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Cryopreservation by Vitrification of Vitis vinifera cv. ＂Red Globe＂ Zygotic Embryos and Effect on the Expression of DNA Methyltransferase Genes

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Journal of Agricultural Science and Technology(B) 2016年第6期6卷 387-399页

作者： Heriberto Garcia-Coronado Maria-Elena Baez-Flores rosalba troncoso-rojas Marisela Rivera-Dominguez Martin-Emesto Tiznado-Hernandez Coordinacion de Tecnologia de Alimentos de Origen Vegetal Centro de Investigacion en Alimentacion y Desarrollo A.C. (CLAD)Hermosillo Sonora CP 83304 Mexico Facultad de Ciencias Quimico Biol6gicas Universidad Autonoma de Sinaloa Culiacan Sinaloa CP 80013 Mexico Coordinacion de Ciencia de los Alimentos Centro de Investigacion en Alimentacion y Desarrollo A.C. (CLAD) HermosilloSonora CP 83304 Mexico

Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.

关键词： Vitis vinifera zygotic embryo cryopreservation vitrification DNA methyltransferase.

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Role of copper chelating agents: between old applications and new perspectives in neuroscience

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Neural Regeneration Research 2025年第3期20卷 751-762页

作者： rosalba Leuci Leonardo Brunetti Vincenzo Tufarelli Marco Cerini Marco Paparella Nikola Puvača Luca Piemontese Department of Pharmacy-Pharmaceutical Science University of Bari Aldo MoroBariItaly Department of Precision and Regenerative Medicine and Jonian Area(DiMePRe-J) Section of Veterinary Science and Animal ProductionUniversity of Bari Aldo MoroBariItaly Department of Engineering Management in Biotechnology Faculty of Economics and Engineering Management in Novi SadUniversity Business Academy in Novi SadNovi SadSerbia

The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.

关键词： agriculture Alzheimer's disease chelators copper feed supplements multi-target

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News about non-secretory exocytosis: mechanisms, properties, and functions

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Journal of Molecular Cell Biology 2019年第9期11卷 736-746页

作者： rosalba D'Alessandro Jacopo Meldolesi Scientific Institute S.De Beilis Via Turi 277013Castellana GrotteItaly Scientific Institute San Raffaele and Vita Salute San Raffaele University Via Olgettina 5820132MilanItaly

The fusion by exocytosis of many vesicles to the plasma membrane induces the discharge to the extracellular space of their abundant luminal *** exocytic vesicles,however,do not contain cargoes,and thus,their fusion is not followed by ***,two distinct processes of exocytosis exist,one secretory and the other *** present review deals with the knowledge of non-secretory exocytosis developed during recent *** such developments are the dual generation of the exocytic vesicles,initially released either from the trans-Golgi network or by endocytosis;their traffic with activation of receptors,channels,pumps,and transporters;the identification of their tethering and soluble N-ethylmaleimide-sensitive factor attachment protein receptor complexes that govern membrane fusions;the growth of axons and the membrane *** of potential relevance of these processes for pathology and medicine are also *** developments presented here offer interesting chances for future progress in the field.

关键词： exocytosis Golgi complex vesicles endocytosis

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Space-Time Decomposition of Kalman Filter

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Numerical Mathematics(Theory,Methods and Applications) 2023年第4期16卷 847-882页

作者： Luisa D’Amore rosalba Cacciapuoti University of Naples Federico II NaplesItaly

We present an innovative interpretation of Kalman filter(KF)combining the ideas of Schwarz domain decomposition(DD)and parallel in time(PinT)*** we call it *** contrast to standard DD approaches which are already incorporated in KF and other state estimation models,implementing a straightforward data parallelism inside the loop over time,DD-KF ab-initio partitions the whole model,including filter equations and dynamic model along both space and time directions/*** a consequence,we get local KFs reproducing the original filter at smaller dimensions on local ***,sub problems could be solved in *** order to enforce the matching of local solutions on overlapping regions,and then to achieve the same global solution of KF,local KFs are slightly modified by adding a correction term keeping track of contributions of adjacent subdomains to overlapping *** a correction term balances localization errors along overlapping regions,acting as a regularization constraint on local ***,such a localization excludes remote observations from each analyzed location improving the conditioning of the error covariance *** dynamic model we consider shallow water equations which can be regarded a consistent tool to get a proof of concept of the reliability assessment of DD-KF in monitoring and forecasting of weather systems and ocean currents.

关键词： Data assimilation Kalman filter domain decomposition filter localization model reduction numerical algorithm

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Can foods influence the onset and progress of neurodegenerative diseases?

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Neural Regeneration Research 2022年第11期17卷 2443-2444页

作者： Luca Piemontese Leonardo Brunetti rosalba Leuci Department of Pharmacy and Pharmaceutical Sciences University of Bari Aldo Moro

Over the course of the last few years,the advertisement and marketing of commercial food products in the European market has seen an increasing focus on the beneficial effects of such products for consumer health(Regulation,2006).These so-called"health claims", which can be found on the label of some food products,have become a very important medium,aiming to reach the maximum amount of potential consumers possible,and the delivery of these promises has become a crucial objective for food companies or ***,in most cases,the evidence backing up such claims(and their coveted acknowledgment on the product label) has often been cited as insufficient by institutions such as the European Food and Safety Authority (EFSA,Parma,Italy).

关键词： companies Authority claims

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Topographic effects observed at Amatrice hill during the 2016-2017 Central Italy seismic sequence

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Earthquake Engineering and Engineering Vibration 2021年第1期20卷 63-78页

作者： Gerardo Grelle Laura Bonito Maresca rosalba Silvia Iacurto Claudia Madiai Paola Revellino Giuseppe Sappa Department of Civil Building and Environmental EngineeringSapienza University of RomeItaly Department of Sciences and Technologies University of SannioItaly Department of Civil and Environmental Engineering University of FlorenceItaly

The estimate of seismic site effects by experimental approaches is based on different assumptions aimed at simplifying the complex actual site conditions and related ***,the reliability of the results can increase if the experimental data is focused on quite strong seismic sequences and the on-site acquisition of a large number of signals is deemed strategic for the assessment of the expected *** on these considerations,the ground motion at the Red Zone sector of Amatrice hill,violently struck by the 2016-2017 Central Italy seismic sequence,was analyzed via an observational approach.A large set of weak motions(moment magnitude Mw 2.5-3.9)was analyzed in this study by means of standard(SSR)and horizontal to vertical(HVSR)spectral ratio *** results from the experimental analysis of the site effects by using weak motion and noise signals show a significant amplification at the top of Amatrice hill with a remarkable polarization of the motion and changes in spectral shapes according to the topographic setting of the relief.

关键词： topographic effects standard spectra ratio(SSR) horizontal and vertical spectral ratio(HVSR) directional effects

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Adjuvant chemotherapy for gastric cancer:Current evidence and future challenges

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World Journal of Gastroenterology 2014年第16期20卷 4516-4525页

作者： rosalba Miceli Gianluca Tomasello Giacomo Bregni Maria Di Bartolomeo Filippo Pietrantonio Unit of Medical Statistics Biometry and BioinformaticsFondazione IRCCS Istituto Nazionale Tumori20133 MilanItaly Medical Oncology Division Azienda Istituti Ospitalieri di Cremona26100 CremonaItaly Medical Oncology Department Fondazione IRCCS Istituto Nazionale Tumori20133 MilanItaly

Gastric cancer still represents one of the major causes of cancer mortality *** survival is mainly related to stage,with a high proportion of patients with metastatic disease at ***,the cure rate largely depend upon surgical *** the additional,albeit small,benefit of adjuvant chemotherapy has been clearly demonstrated,no general consensus has been reached on the best treatment ***,the narrow therapeutic index of adjuvant chemotherapy(i.e.,limited survival benefit with considerable toxicity)requires a careful assessment of expected risks and benefits for individual *** choices vary widely based on the different geographic areas,with chemotherapy alone more often preferred in Europe or Asia and chemoradiotherapy in the United *** the present review we discuss the current evidence and future challenges regarding adjuvant chemotherapy in curatively resected gastric cancer with particular emphasis on the recently completed landmark studies and *** most recent patient-level meta-analysis demonstrated the benefit of adjuvant chemotherapy over curative surgery;the same Authors also showed that disease free survival may be used as a surrogate end-point for overall *** finally discuss future research issues such as the need of economic evaluations,development of prognostic or predictive biomarkers,and the unmet clinical need of trials comparing perioperative chemotherapy with adjuvant treatment.

关键词： Gastric cancer Adjuvant chemotherapy Radiotherapy Randomized trial

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Electrocatalytic production of glycolic acid via oxalic acid reduction on titania debris supported on a TiO_(2)nanotube array

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Journal of Energy Chemistry 2022年第5期31卷 669-678页

作者： Francesco Pio Abramo Federica De Luca rosalba Passalacqua Gabriele Centi Gianfranco Giorgianni Siglinda Perathoner Salvatore Abate Department of ChiBioFarAM(Industrial Chemistry) University of MessinaERIC aisbl and INSTM/CASPEV.le F.Stagno d’Alcontres 31Messina 98166Italy University of Calabria Department of Environmental EngineeringLaboratory of Industrial Chemistry and Catalysis Via P.Bucci87036 RendeCSItaly

Electrodes prepared by anodic oxidation of Ti foils are robust and not toxic materials for the electrocatalytic reduction of oxalic acid to glycolic acid, allowing the development of a renewable energy-driven process for producing an alcoholic compound from an organic acid at low potential and room temperature. Coupled with the electrochemical synthesis of the oxalic acid from CO_(2),this process represents a new green and low-carbon path to produce added value chemicals from CO_(2). Various electrodes prepared by anodic oxidation of Ti foils were investigated. They were characterized by the presence of a TiO_(2) nanotube array together with the presence of small patches, debris, or TiO_(2) nanoparticles. The concentration of oxygen vacancies, the amount of Ti^(3+) measured by X-ray photoelectron spectroscopy(XPS) and the intensity of the anodic peak measured by cyclic voltammetry, were positively correlated with the achieved oxalic acid conversion and glycolic acid yield. The analysis of the results indicates the presence of small amorphous TiO_(2) nanoparticles(or surface patches or debris) interacting with TiO_(2) nanotubes, the sites responsible for the conversion of oxalic acid and glycolic acid yield. By varying this structural characteristic of the electrodes, it is possible to tune the glycolic acid to glyoxylic acid relative ratio. A best cumulative Faradaic efficiency(FE) of about 84% with FE to glycolic acid around 60% and oxalic conversion about 30% was observed.

关键词： Oxalic acid TiO_(2)nanotubes Glyoxylic acid Glycolic acid Electrocatalysis

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Excess body weight, liver steatosis, and early fibrosis progression due to hepatitis C recurrence after liver transplantation

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World Journal of Gastroenterology 2005年第38期11卷 5944-5950页

作者： Pierluigi Toniutto Carlo Fabris Claudio Avellini rosalba Minisini Davide Bitetto Elisabetta Rossi Carlo Smirne Mario Pirisi Liver Transplantation Unit Pathology Department of Medical Sciences

AIM: To investigate how weight gain after OLT affects the speed of fibrosis progression (SFP) during recurrent hepatitis C virus (HCV) infection of the ***: Ninety consecutive patients (63 males,median age 53 years; 55 with HCV-related liver disease),transplanted at a single institution, were studied. All were followed for at least 2 years after OLT and had at least one follow-up graft biopsy, performed not earlier than 1 year after the transplant operation. For each biopsy, a single,experienced pathologist gave an estimate of both the staging according to Ishak and the degree of hepatic *** SFP was quantified in fibrosis units/month (FU/mo).The lipid metabolism status of patients was summarized by the plasma triglycerides/cholesterol (T/C) ratio. Body mass index (BMI) was measured before OLT, and 1 and 2 years after ***: In the HCV positive group, the highest SFP was observed in the first post-OLT year. At that time point,a SFP ≤0.100 FU/mo was observed more frequently among recipients who had received their graft from a young donor and had a pre-transplant BMI value ＞26.0 kg/m2. At completion of the first post-transplant year, a BMI value ＞26.5 kg/m2 was associated with a T/C ratio ≤1. The proportion of patients with SFP ＞0.100 FU/mo descended in the following order: female recipients with a high T/C ratio, male recipients with high T/C ratio, and recipients of either gender with low T/C ratio. Hepatic steatosis was observed more frequently in recipients who, in the first post-transplant year, had increased their BMI ≥1.5 kg/m2 in comparison to the pre-transplant value. Hepatic steatosis was inversely associated with the staging ***: Among HCV positive recipients, excessweight gain post-OLT does not represent a factor favoring early liver fibrosis development and might even be protective against it.

关键词： Liver transplantation Hepatitis C infection Liver fibrosis Liver steatosis Body mass index

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Semiconductor,molecular and hybrid systems for photoelectrochemical solar fuel production

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Journal of Energy Chemistry 2017年第2期26卷 219-240页

作者： rosalba Passalacqua Siglinda Perathoner Gabriele Centi Department of Chemical BiologicalPharmaceutical and Environmental SciencesUniversity of MessinaV.le F.Stagno d’Alcontres 3198166 MessinaItaly Department of Mathematics and Computational Sciences Physical Sciences and Earth SciencesUniversity of MessinaV.le F.Stagno d’Alcontres 3198166 MessinaItaly CASPE(INSTM Laboratory of Catalysis for Sustainable Production&Energy)/ERIC aisbl(European Research Institute of Catalysis) University of MessinaV.le F.Stagno d’Alcontres 3198166 MessinaItaly

The paper shortly reviews the basic direct approaches applied in searching for viable solutions to solar fuel production. These are generally distinguished in molecular and semiconductor(non-molecular)systems, however, hybrid strategies, proposed recently, have also been included. The most promising efforts are considered, highlighting key aspects and emerging critical issues. Special attention is paid to aspects such as electrode architecture, device design, and main differences in the scientific vision and challenges to directly produce solar fuels. This overview could be useful to orientate the readers in the wide panorama of research activities concerning water splitting, natural and artificial photosynthesis, and solar fuel production through the identification of common aspects, specialties and potentialities of the many initiatives and approaches that are developing worldwide in this field with the final aim to meet world energy demand.

关键词： Solar fuels production CO2 reduction Artificial photosynthesis PEC cells Semiconductor systems Molecular systems Hybrid nanobiocatalytic systems

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