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Knock-out of BnHva22c reduces the susceptibility of Brassica napus to infection with the fungal pathogen Verticillium longisporum

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The Crop Journal 2024年第2期12卷 503-514页

作者： Wanzhi Ye roxana Hossain Michael prbsting Abdallah Abdelmegid Mohamed Ali Lingyue Han Ying Miao Steffen Rietz Daguang Cai Dirk Schenke Department of Molecular phytopathology and Biotechnology Institute of PhytopathologyChristian-Albrechts-University of KielKielGermany Institut für Zuckerrübenforschung GöttingenGermany plant pathology Department Faculty of Agriculture and Natural ResourcesAswan UniversityAswanEgypt Fujian provincial Key Laboratory of plant Functional Biology College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Hohenlieth-Hof NPZ Innovation GmbHHoltseeGermany

Verticillium longisporum(Vl43)is a soilborne hemibiotrophic fungal pathogen causing stem striping on oilseed rape(OSR)and severe yield *** for resistant varieties is the most promising approach to control this ***,we report the identification of Hva22c as a novel susceptibility factor and its potential for improving OSR ***22c is a member of the Hva22 gene family,originally described for barley(Hordeum vulgare).Several Hva22 members have been located at the endoplasmic ***22c is up-regulated in response to Vl43 in both Arabidopsis and *** demonstrate that knock-out of Hva22c in OSR by CRISpR/Cas9 and its homolog in Arabidopsis by T-DNA insertion reduced plants’susceptibility to Vl43 infection and impaired the development of disease *** understand the underlying mechanism,we analysed transcriptomic data from infected and non-infected roots of hva22c knock-out and wild type *** identified a homozygous mutant with frame-shifts in all four BnHva22c loci displaying a vastly altered transcriptional landscape at 6 ***,a large set of genes was suppressed under mock conditions including genes related to the endomembrane *** the up-regulated genes we found several defense-related and phytohormone-responsive genes when comparing mutant to the wild *** results demonstrate that Hva22c is functionally required for a fully compatible plant-fungus *** loss of function reduces plant susceptibility,most likely due to endoplasmatic reticulum and Golgi dysfunction accompanied by additionally activated defense *** findings can help improve OSR resistance to *** infection.

关键词： Hva22c Susceptibility factor CRISpR/Cas9 Brassica napus Arabidopsis Verticillium longisporum Resistance breeding

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prospective study of hepatitis B and D epidemiology and risk factors in Romania:A 10-year update

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World Journal of Hepatology 2024年第4期16卷 640-649页

作者： Speranta Iacob Liana Gheorghe Mirela Onica Laura Huiban Corina Silvia pop Ciprian Brisc roxana Sirli Carmen Ester Cristina Mihaela Brisc Sorina Diaconu Ion Rogoveanu Larisa Sandulescu Deiana Vuletici Anca Trifan Department of Gastroenterology Carol Davila University of Medicine and PharmacyBucharest 050474Romania Department of Gastroenterology and Hepatology Fundeni Clinical InstituteBucharest 022328Romania Department of Gastroenterology Grigore T Popa University of Medicine and PharmacyIasi 700115Romania Institute of Gastroenterology and Hepatology Saint Spiridon County HospitalIasi 700111Romania Department of Gastroenterology and Medical Oncology University Emergency Clinical HospitalBucharest 050098Romania Faculty of Medicine and pharmacy University of OradeaOradea 410087Romania Department of Gastroenterology Emergency County HospitalOradea 410169Romania Center for Advanced Research in Gastroenterology and Hepatology Department of Gastroenterology and Hepatology“Victor Babeş”University of Medicine and PharmacyTimisoara 300041Romania Department of Gastroenterology and Hepatology TimişCounty Emergency Clinical Hospital"Pius Branzeu"Timisoara 300723Romania Department of Internal Medicine II and Gastroenterology University Emergency Clinical HospitalBucharest 050098Romania Department of Gastroenterology University of Medicine and PharmacyCraiova 200349Romania Department of Cardiology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Research Center of Gastroenterology and HepatologyUniversity of Medicine and PharmacyCraiova 200349Romania Department of Gastroenterology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Faculty of MedicineGrigore T Popa University of Medicine and PharmacyIasi 700115Romania

BACKGROUND The global burden of hepatitis D virus(HDV)infection represents a major medical challenge and a public health crisis ***,there is a lack of accurate data on the epidemiology and risk factors for *** B virus(HBV)and HDV coinfection causes the most severe form of viral hepatitis,leading to a higher cumulative incidence of liver-related events compared with HBV monoinfection,including the need for liver transplantation and *** To investigate the epidemiology,natural history,risk factors and clinical management of HBV and HDV coinfection in Romanian *** This prospective study was conducted between January and July 2022 in six tertiary gastroenterology and hepatology referral centres in *** consecutive adults admitted for any gastroenterology diagnosis who were HBV-positive were *** with acute hepatitis or incomplete data were *** the 25390 individuals who presented with any type of gastroenterology diagnosis during the study period,963 met the inclusion *** for anti-HDV antibodies and HDV RNA was performed for all *** and risk factor data were collected by investigators using medical charts and patient *** data were stored in an anonymized online database during the *** The prevalence of HBV was 3.8%;among these patients,the prevalence of HBV/HDV coinfection was 33.1%.The median age of the study population was 54.0 years,and it consisted of 55.1%men.A higher prevalence of HBV/HDV coinfection was observed in patients 50–69 years *** with HBV/HDV coinfection were significantly older than those with HBV monoinfection(p=0.03).Multivariate multiple regression analysis identified female gender(p=0.0006),imprisonment(pp=0.01)and sexual contact with persons with known viral hepatitis(p=0.0003)as significant risk factors for *** This study shows that HDV infection among those with HBV re

关键词： Epidemiology Hepatitis B Hepatitis D Natural history Risk factors Romania

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Soil organic carbon stocks in native forest of Argentina:a useful surrogate for mitigation and conservation planning under climate variability

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ecological processes 2024年第1期13卷 3-24页

作者： pablo L.peri Juan Gaitán Matías Mastrangelo Marcelo Nosetto pablo E.Villagra Ezequiel Balducci Martín pinazo roxana p.ec.esia Alejandra Von Wallis Sebastián Villarino Francisco Alaggia Marina González polo Silvina Manrique pablo A.Meglioli Julián Rodríguez-Souilla Martín Mónaco Jimena E.Chaves Ariel Medina Ignacio Gasparri Eugenio Alvarez Arnesi María paula Barral Axel von Müller Norberto M.pahr Josefina Uribe echevarria pedro Fernández Marina Morsucci Dardo López Juan Manuel Cellini Leandro Alvarez Ignacio Barberis Hernán Colomb Ludmila La Manna Sebastián Barbaro Cecilia Blundo Ximena Sirimarco Laura Cavallero Gualberto Zalazar Guillermo Martínez pastur Instituto Nacional de Tecnología Agropecuaria (INTA) Universidad Nacional de la patagonia Austral (UNpA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Luján Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Grupo de Estudio de Agroecosistemas y paisajes Rurales (GEAp) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) CCT San Luis Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IANIGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Cuyo Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto de Investigaciones en Energía No Convencional CCT Salta-JujuyConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Dirección Nacional de Bosques Ministerio de Ambiente y Desarrollo Sostenible de la Nación Instituto de ecología Regional Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )Universidad Nacional de Tucumán Universidad Nacional de Rosario Instituto de Investigaciones en Ciencias Agrarias de Rosario Centro Científico Tecnológico CONICET Grupo de Estudio de Agroecosistemas y paisajes Rurales (GEAp) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Investigaciones en Maderas (LIMAD) Universidad Nacional de La Plata (UNLP) Administración de parques Nacionales (ApN) Parque Nacional Los Alerces Centro de Estudios Ambientales Integrados (CEAI) Universidad Nacional de la Patagonia San Juan Bosco (UNPASJB) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background The nationally determined contribution（NDC） presented by Argentina within the framework of the paris Agreement is aligned with the decisions made in the context of the United Nations Framework Conven-tion on Climate Change（UNFCCC） on the reduction of emissions derived from deforestation and forest degradation, as well as forest carbon conservation（REDD+）. In addition, climate change constitutes one of the greatest threats to forest biodiversity and ecosystem services. However, the soil organic carbon（SOC） stocks of native forests have not been incorporated into the Forest Reference Emission Levels calculations and for conservation planning under cli-mate variability due to a lack of information. The objectives of this study were:（i） to model SOC stocks to 30 cm of native forests at a national scale using climatic, topographic and vegetation as predictor variables, and（ii） to relate SOC stocks with spatial–temporal remotely sensed indices to determine biodiversity conservation concerns due to threats from high inter-annual climate *** We used 1040 forest soil samples（0–30 cm） to generate spatially explicit estimates of SOC native forests in Argentina at a spatial resolution of approximately 200 m. We selected 52 potential predictive environmental covari-ates, which represent key factors for the spatial distribution of SOC. All covariate maps were uploaded to the Google Earth Engine cloud-based computing platform for subsequent modelling. To determine the biodiversity threats from high inter-annual climate variability, we employed the spatial–temporal satellite-derived indices based on Enhanced Vegetation Index（EVI） and land surface temperature（LST） images from Landsat *** SOC model（0–30 cm depth） prediction accounted for 69% of the variation of this soil property across the whole native forest coverage in Argentina. Total mean SOC stock reached 2.81 pg C（2.71–2.84 pg C with a probability of 90%） for a total area of 460,790 km2,

关键词： Soil organic carbon Biodiversity Native forest Climate change mitigation Modelling Resilience

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The impact of daylight and window views on length of stay among patients with heart disease:A retrospective study in a cardiac intensive care unit

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Journal of Intensive Medicine 2023年第2期3卷 155-164页

作者： roxana Jafarifiroozabadi Anjali Joseph William Bridges Andrea Franks College of Architecture and Design Lawrence Technological UniversitySouthfieldMI 48075USA Spartanburg Regional Healthcare System Endowed Chair in Architecture+Health Design Clemson UniversityClemsonSC 29634USA Center for Health Facilities Design and Testing Clemson UniversityClemsonSC 29634USA School of Architecture Clemson UniversityClemsonSC 29634USA Industrial Engineering Clemson UniversityClemsonSC 29634USA School of Mathematical and Statistical Sciences Clemson UniversityClemsonSC 29634USA Clinical and Nursing Research AnMed HealthAndersonSC 29621USA

Background:Heart disease is the leading cause of death in the United *** length of stay(LOS)is a well-established parameter used to evaluate health outcomes among critically ill patients with heart disease in cardiac intensive care units(CICUs).While evidence suggests that the presence of daylight and window views can positively influence patients’LOS,no studies to date have differentiated the impact of daylight from window views on heart disease ***,existing research studies on the impact of daylight and window views have failed to account for key clinical and demographic variables that can impact the benefit of such interventions in ***:This retrospective study investigated the impact of access to daylight *** views on CICU patients'*** study CICU is located in a hospital in the southeast United States and has rooms of the same size with different types of access to daylight and window views,including rooms with daylight and window views(with the patient bed located parallel to full-height,south-facing windows),rooms with daylight and no window views(with the patient bed located perpendicular to the windows),and windowless *** from electronic health records(EHRs)for the time-period September 2015 to September 2019(n=2936)were analyzed to investigate the impact of room type on patients’CICU *** regression models were developed for the outcome of interest,controlling for potential confounding ***:Ultimately,2319 patients were finally included in the study *** indicated that patients receiving mechanical ventilation in rooms with access to daylight and window views had shorter LOS durations(16.8 h)than those in windowless *** analysis for a subset of patients with LOS≤3 days revealed that parallel bed placement to the windows and providing access to both daylight and window views significantly reduced their LOS compared to windowless rooms in the unit(p=0.007).Also,parallel b

关键词： Heart disease Daylight Window views Length of stay Cardiac intensive care unit

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Intersections between innate immune response and gastric cancer development

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World Journal of Gastroenterology 2023年第15期29卷 2222-2240页

作者： Franz Villarroel-Espindola Troy Ejsmentewicz roxana Gonzalez-Stegmaier Roddy A Jorquera Esteban Salinas Translational Medicine Unit Instituto Oncologico Fundacion Arturo Lopez PerezSantiago 7500000Metropolitan regionChile

Worldwide,gastric cancer(GC)is the fifth most commonly diagnosed *** has a reduced prevalence but has maintained its poor prognosis being the fourth leading cause of deaths related to *** highest mortality rates occur in Asian and Latin American countries,where cases are usually diagnosed at advanced ***,GC is viewed as the consequence of a multifactorial process,involving the virulence of the Helicobacter pylori(***)strains,as well as some environmental factors,dietary habits,and host intrinsic *** tumor microenvironment in GC appears to be chronically inflamed which promotes tumor progression and reduces the therapeutic *** has been suggested that inflammation assessment needs to be measured qualitatively and quantitatively,considering cell-infiltration types,availability of receptors to detect damage and pathogens,and presence or absence of aggressive *** *** epithelial cells express several Toll-like receptors and determine the first defensive line against pathogens,and have been also described as mediators of ***,other molecules,such as cytokines related to inflammation and innate immunity,including immune checkpoint molecules,interferon-gamma pathway and NETosis have been associated with an increased risk of ***,this review will explore innate immune activation in the context of premalignant lesions of the gastric epithelium and established gastric tumors.

关键词： Gastric cancer Toll-like receptor Helicobacter pylori Nuclear factor kappa B Neutrophils

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“Treat-Repair-Treat”:Management of Left Main Coronary Compression by a pulmonary Artery Aneurysm in a patient with Atrial Septal Defect and Significant pulmonary Hypertension

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Congenital Heart Disease 2023年第1期18卷 67-72页

作者： Andrei George Iosifescu roxana Enache Ioana Marinică Corina Radu Vlad Anton Iliescu Carol Davila University of Medicine and pharmacy Bucharest020021Romania Department of Cardiac Surgery C.C.Iliescu Emergency Institute for Cardiovascular DiseasesBucharest022322Romania Department of Cardiology C.C.Iliescu Emergency Institute for Cardiovascular DiseasesBucharest022322Romania Department of Cardiovascular Anesthesia and Intensive Care C.C.Iliescu Emergency Institute for Cardiovascular DiseasesBucharest022322Romania

Left main coronary compression syndrome(LMCS)may complicate pulmonary artery aneurysms(pAA),usually developed in the context of pulmonary arterial hypertension(pAH).We report the case of a 51-year-old female patient with an atrial septal defect(unsuitable for device closure)complicated by a pAA generating a 90%left main *** significant pAH held us back from immediate *** specific dual pAH-targeted therapy(sildenafil and bosentan),the atrial septal defect could be closed with a unidirectional valved patch;the pAAinduced LMCS was treated by reductive *** postoperative course was ***-up showed clinical improvement,but pAH treatment was still *** three months,coronary angiography showed only an insignificant residual left main stenosis,proving that reductive pulmonary arterioplasty was effective in treating *** pAA requires further evaluation for LMCS,a dangerous but treatable ***“treat-repair-treat”strategy and shunt-closure with a unidirectional valved patch can both improve surgical prospects of LMCS with shunt-related pAH.

关键词： Atrial septal defect pulmonary arterial hypertension pulmonary artery aneurysm left main coronary compression syndrome surgery unidirectional valved patch

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Mean-field BSDEs with jumps and dual representation for global risk measures

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probability, Uncertainty and Quantitative Risk 2023年第1期8卷 33-52页

作者： Rui Chen roxana Dumitrescu Andreea Minca Agnès Sulem INRIA paris 2 rue Simone IffCS 4211275589 ParisCedex 12France Universitéparis-Dauphine 75775 ParisCedex 16France Department of Mathematics King’s College LondonStrandLondon WC 2 R 2LSUK Department of Operations Research and Information Engineering Cornell University222 Rhodes HallIthacaNY 14853USA

We study mean-field BSDEs with jumps and a generalized mean-field operator that can capture higher-order *** interpret the BSDE solution as a dynamic risk measure for a representative bank whose risk attitude is influenced by the *** influence can come in a wide class of choices,including the average system state or average intensity of system *** Fenchel−Legendre transforms,our main result is a dual representation for the expectation of the risk measure in the convex *** particular,we exhibit its dependence on the mean-field operator.

关键词： Mean-field interactions BSDEs Dynamic risk measures System influence

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First 100 cases of transvesical single-port robotic radical prostatectomy

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Asian Journal of Urology 2023年第4期10卷 416-422页

作者： roxana Ramos-Carpinteyro Ethan L.Ferguson Jaya S.Chavali Albert Geskin Jihad Kaouk Glickman Urological&Kidney Institute Cleveland Clinic FoundationClevelandOHUSA

Objective:To describe the surgical technique and report the early outcomes of the transvesical(TV)approach to single-port(Sp)robot-assisted radical ***:All procedures were performed at a single center by one *** identified the first 100 consecutive patients with clinically localized prostate cancer that underwent Sp TV robot-assisted radical prostatectomy using the da Vinci Sp robotic surgical *** were collected prospectively and analyzed with descriptive *** primary outcomes assessed were postoperative urinary continence,rate of biochemical recurrence,and sexual ***:All procedures were performed without extra ports or *** median age was 62.1 years and 49.0%of the patients had abdominal surgery *** preoperative median prostate-specific antigen value and prostate volume were 5.0 ng/mL and 33.0 mL,*** were no intraoperative *** median operative time and estimated blood loss were 212.5 min and 100.0 mL,respectively.A total of 92.0%of patients were discharged within 24.0 h,with an overall median length of stay of 5.6 *** 4.0%of patients required opioid prescriptions at *** median Foley catheter duration was 3 *** margins were present in 15.0%of *** follow-up was 10.4 *** rate was immediate after Foley removal in 49.0%of cases,65.0%at 2 weeks,77.4%at 6 weeks,94.1%at 6 months,and 98.9%at 1 *** case of biochemical recurrence(1.0%)was noted 3 months after ***:The Sp TV approach for radical prostatectomy cases is a safe and feasible technique for patients with clinically localized prostate *** technique offers advantages of short hospital stay,minimal narcotic use postoperatively,and promising early return of urinary continence,without compromising oncologic outcomes.

关键词： prostate cancer Radical prostatectomy Robotic-assisted surgery Single-port Minimally-invasive surgery

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Genus pronephrium presl 1851 (Thelypteridaceae) in Romania

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Journal of palaeogeography 2018年第4期7卷 295-307页

作者： roxana pirnea Mihai Emilian popa faculty of geology and geophysics doctoral school of geology university of bucharest 1 N. Ealcescu Ave 010041 BucharestRomania faculty of geology and geophysics department of geology laboratory of palaeontology university of bucharest 1 N. Ealcescu Ave 010041 BucharestRomania

pronephrium stiriacum（Unger） Knobloch et Kvatek 1976（Filicales, Thelypteridaceae Holttum 1971） is reported from the Oligocene petrosani Basin and from the Miocene Bozovici Basin in Romania. Morphological variations of this species, such as size of the leaves, venation and marginal teeth, in the Oligocene-Miocene time interval in Romania are described, discussed and illustrated. The distribution of the Thelypteridacean species pronephrium stiriacum is also discussed regarding its European expansion during the Cenozoic. Moreover, the palaeoecology of this species is discussed based on taphonomy and occurrences, as pronephrium stiriacum was a secondary coal generator.

关键词： Thelypteridaceae pronephrium stiriacum Oligocene Miocene Romania

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State of the art contemporary treatment of patients with ST elevationmyocardial infarction： pre- and in-hospital organization, devices anddrugs

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Chinese Medical Journal 2014年第6期127卷 1133-1140页

作者： Mikkel Malby Schoos roxana Mehran The Zena and Michael A.Wiener Cardiovascular Institute The Icahn School of Medicine at Mount Sinai New York NY 10029-6574USA

Objective To review the presentation,diagnosis and recent developments in the pharmacological and invasive treatment of ST elevation myocardial infarction (STEMI) with a special focus on health-care organization in order to increase accessibility of primary pemutaneous coronary intervention (pCI).Data sources Data were obtained from English publications on STEMI *** formal systematic review was conducted,but an effort was made to be *** selection Studies were selected if they contained data relevant to the ***,data from clinical randomized trials,meta-analyses,guidelines and a few recent reviews are *** The described clinical approach to acute myocardial infarction (AMI) has been a continuum of scientific results and translation into clinical practice over the last four decades since the advent of thrombolytic *** has resulted in a dramatic in-hospital mortality decrease from 30% in the 1960s to the present 5%.The biggest survival benefits have undoubtedly been achieved after the advent of reperfusion *** contemporary treatment of STEMI,additional treatment effects on survival have to be sought in the very early admission phase,as the current mortality hazard drops significantly after the first critical days to continuously very low levels after *** Optimal treatment of STEMI patients is best performed with a widely accessible reperfusion strategy,preferably primary pCI,with contemporary peri-procedural anti-thrombotic treatment and device *** of reperfusion strategies is increased by efficient STEMI networks applying prehospital triage with digital tele-transmission of electrocardiograms (ecGs) and seamless patient transitions between health-care *** treatments of complicated STEMI with out-of hospital cardiac arrest and/or cardiogenic shock underline the necessity of structured referral systems,preferably immediately after the initial

关键词： acute myocardial infarction ST elevation myocardial infarction primary percutaneous coronary intervention fibrinolysis pre-hospital triage antiplatelet therapy antithrombotic therapy drug eluting stents cardiogenic shock out-ofhospital cardiac arrest

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