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自噬途径降解肝脏脂滴的研究进展

临床肝胆病杂志 2024年第9期40卷 1916-1923页

作者：王蓉芝王琳雳焦靖雯于云飞李宝龙黑龙江中医药大学药物安全性评价中心哈尔滨150040

自噬是一种高度保守的细胞降解途径,可通过“脂噬”过程来降解脂滴。脂噬可以选择性地识别脂类物质并将其降解,促进β氧化,进而维持细胞内脂质代谢的平衡状态。肝脏通过脂噬信号通路或关键分子来调控脂滴代谢,进而降低肝脏脂肪变性,改... 详细信息

自噬是一种高度保守的细胞降解途径,可通过“脂噬”过程来降解脂滴。脂噬可以选择性地识别脂类物质并将其降解,促进β氧化,进而维持细胞内脂质代谢的平衡状态。肝脏通过脂噬信号通路或关键分子来调控脂滴代谢,进而降低肝脏脂肪变性,改善非酒精性脂肪性肝病。本文总结归纳了巨自噬、分子伴侣介导的自噬和微自噬样3种自噬途径降解肝脏脂滴的最新研究进展,AMPK/mTOR-ULK1、ATGL-SIRT1、FGF21-JMJD3、Akt作为调控脂噬过程的主要信号通路,有助于维持肝脂质代谢稳态,能够为临床预防和治疗非酒精性脂肪性肝病提供新思路。

关键词：非酒精性脂肪性肝病自噬脂类代谢病理过程

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基于多克隆抗体的糠氨酸ic-ELISA检测方法的建立

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食品与生物技术学报 2024年第5期43卷 138-145页

作者：杨敏仪罗雯倩贾荣业刘宇轩王荣智福建农林大学生命科学学院福建福州350002 福建农林大学生物农药与化学生物学教育部重点实验室福建福州350002 福建农林大学福建省病原真菌与真菌毒素重点实验室福建福州350002

采用甲醛法制备了糠氨酸完全抗原,通过交叉验证确保了多克隆抗体的特异性,从而建立了基于该抗体的糠氨酸间接竞争酶联免疫吸附测定(indirect competitive enzyme-linked immunosorbent assay,ic-ELISA)检测方法。结果显示,建立的ic-ELIS... 详细信息

采用甲醛法制备了糠氨酸完全抗原,通过交叉验证确保了多克隆抗体的特异性,从而建立了基于该抗体的糠氨酸间接竞争酶联免疫吸附测定(indirect competitive enzyme-linked immunosorbent assay,ic-ELISA)检测方法。结果显示,建立的ic-ELISA检测方法有效检测范围为12.20~2143.42 ng/mL,最低检测限为2.69 ng/mL。该方法操作简便、迅速,成本低廉,同时具备高灵敏度和优异特异性,为高效检测食品中糠氨酸质量浓度提供了新的选择,具有一定实际应用价值。

关键词：糠氨酸动物免疫多克隆抗体 ic-ELISA

Temperature-sensitive splicing is an important molecular regulation mechanism of thermosensitive genic male sterility in rice

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Chinese Science Bulletin 2009年第14期54卷 2354-2362页

作者： CHEN rongzhi PAN YuFang wang Yang ZHU LiLi HE GuangCun Key Laboratory of Ministry of Education for Plant Developmental Biology College of Life Sciences Wuhan University Wuhan 430072 China

Photoperiod and temperature-sensitive genetic male sterility (PGMS and TGMS) plants have a number of desirable characteristics for hybrid production. Two-line hybrids developed using the PGMS/TGMS system now account for a large proportion of rice production in China. In this paper, we summarize recent advances on molecular regulation mechanisms and genetics of PGMS/TGMS in rice. We suggest that temperature-sensitive splicing, an important posttranscriptional regulatory mechanism in modulating gene expression and eventually development and differentiation, is also an important molecular regulation mechanism of TGMS in rice. We review those factors involved in temperature-sensitive splicing like cis splice site, snRNA, trans premRNA splicing protein and SR proteins, and delineate that splicing could be regulated by a complex cell signaling pathway. These might shed light on other unknown molecular PGMS/TGMS mechanisms.

关键词：温敏核不育水稻分子调节机制雄性不育基因剪接位点温度敏感分子调控机制信号转导通路 SR蛋白

Detection and identification of Vibrio parahaemolyticus by multiplex PCR and DNA-DNA hybridization on a microarray

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Journal of Genetics and Genomics 2011年第3期38卷 129-135页

作者： rongzhi wang Jiadong Huang Wei Zhang Guangmei Lin Junwei Lian Libin Jiang Hongcong Lin Songfa wang Shihua wang The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology College of Life Sciences Fujian Agriculture and Forestry University Fuzhou 350002 China College of Chemistry and Chemical Engineering University ofJinan Jinan 250022 China

In this paper, we developed a rapid and accurate method for the detection of Vibrio parahaemolyticus strains, using multiplex PCR and DNA--DNA hybridization. Multiplex PCR was used to simultaneously amplify three diagnostic genes （tlh, tdh andfla） that serve as molecular markers of V. parahaemolyticus. Biotinylated PCR products were hybridized to primers immobilized on a microarray, and detected by chemiluminesce with avidin-conjugated alkaline phosphatase. With this method, forty-five samples were tested. Eight known virulent strains （tlh＋/tdh＋/fla＋） and four known avirulent strains （tlh＋/tdh /fla＋） of the V. parahaemolyticus were successfully detected, and no non-specific hybridization and cross-hybridization reaction were found from fifteen closely-related strains （tlh-/tdh-/fla＋） of the Vibrio spp. In addition, all the other eighteen strains of non-Vibrio bacteria （tlh-/tdh /fla-） gave negative results. The DNA microarray successfully distinguished V. parahaemolyticus from other Vibrio spp. The results demonstrated that this was an efficient and robust method for identifying virulent strains of V. parahaemolyticus.

关键词： Vibrio parahaemolyticus Multiplex PCR Hybridization Gene microarray

Interfacial engineering of Ni/V2O3 for hydrogen evolution reaction

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Nano Research 2020年第9期13卷 2407-2412页

作者： Yang Chen Yuan Rao rongzhi wang Yanan Yu Qiulin Li Shujuan Bao Maowen Xu Qin Yue Yanning Zhang Yijin Kang Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China Key Laboratory of Luminescent and Real-Time Analytical Chemistry Ministry of EducationInstitute for Clean Energy and Advanced MaterialsFaculty of Materials and EnergySouthwest UniversityChongqing 400715China

Electrocatalytic water splitting offers a sustainable route for hydrogen production,enabling the clean and renewable alternative energy system of hydrogen *** scarcity and high-cost of platinum-group-metal(PGM)materials urge the exploration of high-performance non-PGM ***,a unique hierarchical structure of NiA/2O3 with extraordinary electrocatalytic performance(e.g.t overpotentials as low as 22 mV at 20 mA·cm^-2 and 94 mV at 100 mA·cm^-2)toward hydrogen evolution reaction in alkaline electrolyte(1 M KOH)is *** investigation on the hierarchical NiA/2O3 with a bimodal size-distribution also offers insight of interfacial engineering that only proper NiA/2O3 interface can effectively improve H20 adsorption,H20 dissociation as well as H adsorption,for an efficient hydrogen production.

关键词： hydrogen evolution reaction hierarchical materials interface electrocatalysis vanadium oxide

Studies on Silicon-Containing Fragrance Raw Materials( Ⅱ )——Syntheses and Odors of 4-Trimethylsilylcyclohex-3-enol and 4-Trimethylsilylcyclohexanol Derivatives

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Chemical Research in Chinese Universities 1993年第2期9卷 121-126页

作者： TANG Shi-xiong, XUE Jie-you, WU Jun-jun, CAO Yu-rongzhi Jin-fang wang Xiao-lan (Department of Chemistry, Nankai University, Tianjin, 300071)

1-Substituted- 4-trimethylsilylcyclohex- 3-enol and 1-substituted- 4-trimetnylsilyl-cyclohexanol, 4-trimethylsilylcyclohex-3-enol and 4-trimethylsilylcyclohexanol, and some of their esters and carbon counterparts were synthesized. Structures of the nineteen new compounds were determined by 1H NMR, IR and MS. Their odors are evaluated.

关键词： Silicon-containing fragrance raw materials Syntheses Odor evaluation

Sugar Regulation of Ugpl Gene Expression in Rice

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Sugar Regulation of Ugpl Gene Expression in Rice

Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure

第十二届全国植物基因组学大会

作者： Lili Zhu,Chunxiao Zhang,Yufang Pan,Zhizheng wang,rongzhi Chen~* Wuhan University,Wuhan,Hubei 430072,China

UDP-glucose pyrophosphorylases(UGPases,EC 2.***.7.9) are key enzymes in plant carbohydrate metabolism and cell wall biosynthesis,catalyzing the reversible production of glucose-1-phosphate and UTP from UDP-glucose and *** rice(Oryza sativa L.) genome contains two homologous UGPase genes,Ugp1 and ***1 is globally expressed throughout the plant and is critical for plant growth and pollen *** the study presented here,possible effects of sugars and osmotic on Ugp 1 expression were tested with excised rice ***1 was up-regulated by feeding sucrose,glucose and fructose to excised rice *** sucrose effect on Ugp1 was mimicked by exposure of dark-adapted leaves to *** sugar signaling pathway for Ugp1 expression was dependent on hexokinase,as N-acetyl-glucosamine(NAG,

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Science China(Life Sciences) 2024年第2期67卷 360-378页

作者： Hongxia Ruan Xuejuan Li Lina Zhou Zihan Zheng Rulin Hua Xu wang Yuan wang Yujie Fan Shuwen Guo Lihua wang Shafiq ur Rahman Ziwei wang Yuyuan Wei Shuangyan Yu rongzhi Zhang Qian Cheng Jie Sheng Xue Li Xiaoyan Liu Ruqiang Yuan Xiaoyan Zhang Lihong Chen Guowang Xu Youfei Guan Jing Nie Hongqiang Qin Feng Zheng Advanced Institute for Medical Sciences Dalian Medical UniversityDalian 116044China Department of Nephrology Second HospitalDalian Medical UniversityDalian 116023China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Chongqing International Institute for Immunology Chongqing 401320China Department of Nephrology Shenzhen HospitalSouthern Medical UniversityShenzhen 518101China Wuhu Hospital and Health Science Center East China Normal UniversityShanghai 200241China Peking University First Hospital Peking UniversityBeijing 100034China

Peritoneal fibrosis together with increased capillaries is the primary cause of peritoneal dialysis *** cell loss is an initiating event for peritoneal *** find that the elevated glucose concentrations in peritoneal dialysate drive mesothelial cell pyroptosis in a manner dependent on caspase-3 and Gasdermin E,driving downstream inflammatory responses,including the activation of ***,pyroptosis is associated with elevated vascular endothelial growth factor A and C,two key factors in vascular angiogenesis and lymphatic vessel *** deficiency mice are protected from high glucose induced peritoneal fibrosis and ultrafiltration *** of melatonin abrogates mesothelial cell pyroptosis through a MT1R-mediated action,and successfully reduces peritoneal fibrosis and angiogenesis in an animal model while preserving dialysis ***,melatonin treatment maintains mitochondrial integrity in mesothelial cells,meanwhile activating m TOR signaling through an increase in the glycolysis product dihydroxyacetone *** effects together with quenching free radicals by melatonin help mesothelial cells maintain a relatively stable internal environment in the face of high-glucose ***,Melatonin treatment holds some promise in preserving mesothelium integrity and in decreasing angiogenesis to protect peritoneum function in patients undergoing peritoneal dialysis.

关键词： melatonin mitochondria pyroptosis GSDME peritoneal fibrosis mTOR

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微型自动分类检测输送线实验装置的设计

湖北汽车工业学院学报 2006年第3期20卷 16-19页

作者：王荣志湖北汽车工业学院机械工程系湖北十堰442002

介绍一种适合机电一体化及机电传动与控制教学的综合性、创新性实验装置的设计及其工作原理和实验方法,并给出了PLC控制程序流程图。

关键词：机电传动实验装置可编程控制器