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Influence of anaerobic fermentation and yeast inoculation on the viability,chemical composition,and quality of coffee

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Food Bioscience 2023年第1期51卷 316-324页

作者： Emerson Josue Martinez Jimenez Pamela Mynsen Machado Martins Ana Luiza de Oliveira Vilela Nadia Nara Batista Sttela Dellyzete Veiga Franco da Rosa Disney Ribeiro Dias rosane freitas schwan Department of Food Science Federal University of LavrasLavrasMGBrazil Department of Agriculture Federal University of LavrasLavrasMGBrazil Department of Biology Federal University of LavrasLavrasMGBrazil Empresa Brasileira de Pesquisa Agropecuaria Embrapa CafeBrasíliaDFBrazil Faculty of Technological Sciences National University of AgricultureThe road to Dulce Nombre de Culmíkm 215neighborhood El EspinoCatacamasHonduras

Microbial metabolites produced during fermentation migrate into the coffee and can influence the decrease in seed viability and coffee *** study evaluated the effects of physiological changes in seed viability on the sensory quality of the beverage using starter cultures through self-induced anaerobic fermentation(SIAF)in Coffea arabica *** natural and pulped *** yeasts were monitored by real-time polymerase chain reaction(qPCR).High-performance liquid chromatography(HPLC)detected citric,malic,and succinic acids in all fermented ***,lactic acid was mainly identified in those coffees processed by the SIAF *** compounds(40)were detected by gas chromatography-mass spectrometry(GC-MS).Alterations in physiological quality were identified,with decreased embryonic viability and cell membrane damage by tetrazolium and electrical conductivity *** fermented coffees obtained scores above 80 *** Torulospora delbrueckii yeast got the best score(86.50)in pulped coffee,and the Candida parapsilosis yeast received the highest score(85.90)in the natural coffee using the SIAF *** coffees were characterized by aromas and flavors of citrus,caramel,honey,chocolate,and *** coffee fermentation process with yeast inoculation affected the coffee bean viability but not the beverage’s sensory quality,indicating that the use of yeast starters by SIAF favors the production of specialty coffees with differentiated sensory characteristics.

关键词： Coffee fermentation Specialty coffees Seed physiological Yeast starter Sensory quality

Effects of yeast fermented soursop and umbu-caja pulps on the colonic microbiota of middle-aged hypertensive adults

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Food Bioscience 2023年第1期51卷 1067-1075页

作者： Elvira de Lourdes Chaves Macedo Tatiana Colombo Pimentel Janne Santos de Morais Dirceu de Sousa Melo Angelica Cristina de Souza Marcos dos Santos Lima Karoliny Brito Sampaio Noadia Priscila Araújo Rodrigues Disney Ribeiro DiasEvandro Leite de Souza rosane freitas schwan Marciane Magnani Laboratory of Microbial Processes in Foods Federal University of Paraiba58051-900Joao PessoaPBBrazil Federal Institute of Parana 87703-536ParanavaíPRBrazil Federal University of Lavras 37200-900LavrasMGBrazil Institute Federal of Sertao Pernambucano 56314-520PetrolinaPEBrazil Laboratory of Food Microbiology Federal University of Paraiba58058-600Joao PessoaPBBrazil

The effects of umbu-caja and soursop pulps fermented by the yeast Issatchenkia terricola 129 on the colonic microbiota of middle-aged hypertensive adults were evaluated in vitro fermentation during 48 *** relative abundance of distinct bacterial groups,pH values,and contents of organic acids,sugars,and phenolic compounds were determined at 0,24,and 48 h of *** boulardii CNCM I-745,a recognized probiotic yeast,was used for comparison *** colonic fermentation,maltose,glucose,and fructose were metabolized,resulting in reduced pH values and decreased relative abundance of Bacteroides spp./Prevotella *** soursop pulp with *** 129 improved the colonic microbiota by increasing the relative abundance of Bifidobacterium *** Lactobacillus *** reducing the relative abundance of Eubacterium rectale/Clostridium coccoides and Clostridium histolyticum,resulting in the highest prebiotic *** the same time,the consumption of rhamnose and gallic acid and higher contents of propionic and acetic acids and procyanidin B2 were *** effects on colonic microbiota were less pronounced for *** followed by umbu-caja pulp with *** *** results indicate that fermented soursop pulp with *** 129 may modulate the colonic microbiota of middle-aged hypertensive adults.

关键词： Colonic fermentation Microbiota Metabolism Spondias spp. Annona muricata L

Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

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Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado rosane L.Lopes Simone S.C.Oliveira Simone P.C.freitas Jacenir R.Santos-Mallet AndréL.S.Santos Denise Feder Suzete A.O.Gomes Programa de Pós-Graduação em Ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Biodiversidade de Insetos e Patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo Cruz Piauí TeresinaPIBrazil Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil Laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease

Physicochemical characterization, the Hirshfeld surface, and biological evaluation of two meloxicam compounding pharmacy samples

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Journal of Pharmaceutical Analysis 2018年第2期8卷 103-108页

作者： Luciana F.A.Romani Maria I.Yoshida Elionai C.L.Gomes Renes R.Machado Felipe F.Rodrigues Marcio M.Coelho Marcelo A.Oliveira Maria B.freitas-Marques rosane A.S.San Gil Wagner N.Mussel Department of Chemistry Institute of Exact Sciences Federal University of Minas Gerais Department of Pharmaceutical Products Faculty of Pharmacy Federal University of Minas Gerais Health Science Department Federal University of Espírito Santo Campus Sao Mateus Institute of Chemistry Federal University of Rio de Janeiro Campus Fundao

Meloxicam(MLX) is an anti-inflammatory drug susceptible to variations and crystalline transitions. In compounding pharmacies, the complete crystallographic evaluation of the raw material is not a routine procedure. We performed a complete crystallographic characterization of aleatory raw MLX samples from compounding pharmacies. X-ray diffraction indicated the presence of two crystalline forms in one sample. DSC experiments suggested that crystallization, or a crystal transition, occurred differently between samples. The FTIR and ~1H NMR spectra showed characteristic assignments.^(13)C solid-state NMR spectroscopy indicated the presence of more than one phase in a sample from pharmacy B. The Hirshfeld surface analysis, with electrostatic potential projection, allowed complete assignment of the UV spectra in ethanol solution. The polymorph I of meloxicam was more active than polymorph III in an experimental model of acute inflammation in mice. Our results highlighted the need for complete crystallographic characterization and the separation of freely used raw materials in compounding pharmacies,as a routine procedure, to ensure the desired dose/effect.

关键词： Meloxicam Polymorphism Hirshfeld surface Anti-inflammatory activity