检索结果-南通市图书馆

Corneal endothelial cells and acoustic cavitation in phacoemulsification

World Journal of Clinical Cases 2023年第8期11卷 1712-1718页

作者： Kai Chen Wen-ya Xu Si-Si Sun Hong-Wei Zhou Department of Ophthalmology Lianshui County People's HospitalHuai’an 223400Jiangsu ProvinceChina Department of Ophthalmology Huai’an 82 HospitalHuai'an 223001Jiangsu ProvinceChina Department of Industrial Engineering Tsinghua UniversityBeijing 100084China

Postoperative complications of phacoemulsification,such as corneal edema caused by human corneal endothelial cell(CEC)injury,are still a matter of *** several factors are known to cause CEC damage,the influence of ultrasound on the formation of free radicals during surgery should be *** in aqueous humor induces cavitation and promotes the formation of hydroxyl radicals or reactive oxygen species(ros).ros-induced apoptosis and autophagy in phacoemulsification have been suggested to significantly promote CEC *** cannot regenerate after injury,and measures must be taken to prevent the loss of CEC after phacoemulsification or other CEC *** can reduce the oxidative stress injury of CEC during *** from rabbit eye studies shows that ascorbic acid infusion during operation or local application of ascorbic acid during phacoemulsification has a protective effect by scavenging free radicals or reducing oxidative *** in experiments and clinical practice,hydrogen dissolved in the irrigating solution can also prevent CEC damage during phacoemulsification ***(AST)can inhibit oxidative damage,thereby protecting different cells from most pathological conditions,such as myocardial cells,luteinized granulosa cells of the ovary,umbilical vascular endothelial cells,and human retina pigment epithelium cell line(ARPE-19).However,existing research has not focused on the application of AST to prevent oxidative stress during phacoemulsification,and the related mechanisms need to be *** Rho related helical coil kinase inhibitor Y-27632 can inhibit CEC apoptosis after *** experiments are required to confirm whether its effect is realized through improving the ros clearance ability of CEC.

关键词： Cataract Phacoemulsification Corneal endothelial cells Ultrasound Acoustic cavitation Oxidative stress Antioxidant

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lipidomic Analysis Reveals the Importance of GIPCs in Arabidopsis Leaf Extracellular Vesicles

引用

Molecular Plant 2020年第10期13卷 1523-1532页

作者： Ning-Jing Liu Ning Wang Jing-Jing Bao Hui-Xian Zhu Ling-Jian Wang Xiao-ya Chen State Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences/Shanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesFenglin Road 300Shanghai 200032China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475004China University of Chinese Academy of Sciences Shanghai 200032China

Plant extracellular vesicles(EVs)are membrane-enclosed nanoparticles that play diverse roles in plant development and ***,impressive progress has been made in the isolation and identification of the proteins and RNAs carried in plant EVs;however,the analysis of EV lipid compositions remains ***,we performed lipidomic analysis of Arabidopsis rosette leaf EVs,revealing a high abundance of certain groups of lipids,in particular sphingolipids,in the ***,the EV sphingolipids are composed of nearly pure glycosylinositolphosphoceramides(GIPCs),which are green lineage abundant and negatively *** further showed that the Arabidopsis TETRASPANIN 8(TET8)knockout mutant has a lower amount of cellular GIPCs and secrets fewer EVs,companied with impaired reactive oxygen species(ros)burst toward *** application of GIPCs promoted the secretion of EVs and ros burst in both the WT and tet8 *** characteristic enrichment of sphingolipid GIPCs provides valuable insights into the biogenesis and function of plant EVs.

关键词： sphingolipids extracellular vesicles

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Chinese propolis on PC-PLC activity and TLR4 expression in LPS-treated vascular endothelial cells

引用

中国病理生理杂志 2011年第7期27卷 1257-1263页

作者： XUAN Hong - zhun SANG Qing LI ya - jing HU Fu - liang College of Animal Sciences Zhejiang University Hangzhou 310029 China School of Life Science. Liaocheng UniversityLiaocheng 252059 China Zhejiang Economic and Trade Polytechnic Hangzhou 310018 China

AIM:To investigate the effect of Chinese propolis on the activity of phosphatidylcholine-specific phospholipase C (PC-PLC)and the expression of Toll-like receptor 4 (TLR4)in LPS-treated vascular endothelial cells (VECs).METHODS:Confluent VECs were stimulated with LPS at the concentration of 100 μg/L in the presence of 0.5% fetal bovine *** cells were treated with Chinese propolis at the concentration of 12.5 mg/L for 12 h and 24 *** viability of VECs 'and the level of nitric oxide (NO)were detected by sulforhodamine B (SRB)assay and chemical method,*** activity of PC-PLC was measured using L-a-phosphatidylcholine as *** protein levels of TLR4,nuclear factor-KB p65 (NF-KB p65)and p53 were determined by Western *** level of intracellular reactive oxygen species (ros)was examined using a fluorescent probe,2',7'-dichlorodihydrofluorescin (DCHF).For the measurement of mitochondrial membrane potential,the fluorescent dye JC-1 was ***:Treatment with Chinese propolis for 24 h had no effect on the viability of ***,the levels of NO and ros were significantly decreased by Chinese ***-PLC activity and NF-KB p65 expression were significantly depressed by Chinese propolis treated for 12 h,and the expression of TLR4 and p53 were dramatically decreased by Chinese propolis treated for 12 and 24 *** effect of Chinese propolis on mitochondrial membrane potential was ***:Chinese propolis depresses the activity of PC-PLC and the expression of TLR4,and then inhibits the downstream signal molecules such as NF-KB p65,p53,ros and NO in VECs.

关键词：病理学医学临床生理

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

All-trans retinoic acid increases ARPE-19 cell apoptosis via activation of reactive oxygen species and endoplasmic reticulum stress pathways

引用

International Journal of Ophthalmology(English edition) 2020年第9期13卷 1345-1350页

作者： Juan Wu Zhen-ya Gao Dong-Mei Cui Hong-Hui Li Jun-Wen Zeng State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Xuchang University School of MedicineXuchang 461000Henan ProvinceChina Chengdu Aier Eye Hospital Chengdu 610000Sichuan ProvinceChina

AIM:To explore the apoptosis of ARPE-19 cells after the treatment with different doses of all-trans-retinoic acid(ATRA).METHODS:ARPE-19 cells were used in the in-vitro *** cytometry assay was employed to evaluate the level of reactive oxygen species(ros)and *** effects of ATRA(concentrations from 2.5 to 20μmol/L)on the expression of endoplasmic reticulum stress(ERS)markers in vitro were evaluated by Western blot and realtime quantitative polymerase chain reaction(qRT-PCR)*** contribution of ros and ERS-induced apoptosis in vitro was determined by using N-acetyl-L-cysteine(NAC)and Salubrinal,an antagonist of NAC and ERS,***:Flow cytometry showed that ATRA significantly increased ARPE-19 cell apoptosis and ros levels in each group(F=86.39,Pros and ERS signaling pathways.

关键词： all-trans-retinoic acid retinal pigment epithelium apoptosis reactive oxygen species endoplasmic reticulum stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

从肝治心组方通过Nrf2/HO-1通路对缺氧/复氧损伤H9c2心肌细胞铁死亡的影响

引用

中药药理与临床 2024年第1期40卷 16-23页

作者：何飘朴美虹谢丽华曾阳王瑾茜汪辛强张程程胡国恒陈亚湖南中医药大学第一附属医院长沙410007 湖南中医药大学长沙410208

目的:基于核因子E2相关因子2(Nrf2)/血红素氧合酶1(HO-1)信号通路探讨从肝治心组方对大鼠H9c2心肌细胞铁死亡的影响。方法:本研究采用H9c2心肌细胞缺氧/复氧(H/R)方法模拟心肌缺血再灌注损伤(MIRI)细胞模型,细胞分为:空白对照组、模型... 详细信息

目的:基于核因子E2相关因子2(Nrf2)/血红素氧合酶1(HO-1)信号通路探讨从肝治心组方对大鼠H9c2心肌细胞铁死亡的影响。方法:本研究采用H9c2心肌细胞缺氧/复氧(H/R)方法模拟心肌缺血再灌注损伤(MIRI)细胞模型,细胞分为:空白对照组、模型对照组、15%正常对照血清组、26.6 g/kg从肝治心组方含药血清15%组、NK25220μmol/L组、ML3855μmol/L组、26.6 g/kg从肝治心组方含药血清15%+ML3855μmol/L组,通过CCK-8法检测细胞活力;DCFH-DA荧光探针检测细胞内活性氧(ros)水平;TMRE荧光探针检测细胞线粒体膜电位水平;FerroOrange荧光探针检测细胞内Fe^(2+)水平;RT-qPCR、Werstern blot法检测核因子E2相关因子2(Nrf2)、血红素氧合酶1(Ho1)、膜铁转运辅助蛋白(Heph)、二价金属离子转运体(Dmt1)、酰基辅酶A合成酶长链家族成员4(Acsl4)mRNA和蛋白表达。结果:与正常对照组比较,模型对照组线粒体膜电位水平降低,ros及Fe^(2+)水平升高,Nrf2、Ho1、Heph mRNA及蛋白表达下调,Dmt1、Acsl4 mRNA及蛋白表达上调(Pros及Fe^(2+)水平降低,Nrf2、Ho1、Heph mRNA及蛋白表达上调,Dmt1、Acsl4 mRNA及蛋白表达下调(Pros及Fe^(2+)水平降低,Nrf2、Ho1、Heph mRNA及蛋白表达上调,Dmt1、Acsl4 mRNA及蛋白表达下调(P<0.05或P<0.01)。结论:从肝治心组方可能通过调控Nrf2/HO-1信号通路,减轻缺氧/复氧损伤的H9c2心肌细胞铁死亡。

关键词：从肝治心组方铁死亡心肌缺血再灌注损伤缺氧/复氧核因子E2相关因子2/血红素氧合酶1

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

多酚治疗心血管疾病研究的文献计量学可视化分析

引用

医学信息 2024年第4期37卷 27-33页

作者：黄筱钧雷亚琪美杨锦代佳莹湖北民族大学医学部

目的对多酚及多酚类化合物治疗心血管疾病相关文献进行文献计量学可视化分析，揭示该领域近11年的研究方向和现状，探索研究热点及前沿方向，分析核心作者及机构，为科研实验及临床诊疗提供新的线索。方法运用文献计量学方法，以Web o... 详细信息

目的对多酚及多酚类化合物治疗心血管疾病相关文献进行文献计量学可视化分析，揭示该领域近11年的研究方向和现状，探索研究热点及前沿方向，分析核心作者及机构，为科研实验及临床诊疗提供新的线索。方法运用文献计量学方法，以Web of Science Core Collection数据库为数据来源，借助CiteSpace软件，采用分类、聚类、突现等方法对2011-2021年多酚治疗心血管疾病的相关研究的发文时间、研究方向分布、作者、国家和机构合作、关键词等进行可视化分析。结果共纳入3604篇文献，发文量最高的国家是中国（488篇）；研究方向主要集中在营养与膳食、食品科学技术；发文量最高的机构是巴塞罗那大学（73篇）；引文量最高的作者是Del rio D；该领域主要研究热点是氧化应激导致慢性疾病的发生机制、多酚和黄酮在慢性疾病中的重要作用；研究前沿是以肠道菌群为靶点探索多酚治疗心血管疾病的新型治疗模式。结论近10年来，氧化应激导致慢性疾病的发病机制、多酚及多酚类化合物和黄酮及黄酮类化合物通过抗氧化作用抑制炎症反应，降低心血管疾病的发生风险是多酚治疗心血管疾病领域的研究热点。多酚治疗心血管疾病在近年内得到一定发展，尤其在以肠道菌群为靶点的治疗方面研究广泛，但其具体机制尚未厘清，需要长期、稳定的研究团队进一步挖掘。

关键词：多酚心血管病文献计量学可视化分析

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Yiqi-Liangxue Recipe Improves Recovery of Injured Endothelia by Promoting the Proliferation and Migration of Vascular Endothelial Cells and Balancing Damage-associated in Flammatory Mediators

引用

World Journal of Traditional Chinese Medicine 2016年第4期2卷 55-61页

作者： Fei Su Xiao-Yun Cui ya-Nan Sun Qian Lin China-Japan friendship Hospital Dong Fang Hospital Beijing University of Chinese Medicine Medical Experimental Center China Academy of Traditional Chinese Medicine

Aim: Yiqi-Liangxue Recipe(YL) is a compound preparation of Chinese medicine used for preventing cardiovascular events after percutaneous coronary intervention(PCI)(Patent No. 200810240175.4). Maintaining the integrity of endothelia is one of the most effective approaches to prevent restenosis. Given its clinical protective effects on long-term prognosis, we investigated the mechanisms of YL in protecting vascular endothelial ***: We prepared drugs serum and human umbilical vein endothelial cell(HUVEC) lines. The injured model was employed with Angiotensin II(Ang II). The YL group was employed with YL treatment. The ARB medicine group was employed with Losartan Potassium treatment. The combination of Chinese medicine and western medicine(YL+ARB) group was employed with both YL and ARB. The control group was unemployed with injury and medical treatment. For each group the cell migration rate(CM) and cell proliferation rate(CP) were measured. The concentration of Nitric Oxide(NO), Reactive Oxygen Species(ros), Endothelin-1(ET-1) were assessed. The gene expression level of ET-1 was ***: YL+ARB group significantly promoted the speed of the CM/CP of the injured HUVECs. Compared with model group, the concentration of NO increased after the drug intervention, and the concentration of ET-1 decreased. Compared with YL+ARB group, YL and ARB group each had the similar weaker effects, but did not have significant difference. The fluorescence of ros in YL, ARB and YL+ARB group had no difference because the fluorescence was too strong. Compared with model group, the-2ΔΔCt values were decreased in the YL, ARB and YL+ARB group. The gene expression level of ET-1 was inhibited after the drug intervention, although with no significant ***: Treatment with YL ameliorates the injury of vascular endothelial cells and YL+ARB has better curative effect. The mechanisms are associated with improving the speed of the CM/CP; increasing the release of NO a

关键词： Yiqi-Liangxue Recipe PCI Vascular endothelial cells Cell migration Damage-associated mediators

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Putrescine, spermidine, and spermine play distinct roles in rice salt tolerance

引用

Journal of Integrative Agriculture 2020年第3期19卷 643-655页

作者： Md Azizul ISlam PANG Jin-huan MENG Fan-wei LI ya-wen XU Ning yaNG Chao LIU Jun State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101P.R.China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049P.R.China Department of Biotechnology and Genetic Engineering Faculty of Applied Science and TechnologyIslamic UniversityKushtia 7003Bangladesh

Polyamines(PAs) play diverse roles in plant growth and development,as well as responses to environmental *** this study,the effects of PAs on rice salt tolerance were *** stress resulted in the alteration of endogenous PAs levels in rice roots and leaves,where spermidine(Spd) and spermine(Spm) contents were increased,and putrescine(Put) content was ***-qPCR analysis revealed that PAs biosynthesis-related genes ADC1,ODC,and Arginase were significantly downregulated by salt treatment;however,SamDC transcription was significantly *** Spm enhanced rice salt tolerance remarkably;however,exogenous Put and Spd undermined rice salt *** rice plants overexpressing SamDC display a higher ratio of Spm/(Put+Spd) and enhanced salt *** stress also increased polyamine oxidase activities in rice,resulting in elevated reactive oxygen species(ros) *** findings revealed that accumulation of Put and Spd substantially reduced salt tolerance in rice,likely by facilitating ros production;whereas,conversion of Put and Spd to Spm contributes to rice salt tolerance.

关键词： rice polyamines NaCl polyamine oxidase guazatine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

当归多糖促进骨髓移植重建受体小鼠造血功能

引用

解剖学报 2024年第5期55卷 556-564页

作者：杨婷廖奎黄彩虹魏晗王程杜坤航汪子铃王璐王亚平重庆医科大学组织学与胚胎学教研室干细胞与组织工程研究室重庆医科大学第一附属医院肿瘤科重庆400016

目的探讨当归多糖(ASP)调控骨髓基质细胞(BMSCs)促进供体骨髓移植(BMT)重建受体小鼠造血功能的机制。方法分离纯化8~10周龄雄性C57BL/6J小鼠骨髓单个核细胞(BMMNCs),移植给同龄同种雌性受体小鼠,第9天取受体鼠BMMNCs,再次移植给雌性受... 详细信息

目的探讨当归多糖(ASP)调控骨髓基质细胞(BMSCs)促进供体骨髓移植(BMT)重建受体小鼠造血功能的机制。方法分离纯化8~10周龄雄性C57BL/6J小鼠骨髓单个核细胞(BMMNCs),移植给同龄同种雌性受体小鼠,第9天取受体鼠BMMNCs,再次移植给雌性受体小鼠。移植受体鼠分为对照组:假照射;辐射组:8.0 Gy X射线全身一次性照射;骨髓移植组:照射同辐射组,经尾静脉移植雄性供体BMMNCs(5×10^(6)个细胞);骨髓移植ASP组:同骨髓移植组,从移植的第1天起连续腹腔注射ASP[100 mg/(kg·d)×9]。模型构建期间记录小鼠体重与生存变化,模型构建结束后采集受体小鼠BMMNCs检测Y染色体性别决定区(SRY)基因,测定外周血血常规指标,计算股骨BMMNCs数,观察骨髓组织病理学;分离培养受体鼠BMSCs,观察细胞贴壁生长,5-乙炔基-2’-脱氧尿苷(EdU)法检测BMSCs增殖;分析细胞内活性氧(ros)水平、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;检测BMSCs培养上清液中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、干细胞因子(SCF)和胰岛素样生长因子1(IGF-1)水平,检测BMMNCs与各组BMSCs共培养48 h,形成造血祖细胞混合集落(CFU-Mix)数量;Real-time PCR分析受体BMMNCs的Notch信号通路相关基因(Notch1、Jagged1、Hes1)表达。结果单纯辐射组小鼠全部死亡,骨髓移植ASP组受体鼠体重下降不明显;在受体雌鼠BMMNCs中检测到SRY基因;骨髓移植ASP组受体鼠外周血血常规和BMMNCs总数下降幅度不显著,骨髓组织病理学损伤减轻;ASP能促进BMSCs增殖,降低BMSCs中ros、MDA含量,提高SOD活性;促进BMSCs分泌SCF、GM-CSF及IGF-1,提高BMMNCs与受体BMSCs共培养后的CFU-Mix产率;提高受体小鼠BMMNCs中Notch1、Jagged1、Hes1 mRNA表达。结论ASP促进受体小鼠造血功能重建机制与减轻造血微环境氧化应激损伤,提高BMSCs分泌造血生长因子,调控Notch信号通路有关。

关键词：当归多糖 X射线辐射骨髓移植造血重建骨髓微环境免疫荧光实时定量聚合酶链反应小鼠

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vacuolar processing enzyme positively modulates plant resistance and cell death in response to Phytophthora parasitica infection

引用

Journal of Integrative Agriculture 2023年第5期22卷 1424-1433页

作者： GAO Xian-xian TANG ya-ling SHI Qing-yao WEI Yu-shu WANG Xiao-xue SHAN Wei-xing QIANG Xiao-yu State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Oomycete, particularly Phytophthora species, causes the most devastating crop diseases, such as potato late blight,and threatens the sustainable crop production worldwide. Our previous studies identified Resistance to Phytophthora parasitica 1(RTP1) as a negative regulator of Arabidopsis resistance to multiple biotrophic pathogens and RTP1 lossof-function plants displayed rapid cell death and reactive oxygen species(ros) production during early colonization of P. parasitica. In this study, we aim to decipher the mechanism of RTP1-mediated cell death, and identify a member of vaculoar processing enzymes(VPEs), γVPE, playing a role in rtp1-mediated resistance to P. parasitica and cell death occurrence. Our results showed up-regulation of the expression of γVPE as well as increased VPE/caspase 1-like protease activity in P. parasitica-infected rtp1 mutant plants. Besides, we found that the VPE/caspase 1-like protease activity was required for the cell death occurrence in Arabidopsis plants during the infection of P. parasitica as well as rtp1-mediated resistance to P. parasitica. Further pathogenicity assays on either Arabidopsis γvpe mutant plants or leaves of Nicotiana benthamiana with transient overexpression of γVPE demonstrated γVPE could positively affect plant resistance to P. parasitica. Together, our studies suggest that γVPE might function as an important regulator of plant defense and cell death occurrence in response to P. parasitica infection, and VPE/caspase 1-like protease activity is required for rtp1-mediated resistance to P. parasitica.

关键词： cell death caspase 1 plant resistance Phytophthora parasitica RTP1 susceptibility factor vacuolar processingenzyme

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：