （μm） and rate of tail are simple and valuable biomarkers of PM2 5-induced DNA damage in lung tissues and peripheral blood. The degree of DNA damage in peripheral blood can predict the degree of DNA damage in lung tissue.

关键词： PM2.5 Endotracheal instillation Oxidative damage Oxidative DNA damage Biomarker DNA repair