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Knock-out of BnHva22c reduces the susceptibility of Brassica napus to infection with the fungal pathogen Verticillium longisporum

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The Crop Journal 2024年第2期12卷 503-514页

作者： Wanzhi Ye roxana Hossain Michael Prbsting Abdallah Abdelmegid Mohamed Ali Lingyue Han Ying Miao Steffen Rietz Daguang Cai Dirk Schenke Department of Molecular Phytopathology and Biotechnology Institute of PhytopathologyChristian-Albrechts-University of KielKielGermany Institut für Zuckerrübenforschung GöttingenGermany Plant Pathology Department Faculty of Agriculture and Natural ResourcesAswan UniversityAswanEgypt Fujian Provincial Key Laboratory of Plant Functional Biology College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Hohenlieth-Hof NPZ Innovation GmbHHoltseeGermany

Verticillium longisporum(Vl43)is a soilborne hemibiotrophic fungal pathogen causing stem striping on oilseed rape(OSR)and severe yield *** for resistant varieties is the most promising approach to control this ***,we report the identification of Hva22c as a novel susceptibility factor and its potential for improving OSR ***22c is a member of the Hva22 gene family,originally described for barley(Hordeum vulgare).Several Hva22 members have been located at the endoplasmic ***22c is up-regulated in response to Vl43 in both Arabidopsis and *** demonstrate that knock-out of Hva22c in OSR by CRISPR/Cas9 and its homolog in Arabidopsis by T-DNA insertion reduced plants’susceptibility to Vl43 infection and impaired the development of disease *** understand the underlying mechanism,we analysed transcriptomic data from infected and non-infected roots of hva22c knock-out and wild type *** identified a homozygous mutant with frame-shifts in all four BnHva22c loci displaying a vastly altered transcriptional landscape at 6 ***,a large set of genes was suppressed under mock conditions including genes related to the endomembrane *** the up-regulated genes we found several defense-related and phytohormone-responsive genes when comparing mutant to the wild *** results demonstrate that Hva22c is functionally required for a fully compatible plant-fungus *** loss of function reduces plant susceptibility,most likely due to endoplasmatic reticulum and Golgi dysfunction accompanied by additionally activated defense *** findings can help improve OSR resistance to *** infection.

关键词： Hva22c Susceptibility factor CRISPR/Cas9 Brassica napus Arabidopsis Verticillium longisporum Resistance breeding

Prospective study of hepatitis B and D epidemiology and risk factors in Romania:A 10-year update

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World Journal of Hepatology 2024年第4期16卷 640-649页

作者： Speranta Iacob Liana Gheorghe Mirela Onica Laura Huiban Corina Silvia Pop Ciprian Brisc roxana Sirli Carmen Ester Cristina Mihaela Brisc Sorina Diaconu Ion Rogoveanu Larisa Sandulescu Deiana Vuletici Anca Trifan Department of Gastroenterology Carol Davila University of Medicine and PharmacyBucharest 050474Romania Department of Gastroenterology and Hepatology Fundeni Clinical InstituteBucharest 022328Romania Department of Gastroenterology Grigore T Popa University of Medicine and PharmacyIasi 700115Romania Institute of Gastroenterology and Hepatology Saint Spiridon County HospitalIasi 700111Romania Department of Gastroenterology and Medical Oncology University Emergency Clinical HospitalBucharest 050098Romania Faculty of Medicine and Pharmacy University of OradeaOradea 410087Romania Department of Gastroenterology Emergency County HospitalOradea 410169Romania Center for Advanced Research in Gastroenterology and Hepatology Department of Gastroenterology and Hepatology“Victor Babeş”University of Medicine and PharmacyTimisoara 300041Romania Department of Gastroenterology and Hepatology TimişCounty Emergency Clinical Hospital"Pius Branzeu"Timisoara 300723Romania Department of Internal Medicine II and Gastroenterology University Emergency Clinical HospitalBucharest 050098Romania Department of Gastroenterology University of Medicine and PharmacyCraiova 200349Romania Department of Cardiology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Research Center of Gastroenterology and HepatologyUniversity of Medicine and PharmacyCraiova 200349Romania Department of Gastroenterology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Faculty of MedicineGrigore T Popa University of Medicine and PharmacyIasi 700115Romania

BACKGROUND The global burden of hepatitis D virus(HDV)infection represents a major medical challenge and a public health crisis ***,there is a lack of accurate data on the epidemiology and risk factors for *** B virus(HBV)and HDV coinfection causes the most severe form of viral hepatitis,leading to a higher cumulative incidence of liver-related events compared with HBV monoinfection,including the need for liver transplantation and *** To investigate the epidemiology,natural history,risk factors and clinical management of HBV and HDV coinfection in Romanian *** This prospective study was conducted between January and July 2022 in six tertiary gastroenterology and hepatology referral centres in *** consecutive adults admitted for any gastroenterology diagnosis who were HBV-positive were *** with acute hepatitis or incomplete data were *** the 25390 individuals who presented with any type of gastroenterology diagnosis during the study period,963 met the inclusion *** for anti-HDV antibodies and HDV RNA was performed for all *** and risk factor data were collected by investigators using medical charts and patient *** data were stored in an anonymized online database during the *** The prevalence of HBV was 3.8%;among these patients,the prevalence of HBV/HDV coinfection was 33.1%.The median age of the study population was 54.0 years,and it consisted of 55.1%men.A higher prevalence of HBV/HDV coinfection was observed in patients 50–69 years *** with HBV/HDV coinfection were significantly older than those with HBV monoinfection(P=0.03).Multivariate multiple regression analysis identified female gender(P=0.0006),imprisonment(P<0.0001),older age at diagnosis(P=0.01)and sexual contact with persons with known viral hepatitis(P=0.0003)as significant risk factors for *** This study shows that HDV infection among those with HBV re

关键词： Epidemiology Hepatitis B Hepatitis D Natural history Risk factors Romania

Genus Pronephrium Presl 1851 (Thelypteridaceae) in Romania

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Journal of Palaeogeography 2018年第4期7卷 295-307页

作者： roxana Pirnea Mihai Emilian Popa faculty of geology and geophysics doctoral school of geology university of bucharest 1 N. Ealcescu Ave 010041 BucharestRomania faculty of geology and geophysics department of geology laboratory of palaeontology university of bucharest 1 N. Ealcescu Ave 010041 BucharestRomania

Pronephrium stiriacum（Unger） Knobloch et Kvatek 1976（Filicales, Thelypteridaceae Holttum 1971） is reported from the Oligocene Petrosani Basin and from the Miocene Bozovici Basin in Romania. Morphological variations of this species, such as size of the leaves, venation and marginal teeth, in the Oligocene-Miocene time interval in Romania are described, discussed and illustrated. The distribution of the Thelypteridacean species Pronephrium stiriacum is also discussed regarding its European expansion during the Cenozoic. Moreover, the palaeoecology of this species is discussed based on taphonomy and occurrences, as Pronephrium stiriacum was a secondary coal generator.

关键词： Thelypteridaceae Pronephrium stiriacum Oligocene Miocene Romania

Why,who and how should perform liver biopsy in chronic liver diseases

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World Journal of Gastroenterology 2008年第21期14卷 3396-3402页

作者： Ioan Sporea Alina Popescu roxana Sirli Department of Gastroenterology University of Medicine and PharmacyTimisoara 700736Romania

Chronic viral hepatitis is a common disease in the general *** chronic hepatitis,the prognosis and clinical management are highly dependent on the extent of liver *** fibrosis evaluation can be performed by FibroTest(using serological markers),by Elastography or FibroScan(a noninvasive percutaneous technique using the elastic properties of the hepatic tissue) and by liver biopsy(LB),considered to be the "gold standard".Currently,there are three techniques for performing LB:percutaneous,transjugular and *** percutaneous LB can be performed blind,ultrasound(US) guided or US *** are two main categories of specialists who perform LB:gastroenterologists(hepatologists) and radiologists,and the specialty of the individual who performs the LB determines if the LB is performed under ultrasound guidance or *** are two types of biopsy needles used for LB:cutting needles(Tru-Cut,Vim-Silverman) and suction needles(Menghini,Klatzkin,Jamshidi).The rate of major complications after percutaneous LB ranges from 0.09% to 2.3%,but the echo-guided percutaneous liver biopsy is a safe method for the diagnosis of chronic diffuse hepatitis(cost-effective as compared to blind biopsy) and the rate of complications seems to be related to the experience of the physician and the type of the needle used(Menghini type needle seems to be safer).Maybe,in a few years we will use non-invasive markers of fibrosis,but at this time,most authorities in the field consider that the LB is useful and necessary for the evaluation of chronic hepatopathies,despite the fact that it is not a perfect test.

关键词： Chronic hepatitis Liver biopsy Fibrosis Ultrasound guidance

Biomimetic Decarboxylation of Carboxylic Acids with PhI(OAc)_2 Catalyzed by Manganese Porphyrin [Mn(TPP)OAc]

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Chemical Research in Chinese Universities 2008年第4期24卷 464-468页

作者： GHOLAM REZA Karimipour roxana Ahmadpour Department of Chemistry Islamic Azad University

Manganese（Ⅲ） meso-tetraphenylporphyrin acetate [Mn（TPP）OAc] served as an effective catalyst for the oxidative decarboxylation of carboxylic acids with （diacetoxyiodo）benzene [PhI（OAc）2] in CH2CI2-H2O（95：5, volume ratio）. The aryl substituted acetic acids are more reactive than the less electron rich linear carboxylic acids in the presence of catalyst Mn（TPP）OAc. In the former case, the formation of carbonyl products was complete within just a few minutes with 〉97% selectivities, and no further oxidation of the produced aldehydes was achieved under these catalytic conditions. This method provides a benign procedure owing to the utilization of low toxic（diacetoxyiodo） benzene, biologically relevant manganese porphyrins, and carboxylic acids.

关键词： Manganese porphyrin (Diacetoxyiodo)benzene Carboxylic acid Decarboxylation

Neuroprotective effect of immunomodulatory peptides in rats with traumatic spinal cord injury

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Neural Regeneration Research 2021年第7期16卷 1273-1280页

作者： Dulce Parra-Villamar Liliana Blancas-Espinoza Elisa Garcia-Vences Juan Herrera-García Adrian Flores-Romero Alberto Toscano-Zapien Jonathan Vilchis Villa Rodríguez Barrera-roxana Soria Zavala Karla Antonio ibarra Raúl Silva-García Unidad de Investigación Médica en Inmunología Hospital de PediatríaCentro Médico Nacional Siglo XXI/Instituto Mexicano del Seguro Social/Ciudad de MéxicoMéxico Centro de Investigación en Ciencias de la Salud(CICSA) Facultad de Ciencias de la SaludUniversidad Anáhuac MéxicoCampus NorteHuixquilucanEdo de MéxicoMéxico Centro de Investigación del Proyecto Camina A.C Ciudad de MéxicoMéxico

Several therapies have shown obvious effects on structural conservation contributing to motor functional recovery after spinal cord injury(SCI).Nevertheless, neither strategy has achieved a convincing *** purposed a combined therapy of immunomodulatory peptides that individually have shown significant effects on motor functional recovery in rats with *** objective of this study was to investigate the effects of the combined therapy of monocyte locomotion inhibitor factor(MLIF), A91 peptide, and glutathione monoethyl ester(GSH-MEE) on chronic-stage spinal cord *** Sprague-Dawley rats underwent a laminectomy of the T9 vertebra and a moderate *** groups were included: sham, PBS, MLIF + A91, MLIF + GSH-MEE, A91 + GSH-MEE, and MLIF + A91 + *** months after injury, motor functional recovery was evaluated using the open field *** and white matter preservation was evaluated using hematoxylin & eosin staining and Luxol Fast Blue staining, *** number of motoneurons in the ventral horn and the number of axonal fibers were determined using hematoxylin & eosin staining and immunohistochemistry, *** deposition was evaluated using Masson's trichrome *** combined therapy of MLIF, A91, and GSH-MEE greatly contributed to motor functional recovery and preservation of the medullary parenchyma, white matter, motoneurons, and axonal fibres, and reduced the deposition of collagen in the lesioned *** combined therapy of MLIF, A91, and GSH-MEE preserved spinal cord tissue integrity and promoted motor functional recovery of rats after *** study was approved by the National Commission for Scientific Research on Bioethics and Biosafety of the Instituto Mexicano del Seguro Social under registration number R-2015-785-116(approval date November 30, 2015) and R-2017-3603-33(approval date June 5, 2017).

关键词： glutathione monoethyl ester monocyte locomotion inhibitor factor motor functional recovery neuroprotection neurorestoration peptides protective autoimmunity spinal cord injury

Use of a combination strategy to improve neuroprotection and neuroregeneration in a rat model of acute spinal cord injury

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Neural Regeneration Research 2019年第6期14卷 1060-1068页

作者： Elisa García roxana Rodríguez-Barrera Vinnitsa Buzoianu-Anguiano Adrian Flores-Romero Emanuel Malagón-Axotla Marco Guerrero-Godinez Estefanía De la Cruz-Castillo Laura Castillo-Carvajal Monserrat Rivas-Gonzalez Paola Santiago-Tovar Ivis Morales Cesar Borlongan Antonio ibarra Centro de Investigación en Ciencias de la Salud (CICSA) Facultad de Ciencias de la SaludUniversidad Anáhuac México Campus Norte Centro de Investigación del Proyecto CAMINA A.C. Unidad de Investigación Médica en Enfermedades Neurologicas Hospital Especialidades CMN Siglo XXI Unidad de Rehabilitación Osteoarticular.Instituto Nacional de Rehabilitación. Luis Guillermo ibarra ibarra Center of Excellence for Aging and Brain Repair Department of Neurosurgery and Brain RepairUniversity of South Florida Morsani College of Medicine

Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides(INDP), inhibition of glial scar formation(dipyridyl: DPY), as well as the use of biocompatible matrix(fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells(MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) INDP + DPY; 4) DPY+ FG; 5) INDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord(hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy(DPY+ INDP + FG+ MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy(DPY+ INDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee(approval No. 178544; CSNBTBIBAJ 090812960)on August 15, 2016.

关键词： fibrin glue mesenchymal stem cells glial scar protective autoimmunity neural derived peptides neural regeneration

Black Hole and Sink Hole Attack Detection in Wireless Body Area Networks

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Computers, Materials & Continua 2021年第8期68卷 1949-1965页

作者： Rajesh Kumar Dhanaraj Lalitha Krishnasamy Oana Geman Diana roxana Izdrui School of Computing Science and Engineering Galgotias University201310India Department of Information Technology Kongu Engineering CollegeErode638060India Department of Health and Human Development Stefan cel Mare UniversityRomania Department of Computers Electronics and AutomationStefan cel Mare UniversityRomania

In Wireless Body Area Networks(WBANs)with respect to health care,sensors are positioned inside the body of an individual to transfer sensed data to a central station *** great challenges posed to healthcare WBANs are the black hole and sink hole *** from deployed sensor nodes are attracted by sink hole or black hole nodes while grabbing the shortest *** this issue is quite a challenging task as a small variation in medicine intake may result in a severe *** work proposes a hybrid detection framework for attacks by applying a Proportional Coinciding Score(PCS)and an MK-Means algorithm,which is a well-known machine learning technique used to raise attack detection accuracy and decrease computational difficulties while giving treatments for heartache and respiratory ***,the gathered training data feature count is reduced through data pre-processing in the ***,the pre-processed features are sent to the MK-Means algorithm for training the data and promoting ***,certain attack detection measures given by the intrusion detection system,such as the number of data packages trans-received,are identified by the MK-Means *** study demonstrates that the MK-Means framework yields a high detection accuracy with a low packet loss rate,low communication overhead,and reduced end-to-end delay in the network and improves the accuracy of biomedical data.

关键词： Wireless body area network black hole attack sink hole attack proportional coinciding score intrusion detection correlation rate

Value of transient elastography for the prediction of variceal bleeding

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World Journal of Gastroenterology 2011年第17期17卷 2206-2210页

作者： Ioan Sporea Iulia Ratiu roxana Sirli Alina Popescu Simona Bota Department of Gastroenterology University of Medicine and Pharmacy TimiSoara300482 TimiSoaraRomania

AIM:To determine if liver stiffness(LS) measurements by means of transient elastography(TE) correlate with the presence of significant esophageal varices(EV) and if they can predict the occurrence of variceal ***:We studied 1000 cases of liver cirrhosis divided into 2 groups:patients without EV or with grade 1 varices(647 cases) and patients with significant varices(grade 2 and 3 EV)(353 cases).We divided the group of 540 cases with EV into another 2 subgroups:without variceal hemorrhage(375 patients) and patients with a history of variceal bleeding(165 cases).We compared the LS values between the groups using the unpaired t-test and we established cut-off LS values for the presence of significant EV and for the risk of bleeding by using the ROC ***:The mean LS values in the 647 patients without or with grade 1 EV was statistically significantly lower than in the 353 patients with significant EV(26.29 ± 0.60 kPa vs 45.21 ± 1.07 kPa,P < 0.0001).Using the ROC curve we established a cut-off value of 31 kPa for the presence of EV,with 83% sensitivity(95%CI:79.73%-85.93%) and 62% specificity(95% CI:57.15%-66.81%),with 76.2% positive predictive value(PPV)(95% CI:72.72%-79.43%) and 71.3% negative predictive value(NPV)(95% CI:66.37%-76.05%)(AUROC 0.7807,P < 0.0001).The mean LS values in the group with a history of variceal bleeding(165 patients) was statistically significantly higher than in the group with no bleeding history(375 patients):51.92 ± 1.56 kPa vs 35.20 ± 0.91 kPa,P < 0.0001).For a cut-off value of 50.7 kPa,LS had 53.33% sensitivity(95% CI:45.42%-61.13%) and 82.67% specificity(95% CI:78.45%-86.36%),with 82.71% PPV(95% CI:78.5%-86.4%) and 53.66% NPV(95% CI:45.72%-61.47%)(AUROC 0.7300,P < 0.0001) for the prediction of esophageal bleeding.

关键词： Liver stiffness Transient elastography Esophageal varices Variceal bleeding