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Identification and antibiotic susceptibility evaluation of Mycoplasma synoviae isolated from chickens in central China

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Animal Diseases 2023年第3期3卷 202-211页

作者： Chun Wang Naji Zhou Haopeng Lu rongkun yang Weitao Cui Qingrong Xu Yuncai Xiao Sishun Hu Rui Zhou Zili Li Zutao Zhou College of Animal Medicine Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Lab of Agricultural Microbiology of State Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Lab of Preventive Veterinary Medicine of Hubei Province Huazhong Agricultural UniversityWuhan 430070HubeiChina JiangSu Ecolovo Group Ltd. Suqian 223833JiangsuChina

Mycoplasma synoviae(***)infections have become an increasingly serious concern in China because they cause huge economic losses to the poultry *** treatment is one of control strategies that can be used to contain clinical outbreaks in ***-free flocks,especially because the bacteria can be transmitted through *** understand *** infection status in farms of central China and the antibiotic susceptibility of the circulating strains in vivo and in vitro,485 samples were collected from five provinces from 2019 to ***-two strains were isolated and *** of the minimum inhibitory concentration(MIC)of eight antibiotics(tylvalosin,tiamulin,tilmicosin,lincomycin,enrofloxacin,chlortetracycline,doxycycline and tylosin)for isolates showed that tylvalosin,doxycycline and tiamulin were effective against 52 clinical isolates(MIC values≤0.0625-0.25μg/mL,≤0.0625-1μg/mL,and 0.25-2μg/mL,respectively).Tilmicosin,enrofloxacin and lincomycin had high MICo values(>32μg/mL).An artificial *** infection model was established in chickens for evaluation of the short-term therapeutic effect of these *** 5 days of medication,doxycycline(200 mg/L)showed a superior ability to inhibit *** compared with other groups,as did tylvalosin(200 mg/L).Furthermore,the therapeutic efficacy of tylvalosin(0.4μg/mL)on intra-embryo-injected *** was higher than that of tiamulin at the same dose.A combination of MiC values determined in vitro and therapeutic effects observed in vivo revealed that tylvalosin and doxycycline had the best therapeutic *** also showed better inhibitory effects on the vertical transmis-sion of *** than tiamulin.

关键词： Mycoplasma synoviae Antibiotics Minimum inhibitory concentration Therapeutic effect Drug evaluation

Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish(Danio rerio)

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Zoological Research 2024年第3期45卷 567-574页

作者： Peng-Cheng Wang Hao Deng Rang Xu Jiu-Lin Du rongkun Tao Department of Pediatric Cardiology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Medical School Kunming University of Science and TechnologyKunmingYunnan 650500China Scientific Research Center Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China University of Chinese Academy of Sciences Beijing 100049China School of Life Science and Technology ShanghaiTech UniversityShanghai 200031China

Most viruses and transposons serve as effective carriers for the introduction of foreign DNA up to 11 kb into vertebrate ***,their activity markedly diminishes with payloads exceeding 11 *** the payload capacity of transposons could facilitate more sophisticated cargo designs,improving the regulation of expression and minimizing mutagenic risks associated with molecular therapeutics,metabolic engineering,and transgenic animal *** this study,we improved the Tol2 transposon by increasing protein expression levels using a translational enhancer(QBI SP163,ST)and enhanced the nuclear targeting ability using the nuclear localization protein H2B(SHT).The modified Tol2 and ST transposon efficiently integrated large DNA cargos into human cell cultures(H1299),comparable to the well-established super PiggyBac ***,mRNA from ST and SHT showed a significant increase in transgene delivery efficiency of large DNA payloads(8 kb,14 kb,and 24 kb)into zebrafish(Danio rerio).This study presents a modified Tol2 transposon as an enhanced nonviral vector for the delivery of large DNA payloads in transgenic applications.

关键词： Zebrafish Tol2 transposase Transgene Large payload Synthetic biology

High-entropy catalysts for electrochemical water-electrolysis of hydrogen evolution and oxygen evolution reactions

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Frontiers in Energy 2024年第3期18卷 265-290页

作者： Simiao SHA Riyue GE Ying LI Julie M.CAIRNEY rongkun ZHENG Sean LI Bin LIU Jiujun ZHANG Wenxian LI School of Materials Science and Engineering Shanghai UniversityShanghai 200444China School of Chemical and Environmental Engineering Anhui Polytechnic UniversityWuhu 241000China Australian Centre for Microscopy and Microanalysis School of AerospaceMechanical and Mechatronic EngineeringThe University of SydneySydney NSW 2006Australia School of Physics The University of SydneySydney NSW 2006Australia School of Materials Science and Engineering UNSW Materials&Manufacturing Futures InstituteUniversity of New South WalesSydney NSW 2052Australia College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China

High entropy materials (HEMs) have developed rapidly in the field of electrocatalytic water-electrolysis for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) due to their unique properties. In particular, HEM catalysts are composed of many elements. Therefore, they have rich active sites and enhanced entropy stability relative to single atoms. In this paper, the preparation strategies and applications of HEM catalysts in electrochemical water-electrolysis are reviewed to explore the stabilization of HEMs and their catalytic mechanisms as well as their application in support green hydrogen production. First, the concept and four characteristics of HEMs are introduced based on entropy and composition. Then, synthetic strategies of HEM catalysts are systematically reviewed in terms of the categories of bottom-up and top-down. The application of HEMs as catalysts for electrochemical water-electrolysis in recent years is emphatically discussed, and the mechanisms of improving the performance of electrocatalysis is expounded by combining theoretical calculation technology and ex-situ/in situ characterization experiments. Finally, the application prospect of HEMs is proposed to conquer the challenges in HEM catalyst fabrications and applications.

关键词： high-entropy electrocatalysis synthetic methods water-electrolysis hydrogen and oxygen evolutions

Piezo1 channel exaggerates ferroptosis of nucleus pulposus cells by mediating mechanical stress-induced iron influx

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Bone Research 2024年第2期12卷 334-348页

作者： Ziqian Xiang Pengfei Zhang Chunwang Jia rongkun Xu Dingren Cao Zhaoning Xu Tingting Lu Jingwei Liu Xiaoxiong Wang Cheng Qiu Wenyang Fu Weiwei Li Lei Cheng Qiang yang Shiqing Feng Lianlei Wang Yunpeng Zhao Xinyu Liu Department of Orthopaedics Qilu Hospital of Shandong UniversityJinan 250012China University of Health and Rehabilitation Sciences Qingdao 226000China Xiangya School of Medicine Central South UniversityChangsha 410013China School of Nursing and Rehabilitation Shandong UniversityJinan 250012China Department of Pediatrics Cangzhou Central HospitalCangzhou 061011China Department of Pediatric Surgery Qilu Hospital of Shandong UniversityJinan 250012China Department of Pathology Qilu Hospital of Shandong UniversityJinan 250012China Department of Spine Surgery Tianjin HospitalTianjin UniversityTianjin 30021China The Second Hospital of Shandong University Cheeloo College of MedicineShandong UniversityJinan 250012China

To date,several molecules have been found to facilitate iron influx,while the types of iron influx channels remain to be ***,Piezo1 channel was identified as a key iron transporter in response to mechanical ***1-mediated iron overload disturbed iron metabolism and exaggerated ferroptosis in nucleus pulposus cells(NPCs).Importantly,Piezo1-induced iron influx was independent of the transferrin receptor(TFRC),a well-recognized iron ***,pharmacological inactivation of Piezo1 profoundly reduced iron accumulation,alleviated mitochondrial ROS,and suppressed ferroptotic alterations in stimulation of mechanical ***,conditional knockout of Piezo1(Col2a1-CreERT Piezo1^(flox/flox))attenuated the mechanical injury-induced intervertebral disc degeneration(IVDD).Notably,the protective effect of Piezo1 deficiency in IVDD was dampened in Piezo1/Gpx4 conditional double knockout(cDKO)mice(Col2a1-CreERT Piezo1^(flox/flox)/Gpx4^(flox/flox)).These findings suggest that Piezo1 is a potential determinant of iron influx,indicating that the Piezo1-iron-ferroptosis axis might shed light on the treatment of mechanical stress-induced diseases.

关键词： stress overload channel

右归丸对骨性关节炎模型大鼠TGF-β1/Smads信号通路的影响

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中国骨质疏松杂志 2021年第10期27卷 1419-1424页

作者：刘洋孙权杨砥秧荣昆徐远坤贵州中医药大学第一附属医院贵州贵阳550002

目的分析右归丸对骨性关节炎大鼠TGF-β1/Smads信号通路的影响。方法将64只成年SD大鼠利用随机数字表法分为4组,每组15只。正常组不做任何处理;模型组、右归丸组、抑制剂组采用经典的Hulth法建立膝骨性关节炎模型,造模6周后,右归丸组灌... 详细信息

目的分析右归丸对骨性关节炎大鼠TGF-β1/Smads信号通路的影响。方法将64只成年SD大鼠利用随机数字表法分为4组,每组15只。正常组不做任何处理;模型组、右归丸组、抑制剂组采用经典的Hulth法建立膝骨性关节炎模型,造模6周后,右归丸组灌服20 g/(kg·d)的右归丸;抑制剂组腹腔注射100μg/(kg·d)的TGF-β1/Smads信号通路抑制剂SB431542,同时灌服20 g/(kg·d)的右归丸;模型组灌服等剂量的生理盐水,连续给药8周。末次给药24 h后取关节软骨组织,制作病理切片观察软骨病理改变,采用ELISA法检测软骨组织中肿瘤坏死因子α与白细胞介素1β水平,免疫组化染色观察软骨组织中Ⅱ型胶原蛋白与基质金属蛋白酶13(MMP-13)阳性表达,采用RT-PCR法检测软骨组织中TGF-β1、Smad2、Smad3 mRNA表达,采用Western blot检测软骨组织中TGF-β1、p-Smad2、p-Smad3蛋白表达。结果与模型组比较,抑制剂组软骨组织Mankin评分降低(P<0.05),软骨组织中肿瘤坏死因子α与白细胞介素1β水平降低(P<0.05),Ⅱ型胶原蛋白阳性表达升高(P<0.05),MMP-13阳性表达降低(P<0.05),TGF-β1、Smad2、Smad3 mRNA表达升高(P<0.05),TGF-β1、p-Smad2、p-Smad3蛋白表达升高(P<0.05)。与抑制剂组比较,右归丸组软骨组织Mankin评分降低(P<0.05),软骨组织中肿瘤坏死因子α与白细胞介素1β水平降低(P<0.05),Ⅱ型胶原蛋白阳性表达升高(P<0.05),MMP-13阳性表达降低(P<0.05),TGF-β1、Smad2、Smad3 mRNA表达升高(P<0.05),TGF-β1、p-Smad2、p-Smad3蛋白表达升高(P<0.05)。结论右归丸对骨性关节炎大鼠软骨组织具有保护作用,其作用机制可能与TGF-β1/Smads信号通路有关。

关键词：右归丸骨性关节炎软骨 TGF-β1/Smads信号通路大鼠

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艾滋病住院患者129例心理问题与护理

基层医学论坛 2009年第9期13卷 240-241页

作者：杨荣坤腾冲县人民医院云南腾冲679100

艾滋病是由人类免疫缺陷病毒引起的,目前尚无法治愈,导致艾滋病患者心理极为脆弱,对周围环境敏感,对生活失去信心,要承受社会歧视的压力,加之难以支付昂贵的医疗费用,极易产生恐惧、焦虑、孤独及悲观失望的心理。不同的性格、文化层次... 详细信息

艾滋病是由人类免疫缺陷病毒引起的,目前尚无法治愈,导致艾滋病患者心理极为脆弱,对周围环境敏感,对生活失去信心,要承受社会歧视的压力,加之难以支付昂贵的医疗费用,极易产生恐惧、焦虑、孤独及悲观失望的心理。不同的性格、文化层次、病情和心理状况,使艾滋病患者有着不同的心理特征。我院对129例住院患者在整体护理基础上,加强心理护理的比重。经过对照观察,结果显示,到位的心理护理有效地提高了患者的生命质量,大大延长了患者的生命。心理护理对艾滋病患者调整心态、增强免疫力、调动患者战胜疾病的信心、发挥其主观能动性、不断消除心理因素对患者的负面影响起着重要的作用。

关键词：艾滋病心理特征心理护理

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某电站公路石梯坎滑坡稳定分析与治理

四川水利 2007年第3期28卷 23-25页

作者：杨荣坤南充水利电力建筑勘察设计研究院四川南充637000

依据岩梯坎滑坡的特征和基本地质条件,分析滑坡的原因,提出技术可行、经济合理的治理方案,达到了治理滑坡的目的,保证了电站进厂公路的正常运行。

关键词：滑坡特征地质条件滑坡原因治理方案石梯坎滑坡

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指向深度学习的高中英语听说学历案设计

英语教师 2023年第14期23卷 140-144页

作者：杨蓉焜杨茜北京学校北京通州101100

在“新课标、新教材、新高考”背景下,针对深度学习这一焦点议题,以及如何化解思维品质发展和语言能力薄弱这对矛盾,提出指向深度学习的学历案设计。在阐释相关定义、作用及策略的基础上,以人教版(2019年)高中《英语》必修三Unit 4 Spac... 详细信息

在“新课标、新教材、新高考”背景下,针对深度学习这一焦点议题,以及如何化解思维品质发展和语言能力薄弱这对矛盾,提出指向深度学习的学历案设计。在阐释相关定义、作用及策略的基础上,以人教版(2019年)高中《英语》必修三Unit 4 Space Exploration Listening and Speaking板块的How to become an astronaut为例,阐述如何基于学历案设计促进学生对英语听说的深度学习,落实学科核心素养。

关键词：深度学习学历案高中英语听说教学

微量Y对FeCrAl合金包壳力学与氧化性能的影响

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核技术 2023年第6期46卷 15-24页

作者：刘洋马海滨吴利翔郭达禧严俊杨荣坤任啟森薛佳祥中广核研究院有限公司ATF研发专项办公室深圳518000 核电站高安全性事故溶错燃料技术工程实验室深圳518000

FeCrAl合金包壳作为事故容错燃料(Accident Tolerant Fuel,ATF)中长期可商用的技术途径得到了广泛关注。本文旨在研究微量Y对FeCrAl合金包壳力学与氧化性能的影响。采用光学显微镜观察FeCrAl和FeCrAlY合金晶粒尺寸和微观形貌。采用爆破... 详细信息

FeCrAl合金包壳作为事故容错燃料(Accident Tolerant Fuel,ATF)中长期可商用的技术途径得到了广泛关注。本文旨在研究微量Y对FeCrAl合金包壳力学与氧化性能的影响。采用光学显微镜观察FeCrAl和FeCrAlY合金晶粒尺寸和微观形貌。采用爆破试验机和热重分析仪开展内压爆破试验和高温水蒸气氧化试验。采用X射线衍射仪(X-ray Diffractometry,XRD)、扫描电子显微镜(Scanning Electron Microscope,SEM)和能量色散谱仪(Energy Dispersive Spectrometer,EDS)观察FeCrAl和FeCrAlY合金氧化产物成分、表面及截面氧化产物形貌,并对其成分进行分析。研究结果表明:微量Y主要固溶到FeCrAl合金包壳基体中,未形成Fe-Y第二相;固溶到FeCrAl合金包壳基体中的Y未对室温到1000℃内压爆破强度和破口形貌产生影响;但添加微量Y显著改善了FeCrAl合金包壳的耐高温水蒸气氧化性能,800℃、1000℃和1200℃水蒸气氧化8 h条件下,氧化增重量分别下降65.1%、60.0%和31.5%。相比于FeCrAl合金包壳表面单一的Al2O3氧化膜,FeCrAlY包壳表面形成了内部应力更低、致密性更高、且与基体结合力良好的含Y复合氧化膜。由此可见,微量Y的添加对FeCrAl合金的内压爆破性能未产生显著影响,但耐高温水蒸气氧化性能得到显著提高。

关键词： FeCrAl 合金包壳内压爆破高温水蒸气氧化氧化增重