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Autoantibodies against G-Protein-Coupled Receptors Modulate Heart Mast Cells

Cellular & Molecular Immunology 2007年第2期4卷 127-133页

作者： Ludmila Okruhlicova rosemarie morwinski Wolfgang Schulze Sabine Bartel Peter Weismann Narcisa Tribulova Gerd Wallukat Institute for Heart Research Slovak Academy of Sciences Dubravska cesta 9 PO Box 45 840 05 Bratislava 45 Slovak Republic Max-Delbruck-Center for Molecular Medicine Robert Rssle Str. 10 D-13125 Berlin Germany Max-Delbruck-Center for Molecular Medicine Robert Rssle Str. 10 D-13125 Berlin Germany Medical Faculty Comenius University Institute of Anatomy Sasinkova 2 813 72 Bratislava Slovak Republic Institute for Heart Research Slovak Academy of Sciences Dubravska cesta 9 PO Box 45 840 05 Bratislava 45 Slovak Republic

Mast cells are believed to be involved in myocardial tissue remodelling under pathophysiological conditions. We examined the effects of autoantibodies against G-protein-coupled receptors in sera of patients with heart diseases on myocardial mast cells in the cultured neonatal Sprague-Dawley rat heart cells. Cells collected at day 3 and 10 of the culture were preincubated with autoantibodies against α-adrenoceptor and angiotensin Ⅱ ATl-receptor, agonist phenylephrine and angiotensin Ⅱ, and control IgG. The pretreated cultured cells were stained for selected mast cell markers tryptase, chymase and TNF-α The cultured cells were also processed for observation with electron microscopy. The autoantibodies-treatment of the 3-day cultured cells caused both increased intensity of immunofluorescence （p 〈 0.05） and their enlarged diameters of the mast cells when compared to age-matched ones. In contrast, the fluorescence of preincubated 10-day-old mast cells was decreased compared with controls （p 〈 0.01）. In control samples, the fluorescence of 10-day-old mast cells was significantly higher than that of 3-day-old ones （p 〈 0.001）. Results of electron microscopy examination demonstrated there was an increased granulation of treated 3-day-old mast cells, while a degranulation of mast cells at day 10 of application. The results suggest the modulation effect of the autoantibodies against G-protein-coupled receptors on mast cells, indicating a potential functional link between the autoantibodies against G-protein-coupled receptors and the mast cells in progression of heart disease.

关键词： mast cell autoantibody rat heart cell culture immunofluorescence

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