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Steroidal scaffold decorations in Solanum alkaloid biosynthesis

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molecular Plant 2024年第8期17卷 1236-1254页

作者： rosalind Lucier mohamed O.Kamileen Yoko Nakamura Sofiia Serediuk Ranjit Barbole Jens Wurlitzer maritta Kunert Sarah Heinicke Sarah e.O'Connor Prashant D.Sonawane Department of Natural Product Biosynthesis Max Planck Institute for Chemical Ecology07745 JenaGermany Research Group Biosynthesis and NmR Max Planck Institute for Chemical Ecology07745 JenaGermany Biochemical Sciences Division CSIR-National Chemical LaboratoryPune 411008MaharashtraIndia

Steroidal glycoalkaloids(SGAs)are specialized metabolites produced by hundreds of Solanum species,including important vegetable crops such as tomato,potato,and *** it has been known that SGAs play important roles in defense in plants and“anti-nutritional"effects(e.g.,toxicity and bitterness)to humans,many of these molecules have documented anti-cancer,anti-microbial,antiinflammatory,anti-viral,and anti-pyretic *** these,α-solasonine andα-solamargine isolated from black nightshade(Solanum nigrum)are reported to have potent anti-tumor,anti-proliferative,and anti-inflammatory ***,α-solasonine andα-solamargine,along with the core steroidal aglycone solasodine,are the most widespread SGAs produced among the Solanum ***,it is still unknown how plants synthesize these bioactive steroidal *** comparative metabolomictranscriptome-guided approach,biosynthetic logic,combinatorial expression in Nicotiana benthamiana,and functional recombinant enzyme assays,here we report the discovery of 12 enzymes from *** that converts the starting cholesterol precursorto solasodine aglycone,and the downstreamα-solasonine,α-solamargine,and malonyl-solamargine SGA *** further identified six enzymes from cultivated eggplant that catalyze the production ofα-solasonine,α-solamargine,and malonyl-solamargine SGAs from solasodine aglycone via glycosylation and atypical malonylation *** work provides the gene tool box and platform for engineering the production of high-value,steroidal bioactive molecules inheterologoushosts usingsyntheticbiology.

关键词： steroidal glycoalkaloids Solanum biosynthetic pathway α-solamargine malonyl-solamargine specialized metabolites

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直面实验室欺诈

科学新闻 2024年第2期 57-58页

作者： rosalind A.Coleman 倪伟波

星期一，当C.B.走进我的办公室时，他愁眉苦脸的样子让我知道这是个坏消息。3周前，我的两名博士后出现在我的门口，勉强忍住泪水。他们确信另一个博士后同事伪造了数据，因为他们从未见过约翰（化名）使用其在实验室会议上展示的最新...

星期一，当C.B.走进我的办公室时，他愁眉苦脸的样子让我知道这是个坏消息。3周前，我的两名博士后出现在我的门口，勉强忍住泪水。他们确信另一个博士后同事伪造了数据，因为他们从未见过约翰（化名）使用其在实验室会议上展示的最新数据所必需的设备。起初，我以为他们弄错了，那时我已经在全国性会议上几次展示了约翰的数据，而且当时我们正在写一篇论文，这篇论文将对我们的领域产生巨大影响。但我不能忽视他们的怀疑。我给学校的廉政官员打了电话，见了学院院长，还聘请实验室前资深科学家C.B.重复最关键的实验。

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The role of BRCA 1 and BRCA2 in prostate cancer

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Asian Journal of Andrology 2012年第3期14卷 409-414,I0006页

作者： elena Castro rosalind eeles Oncogenetics Team The Institute of Cancer Research Sutton SM2 5NG UK Academic Urology Unit The Royal Marsden NH Foundation Trust London SW3 6J J UK

One of the strongest risk factors for prostate cancer is a family history of the disease. Germline mutations in the breast cancer predisposition gene 2 （BRCA2） are the genetic events known to date that confer the highest risk of prostate cancer （8.6-fold in men ≤ 65 years）. Although the role of BRCA2 and BRCA1 in prostate tumorigenesis remains unrevealed, deleterious mutations in both genes have been associated with more aggressive disease and poor clinical outcomes. The increasing incidence of prostate cancer worldwide supports the need for new methods to predict outcome and identify patients with potentially lethal forms of the disease. As we present here, BRCA germline mutations, mainly in the BRCA2gene, are one of those predictive factors. We will also discuss the implications of these mutations in the management of prostate cancer and hypothesize on the potential for the development of strategies for sooradic cases with similar characteristics.

关键词： BRCA1 BRCA2 prostate cancer

Tetracycline-inducible protein expression in pancreatic cancer cells: effects of CapG overexpression

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World Journal of Gastroenterology 2011年第15期17卷 1947-1960页

作者： Sarah Tonack Sabina Patel mehdi Jalali Taoufik Nedjadi rosalind e Jenkins Christopher Goldring John Neoptolemos eithne Costello Liverpool CR-UK Centre Department of Molecular and Therapeutic Cancer MedicineUniversity of Liverpool Liverpool L69 3GA United Kingdom mRC Centre forDrug Safety Science Department of Pharmacology and Therapeutics School of Biomedical Sciences University of Liverpool L69 3GA United Kingdom National Institute for Health Research Pancreatic Biomedical Research Unit Liverpool L69 3GA United Kingdom

AIm: To establish stable tetracycline-inducible pancreatic cancer cell lines. meTHODS: Suit-2, miaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-m2), under the control of either a cytomegalovirus (CmV) or a chicken β-actin promoter, and the resulting clones were ***: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2e1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable.

关键词： Pancreatic cancer cells Tetracycline-inducible CapG Suit-2 Panc-1 miaPaCa-2

A living foundry for Synthetic Biological materials:A synthetic biology roadmap to new advanced materials

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Synthetic and Systems Biotechnology 2018年第2期3卷 105-112页

作者： rosalind A.Le Feuvre Nigel S.Scrutton BBSRC/ePSRC manchester Centre for Synthetic Biology of Fine and Speciality Chemicals(SYNBIOCHem) Manchester Institute of BiotechnologyThe University of ManchesterManchester M17DNUnited Kingdom School of Chemistry The University of ManchesterManchester M17DNUnited Kingdom

Society is on the cusp of harnessing recent advances in synthetic biology to discover new bio-based products and routes to their affordable and sustainable *** is no more evident than in the discovery and manufacture of Synthetic Biological materials,where synthetic biology has the capacity to usher in a new materials from Biology era that will revolutionise the discovery and manufacture of innovative synthetic biological *** will encompass novel,smart,functionalised and hybrid materials for diverse applications whose discovery and routes to bio-production will be stimulated by the fusion of new technologies positioned across physical,digital and biological *** article,which developed from an international workshop held in manchester,United Kingdom,in 2017[1],sets out to identify opportunities in the new materials from biology *** considers requirements,early understanding and foresight of the challenges faced in delivering a Discovery to manufacturing Pipeline for synthetic biological materials using synthetic biology *** challenge spans the complete production cycle from intelligent and predictive design,fabrication,evaluation and production of synthetic biological materials to new ways of bringing these products to *** opportunities are identified that will help foster expertise sharing and infrastructure development to accelerate the delivery of a new generation of synthetic biological materials and the leveraging of existing investments in synthetic biology and advanced materials research to achieve this goal.

关键词： Synthetic biology materials Biological materials Biomaterials Advanced materials

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以毕加索之名

湖北美术学院学报 2024年第2期 4-11页

作者：罗莎琳德・克劳斯杨贤宗(译) 张涵青(译) 不详喀什大学美术与设计学院华中师范大学美术学院

罗莎琳德・克劳斯在《以毕加索的名义》一文中,尝试用索绪尔的符号学分析毕加索的拼贴画。她在其中批评了以往将毕加索作品意义理解为对个人事件的反映、风格变化由个人环境变化所决定的看法。克劳斯探讨了专有名称在分析作品含义中的作... 详细信息

罗莎琳德・克劳斯在《以毕加索的名义》一文中,尝试用索绪尔的符号学分析毕加索的拼贴画。她在其中批评了以往将毕加索作品意义理解为对个人事件的反映、风格变化由个人环境变化所决定的看法。克劳斯探讨了专有名称在分析作品含义中的作用,引出符号学中缺场和差异的概念,提出指称对象的不确定性以及艺术史关涉的是内涵而不是外延问题等观点,有助于我们理解符号学方法在美术史研究中的实际应用情况。

关键词：专有名称符号学拼贴画差异

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活性细胞骨架网络中的力传播

医用生物力学 2024年第S1期 401-401页

作者：屈子杰刘世琛 rosalind Wenshan Pan Heun Jin Lee Shahriar Shadkhoo Fan Yang David Larios 李春赫 Rob Phillips matt Thomson 上海交通大学密西根学院美国加州理工学院

在生物系统中,由驱动蛋白和丝蛋白构成的消耗能量的活性网络可以产生驱动力并作用于多种生物过程,包括细胞运动、形状变化和复制等。这种活性网络的功能不可或缺,然而,分子尺度蛋白的相互作用如何能够使力在微米尺度上进行组织和传递仍...

在生物系统中,由驱动蛋白和丝蛋白构成的消耗能量的活性网络可以产生驱动力并作用于多种生物过程,包括细胞运动、形状变化和复制等。这种活性网络的功能不可或缺,然而,分子尺度蛋白的相互作用如何能够使力在微米尺度上进行组织和传递仍然难以捉摸。本研究证明了微管的捆绑(增长)可以使活性物质系统在“全局力传递”相和“局部力耗散”相间相互转化。平均微管长度增加5倍会导致局部相到全局相的转变。转变后,力传播的长度尺度增加100倍并且力的大小增加了20倍。全局相下活性物质系统可产生(10 p N)力场,使包括细胞运输和液滴运动等应用成为可能。通过理论和模拟证明,即使存在少数长微管也可以诱导局部和全局相之间的逾渗转变,为细胞中的力传递提供调节机制。本研究结果揭示了细胞中力传导机制,并使活性材料应用于合成生物学和软机器人成为可能。

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Using a Game to engage Stakeholders in extreme event Attribution Science

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International Journal of Disaster Risk Science 2016年第4期7卷 353-365页

作者： Hannah R.Parker rosalind J.Cornforth Pablo Suarez myles R.Allen emily Boyd Rachel James Richard G.Jones Friederike e.L.Otto Peter Walton Department of meteorology University of Reading Walker Institute University of Reading Red Cross Red Crescent Climate Centre Pardee Center for the Study of the Longer-Range Future Boston University environmental Change Institute University of Oxford Atmospheric Oceanic and Planetary PhysicsUniversity of Oxford Department of Geography and environmental Science University of Reading Present Address:Lund University Centre for Sustainability Studies met Office Hadley Centre School of Geography and the environment University of Oxford

The impacts of weather and climate-related disasters are increasing,and climate change can exacerbate many *** communicating climate risk and integrating science into policy requires scientists and stakeholders to work *** dialogue between scientists and policymakers can be challenging given the inherently multidimensional nature of the issues at stake when managing climate *** on the growing use of serious games to create dialogue between stakeholders,we present a new game for policymakers called Climate Attribution Under Loss and Damage:Risking,Observing,Negotiating(CAULDRON).CAULDRON aims to communicate understanding of the science attributing extreme events to climate change in a memorable and compelling way,and create space for dialogue around policy decisions addressing changing risks and loss and damage from climate *** describe the process of developing CAULDRON,and draw on observations of players and their feedback to demonstrate its potential to facilitate the interpretation of probabilistic climate information and the understanding of its relevance to informing *** looking to engage with stakeholders can learn valuable lessons in adopting similar innovative *** suitability of games depends on the policy context but,if used appropriately,experiential learning can drive coproduced understanding and meaningful dialogue.

关键词： Climate change extreme event attribution Loss and damage policy Participatory games Probabilistic event attribution(PeA) Risk management

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Personal Tacit Knowledge and Global Learning Professional Competencies—multi-Dimensional Relationships

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Journal of Computer and Communications 2017年第13期5卷 21-30页

作者： rosalind R. King University Information Technology Services Kennesaw State University Kennesaw USA

Global learning professional competencies (GLPCs) are essential for college students to be able to address the impact of globalization in the 21st century. Organizations and society-at-large look to higher education to prepare college students with GLPCs. In addition, there is a body of literature that suggest personal tacit knowledge enhance GLPCs. However, researchers have done little from an empirical perspective to determine the relationship between the use of P-T K and enhancement of GLPCs, hence the purpose of this study. The statistical results revealed significant correlations, p st century knowledge society through use of P-T K.

关键词： Personal Tacit Knowledge (P-T K) Global Learning Professional Competencies (GLPCs) multi-Dimensional Relationships