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Advances in Microbiology 2019年第10期9卷 893-903页

作者： Maria Eduarda de Farias Albuquerque Gaspar de Oliveira Christina Alves Peixoto rosa valéria da silva amorim Programa de Pós-Graduação em Morfotecnologia Departamento de Histologia e Embriologia Universidade Federal da Pernambuco (UFPE) Recife Brazil Departamento de Ultraestrutura Instituto Aggeu Magalhães/Fundação Oswaldo Cruz Recife Brazil

The continuous search for an antimicrobial agent led to the identification of potential antimicrobial biomaterials based on polymers naturals, such as chitosan (CS). However, the mechanism of action of antibacterial activity of CS for gram-positive and gram-negative bacteria was not completely elucidated. The aim of this work is to report the antibacterial activity of CS through ultrastructural analyses of the clinical isolates Staphylococcus aureus and Escherichia coli by Transmission Electron Microscopy. The CS has a bactericidal action against S. aureus and E. coli which alters its cellular ultrastructure, such as with collapsed cell walls, condensed chromatin and the increase of intracellulares structures like vacuoles and cell debris. In this way, the CS represents a potential model for the future design of antibacterial in order to control bacterial resistance of patients in hospital settings.

关键词： Antibacterial Activity Chitosan Ultrastructure Staphylococcus aureu Escherichia coli

Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures

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Journal of Animal Science and Biotechnology 2018年第1期9卷 208-216页

作者： Aline Gomes da silva Mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da silva amorim Román Enrique Maza Ortega Victor valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt Universidade Federal de Vicosa VicosaMinas Gerais 36570-000Brazil Universidade Federal de Mato Grosso do Sul Campo GrandeMato Grosso do Sul 79074-460Brazil Universidade Estadual de Mato Grosso do Sul AquidauanaMato Grosso do Sul 79200-000Brazil Biotran Biotecnologia e Treinamento em Reproducao Animal AlfenasMinas Gerais 37130-000Brazil Universidade Federal do Acre Rio BrancoAcre 69920-900Brazil

Background： Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments： control, which received no supplement; supplementation for the last 30 d of gestation（30-d; 3.0 kg/d）; supplementation for the last 60 d of gestation（60-d; 1.5 kg/d）; or supplementation for the last 90 d of gestation（90-d; 1.0 kg/d）. All supplemented treatments received the same total amount of supplement throughout the experiment： 90 kg（20% of crude protein）. A second experiment（Experiment 2） was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d ***： There was a linear effect of the number of days of supplementation on calving body weight（BW; P 〈 0.05）and a quadratic effect on BW change from parturition to d 31 post-calving（P 〈 0.05）, with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW（P 〉 0.10）. A significant linear effect on interval from parturition to conception（P 〈 0.05） was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility（P 〉 0.10）. Nitrogen excreted through urine tended to increase linearly with the level of supplementation（P 〈 0.10）.Conclusion： Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of

关键词： Flushing Nutrition Parturition Reproduction

Litter removal impacts on soil biodiversity and eucalypt plantation development in the seasonal tropics

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Journal of Forestry Research 2023年第3期34卷 735-748页

作者： Jonas Inkotte Barbara Bomfim Sarah Camelo da silva Marco Bruno Xavier valadão Márcio Gonçalves da rosa Roberta Batista Viana Alcides Gatto Reginaldo SPereira Department of Forestry Engineering University of BrasiliaBrasíliaDistrito FederalBrazil Climate and Ecosystem Sciences Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA SESI/SENAI Social Service Industry Santa Catarina StateChapecóBrazil

The little layer of tree plantations provides primary nutrients for uptake,buff ers changes in soil moisture,and provides habitat and substrate to soil epigeic ***,this layer in eucalypt plantations is often removed to reduce fuel load during the fi re season in the Brazilian savanna(Cerrado).Therefore,it is necessary to quantify the eff ects of changes in litter dynamics on the function of these plantations,on key nutrient cycling processes and on epigeic fauna diversity and *** two adjacent stands(one juvenile and one mature),the consequences of two years of litter removal were quantifi ed as monthly litterfall,leaf and fi ne wood litter decomposition,epigeic fauna abundance and diversity,soil biogeochemical variables,and tree diameter and basal area *** litterfall rates in juvenile and mature stands did not change with litter removal over the study *** litterfall ranged from 4.1 to 4.9 Mg ha^(−1)a^(−1)in litter removal plots and from 3.9 to 4.8 Mg ha^(−1)a^(−1)in control *** wood litter decomposition was slower in litter removal plots compared to controls,while leaf decomposition rates were similar in *** years of litter removal in the juvenile stand did not aff ect topsoil biogeochemical parameters but decreased available phosphorus at 20–40 cm depth relative to *** the mature stand,total cation exchange capacity(0–20 cm)was higher in controls(6.4 cmol c dm^(−3))relative to litter removal plots(6.3 cmol c dm^(−3)),while soil moisture(0–40 cm depth)was lower in litter removal(25.45 m 3 m^(−3))compared to control plots(26 m^(3)m^(−3))in the dry season.A non-metric multidimensional scaling ordination revealed an increased homogeneity in epigeic fauna where litter was ***,decomposition,diameter increment,four soil physical parameters and fourteen chemical parameters at 0–20 and 20–40 cm depth explained the diff erences in soil epigeic fauna composition between litter removal and control p

关键词： Cerrado Nutrient cycling Soil ecology Soil epigeic fauna Plantation management

Flow Dynamics around Two Side by Side Circular Cylinders with Alternating Movements

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World Journal of Mechanics 2023年第1期13卷 1-19页

作者： Alice rosa da silva Faculty of Civil Engineering Federal University of Uberlandia Uberlandia-MG Brazil

The flow dynamics is analyzed through two-dimensional numerical simulations around two circular cylinders arranged side by side, with 4 combinations of alternating motions. All simulations are performed for Re = 1000, amplitude of oscillation (A) equal to 3, frequency ratio (fr) of 0.5, specific rotation (α) equal to 0.5 and different values of spacing ratio (L/D). It is verified that the combination of the type of movement, together with the position of one cylinder in relation to the other, exerts significant influence on the flow dynamics, as well as on the pressure distribution around the cylinder surface and on the average values of the fluid dynamics coefficients. The smallest value of the average pressure coefficient (Cp = -3.3), is obtained for the oscillating cylinder when placed side by side with the clockwise rotation cylinder, case 3 and L/D = 1.5. On the other hand, the lowest mean drag coefficient (Cd = 1.0788), is obtained for the cylinder with counterclockwise rotation, located in the lower position in relation to oscillating cylinder in the upper position, with spacing between them of 1.5. Furthermore, it is observed that the rotation movement is more effective in reducing drag than the rotation-oscillation movement, for the studied frequency ratio.

关键词： Rotation Rotation-Oscillation Flow Dynamic Circular Cylinder

Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacte#um tumefaciens

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Molecular Plant 2017年第2期10卷 353-356页

作者： Tábata rosas-Díaz Pepe Cana-Quijada Vitor amorim-silva Miguel A. Botella rosa Lozano-Durán Eduardo R. Bejarano Shanghai Center for Plant Stress Biology (PSC) Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 201602 China

Dear Editor Research in molecular plant biology has largely benefitted from technologies allowing exogenous gene expression. While the generation of stable transgenic lines is required in some cases, transient transfo... 详细信息

关键词： Suitable Transient tumefaciens

Co-expression of HBZ,TAX and FOXp3 and HTLV-1-associated myelopathy/tropical spastic paraparesis development in HTLV-1-infected individuals:A systematic review

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Asian Pacific Journal of Tropical Medicine 2021年第8期14卷 333-340页

作者： Ana Carolina Marinho Monteiro Lima Greice Carolina Santos da silva Fernanda Khouri Barreto Filipe Ferreira de Almeida Rego Luana Leandro Gois Luciane amorim Santos Catholic University of Salvador SalvadorBahiaBrazil Multidisciplinary Institute in Health Federal University of BahiaVitoria da ConquistaBahiaBrazil Bahia School of Medicine and Public Health SalvadorBahiaBrazil Postgraduate Program in Health Sciences Faculty of Medicine of BahiaFederal University of BahiaSalvadorBahiaBrazil

Human T-cell lymphotropic virus type 1(HTLV-1)is associated with the development of HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP).It has been reported that the HTLV-1 proteins(specifically TAX and HBZ)can modulate FOXp3,resulting in an immune imbalance that can favor the progression of HAM/*** review aims to summarize the literature in order to clarify the relationship between the expression of HTLV-1 m RNAs and/or viral proteins(TAX and HBZ)with the expression of mRNA and/or protein FOXp3 and their correlation with HAM/TSP *** systematic review was conducted according to the recommendations of the Preferred Reporting Items for Systematic Reviews and *** search strategy was performed on the Medical Literature Analysis and Retrieval System Online and Latin American and Caribbean Literature in Health Sciences Platform using subject *** screening,six articles were included in this *** studies suggested that TAX and HBZ have a directly proportional correlation with FOXp3 in individuals with HAM/TSP,which also presented an increased expression of FOXp3 compared to asymptomatic controls and/or healthy *** systematic review indicates that TAX and HBZ can interact with FOXp3 and that interaction may influence HAM/TSP development.

关键词： HTLV-1 HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP) FOXp3 HBZ TAX

A Model Structured in Classes for the Study of Student Population Evolution

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上海大学学报（自然科学版） 2014年第2期20卷 127-137页

作者： PEREIRA Edgar rosa Cecilia silva Jacques Department of Mathematics Universidade Federal do Rio Grande do Norte Department of Mathematics Instituto Politecnico da Guarda Department of Mathematics Universidade Federal do Rio Grande do Sul

This paper presents an application of a metapopulation model structured in classes for the study of population evolution of a network of *** global dynamics are considered in two parts,the local,where the evolution for each grade in a cycle is accounted,and the metapopulation or the movement of students between *** of the model is presented.

关键词：应用数学数学分析数学理论数学概念

Pulmonary Hyalinizing Granuloma: A Case Report

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Open Journal of Thoracic Surgery 2016年第4期6卷 70-73页

作者： Elias amorim Raimunda Ribeiro da silva Armando da Veiga Cruz Filho Presidente Dutra Hospital of the Federal University of Maranhã o S&atilde o Luiz Brazil University Hospital of the Federal University of Maranhã o S&atilde o Luiz Brazil

Pulmonary hyalinizing granuloma (PHG) is a rare nodular disease of unknown etiology. PHG can present with solitary or bilateral lung nodules and be asymptomatic or present with fever, cough, dyspnea, chest pain and he... 详细信息

关键词： Hyalinizing Granuloma Pulmonary Nodular

Systematic Review Study on the Impact of the Pandemic on Literacy:Experiences of Parents and Teachers

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Psychology Research 2022年第10期12卷 813-823页

作者： Liliane da Veiga silva amorim Simone Aparecida Capellini Giseli Donadon Germano São Paulo State University“Júlio de Mesquita Filho”(UNESP) MaríliaBrazil

With the COVID-19 pandemic,it was necessary to carry out public health measures,including the adoption of remote teaching and modification of the educational practices and experiences of parents and ***,the objective of this study was to conduct a systematic review and analyze the impact of the pandemic on the learning of schoolchildren in the early years of literacy,according to the experiences and practices of parents and *** systematic review is based on the PRISMA method(Preferred Reporting Items for Systematic Reviews and Meta-Analyses)and the guiding questions of the PICO strategy(Population,Intervention,Comparison,Outcome).The PubMed,LILACS,Scopus,SciELO,Embase,and Web of Science databases were *** selection criteria involved the descriptors elementary school AND COVID-19,elementary school AND pandemic,and elementary school AND SARS-CoV-2.A total of 130 studies were selected,and after applying the criteria,only six studies were *** studies used interviews and questionnaires aimed at parents,teachers,and students and were carried out in various countries:one in Saudi Arabia,two in Indonesia,one in the United States of America,one in Canada,and one in *** studies were classified as Evidence Level 4(clinical outcome studies).As common points,most parents were stressed,overloaded,and unprepared to support their children during remote *** also mentioned difficulties,such as the lack of support and training,which affected their *** studies should be carried out to verify the educational and social impacts resulting from remote teaching.

关键词： pandemic elementary school COVID-19 online learning

Rainwater Harvesting in Multifamily Social Interest Housing

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Journal of Civil Engineering and Architecture 2015年第10期9卷 1261-1268页

作者： Regina Lucia Melo de Oliveira Marilia Karla da silva Santos Simone rosa da silva Polytechnic School of the University of Pernambuco Recife 50720-001 Brazil

This study evaluated the reduction of water consumption in a SIH （social interest housing） complex from the simulation of implanting a rainwater harvesting system. The methodology consisted of characterization of the case study, survey data of water consumption and data of precipitation in the area, on-site visits to define the average consumption and monthly water distribution, and sizing of a rainwater harvesting tank following the Netuno Program. It was obtained 22.500 L as the ideal volume for the tank, supplying the demand for drinkingwater in 32%, although the reduction in the drinking water bills would be minor, since the object of the case study is considered SIH, and residents pay a fixed fee for consuming up to 10 m3 per month. Therefore, it becomes necessary to analyze this situation from a sustainable and environmental perspective, and the benefits are no longer only economic, but rather they take on a more comprehensive social dimension.

关键词： Rational use of water rainwater harvesting rainwater collection social interest housing.